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The map label for this gene is garL [H]

Identifier: 83719058

GI number: 83719058

Start: 169476

End: 170261

Strand: Direct

Name: garL [H]

Synonym: BTH_I0140

Alternate gene names: 83719058

Gene position: 169476-170261 (Clockwise)

Preceding gene: 83721427

Following gene: 83721391

Centisome position: 4.45

GC content: 72.9

Gene sequence:

>786_bases
ATGAGCACGCTCACCAATTCCCTGAAGGCGCGTTTGCGCGACGGCGGCGAGCCGCTGCTCGGCCTGTGGTTGACGCTCGC
GAGCGCGGCGGCGACCGAGGCGCTCGCGCACGCCGGCTTCGACTGGCTGTGCATCGACATGGAGCACGCGCCGAACGACA
GCCGCGACGTCGCCTCGCAACTGCGCGCGCTCGCGGCCGCGCATTTGCCGAGCGAGCCCGTCGTGCGCGTGCCGGCGCGC
GAGCCTTGGCTCGTCAAGCGGGCGCTCGACGCGGGCGCGCGCACGCTGATGTTCCCGGGCATCGAGACGGCCGACGAAGC
CGCGCATGCGGTAAGGCTCACCCGCTTTCAGGCGCCCGACGCGCCGGACGGGCTGCGCGGCGTCGCGGGGATCGTCCGCG
CGGCCGCGTACGGGATGCGGCGCGACTACGTGCACACGGCGAACGCGCAGATCGCGACGATCGTGCAGATCGAATCGGCG
CGCGGCATCGACGAAGCCGAGCGGATCGCGGCGACGCCGGGTGTCGACTGCGTGTTTGTCGGGCCTGCCGATCTGTCGGC
GAGCCTCGGGCATCTCGGCGATACGAAGCATCCGGACGTCGCGGCCGCGCTCGAGCGCGTGCTCGCGGCTGGGCGTCGCG
CCGGCGTGCCGGTCGGCATCTTTGCCGCCGACACGGCCGGCGCGCGCCAGTCTATCGACGCGGGATTCCGCGTGGTCGCG
TTGTCGGCGGACGTCGTGTGGCTGCTGCGCGCGACGCGACAGGCGTTGCAGGAGGTGCGAGGATGA

Upstream 100 bases:

>100_bases
CGCGCGCCTGCCTGTTGCGGCGCGGCGCCCGCACGGCGGCCTTGCCGCCCCTCTTTCCGAATCGCAGTCCGCCCGTGCGC
CGCACGACAGGAGGCCGCCG

Downstream 100 bases:

>100_bases
ACGGGAAACGCCGCGGCGCGCCGTTCTCCGTGCGCGCGCGGCACGCGCTTCGCGGCGCGGCGGCGGGTGCGCTCGTCTCG
GCGGCATTCGGGGCGGTGGC

Product: HpcH/HpaI aldolase family protein

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSTLTNSLKARLRDGGEPLLGLWLTLASAAATEALAHAGFDWLCIDMEHAPNDSRDVASQLRALAAAHLPSEPVVRVPAR
EPWLVKRALDAGARTLMFPGIETADEAAHAVRLTRFQAPDAPDGLRGVAGIVRAAAYGMRRDYVHTANAQIATIVQIESA
RGIDEAERIAATPGVDCVFVGPADLSASLGHLGDTKHPDVAAALERVLAAGRRAGVPVGIFAADTAGARQSIDAGFRVVA
LSADVVWLLRATRQALQEVRG

Sequences:

>Translated_261_residues
MSTLTNSLKARLRDGGEPLLGLWLTLASAAATEALAHAGFDWLCIDMEHAPNDSRDVASQLRALAAAHLPSEPVVRVPAR
EPWLVKRALDAGARTLMFPGIETADEAAHAVRLTRFQAPDAPDGLRGVAGIVRAAAYGMRRDYVHTANAQIATIVQIESA
RGIDEAERIAATPGVDCVFVGPADLSASLGHLGDTKHPDVAAALERVLAAGRRAGVPVGIFAADTAGARQSIDAGFRVVA
LSADVVWLLRATRQALQEVRG
>Mature_260_residues
STLTNSLKARLRDGGEPLLGLWLTLASAAATEALAHAGFDWLCIDMEHAPNDSRDVASQLRALAAAHLPSEPVVRVPARE
PWLVKRALDAGARTLMFPGIETADEAAHAVRLTRFQAPDAPDGLRGVAGIVRAAAYGMRRDYVHTANAQIATIVQIESAR
GIDEAERIAATPGVDCVFVGPADLSASLGHLGDTKHPDVAAALERVLAAGRRAGVPVGIFAADTAGARQSIDAGFRVVAL
SADVVWLLRATRQALQEVRG

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=251, Percent_Identity=42.6294820717131, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI1788578, Length=255, Percent_Identity=40, Blast_Score=160, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27454; Mature: 27322

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTNSLKARLRDGGEPLLGLWLTLASAAATEALAHAGFDWLCIDMEHAPNDSRDVASQ
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
LRALAAAHLPSEPVVRVPAREPWLVKRALDAGARTLMFPGIETADEAAHAVRLTRFQAPD
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCC
APDGLRGVAGIVRAAAYGMRRDYVHTANAQIATIVQIESARGIDEAERIAATPGVDCVFV
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCHHHHHHCCCCCCEEEE
GPADLSASLGHLGDTKHPDVAAALERVLAAGRRAGVPVGIFAADTAGARQSIDAGFRVVA
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCHHCCCCEEEEECCCCCCHHHHCCCEEEEE
LSADVVWLLRATRQALQEVRG
ECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLTNSLKARLRDGGEPLLGLWLTLASAAATEALAHAGFDWLCIDMEHAPNDSRDVASQ
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
LRALAAAHLPSEPVVRVPAREPWLVKRALDAGARTLMFPGIETADEAAHAVRLTRFQAPD
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCC
APDGLRGVAGIVRAAAYGMRRDYVHTANAQIATIVQIESARGIDEAERIAATPGVDCVFV
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCHHHHHHCCCCCCEEEE
GPADLSASLGHLGDTKHPDVAAALERVLAAGRRAGVPVGIFAADTAGARQSIDAGFRVVA
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCHHCCCCEEEEECCCCCCHHHHCCCEEEEE
LSADVVWLLRATRQALQEVRG
ECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA