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The map label for this gene is argT [H]

Identifier: 83718624

GI number: 83718624

Start: 1016058

End: 1017149

Strand: Reverse

Name: argT [H]

Synonym: BTH_I0890

Alternate gene names: 83718624

Gene position: 1017149-1016058 (Counterclockwise)

Preceding gene: 83720654

Following gene: 83720821

Centisome position: 26.7

GC content: 67.49

Gene sequence:

>1092_bases
ATGCACCGGTTCGTTGCGCCGATCCATGCAATCGTCCGCGCGATTCGCGCGCGCTGCCGCGCCGGCGAGCAAAGCGAGGC
CGACGCGCCCGCCCGGCGCGCGTCGCGCTCGCCCGCATCGTTTCTGCTGGGCCGCGGCCGGCGTTCGGGTAAAATACCGC
GCTTTTGGCCGCCGTCGACGGCGCTAGGCCCCCAGGCCGAGCCGAGCCGAGCCGATATCGACGAACGCGAATTTTGGCCG
CCTAGAATACGCCCGGCGCGCGCATGCCCGGACGCATCCGGCCGCGCGGCCGGGGAAGTCAGGAAGAGGAACGACATGAA
GAAGCTCGCCTTGTGCGCAGCCCTCGCCCTTGCGGCGGGCGGCGCCTTCGCGAAGGAATGGAAGACCGTGCGGATCGGCG
TCGACGCCAGCTATCCGCCGTTCGAATCGACCGCGCCGAGCGGCGAGATCGTCGGATTCGACGTCGATCTCGCGAACGAG
GTCTGCAAGCGGATCAACGCGAAGTGCACATGGACGCCGCAGGATCTCGACGGCATCATCCCCGCGCTGAAGGCGAAGAA
ATTCGACGTGATCGTGTCGTCGCTGACCGTCACCGACAAGCGCCGCGAGCAGATCGACTTCTCCGACAAGCTGTACGACG
CGCCCGCGCGGATGATCGCGAAGGCGGGCTCGCCGCTCCTGCCGAGCGTCGAGTCGCTGAAAGGCAAGCGCGTCGGCGTC
GAGCAGGGCTCGACCCAGGAGACTTTCGCGAAGGCGCACTGGGAGCCGAAGGGCGTGACGATCGTGTCGTACCAGAACCA
GGATCAGGTGTACGCCGATCTCGGCTCGGGCCGTCTCGACGCGACGCTGCAAGACGAGTTCCAGGCCGACTACGGCTTCC
TGCGCACGCCGCGCGGCAAGGGTTTCGCATGGGCGGGCCCGGCCGTGAAGGACCCGAAGACGCTCGGCGACGGCACCGCG
ATGGGCTTGCGCAAGGACGACGCGGACCTGAAGAACGCGATCAACCGCGCGCTCGCGTCGATGCACAAGGACGGCACGTA
CGACAGGCTGTCGCGCAAGTACTTCCCGTACAGCGTCTATTCGGCGAAGTAA

Upstream 100 bases:

>100_bases
GATCGCCCGCGCGGCGGATCTCGAACATGACGCCTCCCACTGAGAAATATCGACAGCCTCGAATGTAGAGGTCGCGTGCA
CCTGCAACAATCCGGCGTGA

Downstream 100 bases:

>100_bases
GCGCCGAGCGGGCCGCGCCGAGTGACCGGCGCGCGGCCCGCGCAGCACCAGCGGTCGACACCACGCGCCGCAATGCGCCG
CCGCCCTGCCCGATCGCAGG

Product: amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MHRFVAPIHAIVRAIRARCRAGEQSEADAPARRASRSPASFLLGRGRRSGKIPRFWPPSTALGPQAEPSRADIDEREFWP
PRIRPARACPDASGRAAGEVRKRNDMKKLALCAALALAAGGAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLANE
VCKRINAKCTWTPQDLDGIIPALKAKKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSPLLPSVESLKGKRVGV
EQGSTQETFAKAHWEPKGVTIVSYQNQDQVYADLGSGRLDATLQDEFQADYGFLRTPRGKGFAWAGPAVKDPKTLGDGTA
MGLRKDDADLKNAINRALASMHKDGTYDRLSRKYFPYSVYSAK

Sequences:

>Translated_363_residues
MHRFVAPIHAIVRAIRARCRAGEQSEADAPARRASRSPASFLLGRGRRSGKIPRFWPPSTALGPQAEPSRADIDEREFWP
PRIRPARACPDASGRAAGEVRKRNDMKKLALCAALALAAGGAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLANE
VCKRINAKCTWTPQDLDGIIPALKAKKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSPLLPSVESLKGKRVGV
EQGSTQETFAKAHWEPKGVTIVSYQNQDQVYADLGSGRLDATLQDEFQADYGFLRTPRGKGFAWAGPAVKDPKTLGDGTA
MGLRKDDADLKNAINRALASMHKDGTYDRLSRKYFPYSVYSAK
>Mature_363_residues
MHRFVAPIHAIVRAIRARCRAGEQSEADAPARRASRSPASFLLGRGRRSGKIPRFWPPSTALGPQAEPSRADIDEREFWP
PRIRPARACPDASGRAAGEVRKRNDMKKLALCAALALAAGGAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLANE
VCKRINAKCTWTPQDLDGIIPALKAKKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSPLLPSVESLKGKRVGV
EQGSTQETFAKAHWEPKGVTIVSYQNQDQVYADLGSGRLDATLQDEFQADYGFLRTPRGKGFAWAGPAVKDPKTLGDGTA
MGLRKDDADLKNAINRALASMHKDGTYDRLSRKYFPYSVYSAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=56.5891472868217, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=56.2231759656652, Blast_Score=293, Evalue=8e-81,
Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=38.4920634920635, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787088, Length=254, Percent_Identity=36.2204724409449, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788228, Length=237, Percent_Identity=31.2236286919831, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=31.5573770491803, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 39734; Mature: 39734

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRFVAPIHAIVRAIRARCRAGEQSEADAPARRASRSPASFLLGRGRRSGKIPRFWPPST
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCC
ALGPQAEPSRADIDEREFWPPRIRPARACPDASGRAAGEVRKRNDMKKLALCAALALAAG
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLANEVCKRINAKCTWTPQDLDGII
CHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEECHHHHHHHH
PALKAKKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSPLLPSVESLKGKRVGV
HHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEECC
EQGSTQETFAKAHWEPKGVTIVSYQNQDQVYADLGSGRLDATLQDEFQADYGFLRTPRGK
CCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHCCCCEEECCCCC
GFAWAGPAVKDPKTLGDGTAMGLRKDDADLKNAINRALASMHKDGTYDRLSRKYFPYSVY
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEE
SAK
CCC
>Mature Secondary Structure
MHRFVAPIHAIVRAIRARCRAGEQSEADAPARRASRSPASFLLGRGRRSGKIPRFWPPST
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCC
ALGPQAEPSRADIDEREFWPPRIRPARACPDASGRAAGEVRKRNDMKKLALCAALALAAG
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GAFAKEWKTVRIGVDASYPPFESTAPSGEIVGFDVDLANEVCKRINAKCTWTPQDLDGII
CHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEECHHHHHHHH
PALKAKKFDVIVSSLTVTDKRREQIDFSDKLYDAPARMIAKAGSPLLPSVESLKGKRVGV
HHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEECC
EQGSTQETFAKAHWEPKGVTIVSYQNQDQVYADLGSGRLDATLQDEFQADYGFLRTPRGK
CCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHCCCCEEECCCCC
GFAWAGPAVKDPKTLGDGTAMGLRKDDADLKNAINRALASMHKDGTYDRLSRKYFPYSVY
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEE
SAK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]