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The map label for this gene is leuA [H]

Identifier: 83591076

GI number: 83591076

Start: 2359255

End: 2360808

Strand: Reverse

Name: leuA [H]

Synonym: Moth_2255

Alternate gene names: 83591076

Gene position: 2360808-2359255 (Counterclockwise)

Preceding gene: 83591077

Following gene: 83591075

Centisome position: 89.81

GC content: 59.52

Gene sequence:

>1554_bases
TTGGCAGATACCGGCAAGCGGATTTATATTTTCGACACGACACTGCGTGACGGCGAACAGTCGCCGGGGGTAAGCCTCAA
TATCCAGGAAAAGCTGGAGATTGCCCGCCAGCTGGCCCGCCTGGGGGTCGATGTTATTGAAGCCGGCTTTCCCATCGCCT
CCCCCGGGGACTTTGAGGCCGTCCGGGCCGTGGCTCGAGAGGTGGAGGGACCGGTCATCGCCGGCCTGGCCCGGATTAAC
GAACGGGATATCGACCGCGCCTGGGAGGCCCTCCAGGAGGCCCAAAAGCCGCGGATCCACGTCTTTATTGCCACCTCCGA
TATCCACCTGAAATACAAACTGCGGATGAGCCGGCAGGAGGTCCTGGAGGCTGTCCGGCGGGGAGTGGCCCGGGCCAAGG
GTTACTGCCAGGATATTGAGTTTTCTCCGGAGGATGCTGTCCGGAGCGACCTGGACTTCCTCTGCCAGGTCCTGGCCACG
GCCATTGAGGCCGGGGCTACCGTTCTTAATATCCCGGATACCGTGGGCTACGCTACCCCGGAGGAGTTCGGCCGTCTGAT
CAGCCAGATCCGGCAGCGGGTGCCGGGTATTGATAAAGTCCGGATCAGCGTTCACTGCCATAACGATCTGGGCCTGGCCG
TGGCCAACTCCCTGGCGGCCATTGAGAACGGCGCCCTGCAGGTCGAGGGGGCCATCAATGGCATTGGCGAAAGGGCTGGA
AATGCCGCCCTGGAGGAAGTGATTATGGCCCTTTATACCCGGCGGGATTACTACGGCTGCCGGACGGGCATCGTCACCGA
GGAGATCTACCGCACCAGCAAACTGGTCAGTAGCTTGACGGGTATGCCCGTCCAGTACAATAAAGCCATTGTCGGCAAGA
ACGCCTTCACCCATGAGGCCGGCATTCACCAGGACGGGGTCTTAAAAGAGCGGACTACCTACGAAATCATGAATCCGGCC
ATGATCGGCCTGGTCCAGAATAATATTTTCCTGGGCAAGCACTCCGGCCGCCACGCCCTGCGTAGCCGCCTGGAGGAACT
GGGCTTTAAGCTCACGGAGGCCGAGCTGGATAAGGCCTTCGCCCGCTTCAAAGAACTGGCCGACCGCAAGAAAGAAATCA
GCGACCGCGACCTGGAGGCCATTGTCGAGCACGAAGTCAAGCGCATCCCGGAGAAGTTTGTTCTAGAGCATATTCATATC
TCCACCGGCAACCGGGTTGTACCCACAGCCACCATCGGTATCCGGGTGGGTGAAGAATTAAAGGAAGAGGCCGCCTGCGG
TGAAGGCCCGGTAGACGCCGCCTTCAAGGCCATAGATAAGCTGACCCGCATCCCGGTTTGCCTCAAATCTTACAATCTCA
ATGCCGTCACCGGCGGCAAGGACGCCGTCGGTGAGGTAACGGTAAAGATTGAGTACGATGGGCGGGTCTTCATAGGTCGC
GGTATCAGCACCGATGTCCTGGAAGCCAGTGCCCGCGCTTACTTGAACGCCATCAATAAGGTGGTTTACGAGGTAGGGGA
AGAAAACCTGCAGCAGGCCAGTACGGCCCAGTAG

Upstream 100 bases:

>100_bases
CAGTCCTGAAGAGATGGTATACCCCATGGTCCCGGAAGGGGCGGCCCTGGACGAGATGATTCTTCCGGAATAATTTTTTT
CAGAGGAAGGGCGATGCTTC

Downstream 100 bases:

>100_bases
AACCGAACTGGATAGAGTGCCATCAAAGGACTATGTCCTGCAGCCGGTAGGCGATAAATATTTGGCAAGACAGGTTACCG
ATAACTTCAGGCAAGATTAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MADTGKRIYIFDTTLRDGEQSPGVSLNIQEKLEIARQLARLGVDVIEAGFPIASPGDFEAVRAVAREVEGPVIAGLARIN
ERDIDRAWEALQEAQKPRIHVFIATSDIHLKYKLRMSRQEVLEAVRRGVARAKGYCQDIEFSPEDAVRSDLDFLCQVLAT
AIEAGATVLNIPDTVGYATPEEFGRLISQIRQRVPGIDKVRISVHCHNDLGLAVANSLAAIENGALQVEGAINGIGERAG
NAALEEVIMALYTRRDYYGCRTGIVTEEIYRTSKLVSSLTGMPVQYNKAIVGKNAFTHEAGIHQDGVLKERTTYEIMNPA
MIGLVQNNIFLGKHSGRHALRSRLEELGFKLTEAELDKAFARFKELADRKKEISDRDLEAIVEHEVKRIPEKFVLEHIHI
STGNRVVPTATIGIRVGEELKEEAACGEGPVDAAFKAIDKLTRIPVCLKSYNLNAVTGGKDAVGEVTVKIEYDGRVFIGR
GISTDVLEASARAYLNAINKVVYEVGEENLQQASTAQ

Sequences:

>Translated_517_residues
MADTGKRIYIFDTTLRDGEQSPGVSLNIQEKLEIARQLARLGVDVIEAGFPIASPGDFEAVRAVAREVEGPVIAGLARIN
ERDIDRAWEALQEAQKPRIHVFIATSDIHLKYKLRMSRQEVLEAVRRGVARAKGYCQDIEFSPEDAVRSDLDFLCQVLAT
AIEAGATVLNIPDTVGYATPEEFGRLISQIRQRVPGIDKVRISVHCHNDLGLAVANSLAAIENGALQVEGAINGIGERAG
NAALEEVIMALYTRRDYYGCRTGIVTEEIYRTSKLVSSLTGMPVQYNKAIVGKNAFTHEAGIHQDGVLKERTTYEIMNPA
MIGLVQNNIFLGKHSGRHALRSRLEELGFKLTEAELDKAFARFKELADRKKEISDRDLEAIVEHEVKRIPEKFVLEHIHI
STGNRVVPTATIGIRVGEELKEEAACGEGPVDAAFKAIDKLTRIPVCLKSYNLNAVTGGKDAVGEVTVKIEYDGRVFIGR
GISTDVLEASARAYLNAINKVVYEVGEENLQQASTAQ
>Mature_516_residues
ADTGKRIYIFDTTLRDGEQSPGVSLNIQEKLEIARQLARLGVDVIEAGFPIASPGDFEAVRAVAREVEGPVIAGLARINE
RDIDRAWEALQEAQKPRIHVFIATSDIHLKYKLRMSRQEVLEAVRRGVARAKGYCQDIEFSPEDAVRSDLDFLCQVLATA
IEAGATVLNIPDTVGYATPEEFGRLISQIRQRVPGIDKVRISVHCHNDLGLAVANSLAAIENGALQVEGAINGIGERAGN
AALEEVIMALYTRRDYYGCRTGIVTEEIYRTSKLVSSLTGMPVQYNKAIVGKNAFTHEAGIHQDGVLKERTTYEIMNPAM
IGLVQNNIFLGKHSGRHALRSRLEELGFKLTEAELDKAFARFKELADRKKEISDRDLEAIVEHEVKRIPEKFVLEHIHIS
TGNRVVPTATIGIRVGEELKEEAACGEGPVDAAFKAIDKLTRIPVCLKSYNLNAVTGGKDAVGEVTVKIEYDGRVFIGRG
ISTDVLEASARAYLNAINKVVYEVGEENLQQASTAQ

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=497, Percent_Identity=50.9054325955734, Blast_Score=496, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320071, Length=380, Percent_Identity=31.8421052631579, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320019, Length=376, Percent_Identity=31.3829787234043, Blast_Score=156, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6324225, Length=556, Percent_Identity=25.3597122302158, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324682, Length=545, Percent_Identity=25.1376146788991, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24582381, Length=297, Percent_Identity=25.9259259259259, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56829; Mature: 56697

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTGKRIYIFDTTLRDGEQSPGVSLNIQEKLEIARQLARLGVDVIEAGFPIASPGDFEA
CCCCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VRAVAREVEGPVIAGLARINERDIDRAWEALQEAQKPRIHVFIATSDIHLKYKLRMSRQE
HHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEEECCCHHH
VLEAVRRGVARAKGYCQDIEFSPEDAVRSDLDFLCQVLATAIEAGATVLNIPDTVGYATP
HHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCH
EEFGRLISQIRQRVPGIDKVRISVHCHNDLGLAVANSLAAIENGALQVEGAINGIGERAG
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCC
NAALEEVIMALYTRRDYYGCRTGIVTEEIYRTSKLVSSLTGMPVQYNKAIVGKNAFTHEA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCCC
GIHQDGVLKERTTYEIMNPAMIGLVQNNIFLGKHSGRHALRSRLEELGFKLTEAELDKAF
CCCCCCCCCCCCHHHHCCCHHEEEEECCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
ARFKELADRKKEISDRDLEAIVEHEVKRIPEKFVLEHIHISTGNRVVPTATIGIRVGEEL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHEEECCCCEEEEEEEEEEECCHHH
KEEAACGEGPVDAAFKAIDKLTRIPVCLKSYNLNAVTGGKDAVGEVTVKIEYDGRVFIGR
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCEECCCCCCCCEEEEEEEECCEEEEEC
GISTDVLEASARAYLNAINKVVYEVGEENLQQASTAQ
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure 
ADTGKRIYIFDTTLRDGEQSPGVSLNIQEKLEIARQLARLGVDVIEAGFPIASPGDFEA
CCCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHH
VRAVAREVEGPVIAGLARINERDIDRAWEALQEAQKPRIHVFIATSDIHLKYKLRMSRQE
HHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEEECCCHHH
VLEAVRRGVARAKGYCQDIEFSPEDAVRSDLDFLCQVLATAIEAGATVLNIPDTVGYATP
HHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCH
EEFGRLISQIRQRVPGIDKVRISVHCHNDLGLAVANSLAAIENGALQVEGAINGIGERAG
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCC
NAALEEVIMALYTRRDYYGCRTGIVTEEIYRTSKLVSSLTGMPVQYNKAIVGKNAFTHEA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCEEECCCCCCCCC
GIHQDGVLKERTTYEIMNPAMIGLVQNNIFLGKHSGRHALRSRLEELGFKLTEAELDKAF
CCCCCCCCCCCCHHHHCCCHHEEEEECCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
ARFKELADRKKEISDRDLEAIVEHEVKRIPEKFVLEHIHISTGNRVVPTATIGIRVGEEL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHEEECCCCEEEEEEEEEEECCHHH
KEEAACGEGPVDAAFKAIDKLTRIPVCLKSYNLNAVTGGKDAVGEVTVKIEYDGRVFIGR
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCEECCCCCCCCEEEEEEEECCEEEEEC
GISTDVLEASARAYLNAINKVVYEVGEENLQQASTAQ
CCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA