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The map label for this gene is yaiM [C]

Identifier: 83719731

GI number: 83719731

Start: 792286

End: 793134

Strand: Direct

Name: yaiM [C]

Synonym: BTH_I0687

Alternate gene names: 83719731

Gene position: 792286-793134 (Clockwise)

Preceding gene: 83721078

Following gene: 83721212

Centisome position: 20.8

GC content: 71.26

Gene sequence:

>849_bases
ATGCTCGAACTCGTTTCTTCGCATGCGTGCCACGGCGGCGAGCAGCGCTTCTATCGGCACGAATCGAAGACGATCGGACT
GCCGATGAAGTTCTCGGTGTACCTGCCGCCGCAGGCGGCGCGCGGCCGCGTGCCGGCGCTGTTCTACCTCGCCGGGCTCA
CGTGCACCGACGAGACGTTCGCGATCAAGGCGGGCGCGCAGCGCTTCGCCGCGCAGCACGGCGTCGCGCTCGTCGCGCCC
GATACGAGCCCGCGCGGCGCGCTCGTGCCGGGCGAGGCGGATGCGTGGGATTTCGGCGTCGGCGCGGGCTTCTACCTGGA
TGCGACCGAGGCGCCGTGGTCCGCGCACTGGCGGATGGAGTCGTACGTCGCCGGCGAGTTGCGCGAGATCGCGGCGGCCG
AGTTGCCGATCGACGGCGCGCGGCTCGGGATCTTCGGCCACTCGATGGGCGGCCACGGCGCGCTCGTGCTCGCGCTGCGT
CATCCGGACGTCTACCGCTCGGTGTCTGCGTTCGCGCCGATCGCCGCGCCGTCGCGCTGCCCGTGGGGCGAGAAGGCGTT
TTCCGGCTATCTGGGCGCCGACCGCGAAGCGTGGCAGCAGCACGACGCGAGCGAGCTCGTCGCGCGCGCCGGCGCGAAGC
GGTTCGCCGAAGGCATCCTGATCGATCAGGGGCTCGCCGACCCGTTCCTGGCGACGCAGCTCAACCCCGACGTGTTCGAG
GCCGCGTGCCGCGCGGCGGGCCAGCCGCTCACGCTGCGCCGTCACGCGGGCTACGATCACGGCTACTACTTCATCTCGAC
GTTCATCGCCGATCACATCGAGCATCACGCGCGCGTGCTCGGCCGATGA

Upstream 100 bases:

>100_bases
CCGCTCGAGAAGATCAACGACGGCTTCGACCTGATGAAGAAGGGCGAATCGATTCGCTCGGTCGTGCTGTACTGATCGGG
GGCCTTGCATGGGGACGACG

Downstream 100 bases:

>100_bases
GCCGGCGCGCGCGATCGGCACGACGAGCGGCGCGGCAAGCGAAAACGGCGAGAATGACGGAAGAAGCCCGCACGGCTCGC
AAGCGCGGTGCGGGCTTCTT

Product: esterase

Products: NA

Alternate protein names: FGH [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLELVSSHACHGGEQRFYRHESKTIGLPMKFSVYLPPQAARGRVPALFYLAGLTCTDETFAIKAGAQRFAAQHGVALVAP
DTSPRGALVPGEADAWDFGVGAGFYLDATEAPWSAHWRMESYVAGELREIAAAELPIDGARLGIFGHSMGGHGALVLALR
HPDVYRSVSAFAPIAAPSRCPWGEKAFSGYLGADREAWQQHDASELVARAGAKRFAEGILIDQGLADPFLATQLNPDVFE
AACRAAGQPLTLRRHAGYDHGYYFISTFIADHIEHHARVLGR

Sequences:

>Translated_282_residues
MLELVSSHACHGGEQRFYRHESKTIGLPMKFSVYLPPQAARGRVPALFYLAGLTCTDETFAIKAGAQRFAAQHGVALVAP
DTSPRGALVPGEADAWDFGVGAGFYLDATEAPWSAHWRMESYVAGELREIAAAELPIDGARLGIFGHSMGGHGALVLALR
HPDVYRSVSAFAPIAAPSRCPWGEKAFSGYLGADREAWQQHDASELVARAGAKRFAEGILIDQGLADPFLATQLNPDVFE
AACRAAGQPLTLRRHAGYDHGYYFISTFIADHIEHHARVLGR
>Mature_282_residues
MLELVSSHACHGGEQRFYRHESKTIGLPMKFSVYLPPQAARGRVPALFYLAGLTCTDETFAIKAGAQRFAAQHGVALVAP
DTSPRGALVPGEADAWDFGVGAGFYLDATEAPWSAHWRMESYVAGELREIAAAELPIDGARLGIFGHSMGGHGALVLALR
HPDVYRSVSAFAPIAAPSRCPWGEKAFSGYLGADREAWQQHDASELVARAGAKRFAEGILIDQGLADPFLATQLNPDVFE
AACRAAGQPLTLRRHAGYDHGYYFISTFIADHIEHHARVLGR

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate [H]

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

Organism=Homo sapiens, GI33413400, Length=281, Percent_Identity=53.3807829181495, Blast_Score=325, Evalue=2e-89,
Organism=Escherichia coli, GI1786551, Length=280, Percent_Identity=48.2142857142857, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788477, Length=279, Percent_Identity=50.179211469534, Blast_Score=274, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17510185, Length=282, Percent_Identity=49.645390070922, Blast_Score=281, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6322393, Length=301, Percent_Identity=38.5382059800665, Blast_Score=204, Evalue=9e-54,
Organism=Drosophila melanogaster, GI45551932, Length=282, Percent_Identity=51.4184397163121, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24648347, Length=282, Percent_Identity=51.4184397163121, Blast_Score=292, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801
- InterPro:   IPR014186 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: =3.1.2.12 [H]

Molecular weight: Translated: 30495; Mature: 30495

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELVSSHACHGGEQRFYRHESKTIGLPMKFSVYLPPQAARGRVPALFYLAGLTCTDETF
CCCCCCCCCCCCCHHHHHHCCCCEECCCEEEEEEECCHHHCCCCCEEEEEECCEECCCCE
AIKAGAQRFAAQHGVALVAPDTSPRGALVPGEADAWDFGVGAGFYLDATEAPWSAHWRME
EEHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHH
SYVAGELREIAAAELPIDGARLGIFGHSMGGHGALVLALRHPDVYRSVSAFAPIAAPSRC
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCC
PWGEKAFSGYLGADREAWQQHDASELVARAGAKRFAEGILIDQGLADPFLATQLNPDVFE
CCCHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCHHHHH
AACRAAGQPLTLRRHAGYDHGYYFISTFIADHIEHHARVLGR
HHHHHCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLELVSSHACHGGEQRFYRHESKTIGLPMKFSVYLPPQAARGRVPALFYLAGLTCTDETF
CCCCCCCCCCCCCHHHHHHCCCCEECCCEEEEEEECCHHHCCCCCEEEEEECCEECCCCE
AIKAGAQRFAAQHGVALVAPDTSPRGALVPGEADAWDFGVGAGFYLDATEAPWSAHWRME
EEHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHH
SYVAGELREIAAAELPIDGARLGIFGHSMGGHGALVLALRHPDVYRSVSAFAPIAAPSRC
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCC
PWGEKAFSGYLGADREAWQQHDASELVARAGAKRFAEGILIDQGLADPFLATQLNPDVFE
CCCHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCHHHHH
AACRAAGQPLTLRRHAGYDHGYYFISTFIADHIEHHARVLGR
HHHHHCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]