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The map label for this gene is hycF [H]

Identifier: 83591008

GI number: 83591008

Start: 2282944

End: 2283498

Strand: Reverse

Name: hycF [H]

Synonym: Moth_2185

Alternate gene names: 83591008

Gene position: 2283498-2282944 (Counterclockwise)

Preceding gene: 83591009

Following gene: 83591007

Centisome position: 86.87

GC content: 54.59

Gene sequence:

>555_bases
ATGCTAAAACTGCTTAAAAAAGCACTGCAGGTCGGGGAAGCCACGGTTGAGTATCCCTTTAAGCCAGTAGAAGTGGCTCC
TGGCTTTCGCGGTAAACCGGAATATAACTTTGACAGGTGTATAGCGTGCGGTGCCTGTGCTACTGCCTGCCCGCCCAATG
CCATTACTATGGAGTATGATCTTAAGCAGGGCACGAAGACGTGGAATATCTTTTACGGTCGCTGTATTTTTTGCGGCCGC
TGTGAGGAAGTCTGTCCCACAGGGGCAATAGCCCTTACAACCGAGTTTGAACTGGCCGTTGCCAGCAAGGAAGACCTCTA
CTACCGGGCTGAAGTTCAACTTCGCAAGTGTAGCTGTTGTGGTGAATACTTTGCCCCTTCGCGCGAGCTGGAATATGTAA
TGTCTACCCTGGAGCAGTCAGGATTACCGGAAGGCGACCGGCAGGGCTGGAGGCAGCTTCTGGAAAAATGCCCGCAGTGC
CGGCGCCGGCAGGCGGCGGTGAGCGTAGCTGAAGCCACCGGTTCTAGGGACAGGGTGCAGGGAGGTGGACAATAG

Upstream 100 bases:

>100_bases
TTGAATACAAAGAACTGGAGCGTTACTGCCGGGAAAGAAAATACTCGCCGCTTAAATTTTAGCCTCGTTGATATACTGGC
GGAAGAAGGGGGATATATGA

Downstream 100 bases:

>100_bases
GATGGAACAGGAAGTAAAGCTGGCCGAGCTGAAGGCGAAGCTAAAAAATGTCATTAAACGCTCCGTCTACGTGTACCGGG
TGGATTGTGGCGGTTGCAAC

Product: formate hydrogenlyase complex iron-sulfur subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MLKLLKKALQVGEATVEYPFKPVEVAPGFRGKPEYNFDRCIACGACATACPPNAITMEYDLKQGTKTWNIFYGRCIFCGR
CEEVCPTGAIALTTEFELAVASKEDLYYRAEVQLRKCSCCGEYFAPSRELEYVMSTLEQSGLPEGDRQGWRQLLEKCPQC
RRRQAAVSVAEATGSRDRVQGGGQ

Sequences:

>Translated_184_residues
MLKLLKKALQVGEATVEYPFKPVEVAPGFRGKPEYNFDRCIACGACATACPPNAITMEYDLKQGTKTWNIFYGRCIFCGR
CEEVCPTGAIALTTEFELAVASKEDLYYRAEVQLRKCSCCGEYFAPSRELEYVMSTLEQSGLPEGDRQGWRQLLEKCPQC
RRRQAAVSVAEATGSRDRVQGGGQ
>Mature_184_residues
MLKLLKKALQVGEATVEYPFKPVEVAPGFRGKPEYNFDRCIACGACATACPPNAITMEYDLKQGTKTWNIFYGRCIFCGR
CEEVCPTGAIALTTEFELAVASKEDLYYRAEVQLRKCSCCGEYFAPSRELEYVMSTLEQSGLPEGDRQGWRQLLEKCPQC
RRRQAAVSVAEATGSRDRVQGGGQ

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=112, Percent_Identity=36.6071428571429, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789075, Length=167, Percent_Identity=44.311377245509, Blast_Score=157, Evalue=3e-40,
Organism=Escherichia coli, GI1788833, Length=169, Percent_Identity=50.2958579881657, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1788617, Length=109, Percent_Identity=40.3669724770642, Blast_Score=77, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17555194, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864306, Length=110, Percent_Identity=37.2727272727273, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20477; Mature: 20477

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLLKKALQVGEATVEYPFKPVEVAPGFRGKPEYNFDRCIACGACATACPPNAITMEYD
CHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCHHCCCCCCCEEEEEE
LKQGTKTWNIFYGRCIFCGRCEEVCPTGAIALTTEFELAVASKEDLYYRAEVQLRKCSCC
CCCCCEEEEEEEEEEEEECCCHHHCCCCEEEEEEEEEEEEECCCCEEEEEHHHHHHHHHH
GEYFAPSRELEYVMSTLEQSGLPEGDRQGWRQLLEKCPQCRRRQAAVSVAEATGSRDRVQ
HHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGGQ
CCCC
>Mature Secondary Structure
MLKLLKKALQVGEATVEYPFKPVEVAPGFRGKPEYNFDRCIACGACATACPPNAITMEYD
CHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCHHCCCCCCCEEEEEE
LKQGTKTWNIFYGRCIFCGRCEEVCPTGAIALTTEFELAVASKEDLYYRAEVQLRKCSCC
CCCCCEEEEEEEEEEEEECCCHHHCCCCEEEEEEEEEEEEECCCCEEEEEHHHHHHHHHH
GEYFAPSRELEYVMSTLEQSGLPEGDRQGWRQLLEKCPQCRRRQAAVSVAEATGSRDRVQ
HHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGGQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]