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The map label for this gene is ilvB [H]

Identifier: 83591077

GI number: 83591077

Start: 2360836

End: 2362518

Strand: Reverse

Name: ilvB [H]

Synonym: Moth_2256

Alternate gene names: 83591077

Gene position: 2362518-2360836 (Counterclockwise)

Preceding gene: 83591078

Following gene: 83591076

Centisome position: 89.87

GC content: 60.25

Gene sequence:

>1683_bases
ATGGCACAGGAGACTAGCGGCCGCACTGGCGCCCGGGCCGTGGTTGAGGCCCTCCTGGACGAGGGTGTGGAACTGGTCTT
CGGTTATCCCGGCGGGGCGGTATTGCCCCTCTATCATGAGCTGGCCCGGACGCCCATCCGCCATATCCTGGTTCGCCAGG
AGCAGAACGCCGTTCATGCCGCCAGCGGTTACGCCCGCGCCAGCGGCAGAACAGGTGTCTGCTTTGCCACCTCGGGTCCG
GGGGCGACCAACCTGGTCACCGGTATTGCCACCGCTTTTATGGATTCTGTGCCGGTGGTTATCTTTACCGGCCAGGTCCC
GACGCGGATGGTGGGTAGCGATGCCTTCCAGGAAACCGACATTACGGGCATTACCATGCCTATTACCAAGCATAATTACC
TGGTCAAGGATGTAGAGGAATTACCCCGAATCGTCAAGGAGGCCTTCTATATTGCCGGCACCGGTCGCCCGGGACCGGTG
CTGGTAGATATACCCAAGGATGTGGCCCTGGCCCCCTGCCGGGCACCCTTACCGGAAAGGGTGGAGCTGCGGGGTTACAA
ACCCACCTATCATGGCCATCCGGGCCAGCTTCGCTCCCTGGCGCGAATCTTAGGCGAGGCCGAGCGGCCGTTAATCTTCG
CGGGGGGCGGGGTACAGGTTTCCCGAGCCGAGGATTATTTACGCCAACTGGTAGAAAAGCTCCAGATACCGGTAGTAACC
TCCCTCACGGGGCTGGGTTCCTTTCCCGAGGATAATCCTTTATCTTTAGGTATGGTCGGCCTCCATGGCAAGCCCTGCGC
CAACCATGCCCTCATGGAGTGCGACCTCCTGGTGGGCCTGGGGGTACGCTTTGACGACCGGGTAACGGGAGCCCTGGATA
AGTTCGCCCCCCGGGCCAGGATTGCCCACCTGGATATTGACCCGGCGGAAATCGGCAAAAACGTCCGGGTGGATTTACCT
CTGGTAGGCGATATCAGCTGTATCCTGAAGGAACTCCTGCCCCTGGTGGAACCCGCCGGACACGGCCCCTGGCTGCAGCG
CATTAAAGAATTGCGTAATCTCTACCCCCTGACCTATGGCCGCGGCGGCGAGGTGCGGCCCCAGTGGGTAATCGAGCGCC
TGGGGGAGATGACCCGCGGCCAGGCGATTATTACTACCGATGTCGGCCAGCATCAGATGTGGGCAGCCCTCTTTTACGGT
TTTACCGAACCCCGCACCTTCATTTCTTCCTGTGGCCTGGGAACCATGGGTTACGGCCTGCCGGCAGCCGTGGGCGCCGC
CCTGGCCCGGCCCGATAAACAGGTGTGGTTGATAACCGGCGACGGCAGCTTCCAGATGAGCATGGCGGAACTGGGTACAG
CCAGGGAGCAGGGCGTACCTTTAAAGATTTTACTTTTCAATAACCAAAGCCTGGCCATGGTGCGCCAGCTGCAGCACTTT
TACTATGAACGCCAGTATACCGCCATCGAGTTTACCGGCAACCCCGACTTTGTCCGCCTGGCGGAGTGCTACGGGGCCGA
GGGGTTGCGTATAAGTAAGCAGGAAGAAGTGGTGCCAGTCCTGGCTCGGGCTATGGGCAACGACCGCCTGACATTGATTG
AATGCCTGATCAGTCCTGAAGAGATGGTATACCCCATGGTCCCGGAAGGGGCGGCCCTGGACGAGATGATTCTTCCGGAA
TAA

Upstream 100 bases:

>100_bases
GAAAACTCCCTGCGACCCTTCGGCATCCGGGAGGTAGTCCGGACGGGCAAGATCGCCATGCTCCGGGGGGCGAAGACCAG
GGGAATGGAGGCCAGCGGCA

Downstream 100 bases:

>100_bases
TTTTTTTCAGAGGAAGGGCGATGCTTCTTGGCAGATACCGGCAAGCGGATTTATATTTTCGACACGACACTGCGTGACGG
CGAACAGTCGCCGGGGGTAA

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MAQETSGRTGARAVVEALLDEGVELVFGYPGGAVLPLYHELARTPIRHILVRQEQNAVHAASGYARASGRTGVCFATSGP
GATNLVTGIATAFMDSVPVVIFTGQVPTRMVGSDAFQETDITGITMPITKHNYLVKDVEELPRIVKEAFYIAGTGRPGPV
LVDIPKDVALAPCRAPLPERVELRGYKPTYHGHPGQLRSLARILGEAERPLIFAGGGVQVSRAEDYLRQLVEKLQIPVVT
SLTGLGSFPEDNPLSLGMVGLHGKPCANHALMECDLLVGLGVRFDDRVTGALDKFAPRARIAHLDIDPAEIGKNVRVDLP
LVGDISCILKELLPLVEPAGHGPWLQRIKELRNLYPLTYGRGGEVRPQWVIERLGEMTRGQAIITTDVGQHQMWAALFYG
FTEPRTFISSCGLGTMGYGLPAAVGAALARPDKQVWLITGDGSFQMSMAELGTAREQGVPLKILLFNNQSLAMVRQLQHF
YYERQYTAIEFTGNPDFVRLAECYGAEGLRISKQEEVVPVLARAMGNDRLTLIECLISPEEMVYPMVPEGAALDEMILPE

Sequences:

>Translated_560_residues
MAQETSGRTGARAVVEALLDEGVELVFGYPGGAVLPLYHELARTPIRHILVRQEQNAVHAASGYARASGRTGVCFATSGP
GATNLVTGIATAFMDSVPVVIFTGQVPTRMVGSDAFQETDITGITMPITKHNYLVKDVEELPRIVKEAFYIAGTGRPGPV
LVDIPKDVALAPCRAPLPERVELRGYKPTYHGHPGQLRSLARILGEAERPLIFAGGGVQVSRAEDYLRQLVEKLQIPVVT
SLTGLGSFPEDNPLSLGMVGLHGKPCANHALMECDLLVGLGVRFDDRVTGALDKFAPRARIAHLDIDPAEIGKNVRVDLP
LVGDISCILKELLPLVEPAGHGPWLQRIKELRNLYPLTYGRGGEVRPQWVIERLGEMTRGQAIITTDVGQHQMWAALFYG
FTEPRTFISSCGLGTMGYGLPAAVGAALARPDKQVWLITGDGSFQMSMAELGTAREQGVPLKILLFNNQSLAMVRQLQHF
YYERQYTAIEFTGNPDFVRLAECYGAEGLRISKQEEVVPVLARAMGNDRLTLIECLISPEEMVYPMVPEGAALDEMILPE
>Mature_559_residues
AQETSGRTGARAVVEALLDEGVELVFGYPGGAVLPLYHELARTPIRHILVRQEQNAVHAASGYARASGRTGVCFATSGPG
ATNLVTGIATAFMDSVPVVIFTGQVPTRMVGSDAFQETDITGITMPITKHNYLVKDVEELPRIVKEAFYIAGTGRPGPVL
VDIPKDVALAPCRAPLPERVELRGYKPTYHGHPGQLRSLARILGEAERPLIFAGGGVQVSRAEDYLRQLVEKLQIPVVTS
LTGLGSFPEDNPLSLGMVGLHGKPCANHALMECDLLVGLGVRFDDRVTGALDKFAPRARIAHLDIDPAEIGKNVRVDLPL
VGDISCILKELLPLVEPAGHGPWLQRIKELRNLYPLTYGRGGEVRPQWVIERLGEMTRGQAIITTDVGQHQMWAALFYGF
TEPRTFISSCGLGTMGYGLPAAVGAALARPDKQVWLITGDGSFQMSMAELGTAREQGVPLKILLFNNQSLAMVRQLQHFY
YERQYTAIEFTGNPDFVRLAECYGAEGLRISKQEEVVPVLARAMGNDRLTLIECLISPEEMVYPMVPEGAALDEMILPE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.7349823321555, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI21361361, Length=573, Percent_Identity=25.8289703315881, Blast_Score=149, Evalue=6e-36,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=46.113074204947, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=43.9201451905626, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=533, Percent_Identity=33.7711069418386, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=31.021897810219, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=24.0213523131673, Blast_Score=135, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=579, Percent_Identity=24.5250431778929, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=579, Percent_Identity=24.5250431778929, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17542570, Length=528, Percent_Identity=23.4848484848485, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=42.8819444444444, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=485, Percent_Identity=23.5051546391753, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=503, Percent_Identity=23.2604373757455, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922626, Length=558, Percent_Identity=24.1935483870968, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60952; Mature: 60821

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQETSGRTGARAVVEALLDEGVELVFGYPGGAVLPLYHELARTPIRHILVRQEQNAVHA
CCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHCCCCCEEH
ASGYARASGRTGVCFATSGPGATNLVTGIATAFMDSVPVVIFTGQVPTRMVGSDAFQETD
HCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCHHCCCC
ITGITMPITKHNYLVKDVEELPRIVKEAFYIAGTGRPGPVLVDIPKDVALAPCRAPLPER
CCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCEECCCCCCCCCC
VELRGYKPTYHGHPGQLRSLARILGEAERPLIFAGGGVQVSRAEDYLRQLVEKLQIPVVT
EEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCEEEHHHHHHHHHHHHHHCCCCEE
SLTGLGSFPEDNPLSLGMVGLHGKPCANHALMECDLLVGLGVRFDDRVTGALDKFAPRAR
HHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHEEHHHEECCCCCCCHHHHHHHHCCCCCE
IAHLDIDPAEIGKNVRVDLPLVGDISCILKELLPLVEPAGHGPWLQRIKELRNLYPLTYG
EEEECCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
RGGEVRPQWVIERLGEMTRGQAIITTDVGQHQMWAALFYGFTEPRTFISSCGLGTMGYGL
CCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCH
PAAVGAALARPDKQVWLITGDGSFQMSMAELGTAREQGVPLKILLFNNQSLAMVRQLQHF
HHHHHHHHCCCCCEEEEEECCCCEEEEHHHHCCHHHCCCCEEEEEECCCHHHHHHHHHHH
YYERQYTAIEFTGNPDFVRLAECYGAEGLRISKQEEVVPVLARAMGNDRLTLIECLISPE
HHHCEEEEEEECCCCCEEEHHHHCCCCCCEECCHHHHHHHHHHHHCCCCEEEHHHHCCHH
EMVYPMVPEGAALDEMILPE
HHCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
AQETSGRTGARAVVEALLDEGVELVFGYPGGAVLPLYHELARTPIRHILVRQEQNAVHA
CCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHCCCCCEEH
ASGYARASGRTGVCFATSGPGATNLVTGIATAFMDSVPVVIFTGQVPTRMVGSDAFQETD
HCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCHHCCCC
ITGITMPITKHNYLVKDVEELPRIVKEAFYIAGTGRPGPVLVDIPKDVALAPCRAPLPER
CCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCEECCCCCCCCCC
VELRGYKPTYHGHPGQLRSLARILGEAERPLIFAGGGVQVSRAEDYLRQLVEKLQIPVVT
EEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCEEEHHHHHHHHHHHHHHCCCCEE
SLTGLGSFPEDNPLSLGMVGLHGKPCANHALMECDLLVGLGVRFDDRVTGALDKFAPRAR
HHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHEEHHHEECCCCCCCHHHHHHHHCCCCCE
IAHLDIDPAEIGKNVRVDLPLVGDISCILKELLPLVEPAGHGPWLQRIKELRNLYPLTYG
EEEECCCHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
RGGEVRPQWVIERLGEMTRGQAIITTDVGQHQMWAALFYGFTEPRTFISSCGLGTMGYGL
CCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCH
PAAVGAALARPDKQVWLITGDGSFQMSMAELGTAREQGVPLKILLFNNQSLAMVRQLQHF
HHHHHHHHCCCCCEEEEEECCCCEEEEHHHHCCHHHCCCCEEEEEECCCHHHHHHHHHHH
YYERQYTAIEFTGNPDFVRLAECYGAEGLRISKQEEVVPVLARAMGNDRLTLIECLISPE
HHHCEEEEEEECCCCCEEEHHHHCCCCCCEECCHHHHHHHHHHHHCCCCEEEHHHHCCHH
EMVYPMVPEGAALDEMILPE
HHCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]