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The map label for this gene is leuB

Identifier: 83591073

GI number: 83591073

Start: 2356159

End: 2357232

Strand: Reverse

Name: leuB

Synonym: Moth_2252

Alternate gene names: 83591073

Gene position: 2357232-2356159 (Counterclockwise)

Preceding gene: 83591074

Following gene: 83591072

Centisome position: 89.67

GC content: 62.66

Gene sequence:

>1074_bases
GTGTATAAAATCGCCGTTCTGCCCGGGGATGGTATCGGCCCGGAGATCGTCCCCGAGGCCGTCAAGGTCCTGGAGGCAGT
CAGCCGCCGGGTGGGTATTGAATTCCAGTTTACCGAAGCGCTGGTAGGCGGCGCTGCCATCGATGCCCGGGGGATAGCCC
TGCCGCCGGAGACCCTGGAGCTCTGCCGCCAGAGCGATGCCGTGCTCCTGGGGGCGGTAGGCGGTCCGAAATGGGATACC
CTGCCCCCGGCAGAGCGCCCGGAGACGGCAGCCCTGCTGCCCCTGCGTAAAGAACTGGGCCTCTATGCCAACCTGCGCCC
GGCTTTTCTCTATGATTCCCTGGTGGAGGCCTCGCCCCTGAAGAAGGAAATAGTCACCGGCACCGACCTGATTATCGTCC
GGGAACTGACGGGGGGGCTTTACTTCGGCGCCAAGAAACGAGAGCAGACCGCCGAGGGGGAAATGGCCTATGATACCATG
TATTACACAAGGGCGGAGATTGAACGCATCGTCCGGCTGGCCTTTACCATAGCCCGCCAGCGGCGCTGCCACCTGACCAG
CGTCGACAAGGCCAACGTCCTCACCACCTCGCGCCTGTGGCGGGATACGGTGGAGGATATCAAAGGCGAGTTCCCCGAGG
TAACGGTGGAGCACATGTATGTCGACAACTGCGCCATGCAGCTGGTGCGCCGCCCGGCCCAGTTTGACGTAATTGTGACC
GAGAATACCTTTGGCGATATCCTGAGTGATCAGGCTTCCGTCCTGACGGGTTCCATTGGCATGTTGCCCTCGGCCAGCAT
CGGCGGTGCCGTGGCCCTCTATGAACCCTGCCACGGCTCGGCACCGGACATTGCCGGCCAGCAGAAAGCCAATCCCCTGG
CGACCATCCTTTCGGCAGCCATGATGCTGAAGTACTCCTTTAAAATGGACCAGGCGGCGGCAGCCATTGAGGCGGCAGTA
GGCAGGGTCCTGGCCAAGGGCTACCGGACCCCGGACCTCTATGTCCCGGGGACCCAACTGGTTGGTACCGCCGAAATGGG
GCAACTCGTCCGGCGGGAGCTGGAAGAGGGATAA

Upstream 100 bases:

>100_bases
AGGGGCGAGACCTACCCGGTGGCCCCCTTCCCACCCTTTATGCAGGAACTCATTGCCGCCGGGGGATTGATGCCCTACGT
GGCCAGGAAGGTGAAGACCA

Downstream 100 bases:

>100_bases
GAGAATGGCCGAGAAGATCCTTATCTACGATACCACCCTCCGGGACGGCAGCCAGGGCGAGGGTATCAGCCTGTCGGTAG
AGGATAAACTAAAGATTGCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MYKIAVLPGDGIGPEIVPEAVKVLEAVSRRVGIEFQFTEALVGGAAIDARGIALPPETLELCRQSDAVLLGAVGGPKWDT
LPPAERPETAALLPLRKELGLYANLRPAFLYDSLVEASPLKKEIVTGTDLIIVRELTGGLYFGAKKREQTAEGEMAYDTM
YYTRAEIERIVRLAFTIARQRRCHLTSVDKANVLTTSRLWRDTVEDIKGEFPEVTVEHMYVDNCAMQLVRRPAQFDVIVT
ENTFGDILSDQASVLTGSIGMLPSASIGGAVALYEPCHGSAPDIAGQQKANPLATILSAAMMLKYSFKMDQAAAAIEAAV
GRVLAKGYRTPDLYVPGTQLVGTAEMGQLVRRELEEG

Sequences:

>Translated_357_residues
MYKIAVLPGDGIGPEIVPEAVKVLEAVSRRVGIEFQFTEALVGGAAIDARGIALPPETLELCRQSDAVLLGAVGGPKWDT
LPPAERPETAALLPLRKELGLYANLRPAFLYDSLVEASPLKKEIVTGTDLIIVRELTGGLYFGAKKREQTAEGEMAYDTM
YYTRAEIERIVRLAFTIARQRRCHLTSVDKANVLTTSRLWRDTVEDIKGEFPEVTVEHMYVDNCAMQLVRRPAQFDVIVT
ENTFGDILSDQASVLTGSIGMLPSASIGGAVALYEPCHGSAPDIAGQQKANPLATILSAAMMLKYSFKMDQAAAAIEAAV
GRVLAKGYRTPDLYVPGTQLVGTAEMGQLVRRELEEG
>Mature_357_residues
MYKIAVLPGDGIGPEIVPEAVKVLEAVSRRVGIEFQFTEALVGGAAIDARGIALPPETLELCRQSDAVLLGAVGGPKWDT
LPPAERPETAALLPLRKELGLYANLRPAFLYDSLVEASPLKKEIVTGTDLIIVRELTGGLYFGAKKREQTAEGEMAYDTM
YYTRAEIERIVRLAFTIARQRRCHLTSVDKANVLTTSRLWRDTVEDIKGEFPEVTVEHMYVDNCAMQLVRRPAQFDVIVT
ENTFGDILSDQASVLTGSIGMLPSASIGGAVALYEPCHGSAPDIAGQQKANPLATILSAAMMLKYSFKMDQAAAAIEAAV
GRVLAKGYRTPDLYVPGTQLVGTAEMGQLVRRELEEG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=334, Percent_Identity=33.2335329341317, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI4758582, Length=357, Percent_Identity=30.812324929972, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI28178838, Length=322, Percent_Identity=31.055900621118, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI28178821, Length=310, Percent_Identity=30.3225806451613, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178816, Length=310, Percent_Identity=30.3225806451613, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI28178819, Length=148, Percent_Identity=37.1621621621622, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=359, Percent_Identity=54.874651810585, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1788101, Length=343, Percent_Identity=37.9008746355685, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=29, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71986051, Length=352, Percent_Identity=32.6704545454545, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI25144293, Length=359, Percent_Identity=30.6406685236769, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17550882, Length=339, Percent_Identity=29.7935103244838, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=315, Percent_Identity=29.8412698412698, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=52.1857923497268, Blast_Score=336, Evalue=3e-93,
Organism=Saccharomyces cerevisiae, GI6322097, Length=344, Percent_Identity=36.046511627907, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6324709, Length=339, Percent_Identity=34.2182890855457, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=334, Percent_Identity=31.7365269461078, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24643268, Length=337, Percent_Identity=33.5311572700297, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24643270, Length=337, Percent_Identity=33.5311572700297, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24661184, Length=340, Percent_Identity=29.1176470588235, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161078635, Length=344, Percent_Identity=27.0348837209302, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI161078637, Length=344, Percent_Identity=27.0348837209302, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078633, Length=344, Percent_Identity=27.0348837209302, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24650122, Length=344, Percent_Identity=27.0348837209302, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078639, Length=340, Percent_Identity=27.3529411764706, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281362242, Length=332, Percent_Identity=28.0120481927711, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648872, Length=332, Percent_Identity=28.0120481927711, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130355, Length=356, Percent_Identity=23.876404494382, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_MOOTA (Q2RGA0)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431082.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q2RGA0
- SMR:   Q2RGA0
- STRING:   Q2RGA0
- GeneID:   3830747
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2252
- NMPDR:   fig|264732.9.peg.2283
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- PhylomeDB:   Q2RGA0
- ProtClustDB:   PRK00772
- BioCyc:   MTHE264732:MOTH_2252-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38698; Mature: 38698

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 134-134 BINDING 222-222

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKIAVLPGDGIGPEIVPEAVKVLEAVSRRVGIEFQFTEALVGGAAIDARGIALPPETLE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHCCCCCCCCCCCCCHHHHH
LCRQSDAVLLGAVGGPKWDTLPPAERPETAALLPLRKELGLYANLRPAFLYDSLVEASPL
HHCCCCCEEEECCCCCCCCCCCCCCCCCCCEECHHHHHCCCCCCCCHHHHHHHHHHCCCH
KKEIVTGTDLIIVRELTGGLYFGAKKREQTAEGEMAYDTMYYTRAEIERIVRLAFTIARQ
HHHHCCCCCEEEEEECCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRCHLTSVDKANVLTTSRLWRDTVEDIKGEFPEVTVEHMYVDNCAMQLVRRPAQFDVIVT
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEE
ENTFGDILSDQASVLTGSIGMLPSASIGGAVALYEPCHGSAPDIAGQQKANPLATILSAA
CCCHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
MMLKYSFKMDQAAAAIEAAVGRVLAKGYRTPDLYVPGTQLVGTAEMGQLVRRELEEG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEECHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYKIAVLPGDGIGPEIVPEAVKVLEAVSRRVGIEFQFTEALVGGAAIDARGIALPPETLE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHCCCCCCCCCCCCCHHHHH
LCRQSDAVLLGAVGGPKWDTLPPAERPETAALLPLRKELGLYANLRPAFLYDSLVEASPL
HHCCCCCEEEECCCCCCCCCCCCCCCCCCCEECHHHHHCCCCCCCCHHHHHHHHHHCCCH
KKEIVTGTDLIIVRELTGGLYFGAKKREQTAEGEMAYDTMYYTRAEIERIVRLAFTIARQ
HHHHCCCCCEEEEEECCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRCHLTSVDKANVLTTSRLWRDTVEDIKGEFPEVTVEHMYVDNCAMQLVRRPAQFDVIVT
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEE
ENTFGDILSDQASVLTGSIGMLPSASIGGAVALYEPCHGSAPDIAGQQKANPLATILSAA
CCCHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
MMLKYSFKMDQAAAAIEAAVGRVLAKGYRTPDLYVPGTQLVGTAEMGQLVRRELEEG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA