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The map label for this gene is puuA [H]

Identifier: 83719641

GI number: 83719641

Start: 2053555

End: 2054925

Strand: Direct

Name: puuA [H]

Synonym: BTH_I1828

Alternate gene names: 83719641

Gene position: 2053555-2054925 (Clockwise)

Preceding gene: 83720824

Following gene: 83718550

Centisome position: 53.91

GC content: 64.26

Gene sequence:

>1371_bases
GTGACGGCCGTCGACGCTTATCGAAGAGAGGCTCACATGCAAGACATCGATGATTTTCTGAGGCAGCACCGGATCACCGA
AGTGGAGGCGATCATCCCCGACATGGCGGGCATCGCGCGCGGCAAGATCATTCCGCGCAACAAGTTCGAAACCGGCGAAT
CGATGCGCCTGCCGCAAGCGGTGATGGTGCAGACCGTCACCGGCGACTATCCGGAGGACGGCGCGCTCACGGGCGTCACC
GATCCCGACATGGTGTGCGTGGCTGACGCGTCGACGATCTGCCTGATTCCGTGGGCGGTCGATCCGACCGCGCAGGTGAT
TCACGATTGCGTGCATTTCGACGGCTCGCCCGTCGAGATCTCGCCGCGCTACGTGCTGCGCCGGGTGCTCGATCTGTATC
GCGCGAAAGGATGGAAGCCCGTCGTCGCGCCGGAGCTCGAGTTCTATCTGGTCGACATGAACAAGGATCCGGACCTGCCG
CTGCGGCCGCCCGTCGGCCGCACGGGGCGTGCGGAGACGGGGCGCCAGTCGTATTCGATCGAGGCGGTCAACGAATTCGA
TCCGCTCTTCGAGGACATCTACGAATACTGCGAGCTGCAGGGGCTCGACATCGACACGCTGATTCACGAGGTCGGCGCCG
CGCAGATGGAGATCAACTTCCTGCACGGCGACGCGCTGTCGCTCGCCGACCAGGTGTTCCTGTTCAAGCGCACCGTGCGC
GAAGCGGCGCTGCGCCACAACATGTACGCAACGTTCATGGCGAAGCCGATGGAGAACGAGCCGGGTTCGGCGATGCACAT
TCATCAGAGCGTCGTCGACATCGAGACGGGCCGCAACCTCTTCATCGGCGCGAGCGGCGACGTCACGCCGATGTTCCGCG
GCTATCTCGCCGGACTGCAGAAGTACACGCCCGCGCTGATGCCGATCTTCGCGCCGTACATCAACTCGTATCGCCGGCTG
TCGCGCTTCATGGCCGCGCCGATCAACGTGCAGTGGGGTTACGACAACCGCACGGTCGGCTTCCGGATTCCGCATTCGGC
CGCCGCCGCGCGGCGAATCGAGAACCGGATTCCGGGCGTCGACTGCAATCCGTATCTCGCGATCGCGGCCACGCTCGCGG
CCGGCTATCTCGGCGTCACGCAGGCGCTCGAGCCGACCGAGCCGCTCGCGAGCGACGGCTACAGCCTGCCTTATCAACTG
CCGCGCAATCTGGAGGAGGGGCTCACGCTGATGAGCGCGTGCGCGCCGCTCGCCGAGATGCTAGGCGAGAAGTTCGTGAA
GGCCTATCTCGCGTTGAAGGAAACGGAGTACGAAGCATTTTTCCGCGTGATCAGCTCGTGGGAGCGCAAGCACCTGCTGC
TGCACGTTTGA

Upstream 100 bases:

>100_bases
GGCGTTTCGACAGCAATCCGCTGTCGCGAGAAATCTTTGCGGCGTTCGGCGCGGCGTGCCGCGCCCGAAGACGCCGGACG
GCCGGGCGCGCGCCGCGCGC

Downstream 100 bases:

