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The map label for this gene is rpoN [H]

Identifier: 83718503

GI number: 83718503

Start: 538242

End: 539783

Strand: Reverse

Name: rpoN [H]

Synonym: BTH_I0486

Alternate gene names: 83718503

Gene position: 539783-538242 (Counterclockwise)

Preceding gene: 83720803

Following gene: 83721047

Centisome position: 14.17

GC content: 63.81

Gene sequence:

>1542_bases
GTGGCAAACTTCCGGTATGACTTCCCTTTTGCCATGAAAGCCAGTCTCCAACTTCGCCTGTCGCAGCATCTCGCGCTCAC
GCCCCAGCTTCAGCAATCGATCCGGCTGCTGCAGTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCAACGGCGATCGCGC
AGAACCCGCTGCTCGAGAACGACGACGACTGGATCGCGAGCCCCCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAG
ACGCCCGCGTCGTCCTCGCCCGAGCCGATGTCCGGCGCGTCGAACGGCACGACTGCGACGAATGAGCGCACCGAGCGCGA
CGAAACCGCCGGCGTCGACGAATACGACGGCTATTCGGCCGACGGCGGCGACGCGAGCCAGTGGAATCTCGACGACTTCG
GCCGCTCCCCCGCCGCATCCGACGACGACGATCTGCCGCCGATGCAGATCCACGAAACCTCGACGTCGCTGCGCGATCAT
CTGATGGCGCAGTTGCGCGTCACGCAGGCGAGCCCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGA
CGACGGCTACCTGAGCGCGTCGCTCGACGAAGTGCTCGCCGATCTGCCGGCGGAGCTCGAAGTCGACGTCGACGAACTGA
ACGCGGCGCTCGCGCTCGTGCACAGCTTCGATCCGGCGGGCGTCGGCGCGCGGTCCGCGTCCGAATGCCTGAAGCTGCAG
TTGCTGCGGCTGGATCCGTCCCCGACGCGCACGCTCGCGCTCGATATCGTGTCGCAGCATCTCGAACTGCTTGCCGCGCG
CGATTTCACGCGCCTGCGCAAGCAACTGAAGGCGACCGACGACGAGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCG
AGCCGTTTCCCGGCGCGGCGTACGGCAAGGCCGAAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAG
AGCTGGCAGGCGGAACTCAATCCGGAAGTGGTGCCGAAGCTGCGTATCAACCATCTTTACGCGAACATCCTGCGCAACAG
CCGGGGCGATCCGAGCGCGGGATCGCTGAAGCAGCAGCTTCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGGT
TCGAGACGATCCTGCGGGTCGCGCAGGCGATTGTCGAACGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGC
CCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACAT
GCTGACTCCGTTCGGCACACTTGAATTTAAGTACTTCTTCGGGTCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGT
CGACAGCGATCCGCGCCCTCATCAAACAACTGATAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCC
GAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCGCGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGT
GAATCTGCGCAAGTCTCTGTAG

Upstream 100 bases:

>100_bases
CCGGCGCGATCCGCGCCGCCTCACCGCACGCCCGGTTCGCGCCGCGCGTGCGCGCCCCGCGCGCCGAGCGTCATCCCGAA
TGCCTCAACGCGCCGCCCTC

Downstream 100 bases:

>100_bases
CCCGTCGCCACGCGGCGGGCGTTTTCCGGCCGCCGCATCGTCGACGGCAAAGCCGGCCGACCGATGCCCGCTCACCGCGA
GCATCGGCAACGAAATCGGC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MANFRYDFPFAMKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQ
TPASSSPEPMSGASNGTTATNERTERDETAGVDEYDGYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDH
LMAQLRVTQASPRDRALVTFLIESLDDDGYLSASLDEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQ
LLRLDPSPTRTLALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQ
SWQAELNPEVVPKLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMR
PLVLREIADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIA
ELLAEQGFVVARRTVAKYREALKIPAVNLRKSL

Sequences:

>Translated_513_residues
MANFRYDFPFAMKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQ
TPASSSPEPMSGASNGTTATNERTERDETAGVDEYDGYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDH
LMAQLRVTQASPRDRALVTFLIESLDDDGYLSASLDEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQ
LLRLDPSPTRTLALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQ
SWQAELNPEVVPKLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMR
PLVLREIADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIA
ELLAEQGFVVARRTVAKYREALKIPAVNLRKSL
>Mature_512_residues
ANFRYDFPFAMKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQT
PASSSPEPMSGASNGTTATNERTERDETAGVDEYDGYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHL
MAQLRVTQASPRDRALVTFLIESLDDDGYLSASLDEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQL
LRLDPSPTRTLALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQS
WQAELNPEVVPKLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRP
LVLREIADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAE
LLAEQGFVVARRTVAKYREALKIPAVNLRKSL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=507, Percent_Identity=44.1814595660749, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56342; Mature: 56211

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFRYDFPFAMKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLEN
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DDDWIASPLRVAADGSVIAQTPASSSPEPMSGASNGTTATNERTERDETAGVDEYDGYSA
CCCHHCCCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
DGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQASPRDRALVTF
CCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LIESLDDDGYLSASLDEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQ
HHHHCCCCCCCHHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEE
LLRLDPSPTRTLALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAA
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
YGKAEADYVVPDIIVKKAGQSWQAELNPEVVPKLRINHLYANILRNSRGDPSAGSLKQQL
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
QEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHESTVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
VTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIA
HCCCCEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ELLAEQGFVVARRTVAKYREALKIPAVNLRKSL
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure 
ANFRYDFPFAMKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLEN
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DDDWIASPLRVAADGSVIAQTPASSSPEPMSGASNGTTATNERTERDETAGVDEYDGYSA
CCCHHCCCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
DGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQASPRDRALVTF
CCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LIESLDDDGYLSASLDEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQ
HHHHCCCCCCCHHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEE
LLRLDPSPTRTLALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAA
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
YGKAEADYVVPDIIVKKAGQSWQAELNPEVVPKLRINHLYANILRNSRGDPSAGSLKQQL
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
QEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHESTVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
VTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIA
HCCCCEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ELLAEQGFVVARRTVAKYREALKIPAVNLRKSL
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]