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The map label for this gene is ttcA [H]

Identifier: 83718777

GI number: 83718777

Start: 325365

End: 326360

Strand: Reverse

Name: ttcA [H]

Synonym: BTH_I0290

Alternate gene names: 83718777

Gene position: 326360-325365 (Counterclockwise)

Preceding gene: 83719649

Following gene: 83720136

Centisome position: 8.57

GC content: 65.36

Gene sequence:

>996_bases
ATGAATGCGCCCCACACCCCGCATCTGAACGAAGCCGACGCGGCCGCCGCCGTCGAGGCGAACGCGGCCGAGATCGGCCG
CCGCGCGCTCACGCGCCGCGAGCAGAAGGAAGCGTACGAGAACAACAAGCTGTTCAAGCGGCTCGTGCGCCAGGTCGGCC
AGGCGATCGGCGACTACAACATGATCGAGCACGGCGACAAGGTGATGGTCTGCCTGTCGGGCGGCAAGGACAGCTACGCG
CTGCTCGATATCCTGTTGCGGCTGCGCGAGCGCGCGCCGATCGATTTCGACCTCGTCGCCGTGAACCTCGACCAGAAGCA
GCCGGGCTTTCCGGAGCACGTGCTGCCCGAGTACCTGACGAAGATCGGCGTGCCGTTTCACATCGAGAACCAGGATACGT
ACAGCATCGTCAAGCGGCTCGTGCCCGAAGGCAAGACCACCTGCTCGCTGTGCTCGCGGCTGCGCCGCGGGATTCTGTAC
CGCGTCGCGGGCGAACTCGGCGCGACGAAGATCGCGCTCGGCCACCACCGCGACGACATCGTGCAGACGCTGCTGCTGAA
CATGTTCTACGGCGGCAAGCTGAAGGGGATGCCGCCGAAGCTGCAATCGGATGACGGCAAGAACATCGTGATCCGCCCGC
TCGCCTACGCGAAGGAAACCGATCTCGAGAAATACGCGGAACTGCGCGAATTCCCGATCATTCCGTGCAATCTGTGCGGC
AGCCAGCCGAACCTGAAGCGCGCGGAGATGAAGGCGCTGATCCGCGACTGGGACAAGCGCTTTCCGGGCCGCGTCGACAA
CATGTTCAACGCGCTCGCGAACGTCGTGCCGTCGCACCTGATGGACACGCAGTTGTTCCCGTTCGCCGGACTGCGCGCGA
CGGGCGAGGCCGATCCGAACGGCGACATCGCGTTCGACGAGGACCCGTGCGGCACCGGCGCGAGCGCGCCGGGCGGCGCG
AAATCCGTGTCGATCGTGCAGTTCGACGATCTGTAA

Upstream 100 bases:

>100_bases
CGGTGCGCGCGGTGCGCGTCTCCACCGAAAAGCCGGACGCCTATCCCGATTGCGACGCCGTCGGCGTCGAAGTATTTCGC
ATCAAGGACGAGGAGCGAGC

Downstream 100 bases:

>100_bases
CCCGCCATTCGCGGCGCGCGCCGCGAAACGAAACGAACGGCGGCGCGCCGGCCTGCCGCGCGCCGCCGGAACCCGAGCCC
GCGCCGATCCCGCGGCCGCG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNAPHTPHLNEADAAAAVEANAAEIGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYNMIEHGDKVMVCLSGGKDSYA
LLDILLRLRERAPIDFDLVAVNLDQKQPGFPEHVLPEYLTKIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILY
RVAGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDTQLFPFAGLRATGEADPNGDIAFDEDPCGTGASAPGGA
KSVSIVQFDDL

Sequences:

>Translated_331_residues
MNAPHTPHLNEADAAAAVEANAAEIGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYNMIEHGDKVMVCLSGGKDSYA
LLDILLRLRERAPIDFDLVAVNLDQKQPGFPEHVLPEYLTKIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILY
RVAGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDTQLFPFAGLRATGEADPNGDIAFDEDPCGTGASAPGGA
KSVSIVQFDDL
>Mature_331_residues
MNAPHTPHLNEADAAAAVEANAAEIGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYNMIEHGDKVMVCLSGGKDSYA
LLDILLRLRERAPIDFDLVAVNLDQKQPGFPEHVLPEYLTKIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILY
RVAGELGATKIALGHHRDDIVQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDTQLFPFAGLRATGEADPNGDIAFDEDPCGTGASAPGGA
KSVSIVQFDDL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=274, Percent_Identity=68.978102189781, Blast_Score=410, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI193211090, Length=285, Percent_Identity=30.8771929824561, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=237, Percent_Identity=29.535864978903, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24651972, Length=246, Percent_Identity=27.6422764227642, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 36649; Mature: 36649

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPHTPHLNEADAAAAVEANAAEIGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYN
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MIEHGDKVMVCLSGGKDSYALLDILLRLRERAPIDFDLVAVNLDQKQPGFPEHVLPEYLT
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
KIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDI
HCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
VQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHCCHHHHHHHHHCCCCCHHCCC
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDTQLFPFAGLRATGEADPN
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GDIAFDEDPCGTGASAPGGAKSVSIVQFDDL
CCEEECCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNAPHTPHLNEADAAAAVEANAAEIGRRALTRREQKEAYENNKLFKRLVRQVGQAIGDYN
CCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MIEHGDKVMVCLSGGKDSYALLDILLRLRERAPIDFDLVAVNLDQKQPGFPEHVLPEYLT
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
KIGVPFHIENQDTYSIVKRLVPEGKTTCSLCSRLRRGILYRVAGELGATKIALGHHRDDI
HCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHH
VQTLLLNMFYGGKLKGMPPKLQSDDGKNIVIRPLAYAKETDLEKYAELREFPIIPCNLCG
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHCCHHHHHHHHHCCCCCHHCCC
SQPNLKRAEMKALIRDWDKRFPGRVDNMFNALANVVPSHLMDTQLFPFAGLRATGEADPN
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GDIAFDEDPCGTGASAPGGAKSVSIVQFDDL
CCEEECCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA