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The map label for this gene is metG [H]

Identifier: 284159400

GI number: 284159400

Start: 972743

End: 975055

Strand: Reverse

Name: metG [H]

Synonym: BTH_I0856

Alternate gene names: 284159400

Gene position: 975055-972743 (Counterclockwise)

Preceding gene: 83720098

Following gene: 83719793

Centisome position: 25.6

GC content: 66.58

Gene sequence:

>2313_bases
GTGACATCCGCGCGAAGCGCCGCCGCCGTGCGCGAGACCGCCGCCCCGCGCGGCGAGTGCGGCCGCACGCGGCCCGCCCG
CACGTCGGCGGCCGGCCGCCCTGCTAGAATCGCAGCTTCCCTACGATTTTCCGCAAGCAGTCCCTTCAGAGACCCTATGT
CCGCATCCGACCTCCACCCCGTGCAGGCCGGCGCGCCGCAAGGCCGCCGCCAGATCCTCGTCACGTCCGCGCTGCCCTAT
GCGAACGGGCAGATTCACATCGGCCACCTGGTCGAGTACATCCAGACCGACATCTGGGCGCGGACGATGCGAATGCATGG
CCACGAGATCTACTACATCGGTGCGGACGACACGCACGGCACGCCCGTCATGCTGCGCGCCGAACAGGAAGGCGTGAGCC
CGAAGCAGTTGATCGAGCGCGTGTGGCGCGAGCACAAGCGCGATTTCGACAGCTTCGGCGTTTCGTTCGACAATTTCTAC
ACGACCGATTCGGACGAGAACCGCGTGCTCAGCGAGAAGATCTATCTCGCGTTGAAGGAGGCGGGGTTCATCGCCGAGCG
CGAGATCGAGCAGGCGTACGACCCCGTCAAGCAAATGTTCCTGCCGGATCGCTTCATCAAGGGCGAATGCCCGAAATGCC
ACGCGAAGGATCAGTACGGCGACAGTTGCGAGGTGTGCGGCACGACGTACCAGCCGACCGACCTCGTCAACCCGTACTCG
GTCGTCTCGGGTGCGACGCCCGTGCGCAAGACGTCGACGCACCACTTCTTCCGCCTGTCCGACCCGCGCTGCGAGGCGTT
CCTGCGCGAATGGGTGAGCGGCCTCGCGCAGCCCGAGGCGACCAACAAGATGCGCGAATGGCTCGGCGACGCGGGCGAGG
CGAAGCTCGCCGACTGGGACATCTCGCGCGACGCGCCGTACTTCGGCTTCGAGATTCCCGGCGCGCCGGGCAAGTACTTC
TACGTGTGGCTCGACGCGCCCGTCGGCTACTACGCGAGCTTCAAGAACCTGTGCGAGCGGCGCGGCCTCGATTTCGACGC
GTGGATCAGCAAGGATTCGACGACCGAGCAGTATCACTTCATCGGCAAGGACATCCTGTACTTCCACACGCTGTTCTGGC
CCGCGATGCTCGAGTTCTCGGGCCACCGCACGCCGACCAACGTGTTCGCGCACGGCTTCCTGACGGTCGACGGCGCGAAG
ATGTCGAAGTCGCGCGGCACGTTCATCACCGCGCAGAGCTACATCGACGCGGGCCTGAACCCCGAATGGCTGCGCTACTA
CTTCGCCGCGAAGCTGAACGCGACGATGGAAGACATCGACCTGAACCTCGAGGACTTCCAGGCGCGCGTCAATAGCGACC
TCGTCGGCAAGTACGTGAACATCGCGAGCCGCGCGGCCGGCTTCCTGATCAAGCGCTTCGACGGCCGCGTGCAGGCGAGC
GCGACGAATCATCCGCTTCTCGTGACGCTGCGCGACGCGATCCCGCAGATCGCCGCGCACTACGAGGCGCGCGAGTACGG
CCGCGCGCTGCGCCAGACGATGGAGCTCGCGGATGCGGTCAACGGCTATGTCGACACCGCAAAGCCGTGGGAGCTCGCGA
AGGACCCGGCGAACGCGGTCGCGCTGCACGAAACCTGCACTGTGAGCCTCGAGGCGTTCCGCCTGCTGTCGCTCGCACTC
AAGCCCGTGCTGCCGCGCGTCGCGGAAGGCGTCGAAGCGTTCCTCGGCATCGCGCCGCTCACGTGGGCCGACGCGAACAA
GCCGCTGTCGTCCGAGCAGCCGATCCGCGCGTATCAGCATCTGATGACCCGCGTCGATCCGAAGCAGATCGACGCGCTCC
TCGCGGCGAACCGCAGCTCGCTGCAAGGCGCCGCGGCGGCCGACGCGGCCGGCGCGACGAACGGCAACGGCGCGAAGGCC
GCGAAAAGCGCGAAAGCCGCGAATGCAGCGAGCGCCGACGACGAAGCATCGCCGTTCATCTCGATCGACGATTTCGCGAA
GATCGATCTGCGCATCGCGAAGATCGTCGCGTGCCAGGCGGTCGAAGGCTCGGACAAACTGCTGCAGCTCACGCTCGACG
TCGGCGAGGAGAAGACCCGCAACGTGTTCTCCGGCATCAAGTCCGCGTACCAGCCCGAGCAGCTCGTCGGCAAGCTGACC
GTGATGGTCGCGAACCTCGCGCCGCGCAAGATGAAGTTCGGCCTGTCCGAGGGGATGGTGCTCGCCGCGTCGGCAGCCGA
CGAGAAGGCCGAGCCGGGCCTCTACATCCTCGAGCCGCACAGCGGCGCGAAGCCCGGCATGCGCGTGAAGTAA

Upstream 100 bases:

>100_bases
CAACATCCCTTCCGTCTCTCCGGCGCTGTTCGGCACCGCATTGCCGGCATCAATCTAGCCGCCCCGTCCAAATCTTTCAA
TTCGCAAGTTATTACGCTTC

Downstream 100 bases:

>100_bases
GCGCGCATCGCCGCGACGCTTCCGCAAGGGCCGCGCAAGCGGCCCTTCGCGCTTTGCGCGAGTGCCGGCGCCATCGCATC
GCGCGAGGTCACGACATCAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MTSARSAAAVRETAAPRGECGRTRPARTSAAGRPARIAASLRFSASSPFRDPMSASDLHPVQAGAPQGRRQILVTSALPY
ANGQIHIGHLVEYIQTDIWARTMRMHGHEIYYIGADDTHGTPVMLRAEQEGVSPKQLIERVWREHKRDFDSFGVSFDNFY
TTDSDENRVLSEKIYLALKEAGFIAEREIEQAYDPVKQMFLPDRFIKGECPKCHAKDQYGDSCEVCGTTYQPTDLVNPYS
VVSGATPVRKTSTHHFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGDAGEAKLADWDISRDAPYFGFEIPGAPGKYF
YVWLDAPVGYYASFKNLCERRGLDFDAWISKDSTTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAK
MSKSRGTFITAQSYIDAGLNPEWLRYYFAAKLNATMEDIDLNLEDFQARVNSDLVGKYVNIASRAAGFLIKRFDGRVQAS
ATNHPLLVTLRDAIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWELAKDPANAVALHETCTVSLEAFRLLSLAL
KPVLPRVAEGVEAFLGIAPLTWADANKPLSSEQPIRAYQHLMTRVDPKQIDALLAANRSSLQGAAAADAAGATNGNGAKA
AKSAKAANAASADDEASPFISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLT
VMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK

Sequences:

>Translated_770_residues
MTSARSAAAVRETAAPRGECGRTRPARTSAAGRPARIAASLRFSASSPFRDPMSASDLHPVQAGAPQGRRQILVTSALPY
ANGQIHIGHLVEYIQTDIWARTMRMHGHEIYYIGADDTHGTPVMLRAEQEGVSPKQLIERVWREHKRDFDSFGVSFDNFY
TTDSDENRVLSEKIYLALKEAGFIAEREIEQAYDPVKQMFLPDRFIKGECPKCHAKDQYGDSCEVCGTTYQPTDLVNPYS
VVSGATPVRKTSTHHFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGDAGEAKLADWDISRDAPYFGFEIPGAPGKYF
YVWLDAPVGYYASFKNLCERRGLDFDAWISKDSTTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAK
MSKSRGTFITAQSYIDAGLNPEWLRYYFAAKLNATMEDIDLNLEDFQARVNSDLVGKYVNIASRAAGFLIKRFDGRVQAS
ATNHPLLVTLRDAIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWELAKDPANAVALHETCTVSLEAFRLLSLAL
KPVLPRVAEGVEAFLGIAPLTWADANKPLSSEQPIRAYQHLMTRVDPKQIDALLAANRSSLQGAAAADAAGATNGNGAKA
AKSAKAANAASADDEASPFISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLT
VMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK
>Mature_769_residues
TSARSAAAVRETAAPRGECGRTRPARTSAAGRPARIAASLRFSASSPFRDPMSASDLHPVQAGAPQGRRQILVTSALPYA
NGQIHIGHLVEYIQTDIWARTMRMHGHEIYYIGADDTHGTPVMLRAEQEGVSPKQLIERVWREHKRDFDSFGVSFDNFYT
TDSDENRVLSEKIYLALKEAGFIAEREIEQAYDPVKQMFLPDRFIKGECPKCHAKDQYGDSCEVCGTTYQPTDLVNPYSV
VSGATPVRKTSTHHFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGDAGEAKLADWDISRDAPYFGFEIPGAPGKYFY
VWLDAPVGYYASFKNLCERRGLDFDAWISKDSTTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKM
SKSRGTFITAQSYIDAGLNPEWLRYYFAAKLNATMEDIDLNLEDFQARVNSDLVGKYVNIASRAAGFLIKRFDGRVQASA
TNHPLLVTLRDAIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWELAKDPANAVALHETCTVSLEAFRLLSLALK
PVLPRVAEGVEAFLGIAPLTWADANKPLSSEQPIRAYQHLMTRVDPKQIDALLAANRSSLQGAAAADAAGATNGNGAKAA
KSAKAANAASADDEASPFISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLTV
MVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=526, Percent_Identity=27.5665399239544, Blast_Score=208, Evalue=2e-53,
Organism=Homo sapiens, GI24308436, Length=538, Percent_Identity=26.5799256505576, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI4507947, Length=162, Percent_Identity=30.2469135802469, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788432, Length=708, Percent_Identity=53.1073446327684, Blast_Score=756, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=677, Percent_Identity=25.8493353028065, Blast_Score=229, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=542, Percent_Identity=22.6937269372694, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992781, Length=283, Percent_Identity=25.0883392226148, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321704, Length=566, Percent_Identity=28.2685512367491, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=23.8532110091743, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24655311, Length=540, Percent_Identity=28.8888888888889, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI21358483, Length=520, Percent_Identity=26.9230769230769, Blast_Score=138, Evalue=2e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 84798; Mature: 84666

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSARSAAAVRETAAPRGECGRTRPARTSAAGRPARIAASLRFSASSPFRDPMSASDLHP
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCC
VQAGAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWARTMRMHGHEIYYIGADDTHG
CCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
TPVMLRAEQEGVSPKQLIERVWREHKRDFDSFGVSFDNFYTTDSDENRVLSEKIYLALKE
CCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AGFIAEREIEQAYDPVKQMFLPDRFIKGECPKCHAKDQYGDSCEVCGTTYQPTDLVNPYS
CCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHH
VVSGATPVRKTSTHHFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGDAGEAKLADWD
HHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCC
ISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCERRGLDFDAWISKDSTTEQYHF
CCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHH
IGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDAGLN
HHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEHHHHCCCCC
PEWLRYYFAAKLNATMEDIDLNLEDFQARVNSDLVGKYVNIASRAAGFLIKRFDGRVQAS
HHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
ATNHPLLVTLRDAIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWELAKDPANAV
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEE
ALHETCTVSLEAFRLLSLALKPVLPRVAEGVEAFLGIAPLTWADANKPLSSEQPIRAYQH
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHH
LMTRVDPKQIDALLAANRSSLQGAAAADAAGATNGNGAKAAKSAKAANAASADDEASPFI
HHHCCCHHHHHHHHHCCCHHCCCCHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEE
SIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLT
ECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
VMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK
HHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TSARSAAAVRETAAPRGECGRTRPARTSAAGRPARIAASLRFSASSPFRDPMSASDLHP
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCC
VQAGAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWARTMRMHGHEIYYIGADDTHG
CCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
TPVMLRAEQEGVSPKQLIERVWREHKRDFDSFGVSFDNFYTTDSDENRVLSEKIYLALKE
CCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AGFIAEREIEQAYDPVKQMFLPDRFIKGECPKCHAKDQYGDSCEVCGTTYQPTDLVNPYS
CCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHH
VVSGATPVRKTSTHHFFRLSDPRCEAFLREWVSGLAQPEATNKMREWLGDAGEAKLADWD
HHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCC
ISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCERRGLDFDAWISKDSTTEQYHF
CCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHH
IGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDAGLN
HHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEHHHHCCCCC
PEWLRYYFAAKLNATMEDIDLNLEDFQARVNSDLVGKYVNIASRAAGFLIKRFDGRVQAS
HHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
ATNHPLLVTLRDAIPQIAAHYEAREYGRALRQTMELADAVNGYVDTAKPWELAKDPANAV
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEE
ALHETCTVSLEAFRLLSLALKPVLPRVAEGVEAFLGIAPLTWADANKPLSSEQPIRAYQH
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHH
LMTRVDPKQIDALLAANRSSLQGAAAADAAGATNGNGAKAAKSAKAANAASADDEASPFI
HHHCCCHHHHHHHHHCCCHHCCCCHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEE
SIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLT
ECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
VMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK
HHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA