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The map label for this gene is cwlD [H]

Identifier: 83591117

GI number: 83591117

Start: 2410512

End: 2411261

Strand: Reverse

Name: cwlD [H]

Synonym: Moth_2296

Alternate gene names: 83591117

Gene position: 2411261-2410512 (Counterclockwise)

Preceding gene: 83591120

Following gene: 83591116

Centisome position: 91.73

GC content: 60.8

Gene sequence:

>750_bases
ATGGGGGGAGGCGGGCTCATGGCGCGGGTAGTCTTTGTTCCCCGGCGGCGCGTCCGGGGATTGTTAATAATCGCCCTGGC
GGTGCTGGGTGCCTTCTGGGTCCGGATTTGGTATCAGGGGGTAAAAGAGGAGCAGGCAATTCAGGCCCTTTCCTGGGCGG
TGGCTAACCAGGTGGTGGTGGTCGACCCGGGTCACGGGGGTATCGACGCCGGGGCCATGGGGCCGGGGGGGACGCCGGAA
CACCGGGTTAACTTGGCCATCAGCAAGGACTTGGCCGGCTTTTTAAAAGCCGGTGGGGCGAAGGTTATTCTAACCCGCCA
GGACGATAACGTCCCGCTAGGGGAATCGGGCGACGACCTGGTGGAAAGGGTCCGGCTCGCCGGGAAGGTTGGGGCCGATC
TCTTTATCTCCGTCCACTGCAATGCCTTTGACAGCCGGGAGCGGGGTGCCCAGCTTTTCTATGATCCCAAATCGGCTGAA
GGAAAGAAGCTGGCTGAGTCCATCCAGGCGGAGATTAAGCGCCTGCTGGCCAATACCGACCGGGTACCTTTAAGTATTGA
CGCCTTTGTCCTCCGGACCCAGAAGATCCCTGCGGTTATTGTGGAGGTGGGCTTTATATCCAACCCCGAAGAGGAAAAGC
TGCTGGCTGATCCCCACTACCAGCGCCGCATGGCCTTTGCCATTTACGCCGGCATTGTCAATTACCTGGCGGGGAAGGGA
ACGCCTGCCGGGGGGAAACCGGCAGGTTAA

Upstream 100 bases:

>100_bases
GGCTGAGGAAAGGATCAAGGATTGGCCGCCGCAAATAATTTTTCCTGTCTTGCAGGTAATACTTCCCTGGCGTAGCGAAT
TAATCGGGGGCATATTTATT

Downstream 100 bases:

>100_bases
CAGGTAAAGCTCTGTCACCCCGCGATACCGGGCCAGGAAATTTTGGAAGTTGGGCCGGGTAAAGGTAAAATGGTCCGGGG
TTAGAGCCTGGGCACCGGGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MGGGGLMARVVFVPRRRVRGLLIIALAVLGAFWVRIWYQGVKEEQAIQALSWAVANQVVVVDPGHGGIDAGAMGPGGTPE
HRVNLAISKDLAGFLKAGGAKVILTRQDDNVPLGESGDDLVERVRLAGKVGADLFISVHCNAFDSRERGAQLFYDPKSAE
GKKLAESIQAEIKRLLANTDRVPLSIDAFVLRTQKIPAVIVEVGFISNPEEEKLLADPHYQRRMAFAIYAGIVNYLAGKG
TPAGGKPAG

Sequences:

>Translated_249_residues
MGGGGLMARVVFVPRRRVRGLLIIALAVLGAFWVRIWYQGVKEEQAIQALSWAVANQVVVVDPGHGGIDAGAMGPGGTPE
HRVNLAISKDLAGFLKAGGAKVILTRQDDNVPLGESGDDLVERVRLAGKVGADLFISVHCNAFDSRERGAQLFYDPKSAE
GKKLAESIQAEIKRLLANTDRVPLSIDAFVLRTQKIPAVIVEVGFISNPEEEKLLADPHYQRRMAFAIYAGIVNYLAGKG
TPAGGKPAG
>Mature_248_residues
GGGGLMARVVFVPRRRVRGLLIIALAVLGAFWVRIWYQGVKEEQAIQALSWAVANQVVVVDPGHGGIDAGAMGPGGTPEH
RVNLAISKDLAGFLKAGGAKVILTRQDDNVPLGESGDDLVERVRLAGKVGADLFISVHCNAFDSRERGAQLFYDPKSAEG
KKLAESIQAEIKRLLANTDRVPLSIDAFVLRTQKIPAVIVEVGFISNPEEEKLLADPHYQRRMAFAIYAGIVNYLAGKGT
PAGGKPAG

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=227, Percent_Identity=33.0396475770925, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790611, Length=243, Percent_Identity=29.6296296296296, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082163, Length=99, Percent_Identity=42.4242424242424, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26603; Mature: 26472

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGGGLMARVVFVPRRRVRGLLIIALAVLGAFWVRIWYQGVKEEQAIQALSWAVANQVVV
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEE
VDPGHGGIDAGAMGPGGTPEHRVNLAISKDLAGFLKAGGAKVILTRQDDNVPLGESGDDL
EECCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHH
VERVRLAGKVGADLFISVHCNAFDSRERGAQLFYDPKSAEGKKLAESIQAEIKRLLANTD
HHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
RVPLSIDAFVLRTQKIPAVIVEVGFISNPEEEKLLADPHYQRRMAFAIYAGIVNYLAGKG
CCCEEHHHHEEECCCCCEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TPAGGKPAG
CCCCCCCCC
>Mature Secondary Structure 
GGGGLMARVVFVPRRRVRGLLIIALAVLGAFWVRIWYQGVKEEQAIQALSWAVANQVVV
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEE
VDPGHGGIDAGAMGPGGTPEHRVNLAISKDLAGFLKAGGAKVILTRQDDNVPLGESGDDL
EECCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHH
VERVRLAGKVGADLFISVHCNAFDSRERGAQLFYDPKSAEGKKLAESIQAEIKRLLANTD
HHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
RVPLSIDAFVLRTQKIPAVIVEVGFISNPEEEKLLADPHYQRRMAFAIYAGIVNYLAGKG
CCCEEHHHHEEECCCCCEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TPAGGKPAG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]