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The map label for this gene is proP [H]

Identifier: 83719892

GI number: 83719892

Start: 3207918

End: 3209798

Strand: Direct

Name: proP [H]

Synonym: BTH_I2793

Alternate gene names: 83719892

Gene position: 3207918-3209798 (Clockwise)

Preceding gene: 83720697

Following gene: 83720657

Centisome position: 84.21

GC content: 68.9

Gene sequence:

>1881_bases
ATGTTGTCTCGTTCAACGGCCTACGCCTGTTTAATTATTTCGGTCACCCGACCGTTCGGCCCTGTGCCGCGACTGGCCGT
CCGGAATTTCGCTCACAAGGAAGCGTGCCTTCGGGCAGCTTTTTTCGTTTCGACGCCCTCCAAGGGCATTCAACCGGCCG
CGCGCCACGCGACGTGCCGGTTCGCCGACCGTCCCCGTGCGGCGGTCTTTTCTCACGAGTGCAAGTGCGCGACGACCACC
GGGTCCGCGCCGATCGGTTTCCGCCATGGATTAAGCGCTTCATGCGCCCCGCTCACGCTCGCGACCCTTGTCCGATCGCG
AGCCGCACTCTCCGGCAAACCGTGGCGAACCGCGCCCTGTCGCGCGATTCGGCCGCCGTCACAGGAGTTTTCGACCTTGA
CTGCAACACCCGCCCCCTCTACCCCGTCCATCGCGCCCGCCGAAGCGGCGCCCGCCGCCGCGCACGAAATCACCGTCGTC
GACCAGGGGCTCCTGAAACGCGCCGTCGGCGCGATGGCGCTCGGCAACGCGATGGAATGGTTCGACTTCGGCGTCTACAG
CTACATCGCCGTCACGCTCGGCCAGGTGTTCTTCCCGTCGAGCAGCCCGTCCGCGCAGTTGCTCGCGACGTTCGGCACGT
TCGCCGCCGCATTCCTCGTGCGCCCGCTCGGCGGAATGGTGTTCGGCCCGCTCGGCGACCGCATCGGCCGCCAGCGCGTG
CTCGCGATGACGATGATCATGATGGCCGTCGGCACGTTCGCGATCGGCCTCATCCCGAGCTACGACTCGATCGGCCTGCT
CGCGCCCGCGCTGCTCCTCGTCGCGCGCCTCGTGCAGGGCTTCTCGACGGGCGGCGAATACGGCGGCGCGGCGACCTTCA
TCGCCGAGTTCTCGACCGACAAGCGCCGCGGCTTCATGGGCAGCTTCCTAGAGTTCGGCACGCTGATCGGCTATGTGATG
GGCGCGGGCGTCGTCGCGCTGCTGACCGCCTCGCTGTCGCACGACGCGCTGCTGTCGTGGGGCTGGCGCGTGCCGTTCCT
GATCGCCGGCCCGCTCGGCCTGATCGGCCTGTACATCCGGATGAAGCTCGAAGAAACGCCCGCGTTCAAGCGCCAGGCCG
AGGCGCGCGAAGCGCAGGACAAGGCGGTGCCGAAAGCGCATTTCCGTCGGCAGCTCGCACGGCACTGGCGCGCGCTGCTG
CTGTGCGTCGGACTCGTGCTGATCTTCAACGTCACCGACTACATGGCGCTGTCGTACCTGCCGAGCTATCTATCGTCGAC
GCTGCATTTCGACGAGGCGCACGGCCTCGTGCTGATCCTGATCGTGATGGTGCTGATGATGCCGATGACGCTCGCCACGG
GCCGCCTGTCGGACGCCGTCGGCCGCAAGCCGGTGATGCTCGCCGGCTGCATCGGCCTGTTCGCGCTCGCGATTCCCGCG
CTGCTGCTGATCCGCACGGGCGAGACGTCACTCGTGTTCGGCGGCCTGCTGATCCTCGGCGCGCTGCTGTCGTGCTTCAC
GGGCGTGATGCCGTCGGCGCTGCCGGCGCTCTTCCCGACCGAGATCCGCTACGGCGCGCTCGCGATCGGCTTCAACGTGT
CGGTGTCGCTGTTCGGCGGCACGACGCCGCTCGCCGCCGCGTGGCTCGTCGACGCGACGGGCAACCTGATGATGCCCGCG
TACTATCTGATGGGCGCGGCCGTGATCGGCGCGATCTCGGTGATGGCGCTGCCCGAGAGCGCGCGCCAGCCGCTCAAGGG
CTCGCCGCCCGCCGTCGCGTCGCACCGCGAGGCGCATGCGCTCGCGCGCGAGATCAAGCGCCGCGAGGCGGCCGAGCGCG
ACGACGGCGGCTATGCGAGCGCTGCGGCGCTGCGCGCGTGA

Upstream 100 bases:

>100_bases
CCGCGCGACGCCGCGGCAATCGGCGCGATCATACCCCACGCGACGCCCGAAAACCGGCGCTCGAGCGGCAATTTGCGCGA
TCCTCATTCATCGTGCTATC

Downstream 100 bases:

>100_bases
AATTCGATCGCCGCGACGCAGCCGCGTAGGCGCGTCGTTCGATGACGGACCGCGGAGGCGGCCTTGCGATGCCCGACGGG
CGGCGCGGGGCCGCTCCCGC

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MLSRSTAYACLIISVTRPFGPVPRLAVRNFAHKEACLRAAFFVSTPSKGIQPAARHATCRFADRPRAAVFSHECKCATTT
GSAPIGFRHGLSASCAPLTLATLVRSRAALSGKPWRTAPCRAIRPPSQEFSTLTATPAPSTPSIAPAEAAPAAAHEITVV
DQGLLKRAVGAMALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRV
LAMTMIMMAVGTFAIGLIPSYDSIGLLAPALLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVM
GAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEAREAQDKAVPKAHFRRQLARHWRALL
LCVGLVLIFNVTDYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCIGLFALAIPA
LLLIRTGETSLVFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVDATGNLMMPA
YYLMGAAVIGAISVMALPESARQPLKGSPPAVASHREAHALAREIKRREAAERDDGGYASAAALRA

Sequences:

>Translated_626_residues
MLSRSTAYACLIISVTRPFGPVPRLAVRNFAHKEACLRAAFFVSTPSKGIQPAARHATCRFADRPRAAVFSHECKCATTT
GSAPIGFRHGLSASCAPLTLATLVRSRAALSGKPWRTAPCRAIRPPSQEFSTLTATPAPSTPSIAPAEAAPAAAHEITVV
DQGLLKRAVGAMALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRV
LAMTMIMMAVGTFAIGLIPSYDSIGLLAPALLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVM
GAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEAREAQDKAVPKAHFRRQLARHWRALL
LCVGLVLIFNVTDYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCIGLFALAIPA
LLLIRTGETSLVFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVDATGNLMMPA
YYLMGAAVIGAISVMALPESARQPLKGSPPAVASHREAHALAREIKRREAAERDDGGYASAAALRA
>Mature_626_residues
MLSRSTAYACLIISVTRPFGPVPRLAVRNFAHKEACLRAAFFVSTPSKGIQPAARHATCRFADRPRAAVFSHECKCATTT
GSAPIGFRHGLSASCAPLTLATLVRSRAALSGKPWRTAPCRAIRPPSQEFSTLTATPAPSTPSIAPAEAAPAAAHEITVV
DQGLLKRAVGAMALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRV
LAMTMIMMAVGTFAIGLIPSYDSIGLLAPALLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVM
GAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEAREAQDKAVPKAHFRRQLARHWRALL
LCVGLVLIFNVTDYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCIGLFALAIPA
LLLIRTGETSLVFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVDATGNLMMPA
YYLMGAAVIGAISVMALPESARQPLKGSPPAVASHREAHALAREIKRREAAERDDGGYASAAALRA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=472, Percent_Identity=51.271186440678, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1788292, Length=432, Percent_Identity=32.1759259259259, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=34.1463414634146, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1789941, Length=428, Percent_Identity=34.5794392523364, Blast_Score=183, Evalue=4e-47,
Organism=Escherichia coli, GI87082231, Length=210, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082404, Length=365, Percent_Identity=25.4794520547945, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 66248; Mature: 66248

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRSTAYACLIISVTRPFGPVPRLAVRNFAHKEACLRAAFFVSTPSKGIQPAARHATCR
CCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
FADRPRAAVFSHECKCATTTGSAPIGFRHGLSASCAPLTLATLVRSRAALSGKPWRTAPC
CCCCCCHHHHCCCCCEEECCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RAIRPPSQEFSTLTATPAPSTPSIAPAEAAPAAAHEITVVDQGLLKRAVGAMALGNAMEW
CCCCCCCHHHCEEECCCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHCCHHHH
FDFGVYSYIAVTLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHH
LAMTMIMMAVGTFAIGLIPSYDSIGLLAPALLLVARLVQGFSTGGEYGGAATFIAEFSTD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCC
KRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEECHHHHHHHHHH
MKLEETPAFKRQAEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVTDYMALSYL
EEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PSYLSSTLHFDEAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCIGLFALAIPA
HHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHH
LLLIRTGETSLVFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEECEEEEEEECC
TTPLAAAWLVDATGNLMMPAYYLMGAAVIGAISVMALPESARQPLKGSPPAVASHREAHA
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHCHHHHHH
LAREIKRREAAERDDGGYASAAALRA
HHHHHHHHHHCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MLSRSTAYACLIISVTRPFGPVPRLAVRNFAHKEACLRAAFFVSTPSKGIQPAARHATCR
CCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
FADRPRAAVFSHECKCATTTGSAPIGFRHGLSASCAPLTLATLVRSRAALSGKPWRTAPC
CCCCCCHHHHCCCCCEEECCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RAIRPPSQEFSTLTATPAPSTPSIAPAEAAPAAAHEITVVDQGLLKRAVGAMALGNAMEW
CCCCCCCHHHCEEECCCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHCCHHHH
FDFGVYSYIAVTLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHH
LAMTMIMMAVGTFAIGLIPSYDSIGLLAPALLLVARLVQGFSTGGEYGGAATFIAEFSTD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCC
KRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEECHHHHHHHHHH
MKLEETPAFKRQAEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVTDYMALSYL
EEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PSYLSSTLHFDEAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCIGLFALAIPA
HHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHH
LLLIRTGETSLVFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEECEEEEEEECC
TTPLAAAWLVDATGNLMMPAYYLMGAAVIGAISVMALPESARQPLKGSPPAVASHREAHA
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHCHHHHHH
LAREIKRREAAERDDGGYASAAALRA
HHHHHHHHHHCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]