Click here to switch to the map view.

The map label for this gene is yeaJ [H]

Identifier: 83590974

GI number: 83590974

Start: 2253524

End: 2254351

Strand: Reverse

Name: yeaJ [H]

Synonym: Moth_2151

Alternate gene names: 83590974

Gene position: 2254351-2253524 (Counterclockwise)

Preceding gene: 83590975

Following gene: 83590973

Centisome position: 85.76

GC content: 54.59

Gene sequence:

>828_bases
ATGAGCGTCGAAAGGGTCTATCTCATCGACTGGCTGCGAGTGGCCTGCAAAGGGAGTAAGTTTTTTATTTACCTCCTAGT
ACTCACCTGGCTGGTATATTTTATCCTTACCTCTCAACTGAGAGCTAGAACGAGAACGGCCGCGATACTGCTTTTTATCG
GCCTGCCCTTGGTGCTGGCCGGCATTTCCCTGGACCTTCTGGGAGAGTTTTTTCACCTCTCTACATTGCTCAAAAGAATC
GTGGGCGAACTTATTCTTTCCAACACCGGTACCTTTTTAGTCCTATATTCGATGATGTTGATGATCATGGAACTGGTCCA
GGCTTCATGCCGTTACCAGCGCGAGGCCGAAAGCGACCCCCTTACCGGCCTATACAATCGGCGGACCTTTTTTGCCTCGG
CGGAGAGCGTGCTAAAGGAGGCCCGGGAAGGGAAGCGCAGCCCGATGTTGGCTATTTTAGACCTGGATAACATGAAAAAA
ATCAACGACACCTGGGGACATCAGTATGGGGATGAAGCCCTGAAACGGGTAGCTCAGGCCATCCAAAAAAGCGTTCAGGA
AGGGGATGTAACGGCCCGCTATGGGGGCGACGAATTTGTCATCCTCTTCCCAGGCAAAGGGCCGCGCCTGGAAACACTCC
GGGCCCGACTTAAGAAGCATCTGCAGGCGCTACGGTTGTCCGGTGCGGAGATACCCTTGAGCCTGTCGGTGGGGCTGGCG
CGGTTCCCGGCAGACGGGAAAGACGTAGATACGCTGCTCGCAGTGGCCGACGCCAGGATGTATGCGGACAAGGAGGGAAA
GAAAGGCCGCCGAAGGGCCGGCATTTAG

Upstream 100 bases:

>100_bases
TGGAAAAATATGTGCTGAAACGGCAATGGGGGAGTTTAAAAAGCCTTAACCGTCTTTAAAGCGACTGTATAGCGATAAAA
TTTTCTGCTGGGGAAAGGGA

Downstream 100 bases:

>100_bases
CAGTCCTGCGTGATTGCCGGTCCGGCAAGCAGGCCTTTTCTGGCAACCACTCCCAGCAGGAGGTATGTGGGTCCTTTCAT
GGAAAGAGGAAAATTAATGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSVERVYLIDWLRVACKGSKFFIYLLVLTWLVYFILTSQLRARTRTAAILLFIGLPLVLAGISLDLLGEFFHLSTLLKRI
VGELILSNTGTFLVLYSMMLMIMELVQASCRYQREAESDPLTGLYNRRTFFASAESVLKEAREGKRSPMLAILDLDNMKK
INDTWGHQYGDEALKRVAQAIQKSVQEGDVTARYGGDEFVILFPGKGPRLETLRARLKKHLQALRLSGAEIPLSLSVGLA
RFPADGKDVDTLLAVADARMYADKEGKKGRRRAGI

Sequences:

>Translated_275_residues
MSVERVYLIDWLRVACKGSKFFIYLLVLTWLVYFILTSQLRARTRTAAILLFIGLPLVLAGISLDLLGEFFHLSTLLKRI
VGELILSNTGTFLVLYSMMLMIMELVQASCRYQREAESDPLTGLYNRRTFFASAESVLKEAREGKRSPMLAILDLDNMKK
INDTWGHQYGDEALKRVAQAIQKSVQEGDVTARYGGDEFVILFPGKGPRLETLRARLKKHLQALRLSGAEIPLSLSVGLA
RFPADGKDVDTLLAVADARMYADKEGKKGRRRAGI
>Mature_274_residues
SVERVYLIDWLRVACKGSKFFIYLLVLTWLVYFILTSQLRARTRTAAILLFIGLPLVLAGISLDLLGEFFHLSTLLKRIV
GELILSNTGTFLVLYSMMLMIMELVQASCRYQREAESDPLTGLYNRRTFFASAESVLKEAREGKRSPMLAILDLDNMKKI
NDTWGHQYGDEALKRVAQAIQKSVQEGDVTARYGGDEFVILFPGKGPRLETLRARLKKHLQALRLSGAEIPLSLSVGLAR
FPADGKDVDTLLAVADARMYADKEGKKGRRRAGI

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081974, Length=184, Percent_Identity=35.3260869565217, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI145693134, Length=184, Percent_Identity=36.9565217391304, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786584, Length=175, Percent_Identity=34.2857142857143, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787262, Length=180, Percent_Identity=31.1111111111111, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=35.672514619883, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081977, Length=172, Percent_Identity=31.9767441860465, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082007, Length=178, Percent_Identity=32.5842696629214, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788085, Length=178, Percent_Identity=28.6516853932584, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87081881, Length=184, Percent_Identity=29.8913043478261, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=31.0975609756098, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=36.5384615384615, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788381, Length=189, Percent_Identity=29.1005291005291, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=32.9192546583851, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788956, Length=159, Percent_Identity=30.188679245283, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 30876; Mature: 30745

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVERVYLIDWLRVACKGSKFFIYLLVLTWLVYFILTSQLRARTRTAAILLFIGLPLVLA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GISLDLLGEFFHLSTLLKRIVGELILSNTGTFLVLYSMMLMIMELVQASCRYQREAESDP
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LTGLYNRRTFFASAESVLKEAREGKRSPMLAILDLDNMKKINDTWGHQYGDEALKRVAQA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHH
IQKSVQEGDVTARYGGDEFVILFPGKGPRLETLRARLKKHLQALRLSGAEIPLSLSVGLA
HHHHHHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
RFPADGKDVDTLLAVADARMYADKEGKKGRRRAGI
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
SVERVYLIDWLRVACKGSKFFIYLLVLTWLVYFILTSQLRARTRTAAILLFIGLPLVLA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GISLDLLGEFFHLSTLLKRIVGELILSNTGTFLVLYSMMLMIMELVQASCRYQREAESDP
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LTGLYNRRTFFASAESVLKEAREGKRSPMLAILDLDNMKKINDTWGHQYGDEALKRVAQA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHH
IQKSVQEGDVTARYGGDEFVILFPGKGPRLETLRARLKKHLQALRLSGAEIPLSLSVGLA
HHHHHHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHH
RFPADGKDVDTLLAVADARMYADKEGKKGRRRAGI
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]