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The map label for this gene is flgJ [H]

Identifier: 83719486

GI number: 83719486

Start: 280689

End: 281621

Strand: Direct

Name: flgJ [H]

Synonym: BTH_I0248

Alternate gene names: 83719486

Gene position: 280689-281621 (Clockwise)

Preceding gene: 284159410

Following gene: 83720028

Centisome position: 7.37

GC content: 67.31

Gene sequence:

>933_bases
GTGACGACTTTCTCGAAAGCGAACGATCTGTCGCAGCGCTTCGCGCTCGACGTTCAAGGGTTCGACGCGCTGCGCTCGAA
AGCCGCGGCGGTGCCGCCGCGCGAGGGCGTGAAGATGGTCGCGGGCCAGTTCGACGCGATGTTCACGCAGATGATGTTGA
AGAGCATGCGCGATGCGACGCCGTCGGACGGCCTGCTCGATTCGAACTCGTCGAAGATGTACACGTCGATGCTCGATCAG
CAGCTCGCGCAGCAGATGTCGTCGAAGGGCATCGGCGTTGCCGACGCGCTGACGAAGCAGTTGCTGCGCAACGCGAACGT
GGCGCCCGACGCGCAGAGCGAAGGCGGGCTCGCGGCGATGAACGCGCTCGCGAAGGCGTACGCGAATTCGAATGCGTCGT
CCGGCAACGGCGCGCTCGCGGGCACGCACGGCTATTCGGCGGCGAGCGCGCTCACGCCGCCGCTCAAGGGCAACGGCTCC
CCGCAGGCGGAGGCGTTCGTCGAGAAGATGGCGGGCGCCGCGCAGGCGGCGAGCGCGGCCACCGGCATTCCGGCGCGCTT
CATCGTCGGCCAGGCGGCGCTCGAATCCGGCTGGGGCAAGCGCGAGATTCGCGGCGCGAACGGCGAATCGAGCTACAACG
TATTCGGCATCAAGGCGACGAAGGGCTGGACCGGCCGCACGGTGTCGGCCGTCACGACCGAGTACGTGAACGGCAAGCCG
CACCGCGTCGTCGCGCGGTTCCGCGCGTACGATTCGTACGAGCACGCGATGACCGATTACGCGAGCCTCCTGAGGAACAA
CCCGCGCTACGCGAGCGTGCTGAACGCGGGCCACAGCGCGGAGGGCTTCGCCAACGGGATGCAGAAGGCCGGCTACGCGA
CCGATCCGCACTACGCGAAGAAGCTGATTTCGATCATGCAGCAGATCGGCTGA

Upstream 100 bases:

>100_bases
GGCGCTCAACTCGCTCGGCGCGACGCCCGCGGATCTGATGTCGATCCTGCAGGCAATGAAGGCGGCGGGCGCGCTGCGCG
CGGATCTGGAGATCATCTAA

Downstream 100 bases:

>100_bases
TGTAAGCCGAGCAACCGTTCGGTACATTTCGGACCGCGCCGGGCTTCGCCTCGCGCGGTTTTAACGTTTGCTGAAAAAAT
AAACTCCGGTTTCGATCTAA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTTFSKANDLSQRFALDVQGFDALRSKAAAVPPREGVKMVAGQFDAMFTQMMLKSMRDATPSDGLLDSNSSKMYTSMLDQ
QLAQQMSSKGIGVADALTKQLLRNANVAPDAQSEGGLAAMNALAKAYANSNASSGNGALAGTHGYSAASALTPPLKGNGS
PQAEAFVEKMAGAAQAASAATGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGKP
HRVVARFRAYDSYEHAMTDYASLLRNNPRYASVLNAGHSAEGFANGMQKAGYATDPHYAKKLISIMQQIG

Sequences:

>Translated_310_residues
MTTFSKANDLSQRFALDVQGFDALRSKAAAVPPREGVKMVAGQFDAMFTQMMLKSMRDATPSDGLLDSNSSKMYTSMLDQ
QLAQQMSSKGIGVADALTKQLLRNANVAPDAQSEGGLAAMNALAKAYANSNASSGNGALAGTHGYSAASALTPPLKGNGS
PQAEAFVEKMAGAAQAASAATGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGKP
HRVVARFRAYDSYEHAMTDYASLLRNNPRYASVLNAGHSAEGFANGMQKAGYATDPHYAKKLISIMQQIG
>Mature_309_residues
TTFSKANDLSQRFALDVQGFDALRSKAAAVPPREGVKMVAGQFDAMFTQMMLKSMRDATPSDGLLDSNSSKMYTSMLDQQ
LAQQMSSKGIGVADALTKQLLRNANVAPDAQSEGGLAAMNALAKAYANSNASSGNGALAGTHGYSAASALTPPLKGNGSP
QAEAFVEKMAGAAQAASAATGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGKPH
RVVARFRAYDSYEHAMTDYASLLRNNPRYASVLNAGHSAEGFANGMQKAGYATDPHYAKKLISIMQQIG

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=296, Percent_Identity=43.9189189189189, Blast_Score=231, Evalue=5e-62,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 32580; Mature: 32449

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFSKANDLSQRFALDVQGFDALRSKAAAVPPREGVKMVAGQFDAMFTQMMLKSMRDAT
CCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSDGLLDSNSSKMYTSMLDQQLAQQMSSKGIGVADALTKQLLRNANVAPDAQSEGGLAAM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHH
NALAKAYANSNASSGNGALAGTHGYSAASALTPPLKGNGSPQAEAFVEKMAGAAQAASAA
HHHHHHHHCCCCCCCCCCEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGKP
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCC
HRVVARFRAYDSYEHAMTDYASLLRNNPRYASVLNAGHSAEGFANGMQKAGYATDPHYAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
KLISIMQQIG
HHHHHHHHHC
>Mature Secondary Structure 
TTFSKANDLSQRFALDVQGFDALRSKAAAVPPREGVKMVAGQFDAMFTQMMLKSMRDAT
CCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSDGLLDSNSSKMYTSMLDQQLAQQMSSKGIGVADALTKQLLRNANVAPDAQSEGGLAAM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHH
NALAKAYANSNASSGNGALAGTHGYSAASALTPPLKGNGSPQAEAFVEKMAGAAQAASAA
HHHHHHHHCCCCCCCCCCEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGIPARFIVGQAALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGKP
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCC
HRVVARFRAYDSYEHAMTDYASLLRNNPRYASVLNAGHSAEGFANGMQKAGYATDPHYAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHH
KLISIMQQIG
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]