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The map label for this gene is btuF [H]

Identifier: 83719364

GI number: 83719364

Start: 954552

End: 955571

Strand: Reverse

Name: btuF [H]

Synonym: BTH_I0840

Alternate gene names: 83719364

Gene position: 955571-954552 (Counterclockwise)

Preceding gene: 83720683

Following gene: 83719233

Centisome position: 25.09

GC content: 72.35

Gene sequence:

>1020_bases
ATGGCGGCGGCTCGAGCGCGCGTTCGCCGAATGCGTGCCGACGCTGCCCGTCGCCTCGCCGCATCCGTCCGGATCGACGA
CACGAGATTGACGCCCATGCGCCTCGCCCTCTCCCGCGCGCTCCTCGCCGCGATCTTTACCGCATGCGCCTGCGCGCTTG
CGCCCGCCGTCGCGCACGCGGAGCTCGCCGTCACCGACGACGCAGGCCACACGATCACGCTCGCCGCGCCCGCGCGGCGC
GTCGTGAGCCTCGCGCCGCACGTGACCGAGCTGATCTACGCGGCGGGCGGCGGCGCGAAGCTCGTGGGCGCGGTGTCGTA
CAGCGATTACCCGCCCGCCGCGAAGGCGATCCCGCGCGTCGGCTCGAACCAGGCGCTCGACCTCGAACGGATCGCGGCGC
TCAAGCCGGACCTGATCGTCGTCTGGCGTCACGGCAACGCCGGGCGCGAGACCGAGCGGCTGCGCGCGCTCGGCATCCCG
CTCTACTTCAGCGAGCCGAGACATCTCGACGATGTCGCCGCGTCGCTCGACAAACTGGGCACGCTGCTCGGCACGCGCGA
AATCGCGGCGGCGGCGGCGAACGCCTACCGGCAGCAGATCGCGCGGTTGCGCGCGCGCTACGCAGGCAAGCCGCCCGTCA
CCGTGTTCTTTCAGGCCTGGGACAAGCCGCTCATCACGCTGAACGGCGGTCACATCGTCAGCGACGTGATCGCGCTGTGC
GGCGGCCGCAACGTGTTCTCGCAACTGCAGCCGGTCGCGCCGTCCGTCACCGACGAAGCTGTGCTCGCGGCGAATCCGGA
AGCGATCGTGACGACGGCGGCCGGCGCGACCCCGACGCGCGACGCACTACCCGACTTCACGCGCTGGCGCGCGTGGCCGA
AGCTGACCGCGGTCGCGCGAGGCAATCTGTTCGCGATCGACGGCGATCTGCTGACGCGGCCCGCGCCGCGCATCGCGCAA
GGCGCCGAGCAACTGTGCCGGGATCTCGACGTCGCGCGCGCGCGGCGGCCCAAACCGTGA

Upstream 100 bases:

>100_bases
CGCATGCGCTGCATGACGCGCTCGCCGCGCGCGGAATCTGGACGCGCTACTTCGCGCACGCGCCGAGCGTGCGCATCGGG
CTGCCCGCCGGCGAAGACGA

Downstream 100 bases:

>100_bases
GCGCGGCGCGCGCTCGCCAACTCGGCCGAACGAAAATCACGCTCGTGGCAGGCCGGTCGGCCGGTGCTTGAAAGGCCTGC
GCCCAAAAGGGCCGCGCTCG

Product: vitamin B12 transport protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAAARARVRRMRADAARRLAASVRIDDTRLTPMRLALSRALLAAIFTACACALAPAVAHAELAVTDDAGHTITLAAPARR
VVSLAPHVTELIYAAGGGAKLVGAVSYSDYPPAAKAIPRVGSNQALDLERIAALKPDLIVVWRHGNAGRETERLRALGIP
LYFSEPRHLDDVAASLDKLGTLLGTREIAAAAANAYRQQIARLRARYAGKPPVTVFFQAWDKPLITLNGGHIVSDVIALC
GGRNVFSQLQPVAPSVTDEAVLAANPEAIVTTAAGATPTRDALPDFTRWRAWPKLTAVARGNLFAIDGDLLTRPAPRIAQ
GAEQLCRDLDVARARRPKP

Sequences:

>Translated_339_residues
MAAARARVRRMRADAARRLAASVRIDDTRLTPMRLALSRALLAAIFTACACALAPAVAHAELAVTDDAGHTITLAAPARR
VVSLAPHVTELIYAAGGGAKLVGAVSYSDYPPAAKAIPRVGSNQALDLERIAALKPDLIVVWRHGNAGRETERLRALGIP
LYFSEPRHLDDVAASLDKLGTLLGTREIAAAAANAYRQQIARLRARYAGKPPVTVFFQAWDKPLITLNGGHIVSDVIALC
GGRNVFSQLQPVAPSVTDEAVLAANPEAIVTTAAGATPTRDALPDFTRWRAWPKLTAVARGNLFAIDGDLLTRPAPRIAQ
GAEQLCRDLDVARARRPKP
>Mature_338_residues
AAARARVRRMRADAARRLAASVRIDDTRLTPMRLALSRALLAAIFTACACALAPAVAHAELAVTDDAGHTITLAAPARRV
VSLAPHVTELIYAAGGGAKLVGAVSYSDYPPAAKAIPRVGSNQALDLERIAALKPDLIVVWRHGNAGRETERLRALGIPL
YFSEPRHLDDVAASLDKLGTLLGTREIAAAAANAYRQQIARLRARYAGKPPVTVFFQAWDKPLITLNGGHIVSDVIALCG
GRNVFSQLQPVAPSVTDEAVLAANPEAIVTTAAGATPTRDALPDFTRWRAWPKLTAVARGNLFAIDGDLLTRPAPRIAQG
AEQLCRDLDVARARRPKP

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=252, Percent_Identity=37.3015873015873, Blast_Score=154, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 36102; Mature: 35971

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAARARVRRMRADAARRLAASVRIDDTRLTPMRLALSRALLAAIFTACACALAPAVAHA
CCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ELAVTDDAGHTITLAAPARRVVSLAPHVTELIYAAGGGAKLVGAVSYSDYPPAAKAIPRV
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCC
GSNQALDLERIAALKPDLIVVWRHGNAGRETERLRALGIPLYFSEPRHLDDVAASLDKLG
CCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHH
TLLGTREIAAAAANAYRQQIARLRARYAGKPPVTVFFQAWDKPLITLNGGHIVSDVIALC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHH
GGRNVFSQLQPVAPSVTDEAVLAANPEAIVTTAAGATPTRDALPDFTRWRAWPKLTAVAR
CCCHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCHHCCCCHHHHHCCHHHHHEEC
GNLFAIDGDLLTRPAPRIAQGAEQLCRDLDVARARRPKP
CCEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AAARARVRRMRADAARRLAASVRIDDTRLTPMRLALSRALLAAIFTACACALAPAVAHA
CHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ELAVTDDAGHTITLAAPARRVVSLAPHVTELIYAAGGGAKLVGAVSYSDYPPAAKAIPRV
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCC
GSNQALDLERIAALKPDLIVVWRHGNAGRETERLRALGIPLYFSEPRHLDDVAASLDKLG
CCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHHH
TLLGTREIAAAAANAYRQQIARLRARYAGKPPVTVFFQAWDKPLITLNGGHIVSDVIALC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHH
GGRNVFSQLQPVAPSVTDEAVLAANPEAIVTTAAGATPTRDALPDFTRWRAWPKLTAVAR
CCCHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCHHCCCCHHHHHCCHHHHHEEC
GNLFAIDGDLLTRPAPRIAQGAEQLCRDLDVARARRPKP
CCEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA