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The map label for this gene is livK [C]

Identifier: 83718587

GI number: 83718587

Start: 3600701

End: 3601843

Strand: Reverse

Name: livK [C]

Synonym: BTH_I3158

Alternate gene names: 83718587

Gene position: 3601843-3600701 (Counterclockwise)

Preceding gene: 83721023

Following gene: 83720339

Centisome position: 94.56

GC content: 63.52

Gene sequence:

>1143_bases
ATGAATATCAAGATGCAAAAGCTGCTGCCGATCAGCGCGGCGGCTATGCTGTTGGCTGCAGCCGCCACGAACGCTGCCGC
CGACCAGGTCGTCAAGATCGGCCATGTCGCACCGTTGACGGGCGGGATCGCCCACCTTGGTAAGGACAACGAAAATGGTG
CGCGCCTTGCGGTCGAGGAGATCAATGCGAAGGGTCTCACGATCGGCGGCCAGAAGATTACGCTCCAGCTCGACGCCCAG
GACGACGCGGCTGACCCGCGCACCGCGACGCAAGTCGCGCAGAAGCTCGTCGACGACAAGGTTGTCGCGGTCGTCGGTCA
CCTGAACTCGGGCACGTCGATCCCGGCCTCGAAGATCTACAGCGATGCGGGCATCGTGCAGATCTCGCCGTCGGCGACGA
ATCCGGCCTACACGCAGCAAGGCTTCAAGACGACCTACCGTGTCGTCGCGACCGACGCGCAGCAGGGCCCGGCGCTCGCG
AACTACGCGCGCTCGAAGGGCGTCAAGAGCGTCGCCGTCGTCGACGATTCGACCGCATACGGCCAGGGCCTTGCGGATGA
GTTCGAGAAGAAGGCGAAGGCGCTCGGCCTGAAGGTGCTGTCGCACGACGCGACGAACGACAAGGCCGTCGATTTCCGCG
CGATCCTGACGAAGATCAAGGGTGAAAATCCGGATGCCGTGATGTACGGCGGCATGGACGCCACCGGCGGCCCGTTCGCG
AAGCAGGCGAAGCAGCTCGGCCTGCGCGCGAAGATCTTCGCGGGCGACGGCGTCTGCACCGAAAAGCTCTCGGACCTCGC
GGGCGATGCGACCGACAACGTCGTGTGCTCGGAAGCGGGCGCGTCGCTCGAGAAGATGCCGGGCGGCGCGGCGTTCAAGG
CGAAGTACGAGAAGCGTTTCGGTCAGCCGATCCAGATCTACGCGCCGTTCACGTATGACGCGGTATATATCATCGTCGAC
GCGATGAAGCGCGCGAATTCGACGGACCCGGCAAAGATCGTCGCGGCGATGCCGAAGACGAATTACACGGGCGTGATCGG
CACGACGACCTTCGACTCGAAGGGCGACCTGCGGCACGGCGTGATCTCGCTGTACGATTACAAGGGCGGCAAGAAGACGT
TCCTGGATGAAGTGAAGATGTAA

Upstream 100 bases:

>100_bases
CAATGCAGCCGGCGAATGTCCAGTGATTTTGTGTCGAACACGAGGATCGGTGCGGGGCGTCGGTGAAAGCGTTTTCTTGG
GATTGAAACTGGAGACTTAC

Downstream 100 bases:

>100_bases
CCGTCAGCACGGCCGACGGAGGGGGCGGGGCGCGCATGCCCGAAGGCATGCGCGCCCCATTTTTTATCGGTGAAGAAGGC
GTGCGGCGCGCGCGGCGATC

Product: amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKITLQLDAQ
DDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYARSKGVKSVAVVDDSTAYGQGLADEFEKKAKALGLKVLSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLSDLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLRHGVISLYDYKGGKKTFLDEVKM

Sequences:

>Translated_380_residues
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKITLQLDAQ
DDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYARSKGVKSVAVVDDSTAYGQGLADEFEKKAKALGLKVLSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLSDLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLRHGVISLYDYKGGKKTFLDEVKM
>Mature_380_residues
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKITLQLDAQ
DDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYARSKGVKSVAVVDDSTAYGQGLADEFEKKAKALGLKVLSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLSDLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLRHGVISLYDYKGGKKTFLDEVKM

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=346, Percent_Identity=34.393063583815, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI48994941, Length=368, Percent_Identity=32.6086956521739, Blast_Score=177, Evalue=7e-46,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39834; Mature: 39834

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEE
CCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEEEEE
INAKGLTIGGQKITLQLDAQDDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIY
CCCCCEEECCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
SDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALANYARSKGVKSVAVVDDSTAY
CCCCEEEECCCCCCCHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCHH
GQGLADEFEKKAKALGLKVLSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
CCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHH
KQAKQLGLRAKIFAGDGVCTEKLSDLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRF
HHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHC
GQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLRHG
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHEEEECCCCCCCEEEEECCCCCCCCCCCC
VISLYDYKGGKKTFLDEVKM
EEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEE
CCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEEEEE
INAKGLTIGGQKITLQLDAQDDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIY
CCCCCEEECCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
SDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALANYARSKGVKSVAVVDDSTAY
CCCCEEEECCCCCCCHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCHH
GQGLADEFEKKAKALGLKVLSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
CCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHH
KQAKQLGLRAKIFAGDGVCTEKLSDLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRF
HHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHC
GQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLRHG
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHEEEECCCCCCCEEEEECCCCCCCCCCCC
VISLYDYKGGKKTFLDEVKM
EEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA