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The map label for this gene is gatA [H]

Identifier: 83719314

GI number: 83719314

Start: 1817588

End: 1819018

Strand: Reverse

Name: gatA [H]

Synonym: BTH_I1615

Alternate gene names: 83719314

Gene position: 1819018-1817588 (Counterclockwise)

Preceding gene: 83719090

Following gene: 83720270

Centisome position: 47.75

GC content: 74.14

Gene sequence:

>1431_bases
GTGCCGGGCCGCGTCGCACGGCGCTTCGGCAACCCGATCGAGCCTGATACCGCCATGACCACTTTCGCTCCCTTCCCGCC
GCTCGCCCGGCTCGCCGCCGATCTCGCCGCCGGCGCGACGACGAGCCGCTCGCTCGTCGAAGCCGCGCTCGAACGCGCCG
CCGATCCGGCGGGACAAGGCGCGCTCGCGTTCACCGAGATCGACGCGCAAGGCGCGCGCGCGGCCGCCGACGCGCACGAC
CGCCTGCGCGCGGCGGGCACCGTGCTGTCGCCGCTCGCGGGCATTCCGGTGTCGGTGAAGGATCTGTTCGACGTCGCGGG
CCAGGTCACGCGCGCGGGCTCCCGCGTGCTCGCCGACGCGCCCCCTGCGGCGGCCGACGCGATCGCCGTCGCCCGCCTGA
AGCGCGCGGGCGCCGTGCTCGTCGGCCGCACGAACATGAGCGAGTTCGCATTCTCCGGCCTGGGCCTCAATCCGCACTAC
GGCACGCCGCGCTCGCCGTACCGGCGCGGCGTGCCGGGCGACGAGCGGATCGCGGGCGGCTCGTCGTCGGGCGCGGCCGC
ATCGGTTGCGCACGGCATGGCGGCCGTCGCGCTCGCGACCGACACGGGCGGCTCGATCCGGATTCCCGCCGCGTTCTGCG
GGCTGACGGGCTTCAAGCCGACAGCGAGCCGGATTCCGAAGCAAGGCGGCGTGCCACTGTCGACGACGCTCGATTCGTTC
GGCCCGATCGGCGTTTCGGTCGCGTGCTGCGCGCTCGTCGACCGGATTCTCGCGGGCCTCGAGCCGCTCGTGAGCGCCGC
GCGTCCGCTCGAAGGCGTGCGGCTCGGCGTGCTGACGAACTATGTGACGGACGGCGTCGACGCGCACGTCGCGGCATCGC
TCGACGCCGCGCTCAAGCACCTGGAGGCGGCGGGCGCGATCGTGTCGGACGCGCGCTTCGCGCCGCTCGACACGCTGCCC
GACATCAACCGCTACGGTTTTTCTTCGATCGAGGCGTACGCGTGGCATCGCCCGCTCGTCGCGCGCCACCGCGACGCGTA
CGATCCGCGCGTGCTCGCGCGCATCCTGAAGGGCGAGCCGGCGAGCGCCGCCGATTACCTCGACCTCGTCGCCGCGCGCG
CGGCGCTCATCGACGAGGCGGCGCGCACCGTATGGCAGCGCTTCGACGCGCTCGTCGCGCCGACGGTGCCCATCGCGCCG
CCGCGCATCGCCGAACTCGAACGGGACGACGACGCGTTCGCGCGCACGAACGCGCTCGTGCTGCGCAACCCGAGCGCGTT
CAATTTCCTCGACGCGTGCGCGCTGTCGCTGCCGTGCCATCCGCGCGACACGGCGCCCGTCGGCCTGATGCTCGCCGCCG
CGCCGCATCGCGACGACGCGCTGCTCGCGATCGGGCAGGCGGTCGAAGCGGTGCTCGCGACGATCCGCTGA

Upstream 100 bases:

>100_bases
GCGTACCGCCTCGCCCCCCCGCGGCGCACCGCTGTTCTACAATGCGCGAACCCGAGCGCTGCCCGCCGCCGCGCGGCTTC
GCTTCACGAAGCGCCGAACC

Downstream 100 bases:

>100_bases
GCGCCGCCTGCGTGCCGGATGCCGCGCCGTCGGAAAGCGGCGCGGCGTTCGCGTTAAAATCCGACGATTCATTTTCCCGA
ACGCTCGAAGGAATCCCGTT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MPGRVARRFGNPIEPDTAMTTFAPFPPLARLAADLAAGATTSRSLVEAALERAADPAGQGALAFTEIDAQGARAAADAHD
RLRAAGTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLADAPPAAADAIAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHY
GTPRSPYRRGVPGDERIAGGSSSGAAASVAHGMAAVALATDTGGSIRIPAAFCGLTGFKPTASRIPKQGGVPLSTTLDSF
GPIGVSVACCALVDRILAGLEPLVSAARPLEGVRLGVLTNYVTDGVDAHVAASLDAALKHLEAAGAIVSDARFAPLDTLP
DINRYGFSSIEAYAWHRPLVARHRDAYDPRVLARILKGEPASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAP
PRIAELERDDDAFARTNALVLRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHRDDALLAIGQAVEAVLATIR

Sequences:

>Translated_476_residues
MPGRVARRFGNPIEPDTAMTTFAPFPPLARLAADLAAGATTSRSLVEAALERAADPAGQGALAFTEIDAQGARAAADAHD
RLRAAGTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLADAPPAAADAIAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHY
GTPRSPYRRGVPGDERIAGGSSSGAAASVAHGMAAVALATDTGGSIRIPAAFCGLTGFKPTASRIPKQGGVPLSTTLDSF
GPIGVSVACCALVDRILAGLEPLVSAARPLEGVRLGVLTNYVTDGVDAHVAASLDAALKHLEAAGAIVSDARFAPLDTLP
DINRYGFSSIEAYAWHRPLVARHRDAYDPRVLARILKGEPASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAP
PRIAELERDDDAFARTNALVLRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHRDDALLAIGQAVEAVLATIR
>Mature_475_residues
PGRVARRFGNPIEPDTAMTTFAPFPPLARLAADLAAGATTSRSLVEAALERAADPAGQGALAFTEIDAQGARAAADAHDR
LRAAGTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLADAPPAAADAIAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHYG
TPRSPYRRGVPGDERIAGGSSSGAAASVAHGMAAVALATDTGGSIRIPAAFCGLTGFKPTASRIPKQGGVPLSTTLDSFG
PIGVSVACCALVDRILAGLEPLVSAARPLEGVRLGVLTNYVTDGVDAHVAASLDAALKHLEAAGAIVSDARFAPLDTLPD
INRYGFSSIEAYAWHRPLVARHRDAYDPRVLARILKGEPASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAPP
RIAELERDDDAFARTNALVLRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHRDDALLAIGQAVEAVLATIR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=476, Percent_Identity=24.5798319327731, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI222831590, Length=250, Percent_Identity=32.4, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI166795287, Length=138, Percent_Identity=39.1304347826087, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=433, Percent_Identity=25.635103926097, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=303, Percent_Identity=29.7029702970297, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=170, Percent_Identity=37.0588235294118, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=505, Percent_Identity=24.1584158415842, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=234, Percent_Identity=29.4871794871795, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=239, Percent_Identity=31.3807531380753, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=239, Percent_Identity=31.3807531380753, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538254, Length=184, Percent_Identity=30.9782608695652, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=391, Percent_Identity=28.6445012787724, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=153, Percent_Identity=38.562091503268, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=193, Percent_Identity=31.0880829015544, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648113, Length=305, Percent_Identity=33.1147540983607, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550774, Length=217, Percent_Identity=32.7188940092166, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648435, Length=217, Percent_Identity=32.7188940092166, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648437, Length=217, Percent_Identity=32.7188940092166, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648441, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356731, Length=135, Percent_Identity=38.5185185185185, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652985, Length=157, Percent_Identity=31.8471337579618, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922090, Length=157, Percent_Identity=31.8471337579618, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652981, Length=157, Percent_Identity=31.8471337579618, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652983, Length=157, Percent_Identity=31.8471337579618, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49124; Mature: 48993

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGRVARRFGNPIEPDTAMTTFAPFPPLARLAADLAAGATTSRSLVEAALERAADPAGQG
CCCHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
ALAFTEIDAQGARAAADAHDRLRAAGTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLADA
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCC
PPAAADAIAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRGVPGDERIAGG
CCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SSSGAAASVAHGMAAVALATDTGGSIRIPAAFCGLTGFKPTASRIPKQGGVPLSTTLDSF
CCCCCHHHHHHHHEEEEEEECCCCCEECCHHHHCCCCCCCHHHHCCCCCCCCHHHHHHCC
GPIGVSVACCALVDRILAGLEPLVSAARPLEGVRLGVLTNYVTDGVDAHVAASLDAALKH
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEAAGAIVSDARFAPLDTLPDINRYGFSSIEAYAWHRPLVARHRDAYDPRVLARILKGEP
HHHHHHHHHCCCCCCCCCCCCCHHCCCCHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCC
ASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAPPRIAELERDDDAFARTNALV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHCCEEE
LRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHRDDALLAIGQAVEAVLATIR
EECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PGRVARRFGNPIEPDTAMTTFAPFPPLARLAADLAAGATTSRSLVEAALERAADPAGQG
CCHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
ALAFTEIDAQGARAAADAHDRLRAAGTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLADA
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCC
PPAAADAIAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRGVPGDERIAGG
CCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SSSGAAASVAHGMAAVALATDTGGSIRIPAAFCGLTGFKPTASRIPKQGGVPLSTTLDSF
CCCCCHHHHHHHHEEEEEEECCCCCEECCHHHHCCCCCCCHHHHCCCCCCCCHHHHHHCC
GPIGVSVACCALVDRILAGLEPLVSAARPLEGVRLGVLTNYVTDGVDAHVAASLDAALKH
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEAAGAIVSDARFAPLDTLPDINRYGFSSIEAYAWHRPLVARHRDAYDPRVLARILKGEP
HHHHHHHHHCCCCCCCCCCCCCHHCCCCHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCC
ASAADYLDLVAARAALIDEAARTVWQRFDALVAPTVPIAPPRIAELERDDDAFARTNALV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHCCEEE
LRNPSAFNFLDACALSLPCHPRDTAPVGLMLAAAPHRDDALLAIGQAVEAVLATIR
EECCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA