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The map label for this gene is hycE [H]

Identifier: 83591009

GI number: 83591009

Start: 2283537

End: 2285261

Strand: Reverse

Name: hycE [H]

Synonym: Moth_2186

Alternate gene names: 83591009

Gene position: 2285261-2283537 (Counterclockwise)

Preceding gene: 83591010

Following gene: 83591008

Centisome position: 86.93

GC content: 54.96

Gene sequence:

>1725_bases
ATGGGACAGGGTTCTGAGTCCAAGCGGGCACAAAAATATATAAAAGATTTGCGGTCGAAATTCCCGGGGGCGATCCTGGA
GGAAAGCTCCCAGGCTCCAGACCAGATTACTGTGACAGTGAAACTTAACGACCTTCCCGGCATCGTGGAAGAGCTATATT
ATCGTCATAATGGCTGGCTATCCACCATGCTCGGTAACGACGAGCGCGAGCTTAACGGTTGTTTCGCCCTCTATTATGTC
TTGTCCATGGAAGGGAACAGCAACAAAAGGGAGAACACCTGGATAACCGTCAAAGCCCTGGTACCCGAGAAAGAGCCGCA
ATTTCCATCGGTAACTCCCCGGGTGCCGGCGGCGGTGTGGTATGAGCGGGAAGTGCGGGACATGTTCGGCCTGGAACCGG
TGGGAATCCCAGATGCGCGGAGGCTGGTCTTGCCTGATGATTGGCCGGACAACTTGCATCCGTTGCGAAAAGACGCCATG
GATTACCGGTACCGCCCGGAACCTGTTGAGGAGAACTACAATTTTATCGAAGTCGAAGGGGAGGGAATTGTGGAAGTTCC
ACTGGGACCCCTCCACATCACCTCCGATGAACCCGGACATTTCCGCCTCTTTGTTGACGGGGAGTACATCGTGGATGCTG
ACTACCGGCTCTTTTATGTACACCGGGGTATGGAGAAACTGGCCGAAAACCGTATGGATTACGACCAAATTACCTTCCTG
GCCGAGCGGATTTGCGGCATCTGCGGCTACGCCCACAGTGTGGCGTATGCTGCGGCGGTGGAAACGGCCAACGGAATTGA
GGTGCCGCCGCGGGCTCAGTATATCCGCACCATTTTGCTAGAAGTGGAGAGGTTGCACAGCCACCTCTTGAATCTGGGGC
TGGCGGCCCATCTGGTGGGTTTCGATTCGGGTTTCATGCATTTCTTCCGGGTACGGGAGAAGGCCATGCAGATGGCCGAG
ATTCTTACCGGGGGCCGGAAAACCTATGGAATAAACCTGATTGGCGGCGTTCGCCGGGATATTTTCAAAGAAGAGCGGGA
CCAGGTCTTACGGCTGATTGCGGAGATCCGGACCGAACTGGATGAACTACTTGATATCCTGATAAACACCCCCAACTTCA
TCTCGCGGACCCAGGGGGTAGGCCGCCTGGAACGCCAGGTAGCCCGTGACTTCAGCCCGGTTGGTCCTAATATGCGGGGT
TCCGGGTACGCCCGTGATACCAGAGCGGACCACCCCTACGGCGCTTACGACCGGGTATCCTGGGAGGTCATTTCCAAGGA
CGGTTGCGACGTACTTTCCAGGGAACTGGTCCGGGCAGCAGAGCTGTATGAGTCCTTCAATATAATCGAGAGGTGCTTGA
CCGAAATGCCCCCCGGCCCGGTTCTCACGGAAGGGTTTGCGTATAAACCGCATACCTTCGCGTTGGGATATACTGAAGCC
CCGAGGGGAGAAAATGTCCACTGGCTGATGACGGGCAACAACCAGAAACTTTATCGCTGGCGGGTACGGGCTGCCACCTA
CAATAACTGGCCGGCGTTACGGTATATGTTTAGAGGCAACACCGTGTCTGATGCCCCCCTGATTGTGGCCAGCATCGATC
CCTGCTATTCCTGCACCGAAAGGGTCACTATGGTCGATGTGCGTAAAAAGAAGGCGAGAACGATTGAATACAAAGAACTG
GAGCGTTACTGCCGGGAAAGAAAATACTCGCCGCTTAAATTTTAG

Upstream 100 bases:

>100_bases
TGGGATAGGGGAACATTTATCATGACCAAAACGGCTGAGATGCCCTTGAAGATTGATTTGGCAGTTTTTTGGGGGAAATT
ATATCGGGAGGTAGATGAAG

Downstream 100 bases:

>100_bases
CCTCGTTGATATACTGGCGGAAGAAGGGGGATATATGAATGCTAAAACTGCTTAAAAAAGCACTGCAGGTCGGGGAAGCC
ACGGTTGAGTATCCCTTTAA

Product: NADH dehydrogenase (ubiquinone), 30 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MGQGSESKRAQKYIKDLRSKFPGAILEESSQAPDQITVTVKLNDLPGIVEELYYRHNGWLSTMLGNDERELNGCFALYYV
LSMEGNSNKRENTWITVKALVPEKEPQFPSVTPRVPAAVWYEREVRDMFGLEPVGIPDARRLVLPDDWPDNLHPLRKDAM
DYRYRPEPVEENYNFIEVEGEGIVEVPLGPLHITSDEPGHFRLFVDGEYIVDADYRLFYVHRGMEKLAENRMDYDQITFL
AERICGICGYAHSVAYAAAVETANGIEVPPRAQYIRTILLEVERLHSHLLNLGLAAHLVGFDSGFMHFFRVREKAMQMAE
ILTGGRKTYGINLIGGVRRDIFKEERDQVLRLIAEIRTELDELLDILINTPNFISRTQGVGRLERQVARDFSPVGPNMRG
SGYARDTRADHPYGAYDRVSWEVISKDGCDVLSRELVRAAELYESFNIIERCLTEMPPGPVLTEGFAYKPHTFALGYTEA
PRGENVHWLMTGNNQKLYRWRVRAATYNNWPALRYMFRGNTVSDAPLIVASIDPCYSCTERVTMVDVRKKKARTIEYKEL
ERYCRERKYSPLKF

Sequences:

>Translated_574_residues
MGQGSESKRAQKYIKDLRSKFPGAILEESSQAPDQITVTVKLNDLPGIVEELYYRHNGWLSTMLGNDERELNGCFALYYV
LSMEGNSNKRENTWITVKALVPEKEPQFPSVTPRVPAAVWYEREVRDMFGLEPVGIPDARRLVLPDDWPDNLHPLRKDAM
DYRYRPEPVEENYNFIEVEGEGIVEVPLGPLHITSDEPGHFRLFVDGEYIVDADYRLFYVHRGMEKLAENRMDYDQITFL
AERICGICGYAHSVAYAAAVETANGIEVPPRAQYIRTILLEVERLHSHLLNLGLAAHLVGFDSGFMHFFRVREKAMQMAE
ILTGGRKTYGINLIGGVRRDIFKEERDQVLRLIAEIRTELDELLDILINTPNFISRTQGVGRLERQVARDFSPVGPNMRG
SGYARDTRADHPYGAYDRVSWEVISKDGCDVLSRELVRAAELYESFNIIERCLTEMPPGPVLTEGFAYKPHTFALGYTEA
PRGENVHWLMTGNNQKLYRWRVRAATYNNWPALRYMFRGNTVSDAPLIVASIDPCYSCTERVTMVDVRKKKARTIEYKEL
ERYCRERKYSPLKF
>Mature_573_residues
GQGSESKRAQKYIKDLRSKFPGAILEESSQAPDQITVTVKLNDLPGIVEELYYRHNGWLSTMLGNDERELNGCFALYYVL
SMEGNSNKRENTWITVKALVPEKEPQFPSVTPRVPAAVWYEREVRDMFGLEPVGIPDARRLVLPDDWPDNLHPLRKDAMD
YRYRPEPVEENYNFIEVEGEGIVEVPLGPLHITSDEPGHFRLFVDGEYIVDADYRLFYVHRGMEKLAENRMDYDQITFLA
ERICGICGYAHSVAYAAAVETANGIEVPPRAQYIRTILLEVERLHSHLLNLGLAAHLVGFDSGFMHFFRVREKAMQMAEI
LTGGRKTYGINLIGGVRRDIFKEERDQVLRLIAEIRTELDELLDILINTPNFISRTQGVGRLERQVARDFSPVGPNMRGS
GYARDTRADHPYGAYDRVSWEVISKDGCDVLSRELVRAAELYESFNIIERCLTEMPPGPVLTEGFAYKPHTFALGYTEAP
RGENVHWLMTGNNQKLYRWRVRAATYNNWPALRYMFRGNTVSDAPLIVASIDPCYSCTERVTMVDVRKKKARTIEYKELE
RYCRERKYSPLKF

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=385, Percent_Identity=27.7922077922078, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI260898743, Length=375, Percent_Identity=28, Blast_Score=133, Evalue=5e-31,
Organism=Escherichia coli, GI1789076, Length=572, Percent_Identity=64.6853146853147, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1788832, Length=575, Percent_Identity=62.4347826086956, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=513, Percent_Identity=27.8752436647173, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17555284, Length=382, Percent_Identity=26.9633507853403, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17568379, Length=382, Percent_Identity=26.9633507853403, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI221459469, Length=383, Percent_Identity=27.9373368146214, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24638644, Length=381, Percent_Identity=28.8713910761155, Blast_Score=159, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 65990; Mature: 65859

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQGSESKRAQKYIKDLRSKFPGAILEESSQAPDQITVTVKLNDLPGIVEELYYRHNGWL
CCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCEE
STMLGNDERELNGCFALYYVLSMEGNSNKRENTWITVKALVPEKEPQFPSVTPRVPAAVW
EECCCCCCHHHHHHEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHH
YEREVRDMFGLEPVGIPDARRLVLPDDWPDNLHPLRKDAMDYRYRPEPVEENYNFIEVEG
HHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECC
EGIVEVPLGPLHITSDEPGHFRLFVDGEYIVDADYRLFYVHRGMEKLAENRMDYDQITFL
CCEEEECCCCEEECCCCCCEEEEEECCCEEEECCEEEEEEHHHHHHHHHCCCCHHHHHHH
AERICGICGYAHSVAYAAAVETANGIEVPPRAQYIRTILLEVERLHSHLLNLGLAAHLVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSGFMHFFRVREKAMQMAEILTGGRKTYGINLIGGVRRDIFKEERDQVLRLIAEIRTEL
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELLDILINTPNFISRTQGVGRLERQVARDFSPVGPNMRGSGYARDTRADHPYGAYDRVS
HHHHHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
WEVISKDGCDVLSRELVRAAELYESFNIIERCLTEMPPGPVLTEGFAYKPHTFALGYTEA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
PRGENVHWLMTGNNQKLYRWRVRAATYNNWPALRYMFRGNTVSDAPLIVASIDPCYSCTE
CCCCCEEEEEECCCCEEEEEEEEEEEECCCCEEEEEEECCCCCCCCEEEEECCHHHHHHH
RVTMVDVRKKKARTIEYKELERYCRERKYSPLKF
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GQGSESKRAQKYIKDLRSKFPGAILEESSQAPDQITVTVKLNDLPGIVEELYYRHNGWL
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCEE
STMLGNDERELNGCFALYYVLSMEGNSNKRENTWITVKALVPEKEPQFPSVTPRVPAAVW
EECCCCCCHHHHHHEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHH
YEREVRDMFGLEPVGIPDARRLVLPDDWPDNLHPLRKDAMDYRYRPEPVEENYNFIEVEG
HHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECC
EGIVEVPLGPLHITSDEPGHFRLFVDGEYIVDADYRLFYVHRGMEKLAENRMDYDQITFL
CCEEEECCCCEEECCCCCCEEEEEECCCEEEECCEEEEEEHHHHHHHHHCCCCHHHHHHH
AERICGICGYAHSVAYAAAVETANGIEVPPRAQYIRTILLEVERLHSHLLNLGLAAHLVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSGFMHFFRVREKAMQMAEILTGGRKTYGINLIGGVRRDIFKEERDQVLRLIAEIRTEL
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELLDILINTPNFISRTQGVGRLERQVARDFSPVGPNMRGSGYARDTRADHPYGAYDRVS
HHHHHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
WEVISKDGCDVLSRELVRAAELYESFNIIERCLTEMPPGPVLTEGFAYKPHTFALGYTEA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
PRGENVHWLMTGNNQKLYRWRVRAATYNNWPALRYMFRGNTVSDAPLIVASIDPCYSCTE
CCCCCEEEEEECCCCEEEEEEEEEEEECCCCEEEEEEECCCCCCCCEEEEECCHHHHHHH
RVTMVDVRKKKARTIEYKELERYCRERKYSPLKF
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]