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The map label for this gene is lipB

Identifier: 83719023

GI number: 83719023

Start: 426393

End: 427160

Strand: Direct

Name: lipB

Synonym: BTH_I0385

Alternate gene names: 83719023

Gene position: 426393-427160 (Clockwise)

Preceding gene: 83719944

Following gene: 83720495

Centisome position: 11.19

GC content: 68.49

Gene sequence:

>768_bases
GTGCCTCCCGCCTCCGACGCGCATGCCGCGCCCGACGCCGCCGCGTCCACTTCCGCGTCCCCGCAGTCTTGCGCCGCCCC
GCCTGATACGCTGCCCGTCACGGTCCGCTGGCTCGGCGAGACGCCTTACGACGCATGCTTCGACGCGATGCGCGCGTTCA
CCGACGCGCGCACGCCGGACACCGGCGACGAGATCTGGGTTGTCGAGCACCCGCCCGTCTACACGCTCGGCCAGGCCGGC
AACCCTGCGCACCTGCTCGTCGCCGACAGCGGCGTGCCGCTCGTGAAGGTCGATCGCGGCGGACAAATCACCTACCACGG
TCCGGGGCAAATCGTCGCGTATCTGCTCATCGACTTGAGGCGGCGCAAGCTGATGGTGCGCACGCTCGTCACGCGGATCG
AACAGGCCGTGATCGAGACGCTTGCGGCGTATAATCTCGCGTCGGTCCGCAAGGCCGGCGCGCCCGGGATCTACGTGGAA
TCGGGGCCGCATCATGGCGCGAAGATTGCCGCGCTCGGCCTGAAGATCCGCAACGGCTGCAGCTATCACGGCCTGAGCGT
CAACGTGAAGATGGATCTGCGCCCGTTTCTCGCGATCAACCCGTGCGGCTATGCGGGGCTCGAAACGGTCGACATGGCGA
GCCTCGGCGCGACCGCCGACTGGCACGAAGTCGCGCGGACGCTCGTGCGCCGTCTGATCGCCAACCTCGACGGCGCAACC
GCGGCCGCCGCATTGCCGCAGCAGGCACTGGAACAATCGAATGACTGA

Upstream 100 bases:

>100_bases
AGCGTTCGTCGGCCGCCGCGCGGCCCGTTCGATCCGGCACCGGCGAGGTGCATGGGTTTTCGGTAAACTACCGTCCATGT
CAGCCTCGCCGGTTTCCATC

Downstream 100 bases:

>100_bases
CCTTACCGCTACCCCCGCACCCGCCGAACCGGCCGCGTCCGCCTACGATCCGACCGCCAAGCAGAAGGCGCAGGCGAAGA
CCGCGCGCATTCCGATCAAG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MPPASDAHAAPDAAASTSASPQSCAAPPDTLPVTVRWLGETPYDACFDAMRAFTDARTPDTGDEIWVVEHPPVYTLGQAG
NPAHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLIDLRRRKLMVRTLVTRIEQAVIETLAAYNLASVRKAGAPGIYVE
SGPHHGAKIAALGLKIRNGCSYHGLSVNVKMDLRPFLAINPCGYAGLETVDMASLGATADWHEVARTLVRRLIANLDGAT
AAAALPQQALEQSND

Sequences:

>Translated_255_residues
MPPASDAHAAPDAAASTSASPQSCAAPPDTLPVTVRWLGETPYDACFDAMRAFTDARTPDTGDEIWVVEHPPVYTLGQAG
NPAHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLIDLRRRKLMVRTLVTRIEQAVIETLAAYNLASVRKAGAPGIYVE
SGPHHGAKIAALGLKIRNGCSYHGLSVNVKMDLRPFLAINPCGYAGLETVDMASLGATADWHEVARTLVRRLIANLDGAT
AAAALPQQALEQSND
>Mature_254_residues
PPASDAHAAPDAAASTSASPQSCAAPPDTLPVTVRWLGETPYDACFDAMRAFTDARTPDTGDEIWVVEHPPVYTLGQAGN
PAHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLIDLRRRKLMVRTLVTRIEQAVIETLAAYNLASVRKAGAPGIYVES
GPHHGAKIAALGLKIRNGCSYHGLSVNVKMDLRPFLAINPCGYAGLETVDMASLGATADWHEVARTLVRRLIANLDGATA
AAALPQQALEQSND

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=206, Percent_Identity=49.5145631067961, Blast_Score=205, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323268, Length=194, Percent_Identity=28.3505154639175, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355483, Length=158, Percent_Identity=32.9113924050633, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_BURTA (Q2T1K6)

Other databases:

- EMBL:   CP000086
- RefSeq:   YP_440943.1
- ProteinModelPortal:   Q2T1K6
- SMR:   Q2T1K6
- STRING:   Q2T1K6
- GeneID:   3849380
- GenomeReviews:   CP000086_GR
- KEGG:   bte:BTH_I0385
- TIGR:   BTH_I0385
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- ProtClustDB:   PRK14343
- BioCyc:   BTHA271848:BTH_I0385-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 26977; Mature: 26846

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 202-202

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPASDAHAAPDAAASTSASPQSCAAPPDTLPVTVRWLGETPYDACFDAMRAFTDARTPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
TGDEIWVVEHPPVYTLGQAGNPAHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLIDLR
CCCEEEEEECCCEEEECCCCCCEEEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHH
RRKLMVRTLVTRIEQAVIETLAAYNLASVRKAGAPGIYVESGPHHGAKIAALGLKIRNGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEECCCC
SYHGLSVNVKMDLRPFLAINPCGYAGLETVDMASLGATADWHEVARTLVRRLIANLDGAT
CCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCH
AAAALPQQALEQSND
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
PPASDAHAAPDAAASTSASPQSCAAPPDTLPVTVRWLGETPYDACFDAMRAFTDARTPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
TGDEIWVVEHPPVYTLGQAGNPAHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLIDLR
CCCEEEEEECCCEEEECCCCCCEEEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHH
RRKLMVRTLVTRIEQAVIETLAAYNLASVRKAGAPGIYVESGPHHGAKIAALGLKIRNGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEECCCC
SYHGLSVNVKMDLRPFLAINPCGYAGLETVDMASLGATADWHEVARTLVRRLIANLDGAT
CCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCH
AAAALPQQALEQSND
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA