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The map label for this gene is argT [H]

Identifier: 83718431

GI number: 83718431

Start: 3748950

End: 3749744

Strand: Reverse

Name: argT [H]

Synonym: BTH_I3294

Alternate gene names: 83718431

Gene position: 3749744-3748950 (Counterclockwise)

Preceding gene: 83719334

Following gene: 83721631

Centisome position: 98.44

GC content: 66.92

Gene sequence:

>795_bases
ATGAAACGACTGCAATTCCGGCTCGCGCTCGCCTGCGCATTGAGCGTCGCCGCGGGGGCCGGCGCGGCGCGCGCCGACGT
TTCGCAAACGCTGCGCTTCGGTCTCGAGGCGCAATATCCGCCGTTCGAATCGAAGGCGGCGAACGGCGAGCTGCAAGGCT
TCGATATCGACGTCGGCAACGCCGTCTGCAAGGAGGCGAAGCTCGCGTGCAAATGGGTCGAGACGTCGTTCGACGGACTG
ATCCCGGCGCTGCAGGGCCGCAAGTTCGACGCGATCAATTCGGCGATGAACGCGACCGACCAGCGCCGTCAGGCGATCGA
CTTCACGACGGTGATCTATCGCGTGCCGACGCGACTGATCGCGCGCGCGGACAGCGGCCTCGCGCCGACGCCGGAATCGT
TGAAGGGCAAGCGGGTGGGCGTGCTGCAGGGCTCGATTCAGGAGACGTATGCGAATGCGCACTGGGCGGGAGCGGGCGTG
CAGGTCGTCGCGTATCAGGACCAGAATCAGGCTTACTCGGATCTCACGGCGGGCCGCCTCGACGGCACGCTCGTGCTCGC
GCCCGCGGGCCAGCGCGGTTTCCTGTCGCGGCCGGACGCGAAGGGCTTCGCGTTCGTCGGGCAAGCGGTGCGCGACGACA
AGATCCTCGGAAGCGGGATCGCGTTCGGGCTGCGCAAGGGCGACGAAGCGCTGAAGCTGCGCCTGAACGCCGCGATCGAC
AAGCTGAAGGCGGACGGCACCGTGAAGGCGCTCGGCCGCAAGTATTTCGGCGATATCGACATCTCGACGAAGTGA

Upstream 100 bases:

>100_bases
ACGCGACGCTGATCGAAGGCGCGCGCCGTCTGCGCAGCCTCTGACTTGACGCGCCCGTCGCGCCGATCCGGACAAGACCC
GACCTCCAGGAGAATCATCG

Downstream 100 bases:

>100_bases
CGGAGGCGGCGCGCTGCGACGCCGCGCTTCGCGTGCGCTGGGCGCATGCGGCGTGCCCGTGCTTCGGGCATGTCGGCGAC
GGCAACGTGCACGTCGGCGT

Product: lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKRLQFRLALACALSVAAGAGAARADVSQTLRFGLEAQYPPFESKAANGELQGFDIDVGNAVCKEAKLACKWVETSFDGL
IPALQGRKFDAINSAMNATDQRRQAIDFTTVIYRVPTRLIARADSGLAPTPESLKGKRVGVLQGSIQETYANAHWAGAGV
QVVAYQDQNQAYSDLTAGRLDGTLVLAPAGQRGFLSRPDAKGFAFVGQAVRDDKILGSGIAFGLRKGDEALKLRLNAAID
KLKADGTVKALGRKYFGDIDISTK

Sequences:

>Translated_264_residues
MKRLQFRLALACALSVAAGAGAARADVSQTLRFGLEAQYPPFESKAANGELQGFDIDVGNAVCKEAKLACKWVETSFDGL
IPALQGRKFDAINSAMNATDQRRQAIDFTTVIYRVPTRLIARADSGLAPTPESLKGKRVGVLQGSIQETYANAHWAGAGV
QVVAYQDQNQAYSDLTAGRLDGTLVLAPAGQRGFLSRPDAKGFAFVGQAVRDDKILGSGIAFGLRKGDEALKLRLNAAID
KLKADGTVKALGRKYFGDIDISTK
>Mature_264_residues
MKRLQFRLALACALSVAAGAGAARADVSQTLRFGLEAQYPPFESKAANGELQGFDIDVGNAVCKEAKLACKWVETSFDGL
IPALQGRKFDAINSAMNATDQRRQAIDFTTVIYRVPTRLIARADSGLAPTPESLKGKRVGVLQGSIQETYANAHWAGAGV
QVVAYQDQNQAYSDLTAGRLDGTLVLAPAGQRGFLSRPDAKGFAFVGQAVRDDKILGSGIAFGLRKGDEALKLRLNAAID
KLKADGTVKALGRKYFGDIDISTK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=47.65625, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=46.8085106382979, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=34.6613545816733, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=33.0677290836653, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=32.6086956521739, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=28.968253968254, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28151; Mature: 28151

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLQFRLALACALSVAAGAGAARADVSQTLRFGLEAQYPPFESKAANGELQGFDIDVGN
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCH
AVCKEAKLACKWVETSFDGLIPALQGRKFDAINSAMNATDQRRQAIDFTTVIYRVPTRLI
HHHHHHHHHHHHHHHCHHHCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ARADSGLAPTPESLKGKRVGVLQGSIQETYANAHWAGAGVQVVAYQDQNQAYSDLTAGRL
HHCCCCCCCCCHHHCCCEEEEEECCHHHHHCCCEECCCCEEEEEEECCCCHHHHHHCCCC
DGTLVLAPAGQRGFLSRPDAKGFAFVGQAVRDDKILGSGIAFGLRKGDEALKLRLNAAID
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCHHHEEEHHHHHH
KLKADGTVKALGRKYFGDIDISTK
HHCCCCHHHHHHHHHCCCEECCCC
>Mature Secondary Structure
MKRLQFRLALACALSVAAGAGAARADVSQTLRFGLEAQYPPFESKAANGELQGFDIDVGN
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCH
AVCKEAKLACKWVETSFDGLIPALQGRKFDAINSAMNATDQRRQAIDFTTVIYRVPTRLI
HHHHHHHHHHHHHHHCHHHCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ARADSGLAPTPESLKGKRVGVLQGSIQETYANAHWAGAGVQVVAYQDQNQAYSDLTAGRL
HHCCCCCCCCCHHHCCCEEEEEECCHHHHHCCCEECCCCEEEEEEECCCCHHHHHHCCCC
DGTLVLAPAGQRGFLSRPDAKGFAFVGQAVRDDKILGSGIAFGLRKGDEALKLRLNAAID
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEECCCCCCHHHEEEHHHHHH
KLKADGTVKALGRKYFGDIDISTK
HHCCCCHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]