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The map label for this gene is mnmE

Identifier: 83591335

GI number: 83591335

Start: 2625427

End: 2626815

Strand: Reverse

Name: mnmE

Synonym: Moth_2519

Alternate gene names: 83591335

Gene position: 2626815-2625427 (Counterclockwise)

Preceding gene: 83591336

Following gene: 83591334

Centisome position: 99.93

GC content: 55.08

Gene sequence:

>1389_bases
GTGCTTGACGATACCATTGCCGCCCTGGCGACACCGCCTGGTGAAGGCGGTATTAGCATAATTCGCCTGAGCGGCAGCCA
GGCCATTGCTATAGTAGCAAAGGTTTTTAAACCGGTCAAGGGACCTGATTTAACCACAACCAGGAGTCATACCCTGCGGT
TGGGATTCATAATTGATCCTGTTTCTGGGGAAAGTCTGGACGAGGTTCTGGTTAGTGTCATGCGGGCTCCTCATAGTTAT
ACGGCTGAGGATGTGGTGGAGATAAACTGCCACGGGGGCGCCCTGGCCACGTCCAGGGTGCTGCAACTGGTCCTGAGAAC
CGGTGCCAGGTTGGCCGAGCCGGGAGAGTTCACTCGCCGGGCTTTCCTCAACGGCCGCCTGGACCTGGCCCAGGCCGAAG
CGGTCCTGGAGATTATCCGCGCCAGGAGCAGCAGGGGTTTGACGGCAGCCCTGGATCACCTACGGGGTAACCTCTCCCGG
AAGATTGGCGAACTGAATGAACGCTTGACCGGCATCCTGGCGGCCCTGGAAGCCAGTATGGATTTTCCTGAGGAGGTCGG
CGAGGTAGACCCGGAGAATCTAGCTGACCTGCGCCGCATCCTGGCGGGAGTTGACAGACTCCTGGCTACCTGGGAAGAAG
GCCGACTTTTAACTGAAGGCTTAAAAGTAGCTATTGTCGGCCGACCCAATGTCGGCAAATCAAGCCTGTTAAACGCCCTG
TTAAACCAGGAACGAGCCATTGTCAGCAACATCCCAGGTACCACCAGGGATACCATTGAGGAAACCCTGCAACTCGGGGG
ATTTACCTGCCGCTTGATAGATACAGCCGGGCTGCGGGAGACAGCGGATGAATTGGAGAGCATCGGCGTAGCCAGAAGTA
AGAAGGCCATTGCAGCGGCTGACCTGGTGCTGGTGGTTGTTGACCTGCAAACAGGAATCCAGGATGAAGACCGGCGCGTT
TTGGAGAGTGTCAGGGATAAGGTTTTGATAATCATAGGCAACAAGCTGGATCTTGTAGCCCACGATATAAATAAAAAATT
GGCTGACCTCGAATCCTTTGCCGGAAATTATCCCCGGGTAGCTGTTTCCGCCCTCAAAGGTAAAGGATTAGATGAACTGG
CCAGAAAAGTCCAGGAGATTGTCCTGGGTGGAAGAGCCCTGGCAGGTAGCGATGAACCCTTAATCACCAATGCCCGTCAC
CGGGCTGCCCTGGAAAATTGCCGGGAGCACCTGGCCAGCGCCATTAAAGCCTGGGAAGAAGGATTACCTGAGGATTTAAT
CGCCATTGACCTCTGGTCAGCAGCAGATTACCTGGGAGAAATCATCGGAACCACTGCCCGGGAGGATCTTCTGGACCGGA
TATTCAGCGATTTCTGCATCGGCAAGTAA

Upstream 100 bases:

>100_bases
GCGCCATTAACTATTTTGCGCTACAATAGTATTGCCCTGCAGGCCCAGTGATACCTTATGTATTACTGGGTCTTATTGTG
TAAAGAAGGTGCCGTAACCC

Downstream 100 bases:

>100_bases
ACAAGTAAAATAAAAAAGAAAGGATGGTGAAAGGGATGTACAACGCCGGTAATTATGATGTTATTGTTATCGGCGCCGGT
CACGCTGGTTGCGAAGCAGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MLDDTIAALATPPGEGGISIIRLSGSQAIAIVAKVFKPVKGPDLTTTRSHTLRLGFIIDPVSGESLDEVLVSVMRAPHSY
TAEDVVEINCHGGALATSRVLQLVLRTGARLAEPGEFTRRAFLNGRLDLAQAEAVLEIIRARSSRGLTAALDHLRGNLSR
KIGELNERLTGILAALEASMDFPEEVGEVDPENLADLRRILAGVDRLLATWEEGRLLTEGLKVAIVGRPNVGKSSLLNAL
LNQERAIVSNIPGTTRDTIEETLQLGGFTCRLIDTAGLRETADELESIGVARSKKAIAAADLVLVVVDLQTGIQDEDRRV
LESVRDKVLIIIGNKLDLVAHDINKKLADLESFAGNYPRVAVSALKGKGLDELARKVQEIVLGGRALAGSDEPLITNARH
RAALENCREHLASAIKAWEEGLPEDLIAIDLWSAADYLGEIIGTTAREDLLDRIFSDFCIGK

Sequences:

>Translated_462_residues
MLDDTIAALATPPGEGGISIIRLSGSQAIAIVAKVFKPVKGPDLTTTRSHTLRLGFIIDPVSGESLDEVLVSVMRAPHSY
TAEDVVEINCHGGALATSRVLQLVLRTGARLAEPGEFTRRAFLNGRLDLAQAEAVLEIIRARSSRGLTAALDHLRGNLSR
KIGELNERLTGILAALEASMDFPEEVGEVDPENLADLRRILAGVDRLLATWEEGRLLTEGLKVAIVGRPNVGKSSLLNAL
LNQERAIVSNIPGTTRDTIEETLQLGGFTCRLIDTAGLRETADELESIGVARSKKAIAAADLVLVVVDLQTGIQDEDRRV
LESVRDKVLIIIGNKLDLVAHDINKKLADLESFAGNYPRVAVSALKGKGLDELARKVQEIVLGGRALAGSDEPLITNARH
RAALENCREHLASAIKAWEEGLPEDLIAIDLWSAADYLGEIIGTTAREDLLDRIFSDFCIGK
>Mature_462_residues
MLDDTIAALATPPGEGGISIIRLSGSQAIAIVAKVFKPVKGPDLTTTRSHTLRLGFIIDPVSGESLDEVLVSVMRAPHSY
TAEDVVEINCHGGALATSRVLQLVLRTGARLAEPGEFTRRAFLNGRLDLAQAEAVLEIIRARSSRGLTAALDHLRGNLSR
KIGELNERLTGILAALEASMDFPEEVGEVDPENLADLRRILAGVDRLLATWEEGRLLTEGLKVAIVGRPNVGKSSLLNAL
LNQERAIVSNIPGTTRDTIEETLQLGGFTCRLIDTAGLRETADELESIGVARSKKAIAAADLVLVVVDLQTGIQDEDRRV
LESVRDKVLIIIGNKLDLVAHDINKKLADLESFAGNYPRVAVSALKGKGLDELARKVQEIVLGGRALAGSDEPLITNARH
RAALENCREHLASAIKAWEEGLPEDLIAIDLWSAADYLGEIIGTTAREDLLDRIFSDFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=474, Percent_Identity=35.2320675105485, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=35.2051835853132, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI193082988, Length=505, Percent_Identity=32.6732673267327, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI2367268, Length=472, Percent_Identity=37.2881355932203, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI87082120, Length=168, Percent_Identity=36.9047619047619, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17507259, Length=472, Percent_Identity=33.6864406779661, Blast_Score=205, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6323665, Length=502, Percent_Identity=30.6772908366534, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI45550813, Length=490, Percent_Identity=32.4489795918367, Blast_Score=199, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_MOOTA (Q2RFI8)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431344.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q2RFI8
- SMR:   Q2RFI8
- STRING:   Q2RFI8
- GeneID:   3832574
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2519
- NMPDR:   fig|264732.9.peg.2551
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q2RFI8
- BioCyc:   MTHE264732:MOTH_2519-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49757; Mature: 49757

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 87-87 BINDING 126-126 BINDING 462-462

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDTIAALATPPGEGGISIIRLSGSQAIAIVAKVFKPVKGPDLTTTRSHTLRLGFIIDP
CCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
VSGESLDEVLVSVMRAPHSYTAEDVVEINCHGGALATSRVLQLVLRTGARLAEPGEFTRR
CCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
AFLNGRLDLAQAEAVLEIIRARSSRGLTAALDHLRGNLSRKIGELNERLTGILAALEASM
HHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DFPEEVGEVDPENLADLRRILAGVDRLLATWEEGRLLTEGLKVAIVGRPNVGKSSLLNAL
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCHHHHHHHH
LNQERAIVSNIPGTTRDTIEETLQLGGFTCRLIDTAGLRETADELESIGVARSKKAIAAA
HHCCHHHHHCCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHH
DLVLVVVDLQTGIQDEDRRVLESVRDKVLIIIGNKLDLVAHDINKKLADLESFAGNYPRV
HHHHEEEEHHCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHH
AVSALKGKGLDELARKVQEIVLGGRALAGSDEPLITNARHRAALENCREHLASAIKAWEE
HHHHHHCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLPEDLIAIDLWSAADYLGEIIGTTAREDLLDRIFSDFCIGK
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDDTIAALATPPGEGGISIIRLSGSQAIAIVAKVFKPVKGPDLTTTRSHTLRLGFIIDP
CCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
VSGESLDEVLVSVMRAPHSYTAEDVVEINCHGGALATSRVLQLVLRTGARLAEPGEFTRR
CCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
AFLNGRLDLAQAEAVLEIIRARSSRGLTAALDHLRGNLSRKIGELNERLTGILAALEASM
HHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DFPEEVGEVDPENLADLRRILAGVDRLLATWEEGRLLTEGLKVAIVGRPNVGKSSLLNAL
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCHHHHHHHH
LNQERAIVSNIPGTTRDTIEETLQLGGFTCRLIDTAGLRETADELESIGVARSKKAIAAA
HHCCHHHHHCCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHH
DLVLVVVDLQTGIQDEDRRVLESVRDKVLIIIGNKLDLVAHDINKKLADLESFAGNYPRV
HHHHEEEEHHCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHH
AVSALKGKGLDELARKVQEIVLGGRALAGSDEPLITNARHRAALENCREHLASAIKAWEE
HHHHHHCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLPEDLIAIDLWSAADYLGEIIGTTAREDLLDRIFSDFCIGK
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA