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The map label for this gene is flhA [H]

Identifier: 83721078

GI number: 83721078

Start: 791154

End: 792260

Strand: Direct

Name: flhA [H]

Synonym: BTH_I0686

Alternate gene names: 83721078

Gene position: 791154-792260 (Clockwise)

Preceding gene: 83720840

Following gene: 83719731

Centisome position: 20.77

GC content: 65.67

Gene sequence:

>1107_bases
ATGAAGACGAAAGCCGCGATCGCCTGGAAGGCGGGCGAACCGCTGACGATCGAAGAGGTCGATCTCGAGGGCCCGCGCGC
GGGCGAGGTCCTGATCGAAGTGAAGGCCACCGGCATCTGCCACACCGATTACTACACGCTGTCGGGCGCCGACCCCGAAG
GCCTCTTCCCGGCGATCCTCGGGCACGAGGGCGCGGGCGTCGTGGTCGACGTCGGGCCGGGCGTCGGCACGTTGAAGAAG
GGCGATCACGTGATTCCGCTCTACACGCCCGAATGCCGCGAGTGCAAGTTCTGCCTGTCGCGCAAGACGAACCTGTGCCA
GAAGATCCGCGCGACGCAAGGGCGCGGCCTGATGCCCGACGCGACGTCGCGCTTCTCGATCGGCGGCAAGCCGATCTTCC
ACTACATGGGCACGTCGACGTTCTCGAACTACATCGTCGTCCCCGAGATCGCGGTGGCGAAGGTGCGCGAGGACGCGCCG
TTCGACAAGATCTGCTACATCGGCTGCGGCGTGACGACGGGCGTGGGCGCCGTCGTCTACTCGGCGAAGGTCGAGGCGGG
CGCGAACGTCGTCGTGTTCGGCCTGGGCGGCATCGGCCTCAATGTGATCCAGGGCGCGAGGATGGTGGGCGCGGACAAGA
TCATCGGCGTCGACATCAACCCGAAGCGCGTCGAGCTCGCGAGGAAGTTCGGGATGACGCACTTCGTCAACCCGAACGAG
GTCGAGAACGTGGTCGACCACATCGTGCAGCTGACCGACGGCGGCGCCGACTACTCGTTCGAATGCATCGGCAACGTGAA
GGTGATGCGCCAGGCGCTCGAGTGCACGCACAAGGGCTGGGGCCAGTCGTTCATCATCGGCGTCGCGGCGGCGGGCGAGG
AGATCAGCACGCGGCCGTTCCAGCTCGTGACGGGCCGCGAATGGAAGGGCTCCGCGTTCGGCGGCGCGCGCGGGCGCACC
GACGTGCCGAAGATCGTCGACTGGTACATGGAAGGCAAGATCAACATCGACGACCTGATCACGCACACGCTGCCGCTCGA
GAAGATCAACGACGGCTTCGACCTGATGAAGAAGGGCGAATCGATTCGCTCGGTCGTGCTGTACTGA

Upstream 100 bases:

>100_bases
CGCCGATTCCTGCCAATTTCCGCGCCGCAGCCGCCGGCCCGGCCGCGCATGCGGATACACTTGGCGACATTCCACCCAGT
GACTTCTCCGGAGAACACGC

Downstream 100 bases:

>100_bases
TCGGGGGCCTTGCATGGGGACGACGATGCTCGAACTCGTTTCTTCGCATGCGTGCCACGGCGGCGAGCAGCGCTTCTATC
GGCACGAATCGAAGACGATC

Product: glutathione-dependent formaldehyde dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVVVDVGPGVGTLKK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPDATSRFSIGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDINPKRVELARKFGMTHFVNPNE
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLEKINDGFDLMKKGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVVVDVGPGVGTLKK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPDATSRFSIGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDINPKRVELARKFGMTHFVNPNE
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLEKINDGFDLMKKGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVVVDVGPGVGTLKK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPDATSRFSIGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDINPKRVELARKFGMTHFVNPNE
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLEKINDGFDLMKKGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=61.0354223433242, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.0588235294118, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.3414634146341, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=46.070460704607, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.5284552845528, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=43.9024390243902, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=43.9024390243902, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=44.2934782608696, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=44.3213296398892, Blast_Score=326, Evalue=2e-89,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=59.349593495935, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI87082125, Length=379, Percent_Identity=26.9129287598945, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1790045, Length=376, Percent_Identity=26.5957446808511, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786825, Length=401, Percent_Identity=24.6882793017456, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=26.3157894736842, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=25.3602305475504, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI226510992, Length=372, Percent_Identity=24.4623655913978, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87081918, Length=360, Percent_Identity=28.0555555555556, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788075, Length=382, Percent_Identity=27.2251308900524, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790718, Length=326, Percent_Identity=24.2331288343558, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.5231607629428, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.1554959785523, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=53.8666666666667, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17565904, Length=337, Percent_Identity=55.1928783382789, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=28.3378746594005, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71988145, Length=370, Percent_Identity=25.9459459459459, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=330, Percent_Identity=25.7575757575758, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562878, Length=362, Percent_Identity=24.585635359116, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=54.177897574124, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323729, Length=364, Percent_Identity=27.7472527472527, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=371, Percent_Identity=28.8409703504043, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324486, Length=370, Percent_Identity=25.9459459459459, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319621, Length=372, Percent_Identity=27.1505376344086, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=363, Percent_Identity=28.6501377410468, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=363, Percent_Identity=25.8953168044077, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=363, Percent_Identity=28.6501377410468, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319257, Length=366, Percent_Identity=25.9562841530055, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=256, Percent_Identity=28.515625, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=60.3260869565217, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17137530, Length=357, Percent_Identity=26.890756302521, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39606; Mature: 39606

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAIL
CCCCEEEEEECCCCCEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGVVVDVGPGVGTLKKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSIGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCEECCCCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDINPKRVELARKFGMTHFVNPNE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHTLPLEKINDGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCHHHHHCCC
SIRSVVLY
CHHHEECC
>Mature Secondary Structure
MKTKAAIAWKAGEPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAIL
CCCCEEEEEECCCCCEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGVVVDVGPGVGTLKKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGRGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSIGGKPIFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCEECCCCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGARMVGADKIIGVDINPKRVELARKFGMTHFVNPNE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECIGNVKVMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHTLPLEKINDGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCHHHHHCCC
SIRSVVLY
CHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]