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The map label for this gene is ispF

Identifier: 83591302

GI number: 83591302

Start: 2592241

End: 2592732

Strand: Reverse

Name: ispF

Synonym: Moth_2486

Alternate gene names: 83591302

Gene position: 2592732-2592241 (Counterclockwise)

Preceding gene: 83591303

Following gene: 83591301

Centisome position: 98.63

GC content: 59.35

Gene sequence:

>492_bases
ATGCGCATTGGTTATGGCTATGACGTTCATGGCCTGGTACCGGGCCGGAAACTGGTCCTGGGGGGAGTAACCATACCTTA
TGACCGGGGCCTTGCAGGACACTCTGACGCTGATGTCCTACTCCACGCCTTGGCCGACGCCCTCCTGGGGGCAGCAGCGT
TGGGTGATATCGGTCATTACTTCCCCCCAGGGGATCCTCGCTATAAAGACGCCGATAGTATGCTCCTGCTGCGGGAAGTT
TACCGCCACGTCCAGACGGCCGGATACCGCCTGGCCAACGCCGATGCCACGATCACGGCCCAGGAGCCCCGGTTGGCCCC
TTATATTGATGCCATGCGGGAAAGAATTGCTGCAGCCCTGGGGGTCACAAAAGAGCAAATAAGTGTCAAGGCCACTACTA
CCGAGGGGCTGGGTTTTACCGGACGGGGCGAAGGTATCAGCGCCCAGGCGGTCGTATTGTTGCTTGACAGGGAAGCCGGA
GCCGGTTTATAA

Upstream 100 bases:

>100_bases
GTTACCCGGTAAAGCTGGTACCCGGGGAGGAGACGAATATCAAGATTACCACCCCCGGCGACCTGGTCCTGGCCGGGGCC
TTACTGGCAGGTGAAAAGTA

Downstream 100 bases:

>100_bases
TGACTCTGATTGAGATGAAAATAATAACTGCGATGAGCGGGAGAGTAAGCGTACCGTAAATCCCCAGAGAGGGGCCATCA
GCGGCTGGAAGTGGTCCTGG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MRIGYGYDVHGLVPGRKLVLGGVTIPYDRGLAGHSDADVLLHALADALLGAAALGDIGHYFPPGDPRYKDADSMLLLREV
YRHVQTAGYRLANADATITAQEPRLAPYIDAMRERIAAALGVTKEQISVKATTTEGLGFTGRGEGISAQAVVLLLDREAG
AGL

Sequences:

>Translated_163_residues
MRIGYGYDVHGLVPGRKLVLGGVTIPYDRGLAGHSDADVLLHALADALLGAAALGDIGHYFPPGDPRYKDADSMLLLREV
YRHVQTAGYRLANADATITAQEPRLAPYIDAMRERIAAALGVTKEQISVKATTTEGLGFTGRGEGISAQAVVLLLDREAG
AGL
>Mature_163_residues
MRIGYGYDVHGLVPGRKLVLGGVTIPYDRGLAGHSDADVLLHALADALLGAAALGDIGHYFPPGDPRYKDADSMLLLREV
YRHVQTAGYRLANADATITAQEPRLAPYIDAMRERIAAALGVTKEQISVKATTTEGLGFTGRGEGISAQAVVLLLDREAG
AGL

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=58.7096774193548, Blast_Score=175, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_MOOTA (Q2RFM1)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431311.1
- ProteinModelPortal:   Q2RFM1
- SMR:   Q2RFM1
- STRING:   Q2RFM1
- GeneID:   3831589
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2486
- NMPDR:   fig|264732.9.peg.2517
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q2RFM1
- BioCyc:   MTHE264732:MOTH_2486-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17210; Mature: 17210

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGYGYDVHGLVPGRKLVLGGVTIPYDRGLAGHSDADVLLHALADALLGAAALGDIGHY
CCCCCCCCEECCCCCCEEEECCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
FPPGDPRYKDADSMLLLREVYRHVQTAGYRLANADATITAQEPRLAPYIDAMRERIAAAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHH
GVTKEQISVKATTTEGLGFTGRGEGISAQAVVLLLDREAGAGL
CCCHHHEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MRIGYGYDVHGLVPGRKLVLGGVTIPYDRGLAGHSDADVLLHALADALLGAAALGDIGHY
CCCCCCCCEECCCCCCEEEECCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
FPPGDPRYKDADSMLLLREVYRHVQTAGYRLANADATITAQEPRLAPYIDAMRERIAAAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHH
GVTKEQISVKATTTEGLGFTGRGEGISAQAVVLLLDREAGAGL
CCCHHHEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA