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The map label for this gene is hycI

Identifier: 83591005

GI number: 83591005

Start: 2281324

End: 2281800

Strand: Reverse

Name: hycI

Synonym: Moth_2182

Alternate gene names: 83591005

Gene position: 2281800-2281324 (Counterclockwise)

Preceding gene: 83591006

Following gene: 83591004

Centisome position: 86.8

GC content: 52.41

Gene sequence:

>477_bases
GTGAAGGGGCTAGTGATTACTGTAGGCAACGAACTGATGGGCGATGATGGAGCCGGGCCGTTGCTGGCCCGGCTCTTGGA
ACAAAAACGCATTCCCGGTTGGGAGGTAATTAACGGCGGGCCGGCGCCGGAAAATTACCTGCACCAGGTACGGGATCTCA
AACCGGGAGCGGTTGTGGTAGTGGATGCCTGCGAAATGAATCTGCAGGCCGGGAGCATACGCCTCATATCTGAGAAGGAT
ATTGCCTGTGAGTTTTTCCTGACCACCCACCGGTTACCCCTGTCATTTTTCATCTCGGCTTTGAAAGAATTGGTGCCGGA
AGTCTATTTTATAGGTATCCAGCCGGCGGTAGTCGCCTTCGGTTTGCCGGTTTCGCCTGAAGTAAAACAAGCCGTTGAGA
CTGTATATCAAAAGCTGCAGGCCGGGAAGCTCGATTTTCAATGTCTTGGACAGTTAGGAGAGAAAGAAGATGCATGA

Upstream 100 bases:

>100_bases
GCCAGTTTTCTCCCGGAAGAAAAACAATGGACCGACTGCCTGCTCCGGCTTCTTTATGCCATGGAGAAAGAACCGGCTAT
ATATCTGGTGGTGAGGCTGA

Downstream 100 bases:

>100_bases
GCTGGCCCTGACGGCGGAGTTACTCAGGTTGTTAGAGAACAATGCGCGCGAGAAAGGTATAAAGCATATCAAGAGAGTAA
AGCTGGTGATAGGAGCATTG

Product: hydrogenase 3 maturation protease

Products: NA

Alternate protein names: HycI protease [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKGLVITVGNELMGDDGAGPLLARLLEQKRIPGWEVINGGPAPENYLHQVRDLKPGAVVVVDACEMNLQAGSIRLISEKD
IACEFFLTTHRLPLSFFISALKELVPEVYFIGIQPAVVAFGLPVSPEVKQAVETVYQKLQAGKLDFQCLGQLGEKEDA

Sequences:

>Translated_158_residues
MKGLVITVGNELMGDDGAGPLLARLLEQKRIPGWEVINGGPAPENYLHQVRDLKPGAVVVVDACEMNLQAGSIRLISEKD
IACEFFLTTHRLPLSFFISALKELVPEVYFIGIQPAVVAFGLPVSPEVKQAVETVYQKLQAGKLDFQCLGQLGEKEDA
>Mature_158_residues
MKGLVITVGNELMGDDGAGPLLARLLEQKRIPGWEVINGGPAPENYLHQVRDLKPGAVVVVDACEMNLQAGSIRLISEKD
IACEFFLTTHRLPLSFFISALKELVPEVYFIGIQPAVVAFGLPVSPEVKQAVETVYQKLQAGKLDFQCLGQLGEKEDA

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3 [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=141, Percent_Identity=48.2269503546099, Blast_Score=140, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: =3.4.23.51 [H]

Molecular weight: Translated: 17188; Mature: 17188

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLVITVGNELMGDDGAGPLLARLLEQKRIPGWEVINGGPAPENYLHQVRDLKPGAVVV
CCCEEEEECCHHCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCEEEE
VDACEMNLQAGSIRLISEKDIACEFFLTTHRLPLSFFISALKELVPEVYFIGIQPAVVAF
EECCCCCCCCCCEEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHEEECCCHHHHHC
GLPVSPEVKQAVETVYQKLQAGKLDFQCLGQLGEKEDA
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MKGLVITVGNELMGDDGAGPLLARLLEQKRIPGWEVINGGPAPENYLHQVRDLKPGAVVV
CCCEEEEECCHHCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCEEEE
VDACEMNLQAGSIRLISEKDIACEFFLTTHRLPLSFFISALKELVPEVYFIGIQPAVVAF
EECCCCCCCCCCEEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHEEECCCHHHHHC
GLPVSPEVKQAVETVYQKLQAGKLDFQCLGQLGEKEDA
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]