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The map label for this gene is yneJ [H]

Identifier: 83719216

GI number: 83719216

Start: 3033173

End: 3034138

Strand: Direct

Name: yneJ [H]

Synonym: BTH_I2660

Alternate gene names: 83719216

Gene position: 3033173-3034138 (Clockwise)

Preceding gene: 83719189

Following gene: 83721042

Centisome position: 79.63

GC content: 73.6

Gene sequence:

>966_bases
ATGGATCTCGCGGCCCTGGCGATCTTTCGCGCGGTCGTGCGCGAGAACGGCGTGACGCGCGCGGCGGCGAAGCTCAATCG
CGTGCAATCGAACGTCACGACTCGCATCAAGCAACTGGAGGAACAGCTCGGCGCGCCGCTCTTCGTGCGCGATGGCCGCC
GGCTCGTGCTCACGCCCGCCGGCGAGACGCTGCTGCCGTACGCGGAGCGCCTGCTCGCGCTCGCCGACGAGGCGCGCCAC
GCAGTCACCGAAAACCGGCCGCACGGCCGGCTGCGGCTCGGCGCGATGGAAAGCACCGCGGCGAGCCGGCTGCCCGCGTT
TCTCGCCCGCTACCACCAGGCGTGGCCCGACGTCACGCTCGAGCTCGTCACCGGCACGACCGGCAAGCTGATCGGCCTCG
TGCGCGATTTCGAGCTCGACGCCGCGCTCGTCGCGACGCCGGCCGGCTCCGAAGCGCTCGGCGAGCCGTTCGAGACGGCT
TGCGCGTTCACCGAGGAACTCGTGCTGCTGACGCCGCGCGGCCATCCGCGCGTGCGCACGCCGCGCGACGTGCTGCTGCC
GACGCTCGTCGCGTTCGAGCGCGGCTGCGCGTACCGCGGCTGCGTCGAGCGCTGGTACGCGGCGGCTGGGATCAAGCCCG
AGCGCGTGCTCGAACTCGGCTCGTACCATGCGATCGTCGCGTGCGTCGCGGCGGGCGCGGGCGTCGCGGTCGCGCCGCGC
TCGGTGCTCGCCGTCCAGCGCGAGCCGGACAGCCTCGGCGTGCATCCGATCGACGACATGCCGCCGATCGACACGATGCT
CGTCTGGCGTCAAGGACATTTCTCGGCGGCGCTCGCCGCGCTGCGCGATGCATTGCGCGATGCCGCCGCGGCGGCGAACG
CAACCGACGTCGCGGCGCACGAAGCGGCGAAGGTCGCGCGCCGGCGCGCGCGGCGCGGGACGGCGCGCGAGACGCTGTCC
GCGTGA

Upstream 100 bases:

>100_bases
GCAAGATCGCGGTCATGGCCGAAGTCGATTCGATGCTAACTTGACTAATCGTTCTCGAAAAATGAATAATCGAAATTCGA
TCCATCCCACGGAGAGAATC

Downstream 100 bases:

>100_bases
CGCGGGATATGCGCGGATGCATCGTTCGCGTCGCGATCGTCGCGCCGCGACGTGACGCATGCGAGACGTCACGCAAGCAT
CGCCAAGCGCCGCAAACCTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEQLGAPLFVRDGRRLVLTPAGETLLPYAERLLALADEARH
AVTENRPHGRLRLGAMESTAASRLPAFLARYHQAWPDVTLELVTGTTGKLIGLVRDFELDAALVATPAGSEALGEPFETA
CAFTEELVLLTPRGHPRVRTPRDVLLPTLVAFERGCAYRGCVERWYAAAGIKPERVLELGSYHAIVACVAAGAGVAVAPR
SVLAVQREPDSLGVHPIDDMPPIDTMLVWRQGHFSAALAALRDALRDAAAAANATDVAAHEAAKVARRRARRGTARETLS
A

Sequences:

>Translated_321_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEQLGAPLFVRDGRRLVLTPAGETLLPYAERLLALADEARH
AVTENRPHGRLRLGAMESTAASRLPAFLARYHQAWPDVTLELVTGTTGKLIGLVRDFELDAALVATPAGSEALGEPFETA
CAFTEELVLLTPRGHPRVRTPRDVLLPTLVAFERGCAYRGCVERWYAAAGIKPERVLELGSYHAIVACVAAGAGVAVAPR
SVLAVQREPDSLGVHPIDDMPPIDTMLVWRQGHFSAALAALRDALRDAAAAANATDVAAHEAAKVARRRARRGTARETLS
A
>Mature_321_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEQLGAPLFVRDGRRLVLTPAGETLLPYAERLLALADEARH
AVTENRPHGRLRLGAMESTAASRLPAFLARYHQAWPDVTLELVTGTTGKLIGLVRDFELDAALVATPAGSEALGEPFETA
CAFTEELVLLTPRGHPRVRTPRDVLLPTLVAFERGCAYRGCVERWYAAAGIKPERVLELGSYHAIVACVAAGAGVAVAPR
SVLAVQREPDSLGVHPIDDMPPIDTMLVWRQGHFSAALAALRDALRDAAAAANATDVAAHEAAKVARRRARRGTARETLS
A

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=280, Percent_Identity=40.7142857142857, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1788481, Length=295, Percent_Identity=30.8474576271186, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082132, Length=264, Percent_Identity=30.6818181818182, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790208, Length=260, Percent_Identity=32.3076923076923, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=29.6551724137931, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI157672245, Length=261, Percent_Identity=27.9693486590038, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787879, Length=290, Percent_Identity=30, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=28.3400809716599, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994962, Length=113, Percent_Identity=41.5929203539823, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788626, Length=269, Percent_Identity=30.1115241635688, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI2367136, Length=294, Percent_Identity=28.9115646258503, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790262, Length=247, Percent_Identity=25.9109311740891, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787601, Length=176, Percent_Identity=26.7045454545455, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=302, Percent_Identity=28.476821192053, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=250, Percent_Identity=23.6, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789204, Length=247, Percent_Identity=27.5303643724696, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=279, Percent_Identity=24.7311827956989, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786401, Length=131, Percent_Identity=34.3511450381679, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=32.520325203252, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34531; Mature: 34531

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEQLGAPLFVRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECC
GETLLPYAERLLALADEARHAVTENRPHGRLRLGAMESTAASRLPAFLARYHQAWPDVTL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEE
ELVTGTTGKLIGLVRDFELDAALVATPAGSEALGEPFETACAFTEELVLLTPRGHPRVRT
EEEECCCHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHHCCEEEECCCCCCCCCC
PRDVLLPTLVAFERGCAYRGCVERWYAAAGIKPERVLELGSYHAIVACVAAGAGVAVAPR
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCCEECCH
SVLAVQREPDSLGVHPIDDMPPIDTMLVWRQGHFSAALAALRDALRDAAAAANATDVAAH
HHEEEECCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EAAKVARRRARRGTARETLSA
HHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEQLGAPLFVRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECC
GETLLPYAERLLALADEARHAVTENRPHGRLRLGAMESTAASRLPAFLARYHQAWPDVTL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEE
ELVTGTTGKLIGLVRDFELDAALVATPAGSEALGEPFETACAFTEELVLLTPRGHPRVRT
EEEECCCHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHHCCEEEECCCCCCCCCC
PRDVLLPTLVAFERGCAYRGCVERWYAAAGIKPERVLELGSYHAIVACVAAGAGVAVAPR
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCCEECCH
SVLAVQREPDSLGVHPIDDMPPIDTMLVWRQGHFSAALAALRDALRDAAAAANATDVAAH
HHEEEECCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EAAKVARRRARRGTARETLSA
HHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]