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The map label for this gene is leuD

Identifier: 83591074

GI number: 83591074

Start: 2357225

End: 2357725

Strand: Reverse

Name: leuD

Synonym: Moth_2253

Alternate gene names: 83591074

Gene position: 2357725-2357225 (Counterclockwise)

Preceding gene: 83591075

Following gene: 83591073

Centisome position: 89.69

GC content: 62.28

Gene sequence:

>501_bases
ATGATCATTCAGGGCAAGTGCCACACCTTCGGCAACGACATCGACACCGACGCCATCATCCCGGCGCGGTATTTAAATAC
CACCGACCCCGGGGAACTGGCCCGGCACTGTATGGAAGACGCCGACCCCACCTTTGCCGGCCGGGTGCAAAAAGGGGAGA
TAATCGTGGCCGGGAAGAACTTCGGCTGCGGCAGTTCCCGGGAACACGCGCCGGTGGCCATCAAGGCCGCCGGGGTGGCG
GCGGTCGTCGCCGCCTCCTTCGCCCGCATCTTCTATCGCAACGCCATCAACATCGGCCTGCCCATCTTCGAGTCCCCGGA
GGCAGCGGCGGGGATCAAGGCCGGTGACGAGGTAAAGATTGATGCCGGAAAGGGAGAAATAGTCAACCTCACCAGGGGCG
AGACCTACCCGGTGGCCCCCTTCCCACCCTTTATGCAGGAACTCATTGCCGCCGGGGGATTGATGCCCTACGTGGCCAGG
AAGGTGAAGACCAGTGTATAA

Upstream 100 bases:

>100_bases
GCATGGGCCATCCCGAGAGCGAAGTCTATCTCGCCGGCCCGGCGGTAGCCGCCGCCAGCGCCGTTAAAGGGCGCATCGCC
GCCCCAGAGGAGGTAGTAAA

Downstream 100 bases:

>100_bases
AATCGCCGTTCTGCCCGGGGATGGTATCGGCCCGGAGATCGTCCCCGAGGCCGTCAAGGTCCTGGAGGCAGTCAGCCGCC
GGGTGGGTATTGAATTCCAG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MIIQGKCHTFGNDIDTDAIIPARYLNTTDPGELARHCMEDADPTFAGRVQKGEIIVAGKNFGCGSSREHAPVAIKAAGVA
AVVAASFARIFYRNAINIGLPIFESPEAAAGIKAGDEVKIDAGKGEIVNLTRGETYPVAPFPPFMQELIAAGGLMPYVAR
KVKTSV

Sequences:

>Translated_166_residues
MIIQGKCHTFGNDIDTDAIIPARYLNTTDPGELARHCMEDADPTFAGRVQKGEIIVAGKNFGCGSSREHAPVAIKAAGVA
AVVAASFARIFYRNAINIGLPIFESPEAAAGIKAGDEVKIDAGKGEIVNLTRGETYPVAPFPPFMQELIAAGGLMPYVAR
KVKTSV
>Mature_166_residues
MIIQGKCHTFGNDIDTDAIIPARYLNTTDPGELARHCMEDADPTFAGRVQKGEIIVAGKNFGCGSSREHAPVAIKAAGVA
AVVAASFARIFYRNAINIGLPIFESPEAAAGIKAGDEVKIDAGKGEIVNLTRGETYPVAPFPPFMQELIAAGGLMPYVAR
KVKTSV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=127, Percent_Identity=36.2204724409449, Blast_Score=72, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=98, Percent_Identity=45.9183673469388, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=36.8932038834951, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_MOOTA (Q2RG99)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431083.1
- ProteinModelPortal:   Q2RG99
- SMR:   Q2RG99
- STRING:   Q2RG99
- GeneID:   3830748
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2253
- NMPDR:   fig|264732.9.peg.2284
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   PFPEFMQ
- ProtClustDB:   PRK00439
- BioCyc:   MTHE264732:MOTH_2253-MONOMER
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17516; Mature: 17516

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIQGKCHTFGNDIDTDAIIPARYLNTTDPGELARHCMEDADPTFAGRVQKGEIIVAGKN
CEECCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCEECCCCEEEEECC
FGCGSSREHAPVAIKAAGVAAVVAASFARIFYRNAINIGLPIFESPEAAAGIKAGDEVKI
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
DAGKGEIVNLTRGETYPVAPFPPFMQELIAAGGLMPYVARKVKTSV
ECCCCEEEEECCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIIQGKCHTFGNDIDTDAIIPARYLNTTDPGELARHCMEDADPTFAGRVQKGEIIVAGKN
CEECCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCEECCCCEEEEECC
FGCGSSREHAPVAIKAAGVAAVVAASFARIFYRNAINIGLPIFESPEAAAGIKAGDEVKI
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
DAGKGEIVNLTRGETYPVAPFPPFMQELIAAGGLMPYVARKVKTSV
ECCCCEEEEECCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA