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The map label for this gene is argD [C]

Identifier: 83718550

GI number: 83718550

Start: 2054960

End: 2056390

Strand: Direct

Name: argD [C]

Synonym: BTH_I1829

Alternate gene names: 83718550

Gene position: 2054960-2056390 (Clockwise)

Preceding gene: 83719641

Following gene: 83719195

Centisome position: 53.95

GC content: 66.46

Gene sequence:

>1431_bases
GTGACGTATCGAAACGAAGGCATCGCCTATGTCGAGCCGCTGCACGCGCAGGCGCATGCGCACGCGCACCGCACGAGCGC
CGAATATCGCGCGCTCGACGCCGCGCATCACATTCACCCGTTCTCCGACATGGGCTCGCTCAATCGCGCGGGCAGCCGCG
TGATCGTGAAGGCCGAAGGCGTCCATCTGTGGGATTCCGACGGCAACAAGATCATCGACGGCATGGCCGGCCTCTGGTGC
GTGAACGTCGGCTACGGCCGCGAGGAACTGATCGCGGCCGGCAGCCGCCAGTTGCGCGAGCTGCCGTTCTACAACACGTT
CTTCAAGACCACCCATCCGCCCGTCATCGAGCTCTCCGCGCTGCTTGCCGAGATCGCGCCGCCCGCGTTCAACCGCTTCT
TCTATTGCAACAGCGGCTCCGAGGGCAACGATACCGTGCTGCGCATCGCGCATCAGTACTGGCGCACGCAAAACCGGCCG
CAGAAGAAGTTCGTGATCTCGCGCAGGAACGGCTATCACGGCTCGACGATCGCGGGCGCCACGCTCGGCGGCATGGGCTA
CATGCACGAGCAGATGCCCTCGAAGGTCGAGCATATCGTCCACATCGATCAGCCTTATTACTTCGGCGAGGCGAGCGAAG
GGCAGACGCCCGAGGCGTTCGGCCTCGAACGCGCGCGGCAGCTCGAGGCGAAGATTCTCGAGCTCGGCGCGCAGAACGTC
GCCGCGTTCATCGGCGAGCCGTTCCAGGGCGCCGGCGGCGTGATCTTCCCGCCGTCGACGTACTGGCCCGAGATCGAGCG
GATCTGCCGCCAGTACGACGTGCTGCTCGTCGCGGACGAAGTGATCGGCGGCTTCGGGCGCACCGGCGAATGGTTCGCGC
ACCAGCACTTCGGCTTCGAGCCCGACCTGATGACGCTCGCGAAGGGGCTGACGAGCGGTTACGTGCCGATGGGCGCGGTC
GCGCTGTCGGACCGGATCGCCGACGCGCTGATCGCGCACGGCGAGTTCAATCACGGGATGACGTACTCGGGGCATCCGGT
GGCCGCGGCGGTGGCCGTCGCGAATCTGAAGGTGCTGCGCGACGAGAAGATCGTCGAGCGCGTGAAGACCGATACCGGCC
CGTACTTCCAGCAGTTGCTGCGCGAGACGTTCGCGAACCATCCGATCGTCGGGGATATCGCCGGCGCGGGGCTTGTCGCG
GGCATTCAGCTCGCGCGCGAGCCCGGCGCGCGCAAGCGCTTCGAGAACGGCGGCGAGGTCGGCACGATCTGCCGCGACTA
CTGCTTCAACGGCAACCTGATCATGCGCGCGACGGGCGACCGGATGCTGCTTTCGCCGCCGCTCGTGATCTCGCGGCCCG
AAATCGACGAGATCGTCGAGAAGGCGAAGCGTGCGATCGACGCGACAGCGCAGCAACTGGGTCTCGTGTGA

Upstream 100 bases:

>100_bases
GAGTACGAAGCATTTTTCCGCGTGATCAGCTCGTGGGAGCGCAAGCACCTGCTGCTGCACGTTTGAGCGTGCGGGCGCAC
GCCGCATTCAGGAGGTCAAA

Downstream 100 bases:

>100_bases
TGCTGGGCAGGCGGCGTCGGCTGCGGGACGAAGGGGTTTCGTCGGTGGGCATGCGTTTCGGATGCGAGGCGTAGCCTTCA
TACCGCTGCCGGCGTGCGTG

Product: aminotransferase

Products: N-Acetyl-L-glutamate 5-semialdehyde; L-Glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTYRNEGIAYVEPLHAQAHAHAHRTSAEYRALDAAHHIHPFSDMGSLNRAGSRVIVKAEGVHLWDSDGNKIIDGMAGLWC
VNVGYGREELIAAGSRQLRELPFYNTFFKTTHPPVIELSALLAEIAPPAFNRFFYCNSGSEGNDTVLRIAHQYWRTQNRP
QKKFVISRRNGYHGSTIAGATLGGMGYMHEQMPSKVEHIVHIDQPYYFGEASEGQTPEAFGLERARQLEAKILELGAQNV
AAFIGEPFQGAGGVIFPPSTYWPEIERICRQYDVLLVADEVIGGFGRTGEWFAHQHFGFEPDLMTLAKGLTSGYVPMGAV
ALSDRIADALIAHGEFNHGMTYSGHPVAAAVAVANLKVLRDEKIVERVKTDTGPYFQQLLRETFANHPIVGDIAGAGLVA
GIQLAREPGARKRFENGGEVGTICRDYCFNGNLIMRATGDRMLLSPPLVISRPEIDEIVEKAKRAIDATAQQLGLV

Sequences:

>Translated_476_residues
MTYRNEGIAYVEPLHAQAHAHAHRTSAEYRALDAAHHIHPFSDMGSLNRAGSRVIVKAEGVHLWDSDGNKIIDGMAGLWC
VNVGYGREELIAAGSRQLRELPFYNTFFKTTHPPVIELSALLAEIAPPAFNRFFYCNSGSEGNDTVLRIAHQYWRTQNRP
QKKFVISRRNGYHGSTIAGATLGGMGYMHEQMPSKVEHIVHIDQPYYFGEASEGQTPEAFGLERARQLEAKILELGAQNV
AAFIGEPFQGAGGVIFPPSTYWPEIERICRQYDVLLVADEVIGGFGRTGEWFAHQHFGFEPDLMTLAKGLTSGYVPMGAV
ALSDRIADALIAHGEFNHGMTYSGHPVAAAVAVANLKVLRDEKIVERVKTDTGPYFQQLLRETFANHPIVGDIAGAGLVA
GIQLAREPGARKRFENGGEVGTICRDYCFNGNLIMRATGDRMLLSPPLVISRPEIDEIVEKAKRAIDATAQQLGLV
>Mature_475_residues
TYRNEGIAYVEPLHAQAHAHAHRTSAEYRALDAAHHIHPFSDMGSLNRAGSRVIVKAEGVHLWDSDGNKIIDGMAGLWCV
NVGYGREELIAAGSRQLRELPFYNTFFKTTHPPVIELSALLAEIAPPAFNRFFYCNSGSEGNDTVLRIAHQYWRTQNRPQ
KKFVISRRNGYHGSTIAGATLGGMGYMHEQMPSKVEHIVHIDQPYYFGEASEGQTPEAFGLERARQLEAKILELGAQNVA
AFIGEPFQGAGGVIFPPSTYWPEIERICRQYDVLLVADEVIGGFGRTGEWFAHQHFGFEPDLMTLAKGLTSGYVPMGAVA
LSDRIADALIAHGEFNHGMTYSGHPVAAAVAVANLKVLRDEKIVERVKTDTGPYFQQLLRETFANHPIVGDIAGAGLVAG
IQLAREPGARKRFENGGEVGTICRDYCFNGNLIMRATGDRMLLSPPLVISRPEIDEIVEKAKRAIDATAQQLGLV

Specific function: Involved In Both The Arginine And Lysine Biosynthetic Pathways. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI24119277, Length=360, Percent_Identity=29.1666666666667, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI37574042, Length=434, Percent_Identity=26.9585253456221, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI226442705, Length=431, Percent_Identity=27.1461716937355, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI13994255, Length=427, Percent_Identity=27.6346604215457, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI226442709, Length=387, Percent_Identity=27.390180878553, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI4557809, Length=448, Percent_Identity=27.6785714285714, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI284507298, Length=247, Percent_Identity=32.7935222672065, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI188536080, Length=286, Percent_Identity=26.2237762237762, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI38679950, Length=286, Percent_Identity=26.2237762237762, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI38679946, Length=286, Percent_Identity=26.2237762237762, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1789759, Length=413, Percent_Identity=27.6029055690073, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788044, Length=415, Percent_Identity=26.9879518072289, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786991, Length=461, Percent_Identity=28.4164859002169, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI145693181, Length=414, Percent_Identity=25.8454106280193, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1789016, Length=357, Percent_Identity=27.4509803921569, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787560, Length=358, Percent_Identity=29.8882681564246, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786349, Length=307, Percent_Identity=25.7328990228013, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI32564660, Length=444, Percent_Identity=30.4054054054054, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71992977, Length=374, Percent_Identity=29.4117647058824, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI25144271, Length=425, Percent_Identity=27.0588235294118, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25144274, Length=303, Percent_Identity=29.7029702970297, Blast_Score=134, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323470, Length=455, Percent_Identity=27.9120879120879, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324386, Length=368, Percent_Identity=31.25, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324432, Length=432, Percent_Identity=25.462962962963, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321456, Length=355, Percent_Identity=23.0985915492958, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21357415, Length=443, Percent_Identity=27.313769751693, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI161085790, Length=430, Percent_Identity=28.6046511627907, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI28574759, Length=434, Percent_Identity=29.0322580645161, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI21356575, Length=435, Percent_Identity=27.3563218390805, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI281366494, Length=342, Percent_Identity=24.2690058479532, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667139, Length=342, Percent_Identity=24.2690058479532, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667143, Length=342, Percent_Identity=24.2690058479532, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.11 [C]

Molecular weight: Translated: 52349; Mature: 52218

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYRNEGIAYVEPLHAQAHAHAHRTSAEYRALDAAHHIHPFSDMGSLNRAGSRVIVKAEG
CCCCCCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCCCHHCCCCEEEEEECC
VHLWDSDGNKIIDGMAGLWCVNVGYGREELIAAGSRQLRELPFYNTFFKTTHPPVIELSA
EEEECCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHH
LLAEIAPPAFNRFFYCNSGSEGNDTVLRIAHQYWRTQNRPQKKFVISRRNGYHGSTIAGA
HHHHHCCCHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCCCH
TLGGMGYMHEQMPSKVEHIVHIDQPYYFGEASEGQTPEAFGLERARQLEAKILELGAQNV
HHCCHHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHH
AAFIGEPFQGAGGVIFPPSTYWPEIERICRQYDVLLVADEVIGGFGRTGEWFAHQHFGFE
HHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHCEEEEEHHHHCCCCCCCHHHHHHCCCCC
PDLMTLAKGLTSGYVPMGAVALSDRIADALIAHGEFNHGMTYSGHPVAAAVAVANLKVLR
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DEKIVERVKTDTGPYFQQLLRETFANHPIVGDIAGAGLVAGIQLAREPGARKRFENGGEV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEHHCCCHHHHHHHHHCCCCHHHHHCCCCCH
GTICRDYCFNGNLIMRATGDRMLLSPPLVISRPEIDEIVEKAKRAIDATAQQLGLV
HHHHHHHHCCCCEEEEECCCEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TYRNEGIAYVEPLHAQAHAHAHRTSAEYRALDAAHHIHPFSDMGSLNRAGSRVIVKAEG
CCCCCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCCCHHCCCCEEEEEECC
VHLWDSDGNKIIDGMAGLWCVNVGYGREELIAAGSRQLRELPFYNTFFKTTHPPVIELSA
EEEECCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHH
LLAEIAPPAFNRFFYCNSGSEGNDTVLRIAHQYWRTQNRPQKKFVISRRNGYHGSTIAGA
HHHHHCCCHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCCCH
TLGGMGYMHEQMPSKVEHIVHIDQPYYFGEASEGQTPEAFGLERARQLEAKILELGAQNV
HHCCHHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHH
AAFIGEPFQGAGGVIFPPSTYWPEIERICRQYDVLLVADEVIGGFGRTGEWFAHQHFGFE
HHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHCEEEEEHHHHCCCCCCCHHHHHHCCCCC
PDLMTLAKGLTSGYVPMGAVALSDRIADALIAHGEFNHGMTYSGHPVAAAVAVANLKVLR
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DEKIVERVKTDTGPYFQQLLRETFANHPIVGDIAGAGLVAGIQLAREPGARKRFENGGEV
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEHHCCCHHHHHHHHHCCCCHHHHHCCCCCH
GTICRDYCFNGNLIMRATGDRMLLSPPLVISRPEIDEIVEKAKRAIDATAQQLGLV
HHHHHHHHCCCCEEEEECCCEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate [C]

Metal ions: Ni2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.1 {N2-acetyl-L-ornithine}} 1.54 {N2-acetyl-L-ornithine}} 1.3 {N2-acetyl-L-ornithine}} 1.1 {N2-acetyl-L-ornithine}} 0.48 {N2-acetyl-L-ornithine}} 0.34 {N2-acetyl-L-ornithine}} 2.5 {2-oxoglutarate}} [C]

Substrates: N2-Acetyl-L-ornithine; 2-Oxoglutarate [C]

Specific reaction: N2-Acetyl-L-ornithine + 2-Oxoglutarate <==> N-Acetyl-L-glutamate 5-semialdehyde + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: Cu2+; Ornithine; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]