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The map label for this gene is lutR [H]

Identifier: 83591125

GI number: 83591125

Start: 2421983

End: 2422684

Strand: Reverse

Name: lutR [H]

Synonym: Moth_2304

Alternate gene names: 83591125

Gene position: 2422684-2421983 (Counterclockwise)

Preceding gene: 83591126

Following gene: 83591124

Centisome position: 92.16

GC content: 58.97

Gene sequence:

>702_bases
GTGGACCTGAGACCTATCAGGACTAAAAAAATCTACGAAGAGATCGTCCAGCAGATTAAAGATCTCATTGGCGAGGGTAA
CCTGAAACCGGGGGACCGCCTGCCCTCGGAACGGGAATTGTCCGAACGCCTGGCCGTCAGTCGTGCTTCCGTCAGGGAAG
CCCTGAGTGCCCTGGCGGCCATGGGAGTAATTGTTATCAGGCCGGGAGAAGGCACATTTGTCCAGAATATCCGCAACGGG
GCCATAGTTGAACCCCTGGCCATGGCTCTGCTCCTGGACCGCCAGGCTGCTATGGAACTTCTGGAAGCCCGCCAGGCCCT
GGAGGGCGAGGCCGCATATCTTGCGGCCCGCCGCGCCGGGCCGGAGGACCTGGAAAAGATGGAGGAGCTTTTAAAGGAGA
TGGAGCATGACCTGCAGCGGGGCATTCTGGGTGAAGAGGCAGACCTGCGTTTCCACCTGGCCATTGCCGAGGCGGCCCGC
AACTCCGTCCTGGCGCGCCTGATGCATACAGTTTCCGATACCATGCGCCAGGCCCTGAAGACCAGCCGCCAGCGCCTTTA
TACCACCGCGGGCAACCCGGAAAAGCTTTTTGCCCAGCACAATCAGATTTATGAAGCCATCAAAGCCCATGACCCCCGGG
CCGCCAGGAAGGCTATCTGCCAGCACCTGCGCTTTGTCGAAAAGGAGTTGCAAAAAGAATAA

Upstream 100 bases:

>100_bases
AGGTCAAGCATACGGCTGAGGTGCTGGCGGCCTTGGAGAACTTGCAGGGTGGCGGGAACAGTAAATAACTCCCCGATTTT
ATTCCCGGAGGTGGGGACAG

Downstream 100 bases:

>100_bases
TGGGGTCAAAAATTAGCCAATCTGTTTTTATTGGTGATATTTAAGGTTTATAAAAATGAGGCCATGATTCAACTGTTGTA
TGGATTATAATTGACATAAA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDLRPIRTKKIYEEIVQQIKDLIGEGNLKPGDRLPSERELSERLAVSRASVREALSALAAMGVIVIRPGEGTFVQNIRNG
AIVEPLAMALLLDRQAAMELLEARQALEGEAAYLAARRAGPEDLEKMEELLKEMEHDLQRGILGEEADLRFHLAIAEAAR
NSVLARLMHTVSDTMRQALKTSRQRLYTTAGNPEKLFAQHNQIYEAIKAHDPRAARKAICQHLRFVEKELQKE

Sequences:

>Translated_233_residues
MDLRPIRTKKIYEEIVQQIKDLIGEGNLKPGDRLPSERELSERLAVSRASVREALSALAAMGVIVIRPGEGTFVQNIRNG
AIVEPLAMALLLDRQAAMELLEARQALEGEAAYLAARRAGPEDLEKMEELLKEMEHDLQRGILGEEADLRFHLAIAEAAR
NSVLARLMHTVSDTMRQALKTSRQRLYTTAGNPEKLFAQHNQIYEAIKAHDPRAARKAICQHLRFVEKELQKE
>Mature_233_residues
MDLRPIRTKKIYEEIVQQIKDLIGEGNLKPGDRLPSERELSERLAVSRASVREALSALAAMGVIVIRPGEGTFVQNIRNG
AIVEPLAMALLLDRQAAMELLEARQALEGEAAYLAARRAGPEDLEKMEELLKEMEHDLQRGILGEEADLRFHLAIAEAAR
NSVLARLMHTVSDTMRQALKTSRQRLYTTAGNPEKLFAQHNQIYEAIKAHDPRAARKAICQHLRFVEKELQKE

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=229, Percent_Identity=37.9912663755458, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786303, Length=230, Percent_Identity=38.695652173913, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1790780, Length=236, Percent_Identity=35.1694915254237, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1789353, Length=228, Percent_Identity=31.5789473684211, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082208, Length=216, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI48994955, Length=210, Percent_Identity=30, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789621, Length=226, Percent_Identity=28.3185840707965, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787821, Length=224, Percent_Identity=27.6785714285714, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994961, Length=213, Percent_Identity=31.924882629108, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26221; Mature: 26221

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRPIRTKKIYEEIVQQIKDLIGEGNLKPGDRLPSERELSERLAVSRASVREALSALAA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGVIVIRPGEGTFVQNIRNGAIVEPLAMALLLDRQAAMELLEARQALEGEAAYLAARRAG
HCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PEDLEKMEELLKEMEHDLQRGILGEEADLRFHLAIAEAARNSVLARLMHTVSDTMRQALK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSRQRLYTTAGNPEKLFAQHNQIYEAIKAHDPRAARKAICQHLRFVEKELQKE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLRPIRTKKIYEEIVQQIKDLIGEGNLKPGDRLPSERELSERLAVSRASVREALSALAA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGVIVIRPGEGTFVQNIRNGAIVEPLAMALLLDRQAAMELLEARQALEGEAAYLAARRAG
HCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PEDLEKMEELLKEMEHDLQRGILGEEADLRFHLAIAEAARNSVLARLMHTVSDTMRQALK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSRQRLYTTAGNPEKLFAQHNQIYEAIKAHDPRAARKAICQHLRFVEKELQKE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]