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The map label for this gene is stp [H]

Identifier: 83720122

GI number: 83720122

Start: 3699420

End: 3701174

Strand: Direct

Name: stp [H]

Synonym: BTH_I3246

Alternate gene names: 83720122

Gene position: 3699420-3701174 (Clockwise)

Preceding gene: 83720538

Following gene: 83719858

Centisome position: 97.12

GC content: 71.68

Gene sequence:

>1755_bases
TTGTCCATATCGCTCCGGTTCCCTTCGTTACCCGAAATTGCGGAATTCAAGTCCGTAATATACGGAATCTTGCGTTGTTT
TTCGTCGATTGAATCCGGATTATCGTTCGTCGCTCGGCCGGAGGGTTCGTCGTCGCAACTGAGGCAGCGGCGCGCCCGCC
GGCGCTTTTCAAACGGAGCGATGATGATGGATCGATCTTTTTCCGCCGCGCGGCTCGCGATACTCGCGGCCGTCTGCCTC
GCCGCGCTCGCGCTGCCGCTCGGCTTCTCGGGCGGCGCGGTTGCGACGCCCGCGATCGGCCGCGATCTCGGCGGCGGCCC
GGTCGCGATGAACTGGGTCACGAACGCGTTCATGCTGACGTTCGGCAGCCTGTTGATGGCGGCGGGCGCGCTCGCCGACC
AGTTCGGCCGCAAGCGCGTGTTCGCGGCGGGCGTTGGCGGCTTCACCGCGTGCTCGCTCGCGCTCGGCTTCGCGCCGTCG
ATCGTCGCCGTCGATTTGCTGCGCGCCGCGCAAGGCGTGGCGGCCGCCGCGGCGCTCGCGGGCGGCACGGCAGCGCTCGC
GCAGGAGTTCGACGGCGCCGCGCGCACCCGTGCGTTCAGCGTGCTCGGCACGACGTTCGGGCTCGGGCTCGCGTTCGGCC
CGGTGCTTGCGGGCGTGCTGATCGAGTGCTTCGGCTGGCGCGCGATCTTCGCGACGGGCGCCGTGGCGGGCGCGCTGTCG
CTCGCGTTCGGCGTGCCGAGGATGCGCGAGTCGCGCGATCCGCAGGCACAGGGCCTTGACTGGGCCGGGACCCTCGCGTT
CACGGCAATGCTGTCGTGTTTCACGTTCGGCGTGATCCAGGCGCAGGAAAGCGGCTGGGCGAGCCCGCTCGTCGCGGCGC
TGCTCGCGGGCGCGGCGCTCTGCGCGATCGCGTTCGCGACGATCGAGGCGCGCGTCGCGCGGCCGATGCTCGATCTGTCG
CTGTTCCGCTACCCGCGTTTCGTCGGCGTGCAGGTGCTGCCGATCGCGACCTGCTGCTGCTACATCGTGCTGCTCGTCGT
GCTGCCGCTGCGCTTCATCGGCATCGACGGCTTCAGCGAAATCGACGCCGGCTGGCTCATGCTCGCGTTGTCCGCGCCGA
TGCTCGTCGTGCCGATGCTCGCGGCGACGCTCACGCGCTGGATGTCGGCGGGCGTGATCTCGGGCATCGGGCTCGTGATC
GCGGCCGCGGGCCTCGCGTGGCTCGGCCGCGCGCTGCACGGCGGCGCGGGCGCGGCGGCGATCGCGCCGATGCTCGCGAT
CGGCATTGGCGCGGGGATGCCGTGGGGGCTGATGGACGGCTTGTCGGTGAGCGTCGTGCCGAAGGAGCGCGCCGGAATGG
CGACGGGCATTTTCAGCACGACGCGCGTCGCCGGCGAGGGGATCGCGCTCGCGATCGTCAATGCGATTCTCGCGACGCTC
GCGCAGCCGCACGTGCGCGACGCGGTGCCGTCGCTCGTCGCGCCCGCCGCCGTGCACGAGGCCGCCGCGCGGCTCGCGAC
GGGCGACCTCGCGCATGCGGCCGCGCTGCTGCCGGGCGTCGCGCGCGACGCGCTGCGGCAAAGCTATGCGGCTGCGTTCG
GCAGCCTGCTCGGCGGGCTGACGGTCGTCACGCTGCTGTGCGCGCTCGCCGCGTTCGCGTTCCTGAGCGGGACTCGCGCG
CAAGACGAGCCGGTGGCCGAACCGGCGAGCGCGGGCGTTCGCCGTGCGCCGACGCTCGATCGTGAGTGCGGATGA

Upstream 100 bases:

>100_bases
CGTCTTGCCGATCGCCGACGCCGATACGCCGAGCTTGCGTCCCGCCGCGACGAAGCTGAGCGTCTCGGCCGTGCGGACGA
ACGCGACGATGCCGTTCAGG

Downstream 100 bases:

>100_bases
AGTGCGAATGAAGTGCGGAGGATAAAGGGAGGCGGCCGCGCGCCGGCCTGGCGCGATGGCCGTGCGGCCAATGTTCGGAG
AGACCCGAGAGATCGGAGAG

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MSISLRFPSLPEIAEFKSVIYGILRCFSSIESGLSFVARPEGSSSQLRQRRARRRFSNGAMMMDRSFSAARLAILAAVCL
AALALPLGFSGGAVATPAIGRDLGGGPVAMNWVTNAFMLTFGSLLMAAGALADQFGRKRVFAAGVGGFTACSLALGFAPS
IVAVDLLRAAQGVAAAAALAGGTAALAQEFDGAARTRAFSVLGTTFGLGLAFGPVLAGVLIECFGWRAIFATGAVAGALS
LAFGVPRMRESRDPQAQGLDWAGTLAFTAMLSCFTFGVIQAQESGWASPLVAALLAGAALCAIAFATIEARVARPMLDLS
LFRYPRFVGVQVLPIATCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLALSAPMLVVPMLAATLTRWMSAGVISGIGLVI
AAAGLAWLGRALHGGAGAAAIAPMLAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVNAILATL
AQPHVRDAVPSLVAPAAVHEAAARLATGDLAHAAALLPGVARDALRQSYAAAFGSLLGGLTVVTLLCALAAFAFLSGTRA
QDEPVAEPASAGVRRAPTLDRECG

Sequences:

>Translated_584_residues
MSISLRFPSLPEIAEFKSVIYGILRCFSSIESGLSFVARPEGSSSQLRQRRARRRFSNGAMMMDRSFSAARLAILAAVCL
AALALPLGFSGGAVATPAIGRDLGGGPVAMNWVTNAFMLTFGSLLMAAGALADQFGRKRVFAAGVGGFTACSLALGFAPS
IVAVDLLRAAQGVAAAAALAGGTAALAQEFDGAARTRAFSVLGTTFGLGLAFGPVLAGVLIECFGWRAIFATGAVAGALS
LAFGVPRMRESRDPQAQGLDWAGTLAFTAMLSCFTFGVIQAQESGWASPLVAALLAGAALCAIAFATIEARVARPMLDLS
LFRYPRFVGVQVLPIATCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLALSAPMLVVPMLAATLTRWMSAGVISGIGLVI
AAAGLAWLGRALHGGAGAAAIAPMLAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVNAILATL
AQPHVRDAVPSLVAPAAVHEAAARLATGDLAHAAALLPGVARDALRQSYAAAFGSLLGGLTVVTLLCALAAFAFLSGTRA
QDEPVAEPASAGVRRAPTLDRECG
>Mature_583_residues
SISLRFPSLPEIAEFKSVIYGILRCFSSIESGLSFVARPEGSSSQLRQRRARRRFSNGAMMMDRSFSAARLAILAAVCLA
ALALPLGFSGGAVATPAIGRDLGGGPVAMNWVTNAFMLTFGSLLMAAGALADQFGRKRVFAAGVGGFTACSLALGFAPSI
VAVDLLRAAQGVAAAAALAGGTAALAQEFDGAARTRAFSVLGTTFGLGLAFGPVLAGVLIECFGWRAIFATGAVAGALSL
AFGVPRMRESRDPQAQGLDWAGTLAFTAMLSCFTFGVIQAQESGWASPLVAALLAGAALCAIAFATIEARVARPMLDLSL
FRYPRFVGVQVLPIATCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLALSAPMLVVPMLAATLTRWMSAGVISGIGLVIA
AAGLAWLGRALHGGAGAAAIAPMLAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVNAILATLA
QPHVRDAVPSLVAPAAVHEAAARLATGDLAHAAALLPGVARDALRQSYAAAFGSLLGGLTVVTLLCALAAFAFLSGTRAQ
DEPVAEPASAGVRRAPTLDRECG

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=392, Percent_Identity=26.7857142857143, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790195, Length=398, Percent_Identity=24.8743718592965, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=257, Percent_Identity=28.7937743190661, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 59537; Mature: 59405

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISLRFPSLPEIAEFKSVIYGILRCFSSIESGLSFVARPEGSSSQLRQRRARRRFSNGA
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCC
MMMDRSFSAARLAILAAVCLAALALPLGFSGGAVATPAIGRDLGGGPVAMNWVTNAFMLT
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHH
FGSLLMAAGALADQFGRKRVFAAGVGGFTACSLALGFAPSIVAVDLLRAAQGVAAAAALA
HHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
GGTAALAQEFDGAARTRAFSVLGTTFGLGLAFGPVLAGVLIECFGWRAIFATGAVAGALS
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFGVPRMRESRDPQAQGLDWAGTLAFTAMLSCFTFGVIQAQESGWASPLVAALLAGAAL
HHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
CAIAFATIEARVARPMLDLSLFRYPRFVGVQVLPIATCCCYIVLLVVLPLRFIGIDGFSE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
IDAGWLMLALSAPMLVVPMLAATLTRWMSAGVISGIGLVIAAAGLAWLGRALHGGAGAAA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IAPMLAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVNAILATL
HHHHHHHHHCCCCCCHHHCCCEEEEECHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
AQPHVRDAVPSLVAPAAVHEAAARLATGDLAHAAALLPGVARDALRQSYAAAFGSLLGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVVTLLCALAAFAFLSGTRAQDEPVAEPASAGVRRAPTLDRECG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SISLRFPSLPEIAEFKSVIYGILRCFSSIESGLSFVARPEGSSSQLRQRRARRRFSNGA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCC
MMMDRSFSAARLAILAAVCLAALALPLGFSGGAVATPAIGRDLGGGPVAMNWVTNAFMLT
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHH
FGSLLMAAGALADQFGRKRVFAAGVGGFTACSLALGFAPSIVAVDLLRAAQGVAAAAALA
HHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
GGTAALAQEFDGAARTRAFSVLGTTFGLGLAFGPVLAGVLIECFGWRAIFATGAVAGALS
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFGVPRMRESRDPQAQGLDWAGTLAFTAMLSCFTFGVIQAQESGWASPLVAALLAGAAL
HHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
CAIAFATIEARVARPMLDLSLFRYPRFVGVQVLPIATCCCYIVLLVVLPLRFIGIDGFSE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
IDAGWLMLALSAPMLVVPMLAATLTRWMSAGVISGIGLVIAAAGLAWLGRALHGGAGAAA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IAPMLAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVNAILATL
HHHHHHHHHCCCCCCHHHCCCEEEEECHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
AQPHVRDAVPSLVAPAAVHEAAARLATGDLAHAAALLPGVARDALRQSYAAAFGSLLGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVVTLLCALAAFAFLSGTRAQDEPVAEPASAGVRRAPTLDRECG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]