Click here to switch to the map view.

The map label for this gene is ispD

Identifier: 83591303

GI number: 83591303

Start: 2592732

End: 2593421

Strand: Reverse

Name: ispD

Synonym: Moth_2487

Alternate gene names: 83591303

Gene position: 2593421-2592732 (Counterclockwise)

Preceding gene: 83591304

Following gene: 83591302

Centisome position: 98.65

GC content: 60.14

Gene sequence:

>690_bases
GTGCCTTTCCTGAGCATGATTATAGCCGCTGCCGGCCGTGGACGGCGCCTGGGCCGCGAAGTAAACAAAGTTTTTTTGCC
CCTGGCGGGTAAACCCATGCTGGCCTATAGCCTGGCCGTAGCTGTTGCTTCTCCCCTGGTGGATGAGATAATAATTGTTA
CCCGGCCGGATGACATTCCCCTCTGCCGACAGGTGGTGGCCGGGGAAGGGGCTGGCAAGGTAAAGGCTATAGTAGCCGGG
GGGGCCGAAAGACAGGATTCTATTGCTGAGGGTCTCAAAGCCGTGGCACGGGCAGCAGAATGGGTGGCCGTGCACGATGG
TGCGCGGCCTTTTTTAACACCGGCCTTACTAGAAAAAACAATTCTGGCCGCCCGGAACACTGGGGCAGCCATCGCCGCCC
TGCCGGTAAAAGAGACCATTAAACAGGGAGGTGAAGCCGGGATCGTGGCGGCGACCCTGGACCGGAAGGGTCTCTGGTCG
GCCCAGACTCCCCAGGTTTTCCGCCGGGAGTGGCTGGTAGACGCTTACCGTGAGGCAGCAGAAAAGGGTTGGCAGGCCAC
CGACGACGCCGCCCTGGTTGAAAGGTTGGGTTACCCGGTAAAGCTGGTACCCGGGGAGGAGACGAATATCAAGATTACCA
CCCCCGGCGACCTGGTCCTGGCCGGGGCCTTACTGGCAGGTGAAAAGTAA

Upstream 100 bases:

>100_bases
TTTTACAGACTGCCGCCGGGCGGATGATCTTTGCCCGGCCCAAGGCTGCTGACCGCAAACTTGGTGCCCATCACCAGGCC
CTGGAACGGAGCGAGTACCA

Downstream 100 bases:

>100_bases
TGCGCATTGGTTATGGCTATGACGTTCATGGCCTGGTACCGGGCCGGAAACTGGTCCTGGGGGGAGTAACCATACCTTAT
GACCGGGGCCTTGCAGGACA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPFLSMIIAAAGRGRRLGREVNKVFLPLAGKPMLAYSLAVAVASPLVDEIIIVTRPDDIPLCRQVVAGEGAGKVKAIVAG
GAERQDSIAEGLKAVARAAEWVAVHDGARPFLTPALLEKTILAARNTGAAIAALPVKETIKQGGEAGIVAATLDRKGLWS
AQTPQVFRREWLVDAYREAAEKGWQATDDAALVERLGYPVKLVPGEETNIKITTPGDLVLAGALLAGEK

Sequences:

>Translated_229_residues
MPFLSMIIAAAGRGRRLGREVNKVFLPLAGKPMLAYSLAVAVASPLVDEIIIVTRPDDIPLCRQVVAGEGAGKVKAIVAG
GAERQDSIAEGLKAVARAAEWVAVHDGARPFLTPALLEKTILAARNTGAAIAALPVKETIKQGGEAGIVAATLDRKGLWS
AQTPQVFRREWLVDAYREAAEKGWQATDDAALVERLGYPVKLVPGEETNIKITTPGDLVLAGALLAGEK
>Mature_228_residues
PFLSMIIAAAGRGRRLGREVNKVFLPLAGKPMLAYSLAVAVASPLVDEIIIVTRPDDIPLCRQVVAGEGAGKVKAIVAGG
AERQDSIAEGLKAVARAAEWVAVHDGARPFLTPALLEKTILAARNTGAAIAALPVKETIKQGGEAGIVAATLDRKGLWSA
QTPQVFRREWLVDAYREAAEKGWQATDDAALVERLGYPVKLVPGEETNIKITTPGDLVLAGALLAGEK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=230, Percent_Identity=25.6521739130435, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1789104, Length=218, Percent_Identity=38.9908256880734, Blast_Score=130, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_MOOTA (Q2RFM0)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431312.1
- ProteinModelPortal:   Q2RFM0
- SMR:   Q2RFM0
- STRING:   Q2RFM0
- GeneID:   3831590
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2487
- NMPDR:   fig|264732.9.peg.2518
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- PhylomeDB:   Q2RFM0
- BioCyc:   MTHE264732:MOTH_2487-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 24102; Mature: 23971

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFLSMIIAAAGRGRRLGREVNKVFLPLAGKPMLAYSLAVAVASPLVDEIIIVTRPDDIP
CCHHHHHHHHCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCH
LCRQVVAGEGAGKVKAIVAGGAERQDSIAEGLKAVARAAEWVAVHDGARPFLTPALLEKT
HHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
ILAARNTGAAIAALPVKETIKQGGEAGIVAATLDRKGLWSAQTPQVFRREWLVDAYREAA
HHHHCCCCCEEEECCHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKGWQATDDAALVERLGYPVKLVPGEETNIKITTPGDLVLAGALLAGEK
HCCCCCCCHHHHHHHCCCCEEEECCCCCCEEEECCCHHHHHHHHHCCCC
>Mature Secondary Structure 
PFLSMIIAAAGRGRRLGREVNKVFLPLAGKPMLAYSLAVAVASPLVDEIIIVTRPDDIP
CHHHHHHHHCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCH
LCRQVVAGEGAGKVKAIVAGGAERQDSIAEGLKAVARAAEWVAVHDGARPFLTPALLEKT
HHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
ILAARNTGAAIAALPVKETIKQGGEAGIVAATLDRKGLWSAQTPQVFRREWLVDAYREAA
HHHHCCCCCEEEECCHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKGWQATDDAALVERLGYPVKLVPGEETNIKITTPGDLVLAGALLAGEK
HCCCCCCCHHHHHHHCCCCEEEECCCCCCEEEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA