Click here to switch to the map view.

The map label for this gene is amiD [H]

Identifier: 83718870

GI number: 83718870

Start: 2870029

End: 2870928

Strand: Reverse

Name: amiD [H]

Synonym: BTH_I2517

Alternate gene names: 83718870

Gene position: 2870928-2870029 (Counterclockwise)

Preceding gene: 83720095

Following gene: 83718667

Centisome position: 75.37

GC content: 69.11

Gene sequence:

>900_bases
ATGAAATTTCCTTTGCGCCATACGCTTCTCGCGAGCCTCAGCGTGCTCGCCGCTGCCTGTACGACGCCCACGCTCGTCAA
TCGCGGCGACTACTACGCCGACACGAGCCTTCACGCGCGCAGCGCCGATTCGCGCATCCGTTTCCTCGTCATGCATTACA
CCGAGATCGGCGAGGCGCAATCGCTGCGCGTGCTGACGCAGGAGAACGTCAGCGCCCATTACGTCGTGCCGGATGCCCCG
CGCACCAAGCGGCACGCGCCCGTCGTCTATCAGCTCGTGCCGGAAAGCGAGCGCGCATGGCACGCGGGCGTGAGCTCGTG
GCAGGGCGCGACCGAGCTGAACGGCGTGTCGATCGGCATCGAGAACGTCAACCGCGGCCCGATCGACACGCCGCACGGCC
GCATGTGGGCGCCGTATCCGCCCGCGCAGGTCGACGCGATCGTTCGGCTTGCGAAGGATATCGTCGCGCGCTACCAGATT
CCGCCGACGCGCGTCGTCGGCCACAGCGACATCGCACCGCAGCGCAAGATCGATCCAGGCCCGCTGTTTCCGTGGCGTGC
GCTCGCGAAGGCGGGCGTCGGCGCGTGGCCGGACGACGAGACGGTGGCCGCGCGCCTCGCCGGCCGCCCGCCGAAGTCGC
TCGTCGACGTGCGCGAGCTGCAACTGAAGCTCGCGCGCTACGGCTACGACGTCGCGACGGACGGCGTGCTCGACGACCGC
ACGCGGCGCGTGTTCGCCGCGTTCCAGATGCATTTTCGTTCGACCGATTACGCGGGCAATCCGGATGCGGAAACCGACGC
GATCGCGCAGGCGCTGCTCGACAAGTACATGCCGTCGCAGCCGGTGCCCGAACGCGACGCGGCGGCGGTCGATGCGGCGA
GCGGCGTGGCCGCGCAGTGA

Upstream 100 bases:

>100_bases
TGCGAAACGCGCAGGAATGCGATCGACGAAGCGTCTAGAATGAAGCGCTCGTGCCGGGACGACCGTCCCGGCGACTGTCG
ATTCACCGAGCGCCGTCATC

Downstream 100 bases:

>100_bases
GCGCTTCGGGCGCTCGCGCGGCAGCGGACGTGTCGGCGTCGGGCAATCGGCAAGTCGAACGATGACGGTGACGCGGCTGA
ACGATTCTCAATCTGAACGA

Product: N-acetylmuramoyl-L-alanine amidase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKFPLRHTLLASLSVLAAACTTPTLVNRGDYYADTSLHARSADSRIRFLVMHYTEIGEAQSLRVLTQENVSAHYVVPDAP
RTKRHAPVVYQLVPESERAWHAGVSSWQGATELNGVSIGIENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYQI
PPTRVVGHSDIAPQRKIDPGPLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
TRRVFAAFQMHFRSTDYAGNPDAETDAIAQALLDKYMPSQPVPERDAAAVDAASGVAAQ

Sequences:

>Translated_299_residues
MKFPLRHTLLASLSVLAAACTTPTLVNRGDYYADTSLHARSADSRIRFLVMHYTEIGEAQSLRVLTQENVSAHYVVPDAP
RTKRHAPVVYQLVPESERAWHAGVSSWQGATELNGVSIGIENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYQI
PPTRVVGHSDIAPQRKIDPGPLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
TRRVFAAFQMHFRSTDYAGNPDAETDAIAQALLDKYMPSQPVPERDAAAVDAASGVAAQ
>Mature_299_residues
MKFPLRHTLLASLSVLAAACTTPTLVNRGDYYADTSLHARSADSRIRFLVMHYTEIGEAQSLRVLTQENVSAHYVVPDAP
RTKRHAPVVYQLVPESERAWHAGVSSWQGATELNGVSIGIENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYQI
PPTRVVGHSDIAPQRKIDPGPLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
TRRVFAAFQMHFRSTDYAGNPDAETDAIAQALLDKYMPSQPVPERDAAAVDAASGVAAQ

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=252, Percent_Identity=53.968253968254, Blast_Score=257, Evalue=7e-70,
Organism=Escherichia coli, GI1786300, Length=116, Percent_Identity=37.9310344827586, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 32716; Mature: 32716

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPLRHTLLASLSVLAAACTTPTLVNRGDYYADTSLHARSADSRIRFLVMHYTEIGEAQ
CCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCEECCCCHHCCCCCCEEEEEEEEHHCCCCH
SLRVLTQENVSAHYVVPDAPRTKRHAPVVYQLVPESERAWHAGVSSWQGATELNGVSIGI
HEEEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHCCHHCCCCCCCCCEEEEE
ENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYQIPPTRVVGHSDIAPQRKIDPG
CCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCC
PLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
CCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TRRVFAAFQMHFRSTDYAGNPDAETDAIAQALLDKYMPSQPVPERDAAAVDAASGVAAQ
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFPLRHTLLASLSVLAAACTTPTLVNRGDYYADTSLHARSADSRIRFLVMHYTEIGEAQ
CCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCEECCCCHHCCCCCCEEEEEEEEHHCCCCH
SLRVLTQENVSAHYVVPDAPRTKRHAPVVYQLVPESERAWHAGVSSWQGATELNGVSIGI
HEEEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHCCHHCCCCCCCCCEEEEE
ENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYQIPPTRVVGHSDIAPQRKIDPG
CCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCC
PLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
CCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TRRVFAAFQMHFRSTDYAGNPDAETDAIAQALLDKYMPSQPVPERDAAAVDAASGVAAQ
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]