Click here to switch to the map view.

The map label for this gene is bioB [H]

Identifier: 83718411

GI number: 83718411

Start: 373545

End: 374564

Strand: Reverse

Name: bioB [H]

Synonym: BTH_I0337

Alternate gene names: 83718411

Gene position: 374564-373545 (Counterclockwise)

Preceding gene: 83721594

Following gene: 83720593

Centisome position: 9.83

GC content: 66.27

Gene sequence:

>1020_bases
ATGACCGACATGACTGAAGCTCAAACCGCTTGCCCGACGACCGAAACGCCGGTGGCCGCGCCCGTCGCGCCGCGCTGGCG
CGTCGCCGACGTGATCGCGCTCTACGAGCTGCCGTTCAACGATCTGCTGTTCCGCGCGCAGCAGACGCACCGCGAGCACT
TCGACGCGAACGCGATCCAGCTGTCGACGCTGCTATCGATCAAGACGGGCGGATGCGAGGAGGATTGCGGCTACTGCTCG
CAATCCGCGCATCACGACACCGGCCTGAAGGCGGAGAAACTGATGGAAGTGGACGCCGTGCTCGCCGCCGCGCGCACCGC
GAAGGAAAACGGCGCGACGCGCTTCTGCATGGGCGCCGCGTGGCGCAACCCGAAGGATCGCCACATCGAGCCGATCAAGG
AGATGATCCGCGGCGTGAAGGACATGGGCCTCGAGACGTGCGTGACGCTCGGCATGCTCGAAGAACACCAGGCGAAGGCG
CTGGCGGAGGCGGGCCTCGACTATTACAACCACAACCTCGACACGTCGCCCGAGTTCTACGGCCAGATCATCTCGACGCG
CACGTACCAGGATCGCCTCGACACGCTCGAGCGCGTGCGCGACGCGGGCATCAACGTGTGCTGCGGCGGGATCATCGGGA
TGGGCGAATCGCGCCGCGAGCGCGCAGGCCTCATCGCGCAGCTCGCGAACATGAACCCGTATCCGGAATCGGTGCCGATC
AACAATCTCGTCGCGATCGAGGGCACGCCGCTCGAGAACGCGCAAGCGCTCGATCCGTTCGAGTTCGTGCGCACGATCGC
GGTCGCGCGCATCACGATGCCGAAGGCAATGGTGCGCTTGTCGGCAGGCCGCGAGCAGCTCGACGATTCGATGCAGGCGC
TGTGCTTCCTCGCCGGCGCGAATTCGATGTTCTACGGCGACGTGCTGCTCACGACCGGCAATCCGCGCGCCGAAGCGGAT
CGCAAGCTGCTCGCGCGGCTCGGGATGTCGGCGACCGAAGCGACGCAGTTGAGCGCCTGA

Upstream 100 bases:

>100_bases
CTCGACGTGAACCGCTTGCTGGGCGCGCTGCGCGAAACCGCGCCGCGCTGACGCGCGCCCCGTTTATCACTGATTTTTCG
AATCGATCCCTGACCGAAGG

Downstream 100 bases:

>100_bases
ACGCCGGCGGCATGCCAGCAAAAAGCCGGGCGACGTCCCGGCTTTTTTGCGCGATGCGCAGCGCACGTCGCCCGCCGATG
CGCGCGCCGCACGCGCAACC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTDMTEAQTACPTTETPVAAPVAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQLSTLLSIKTGGCEEDCGYCS
QSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAAWRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKA
LAEAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPI
NNLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDSMQALCFLAGANSMFYGDVLLTTGNPRAEAD
RKLLARLGMSATEATQLSA

Sequences:

>Translated_339_residues
MTDMTEAQTACPTTETPVAAPVAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQLSTLLSIKTGGCEEDCGYCS
QSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAAWRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKA
LAEAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPI
NNLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDSMQALCFLAGANSMFYGDVLLTTGNPRAEAD
RKLLARLGMSATEATQLSA
>Mature_338_residues
TDMTEAQTACPTTETPVAAPVAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQLSTLLSIKTGGCEEDCGYCSQ
SAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAAWRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKAL
AEAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPIN
NLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDSMQALCFLAGANSMFYGDVLLTTGNPRAEADR
KLLARLGMSATEATQLSA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=316, Percent_Identity=61.3924050632911, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=50.1577287066246, Blast_Score=335, Evalue=7e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37114; Mature: 36982

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMTEAQTACPTTETPVAAPVAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQ
CCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEE
LSTLLSIKTGGCEEDCGYCSQSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAA
HHHHHEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
WRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKALAEAGLDYYNHNLDTSPEFY
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPI
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
NNLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDSMQALCFLAGA
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
NSMFYGDVLLTTGNPRAEADRKLLARLGMSATEATQLSA
CCEEECCEEEECCCCCHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
TDMTEAQTACPTTETPVAAPVAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQ
CCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEE
LSTLLSIKTGGCEEDCGYCSQSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAA
HHHHHEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
WRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKALAEAGLDYYNHNLDTSPEFY
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPI
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
NNLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDSMQALCFLAGA
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
NSMFYGDVLLTTGNPRAEADRKLLARLGMSATEATQLSA
CCEEECCEEEECCCCCHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA