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The map label for this gene is eutR [H]

Identifier: 83719858

GI number: 83719858

Start: 3703420

End: 3704448

Strand: Direct

Name: eutR [H]

Synonym: BTH_I3248

Alternate gene names: 83719858

Gene position: 3703420-3704448 (Clockwise)

Preceding gene: 83720122

Following gene: 83719895

Centisome position: 97.22

GC content: 72.4

Gene sequence:

>1029_bases
ATGGATCACGAGCACGCCGGCGCCGAGACGACGGCGGAGGATACGGGACGCAGCGCGGACGGCGATGGCGGCGCGGCCGG
GCGTGCGCTCGTCAGCGTCGCGCACGACGCCGACGAGCAGGCGCGCAACCTGATCGGCTGGCGCCAGACCTACGACCAGC
TCGCGGCGGGCCGTTTCGTCGGCACGCTAACCGAGCTGCCGCTCGACACGATGAAGGTGTTCCGCGAGACGACGAGCCAC
ACGCTGCGGCAGGCGTGCGAAGTGCGCGGCGATGCGTACTGGTTCGGCATTCCGGTCGCGCGCGACGGCGCGGCGCGCAT
CGATGCGCGGCCGATCGCCGCCGACGCGCTGGCGTTTCGGCCCGGCAATGTCGAATTCGAGCTGCTCACGCCCGCGCAAT
TCTCGATCTACGGTGTCGTCGTGCGCGGCGCGGTGCTGCGCCGCTATGCGGAGGAAGTCGAGCGCTGCGGGCTCGACGAG
CGATTGCCGCTCGTGCCCGTCGTGCGCGTCGGCGAGGCGCGGCTCACGCGGCTTTGCGCGCTGCTCGCGCAGCGTCTCGA
CGACGCGGACGCGATGAGCGCGGCGGGCGCGCCGCTGTCGGACTGCGCGCGCAACGATCTGCAGGCGGAGGTGCTTGCGG
CGTTGTTCGATCTGTGCGCGTCTCCCGCGGCCGATGCGACGATCGAGCATTCGTCGCGGCGGCGCAGGATCGTCGCGGCC
GCGCGCGATTACGTGCTCGCGCACCGCTCGCGGCCCGTCGGCGTGCCGGAGCTGTGCGAGCAACTGCATGTGAGCCGGCG
CACGCTGCAGTACTGCTTCCAGGACGTGCTCGGGATGGCGCCCGCGACCTATCTGCGCGCGCTGCGGCTCAATGGCGTGC
GGCGCGACTTGCGCGGGCGCGCGGCGGATTCGGTGCAGGACGTCGCGGCCGCGTGGGGGTTTTGGCACCTGAGCCAGTTC
GCGACCGATTACCGGCGGATGTTCGGCGCGCGGCCATCGGAGACGCTGCGCGGCGCGCTCGCCTGCTGA

Upstream 100 bases:

>100_bases
GTCGCACCCACATCGGCGATTTTGGCGACGGTACGCTTCGGCATGGGAATTGCGTAATCGGACGGATCGTTTCAGCTTCA
GCGACGCAAAAGGAGGCGCG

Downstream 100 bases:

>100_bases
GGCGGTGAGCGGCGCGCCGGCCGCCGGCGTCGGGCGGCTCGCGGCTTACGAATCGTGGCTCGCGGCTCGCGCGCGGCGTA
CCCGGCGCGCACGCGGAGTC

Product: HTH-type transcriptional regulator eutR

Products: NA

Alternate protein names: Ethanolamine operon regulatory protein [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MDHEHAGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARNLIGWRQTYDQLAAGRFVGTLTELPLDTMKVFRETTSH
TLRQACEVRGDAYWFGIPVARDGAARIDARPIAADALAFRPGNVEFELLTPAQFSIYGVVVRGAVLRRYAEEVERCGLDE
RLPLVPVVRVGEARLTRLCALLAQRLDDADAMSAAGAPLSDCARNDLQAEVLAALFDLCASPAADATIEHSSRRRRIVAA
ARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPATYLRALRLNGVRRDLRGRAADSVQDVAAAWGFWHLSQF
ATDYRRMFGARPSETLRGALAC

Sequences:

>Translated_342_residues
MDHEHAGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARNLIGWRQTYDQLAAGRFVGTLTELPLDTMKVFRETTSH
TLRQACEVRGDAYWFGIPVARDGAARIDARPIAADALAFRPGNVEFELLTPAQFSIYGVVVRGAVLRRYAEEVERCGLDE
RLPLVPVVRVGEARLTRLCALLAQRLDDADAMSAAGAPLSDCARNDLQAEVLAALFDLCASPAADATIEHSSRRRRIVAA
ARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPATYLRALRLNGVRRDLRGRAADSVQDVAAAWGFWHLSQF
ATDYRRMFGARPSETLRGALAC
>Mature_342_residues
MDHEHAGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARNLIGWRQTYDQLAAGRFVGTLTELPLDTMKVFRETTSH
TLRQACEVRGDAYWFGIPVARDGAARIDARPIAADALAFRPGNVEFELLTPAQFSIYGVVVRGAVLRRYAEEVERCGLDE
RLPLVPVVRVGEARLTRLCALLAQRLDDADAMSAAGAPLSDCARNDLQAEVLAALFDLCASPAADATIEHSSRRRRIVAA
ARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPATYLRALRLNGVRRDLRGRAADSVQDVAAAWGFWHLSQF
ATDYRRMFGARPSETLRGALAC

Specific function: Activates the transcription of the eut operon. Also positively regulates its own transcription. Probably binds ethanolamine and vitamin B12 as effectors [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=310, Percent_Identity=40, Blast_Score=205, Evalue=4e-54,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 37359; Mature: 37359

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHEHAGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARNLIGWRQTYDQLAAGRFV
CCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTLTELPLDTMKVFRETTSHTLRQACEVRGDAYWFGIPVARDGAARIDARPIAADALAFR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECCCCCCCCCCCCHHHHHEEC
PGNVEFELLTPAQFSIYGVVVRGAVLRRYAEEVERCGLDERLPLVPVVRVGEARLTRLCA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHH
LLAQRLDDADAMSAAGAPLSDCARNDLQAEVLAALFDLCASPAADATIEHSSRRRRIVAA
HHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPATYLRALRLNGVRRDLRGR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
AADSVQDVAAAWGFWHLSQFATDYRRMFGARPSETLRGALAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MDHEHAGAETTAEDTGRSADGDGGAAGRALVSVAHDADEQARNLIGWRQTYDQLAAGRFV
CCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTLTELPLDTMKVFRETTSHTLRQACEVRGDAYWFGIPVARDGAARIDARPIAADALAFR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECCCCCCCCCCCCHHHHHEEC
PGNVEFELLTPAQFSIYGVVVRGAVLRRYAEEVERCGLDERLPLVPVVRVGEARLTRLCA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHH
LLAQRLDDADAMSAAGAPLSDCARNDLQAEVLAALFDLCASPAADATIEHSSRRRRIVAA
HHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ARDYVLAHRSRPVGVPELCEQLHVSRRTLQYCFQDVLGMAPATYLRALRLNGVRRDLRGR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
AADSVQDVAAAWGFWHLSQFATDYRRMFGARPSETLRGALAC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503 [H]