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The map label for this gene is hycG [H]

Identifier: 83591007

GI number: 83591007

Start: 2282184

End: 2282942

Strand: Reverse

Name: hycG [H]

Synonym: Moth_2184

Alternate gene names: 83591007

Gene position: 2282942-2282184 (Counterclockwise)

Preceding gene: 83591008

Following gene: 83591006

Centisome position: 86.84

GC content: 53.36

Gene sequence:

>759_bases
ATGGAACAGGAAGTAAAGCTGGCCGAGCTGAAGGCGAAGCTAAAAAATGTCATTAAACGCTCCGTCTACGTGTACCGGGT
GGATTGTGGCGGTTGCAACGGGTGCGAGATTGAAATTTTCGCCACTATCTCACCTGTTTATGACCCGGAAAGGCTGGGGT
TCAAGGTAGTAGCCTCCCCACGGCATGCCGATATCCTCCTTTTTACCGGCCCCATGACCAGGGCCATGCGTTTGCCGGCC
ATTCGGGCGTATCAGGCGGCACCTGATCCCAAGATAGTAGTGGCTTACGGAACGTGTGGCTGCAGCGGCGGTATCTTTCA
CGATTGTTACTCTGTATGGGGTGGGACCGGGAGTATCTTTCCTGTGGATGTATATATCCCCGGCTGTCCTCCAACTCCCG
GGGCAACGATCTACGGTTTGGGCCTGGCCCTGGATCTACTTCAACAAAAAATGAAGGGTGAACAATACACAGAAAAGGAG
GTGGCCCGGATCACGTCGCAATATGCGGGAGTTTCTCCCGACCTGGCCAGAGATATCGAGAGGGAGGCCCGTCGTTTAAG
CGGTTACCTCCATGGCCGGCGTATAACCGAGAAATACCTCCAGTTCGTACGCGAGGATCCCCTGGCGGTCGACCAGAAAA
TTAAAGAGCTGGTAAAGCAGGAGAAGGATTCACGGCTGGCTGAAGTTTATTTGAGCCTGCATAAAATTTATCTGGACTAT
TTAGGTAAGACGGAGAAGGCGGGCCATCATGCCGGGTAG

Upstream 100 bases:

>100_bases
CTTCTGGAAAAATGCCCGCAGTGCCGGCGCCGGCAGGCGGCGGTGAGCGTAGCTGAAGCCACCGGTTCTAGGGACAGGGT
GCAGGGAGGTGGACAATAGG

Downstream 100 bases:

>100_bases
GGTCGAATTTTATAAGCTATGTAAGAGATTTGTGGATGAAAAGGACGTTCCGGCTGAGGTTCAGCAGCTGGAGTACTATG
CCCTGGCTATTGGCCACCAT

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MEQEVKLAELKAKLKNVIKRSVYVYRVDCGGCNGCEIEIFATISPVYDPERLGFKVVASPRHADILLFTGPMTRAMRLPA
IRAYQAAPDPKIVVAYGTCGCSGGIFHDCYSVWGGTGSIFPVDVYIPGCPPTPGATIYGLGLALDLLQQKMKGEQYTEKE
VARITSQYAGVSPDLARDIEREARRLSGYLHGRRITEKYLQFVREDPLAVDQKIKELVKQEKDSRLAEVYLSLHKIYLDY
LGKTEKAGHHAG

Sequences:

>Translated_252_residues
MEQEVKLAELKAKLKNVIKRSVYVYRVDCGGCNGCEIEIFATISPVYDPERLGFKVVASPRHADILLFTGPMTRAMRLPA
IRAYQAAPDPKIVVAYGTCGCSGGIFHDCYSVWGGTGSIFPVDVYIPGCPPTPGATIYGLGLALDLLQQKMKGEQYTEKE
VARITSQYAGVSPDLARDIEREARRLSGYLHGRRITEKYLQFVREDPLAVDQKIKELVKQEKDSRLAEVYLSLHKIYLDY
LGKTEKAGHHAG
>Mature_252_residues
MEQEVKLAELKAKLKNVIKRSVYVYRVDCGGCNGCEIEIFATISPVYDPERLGFKVVASPRHADILLFTGPMTRAMRLPA
IRAYQAAPDPKIVVAYGTCGCSGGIFHDCYSVWGGTGSIFPVDVYIPGCPPTPGATIYGLGLALDLLQQKMKGEQYTEKE
VARITSQYAGVSPDLARDIEREARRLSGYLHGRRITEKYLQFVREDPLAVDQKIKELVKQEKDSRLAEVYLSLHKIYLDY
LGKTEKAGHHAG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=126, Percent_Identity=42.0634920634921, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1789074, Length=236, Percent_Identity=54.2372881355932, Blast_Score=263, Evalue=6e-72,
Organism=Escherichia coli, GI1788834, Length=240, Percent_Identity=52.0833333333333, Blast_Score=262, Evalue=1e-71,
Organism=Escherichia coli, GI1788624, Length=160, Percent_Identity=36.875, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=122, Percent_Identity=39.344262295082, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI18859983, Length=126, Percent_Identity=41.2698412698413, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24642371, Length=126, Percent_Identity=41.2698412698413, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24651058, Length=111, Percent_Identity=43.2432432432432, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 28015; Mature: 28015

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQEVKLAELKAKLKNVIKRSVYVYRVDCGGCNGCEIEIFATISPVYDPERLGFKVVASP
CCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEEECCCCCHHHCCEEEEECC
RHADILLFTGPMTRAMRLPAIRAYQAAPDPKIVVAYGTCGCSGGIFHDCYSVWGGTGSIF
CCCEEEEEECCHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEE
PVDVYIPGCPPTPGATIYGLGLALDLLQQKMKGEQYTEKEVARITSQYAGVSPDLARDIE
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
REARRLSGYLHGRRITEKYLQFVREDPLAVDQKIKELVKQEKDSRLAEVYLSLHKIYLDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGKTEKAGHHAG
HCCCCCCCCCCC
>Mature Secondary Structure
MEQEVKLAELKAKLKNVIKRSVYVYRVDCGGCNGCEIEIFATISPVYDPERLGFKVVASP
CCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEEECCCCCHHHCCEEEEECC
RHADILLFTGPMTRAMRLPAIRAYQAAPDPKIVVAYGTCGCSGGIFHDCYSVWGGTGSIF
CCCEEEEEECCHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEE
PVDVYIPGCPPTPGATIYGLGLALDLLQQKMKGEQYTEKEVARITSQYAGVSPDLARDIE
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
REARRLSGYLHGRRITEKYLQFVREDPLAVDQKIKELVKQEKDSRLAEVYLSLHKIYLDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGKTEKAGHHAG
HCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]