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The map label for this gene is gltX [H]

Identifier: 83719945

GI number: 83719945

Start: 2251166

End: 2252575

Strand: Direct

Name: gltX [H]

Synonym: BTH_I1984

Alternate gene names: 83719945

Gene position: 2251166-2252575 (Clockwise)

Preceding gene: 83718648

Following gene: 83719930

Centisome position: 59.1

GC content: 66.81

Gene sequence:

>1410_bases
ATGACCCGTCCTGTACGCACCCGTTTTGCGCCGAGCCCCACCGGCTTCATCCATCTCGGCAACATCCGATCCGCGCTTTA
TCCGTGGGCGTTCGCCCGCAAGATGAAGGGCACGTTCGTGCTGCGGATCGAGGACACCGATGTCGAGCGTTCGAGCCAGG
AGGCGGTCGACGCGATCCTCGAAGGGATGGCGTGGCTCGGCCTCGATTACGACGAAGGCCCGTACTATCAGATGCAGCGG
ATGGATCGCTATCGCGAAGTGCTCGCGCAGATGCAGGAGAAGGGCCTCGTCTACCCGTGCTACATGTCGACCGAAGAGCT
CGACGCGTTGCGCGAGCGCCAGCGCGCGGCGGGCGAGAAGCCGCGCTACGACGGCACGTGGCGCCCGGAGCCGGGCAAGG
TGCTGCCCGAGCCGCCCGCCGGCGTCGCGCCCGTGCTGCGCTTCCGCAATCCGCTGACAGGCACGGTGGCCTGGGACGAT
GCGGTGAAGGGCCGCGTCGAGATCTCGAACGAGGAACTCGACGATCTCGTCGTCGCGCGTCCCGACGGCACGCCGATGTA
CAACTTCTGCGTCGTCGTCGACGATCTCGACATGGGCATCACGCACGTGATCCGCGGCGACGACCACGTGAACAACACGC
CGCGCCAGATCAACATCCTGCGCGCGCTCGGCGGCGAAGTGCCGGTGTACGCGCACTTGCCGACCGTGCTCAACGAGCAG
GGCGAGAAGATGAGCAAGCGTCACGGCGCGATGAGCGTGATGGGCTATCGCGATGCCGGCTATCTGCCTGAGGCGGTGCT
CAACTATCTCGCGCGCCTTGGCTGGTCGCACGGCGACGCGGAGATCTTCACGCGCGAGCAGTTCGTCGAATGGTTCGACC
TCGAGCATCTCGGCAAGTCGCCCGCGCAGTACGACCATAACAAGCTGAACTGGTTGAACAACCATTACATCAAGGAAGCG
GACGACGCGCGTCTCGCCGGGCTGGCGAAGCCGTTCTTCGCGGCGCTCGGCATCGACGCCGGCGCGATCGAGCAGGGGCC
GGACCTCGTAAGCGTGATGGGGCTGATGAAGGACCGCGCGTCGACGGTGAAGGAAATCGCGGAGAATTCGGCGATGTTCT
ACCGTGCGCCCGCGCCCGGCGCGGATGCGCTCGCGCAGCACGTGACCGACGCGGTGCGTCCGGCGCTCGTGGAGTTCGCG
GCGGCGCTGAAGACGGTCGAATGGACGAAGGAGGCGATCGCGGCCGCGCTGAAGGCCGTGCTCGGTGCGCACAAGCTGAA
GATGCCGCAGCTCGCGATGCCGGTGCGCCTGCTCGTGGCGGGCACGACGCACACGCCGTCGATCGACGCGGTGCTGCTGC
TGTTCGGCCGCGACGTCGTCGTGTCGCGTATCGAGGCGGCGCTCGCGTAA

Upstream 100 bases:

>100_bases
CTTTCCGTTCCGGTCGCGTGCACCCCCGGCAATGCCGGGCGGCGTGCGCCGCCGCGTCGTTTCAATTGATGCGCCGCTTG
AGGCGTTCGAGCTATCGTTT

Downstream 100 bases:

>100_bases
GCGCGCGGTACGTTTGCGGGGCGGCGTCGGTCGAAGTAGCGAGCCGATCGAATGCGCGTACGCGGCCTCGCAAAAAATTT
TGCTCGAGACACAAATAGGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAILEGMAWLGLDYDEGPYYQMQR
MDRYREVLAQMQEKGLVYPCYMSTEELDALRERQRAAGEKPRYDGTWRPEPGKVLPEPPAGVAPVLRFRNPLTGTVAWDD
AVKGRVEISNEELDDLVVARPDGTPMYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQ
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFTREQFVEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEA
DDARLAGLAKPFFAALGIDAGAIEQGPDLVSVMGLMKDRASTVKEIAENSAMFYRAPAPGADALAQHVTDAVRPALVEFA
AALKTVEWTKEAIAAALKAVLGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRIEAALA

Sequences:

>Translated_469_residues
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAILEGMAWLGLDYDEGPYYQMQR
MDRYREVLAQMQEKGLVYPCYMSTEELDALRERQRAAGEKPRYDGTWRPEPGKVLPEPPAGVAPVLRFRNPLTGTVAWDD
AVKGRVEISNEELDDLVVARPDGTPMYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQ
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFTREQFVEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEA
DDARLAGLAKPFFAALGIDAGAIEQGPDLVSVMGLMKDRASTVKEIAENSAMFYRAPAPGADALAQHVTDAVRPALVEFA
AALKTVEWTKEAIAAALKAVLGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRIEAALA
>Mature_468_residues
TRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAILEGMAWLGLDYDEGPYYQMQRM
DRYREVLAQMQEKGLVYPCYMSTEELDALRERQRAAGEKPRYDGTWRPEPGKVLPEPPAGVAPVLRFRNPLTGTVAWDDA
VKGRVEISNEELDDLVVARPDGTPMYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQG
EKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFTREQFVEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEAD
DARLAGLAKPFFAALGIDAGAIEQGPDLVSVMGLMKDRASTVKEIAENSAMFYRAPAPGADALAQHVTDAVRPALVEFAA
ALKTVEWTKEAIAAALKAVLGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRIEAALA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=330, Percent_Identity=35.4545454545455, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=49.1416309012876, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=37.9591836734694, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1786895, Length=312, Percent_Identity=22.4358974358974, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=479, Percent_Identity=28.1837160751566, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324540, Length=332, Percent_Identity=35.5421686746988, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321192, Length=233, Percent_Identity=26.1802575107296, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24665321, Length=497, Percent_Identity=28.3702213279678, Blast_Score=207, Evalue=2e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 52046; Mature: 51915

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAIL
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
EGMAWLGLDYDEGPYYQMQRMDRYREVLAQMQEKGLVYPCYMSTEELDALRERQRAAGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPEPPAGVAPVLRFRNPLTGTVAWDDAVKGRVEISNEELDDLVVAR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCEEEEC
PDGTPMYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQ
CCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHH
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFTREQFVEWFDLEHLGKS
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHNKLNWLNNHYIKEADDARLAGLAKPFFAALGIDAGAIEQGPDLVSVMGLMKDRA
CCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
STVKEIAENSAMFYRAPAPGADALAQHVTDAVRPALVEFAAALKTVEWTKEAIAAALKAV
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRIEAALA
HHHHHCCCCHHHCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
TRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAIL
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
EGMAWLGLDYDEGPYYQMQRMDRYREVLAQMQEKGLVYPCYMSTEELDALRERQRAAGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPEPPAGVAPVLRFRNPLTGTVAWDDAVKGRVEISNEELDDLVVAR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCEEEEC
PDGTPMYNFCVVVDDLDMGITHVIRGDDHVNNTPRQINILRALGGEVPVYAHLPTVLNEQ
CCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHH
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFTREQFVEWFDLEHLGKS
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHNKLNWLNNHYIKEADDARLAGLAKPFFAALGIDAGAIEQGPDLVSVMGLMKDRA
CCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
STVKEIAENSAMFYRAPAPGADALAQHVTDAVRPALVEFAAALKTVEWTKEAIAAALKAV
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAHKLKMPQLAMPVRLLVAGTTHTPSIDAVLLLFGRDVVVSRIEAALA
HHHHHCCCCHHHCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA