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The map label for this gene is dac [H]

Identifier: 83719526

GI number: 83719526

Start: 771287

End: 772894

Strand: Reverse

Name: dac [H]

Synonym: BTH_I0672

Alternate gene names: 83719526

Gene position: 772894-771287 (Counterclockwise)

Preceding gene: 83718398

Following gene: 83720106

Centisome position: 20.29

GC content: 72.14

Gene sequence:

>1608_bases
GTGCGGCTTGCGTCGCGCGCATGCGCACGCCCCTTTTGCTCACGCCGCCCTCCGTTCCCGTATTCGTCGATGAATCACAA
CGCCGCCCGCATCGCCGCCTCGCCACGTCAGGCCCGTCCCGCCGCCCGCATGCCGCGCGCGCTCCTGCGCGGCGCGATGC
TCGTCGCCGCGTGCGCCGCGCTCGCGTTCGCCGCGCCCGCCGACGCTCGCCGAAAGCCGAAGCCGCCGCGCTACCCCGCC
GCCGTGTCGGCCGCGCGCAACGTGCTGCCCGCGTCGGTGCTCGTCGCGCTGCAGCGCGCGCGCGTGCCGGCGTCGAGCCT
GAGCGTCGTCGTCGAGCGGATCGGCGACTCCACGCCCGTCGTCGCGTGGAACGCGAGCCGGCAGATGCAGCCCGCATCGA
CGATGAAGCTCGTGACGACCTACGCGGGGCTGTCGCTGCTCGGCGCCGACTATCGCTGGCGCACGAGCGCGTACGCGGAC
GGCGGCGTCGACGAGAACGGCACGCTGCACGGCACGCTGTACGTGAAGGGCACGGGCGATCCGAAGCTCGTGCCCGAGGA
ACTGATCGACCTCGTCAACAAGATCCGCCGCGCGGGAATCGTCAACGTCGACGGCGCGCTCGTGCTCGACAAGACCTTCT
TCGCGCCCGAGACGCGCGACCTGCCGCCCCTCGACGACGACGCGAGCGCGCCTTACAACGTCGGCCCCGATCCGCTTCTC
TACGCGTTCAAGGCGCTGTCGTTCACGGTGACGCCGACCGACAACGGCGCGGTCGCGGTCGACGTCGTGCCGCCGCTCGC
GAACCTGACCGTCGACAACCAGTTGGTCGAAGGCCAGGGCTCGTGCGGCGCCGCGCGCCCGACGCTCGTGACCGACGCGA
GCGGCGGGCTCACCGCGTCGTTCGCGGGCGACTATCCGCCGAGCTGCGGCCCGACGACGACCAACCTCGCGGTGCTGAAC
CATTCCGCGTTCTTCGCGCGCGGCTTCCTCGCGCTGTGGCGCCAGACGGGCGGCTCGTTCTCGGGCACGATCTCCGAAGG
CAAGGTGCCGGGCCGCGCGCGGCCCGTTGCGTCGCACCACGGCCCGGTGCTGTCGAGCGTCGTGCACGACATCAACAAGT
TCAGCAACAACGTGATGGCGCGCAACCTGTTCCTCACGATCGGCGCGGTCGAGCACCGGCCGCCCGCGACACCCGCGCAG
TCGGCGGACACGATCCGCGCGTTCCTCGCGCACAGCGGGCTGCCGACCGACGGCCTCACGCTCGAGAACGGCTCGGGGCT
CTCGCGCGACGAACGCGTGAGCGCGCTGTCGCTCGCCGACATGCTGCAGGCGGCGAACGCGAGCCCCGTCGCGCAGGCGT
TCGTCGATTCGCTGCCGATCGCGGGCGTCGACGGCACGATGAAGAACCGGCTCGCGAACGCCCCCGTCGGCGGCAACGCG
CACATCAAGACGGGCACGCTGCGCGACGTGCGCGCGATCGCGGGCTACGTCGCGTCGGCGGACGGGTCGAGCTATGTCGT
CGTCAGCTTCATCAACGACGACCGCGCATCGGCCGCGCGCGCGGCGCACGACGCGCTGCTCGAATGGGTGTACGAAGGGC
CGCGCTGA

Upstream 100 bases:

>100_bases
GTCGCATGCGTTCGAGATGGCGAACATTGTAGCGCCGCCGCGCGCGCAAACGGACCTTCGGCCGATTTCGACGCTGCCCG
CGCGCCGTTTTCTCTACAAT

Downstream 100 bases:

>100_bases
TTCGCGGCCGTACGTGCGGCGGCGCGGCTTTCGCGCCGGCCGCGCGCGGCTAGGAACCGGTTCCGTCCCCGCCGACCCCC
GCAGAATCCGCCCGCATTCG

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein; PBP [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MRLASRACARPFCSRRPPFPYSSMNHNAARIAASPRQARPAARMPRALLRGAMLVAACAALAFAAPADARRKPKPPRYPA
AVSAARNVLPASVLVALQRARVPASSLSVVVERIGDSTPVVAWNASRQMQPASTMKLVTTYAGLSLLGADYRWRTSAYAD
GGVDENGTLHGTLYVKGTGDPKLVPEELIDLVNKIRRAGIVNVDGALVLDKTFFAPETRDLPPLDDDASAPYNVGPDPLL
YAFKALSFTVTPTDNGAVAVDVVPPLANLTVDNQLVEGQGSCGAARPTLVTDASGGLTASFAGDYPPSCGPTTTNLAVLN
HSAFFARGFLALWRQTGGSFSGTISEGKVPGRARPVASHHGPVLSSVVHDINKFSNNVMARNLFLTIGAVEHRPPATPAQ
SADTIRAFLAHSGLPTDGLTLENGSGLSRDERVSALSLADMLQAANASPVAQAFVDSLPIAGVDGTMKNRLANAPVGGNA
HIKTGTLRDVRAIAGYVASADGSSYVVVSFINDDRASAARAAHDALLEWVYEGPR

Sequences:

>Translated_535_residues
MRLASRACARPFCSRRPPFPYSSMNHNAARIAASPRQARPAARMPRALLRGAMLVAACAALAFAAPADARRKPKPPRYPA
AVSAARNVLPASVLVALQRARVPASSLSVVVERIGDSTPVVAWNASRQMQPASTMKLVTTYAGLSLLGADYRWRTSAYAD
GGVDENGTLHGTLYVKGTGDPKLVPEELIDLVNKIRRAGIVNVDGALVLDKTFFAPETRDLPPLDDDASAPYNVGPDPLL
YAFKALSFTVTPTDNGAVAVDVVPPLANLTVDNQLVEGQGSCGAARPTLVTDASGGLTASFAGDYPPSCGPTTTNLAVLN
HSAFFARGFLALWRQTGGSFSGTISEGKVPGRARPVASHHGPVLSSVVHDINKFSNNVMARNLFLTIGAVEHRPPATPAQ
SADTIRAFLAHSGLPTDGLTLENGSGLSRDERVSALSLADMLQAANASPVAQAFVDSLPIAGVDGTMKNRLANAPVGGNA
HIKTGTLRDVRAIAGYVASADGSSYVVVSFINDDRASAARAAHDALLEWVYEGPR
>Mature_535_residues
MRLASRACARPFCSRRPPFPYSSMNHNAARIAASPRQARPAARMPRALLRGAMLVAACAALAFAAPADARRKPKPPRYPA
AVSAARNVLPASVLVALQRARVPASSLSVVVERIGDSTPVVAWNASRQMQPASTMKLVTTYAGLSLLGADYRWRTSAYAD
GGVDENGTLHGTLYVKGTGDPKLVPEELIDLVNKIRRAGIVNVDGALVLDKTFFAPETRDLPPLDDDASAPYNVGPDPLL
YAFKALSFTVTPTDNGAVAVDVVPPLANLTVDNQLVEGQGSCGAARPTLVTDASGGLTASFAGDYPPSCGPTTTNLAVLN
HSAFFARGFLALWRQTGGSFSGTISEGKVPGRARPVASHHGPVLSSVVHDINKFSNNVMARNLFLTIGAVEHRPPATPAQ
SADTIRAFLAHSGLPTDGLTLENGSGLSRDERVSALSLADMLQAANASPVAQAFVDSLPIAGVDGTMKNRLANAPVGGNA
HIKTGTLRDVRAIAGYVASADGSSYVVVSFINDDRASAARAAHDALLEWVYEGPR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=421, Percent_Identity=25.41567695962, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 56038; Mature: 56038

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLASRACARPFCSRRPPFPYSSMNHNAARIAASPRQARPAARMPRALLRGAMLVAACAA
CCCCHHHHHCHHHCCCCCCCCCCCCCCCCEEECCCHHCCCHHHHHHHHHHHHHHHHHHHH
LAFAAPADARRKPKPPRYPAAVSAARNVLPASVLVALQRARVPASSLSVVVERIGDSTPV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCE
VAWNASRQMQPASTMKLVTTYAGLSLLGADYRWRTSAYADGGVDENGTLHGTLYVKGTGD
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCC
PKLVPEELIDLVNKIRRAGIVNVDGALVLDKTFFAPETRDLPPLDDDASAPYNVGPDPLL
CCCCHHHHHHHHHHHHHCCEECCCCEEEEECEECCCCCCCCCCCCCCCCCCCCCCCHHHH
YAFKALSFTVTPTDNGAVAVDVVPPLANLTVDNQLVEGQGSCGAARPTLVTDASGGLTAS
HHHHHHEEEEECCCCCEEEEEECCCCCCCEECCEEECCCCCCCCCCCEEEEECCCCEEEE
FAGDYPPSCGPTTTNLAVLNHSAFFARGFLALWRQTGGSFSGTISEGKVPGRARPVASHH
CCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GPVLSSVVHDINKFSNNVMARNLFLTIGAVEHRPPATPAQSADTIRAFLAHSGLPTDGLT
CHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
LENGSGLSRDERVSALSLADMLQAANASPVAQAFVDSLPIAGVDGTMKNRLANAPVGGNA
ECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCC
HIKTGTLRDVRAIAGYVASADGSSYVVVSFINDDRASAARAAHDALLEWVYEGPR
EEECCCHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLASRACARPFCSRRPPFPYSSMNHNAARIAASPRQARPAARMPRALLRGAMLVAACAA
CCCCHHHHHCHHHCCCCCCCCCCCCCCCCEEECCCHHCCCHHHHHHHHHHHHHHHHHHHH
LAFAAPADARRKPKPPRYPAAVSAARNVLPASVLVALQRARVPASSLSVVVERIGDSTPV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCE
VAWNASRQMQPASTMKLVTTYAGLSLLGADYRWRTSAYADGGVDENGTLHGTLYVKGTGD
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCC
PKLVPEELIDLVNKIRRAGIVNVDGALVLDKTFFAPETRDLPPLDDDASAPYNVGPDPLL
CCCCHHHHHHHHHHHHHCCEECCCCEEEEECEECCCCCCCCCCCCCCCCCCCCCCCHHHH
YAFKALSFTVTPTDNGAVAVDVVPPLANLTVDNQLVEGQGSCGAARPTLVTDASGGLTAS
HHHHHHEEEEECCCCCEEEEEECCCCCCCEECCEEECCCCCCCCCCCEEEEECCCCEEEE
FAGDYPPSCGPTTTNLAVLNHSAFFARGFLALWRQTGGSFSGTISEGKVPGRARPVASHH
CCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GPVLSSVVHDINKFSNNVMARNLFLTIGAVEHRPPATPAQSADTIRAFLAHSGLPTDGLT
CHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
LENGSGLSRDERVSALSLADMLQAANASPVAQAFVDSLPIAGVDGTMKNRLANAPVGGNA
ECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCC
HIKTGTLRDVRAIAGYVASADGSSYVVVSFINDDRASAARAAHDALLEWVYEGPR
EEECCCHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1554361; 7305936 [H]