>100_bases
GCGTGCGGGCGCACGCCGCATTCAGGAGGTCAAAGTGACGTATCGAAACGAAGGCATCGCCTATGTCGAGCCGCTGCACG
CGCAGGCGCATGCGCACGCG

Product: glutamine synthetase family protein

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTAVDAYRREAHMQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGALTGVT
DPDMVCVADASTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYRAKGWKPVVAPELEFYLVDMNKDPDLP
LRPPVGRTGRAETGRQSYSIEAVNEFDPLFEDIYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVR
EAALRHNMYATFMAKPMENEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRGYLAGLQKYTPALMPIFAPYINSYRRL
SRFMAAPINVQWGYDNRTVGFRIPHSAAAARRIENRIPGVDCNPYLAIAATLAAGYLGVTQALEPTEPLASDGYSLPYQL
PRNLEEGLTLMSACAPLAEMLGEKFVKAYLALKETEYEAFFRVISSWERKHLLLHV

Sequences:

>Translated_456_residues
MTAVDAYRREAHMQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGALTGVT
DPDMVCVADASTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYRAKGWKPVVAPELEFYLVDMNKDPDLP
LRPPVGRTGRAETGRQSYSIEAVNEFDPLFEDIYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVR
EAALRHNMYATFMAKPMENEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRGYLAGLQKYTPALMPIFAPYINSYRRL
SRFMAAPINVQWGYDNRTVGFRIPHSAAAARRIENRIPGVDCNPYLAIAATLAAGYLGVTQALEPTEPLASDGYSLPYQL
PRNLEEGLTLMSACAPLAEMLGEKFVKAYLALKETEYEAFFRVISSWERKHLLLHV
>Mature_455_residues
TAVDAYRREAHMQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGALTGVTD
PDMVCVADASTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYRAKGWKPVVAPELEFYLVDMNKDPDLPL
RPPVGRTGRAETGRQSYSIEAVNEFDPLFEDIYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVRE
AALRHNMYATFMAKPMENEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRGYLAGLQKYTPALMPIFAPYINSYRRLS
RFMAAPINVQWGYDNRTVGFRIPHSAAAARRIENRIPGVDCNPYLAIAATLAAGYLGVTQALEPTEPLASDGYSLPYQLP
RNLEEGLTLMSACAPLAEMLGEKFVKAYLALKETEYEAFFRVISSWERKHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=445, Percent_Identity=26.9662921348315, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI87081870, Length=456, Percent_Identity=35.3070175438597, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1790301, Length=463, Percent_Identity=28.2937365010799, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 51075; Mature: 50944

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVDAYRREAHMQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQA
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
VMVQTVTGDYPEDGALTGVTDPDMVCVADASTICLIPWAVDPTAQVIHDCVHFDGSPVEI
HHEEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCEEE
SPRYVLRRVLDLYRAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRAETGRQSYSI
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
EAVNEFDPLFEDIYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVR
EEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHH
EAALRHNMYATFMAKPMENEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRGYLAGLQ
HHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
KYTPALMPIFAPYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSAAAARRIENRIPGV
HHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCC
DCNPYLAIAATLAAGYLGVTQALEPTEPLASDGYSLPYQLPRNLEEGLTLMSACAPLAEM
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGEKFVKAYLALKETEYEAFFRVISSWERKHLLLHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure 
TAVDAYRREAHMQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQA
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
VMVQTVTGDYPEDGALTGVTDPDMVCVADASTICLIPWAVDPTAQVIHDCVHFDGSPVEI
HHEEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCEEE
SPRYVLRRVLDLYRAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRAETGRQSYSI
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
EAVNEFDPLFEDIYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVR
EEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHH
EAALRHNMYATFMAKPMENEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRGYLAGLQ
HHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
KYTPALMPIFAPYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSAAAARRIENRIPGV
HHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCC
DCNPYLAIAATLAAGYLGVTQALEPTEPLASDGYSLPYQLPRNLEEGLTLMSACAPLAEM
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGEKFVKAYLALKETEYEAFFRVISSWERKHLLLHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]