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The map label for this gene is purB [H]

Identifier: 83590882

GI number: 83590882

Start: 2143773

End: 2145068

Strand: Reverse

Name: purB [H]

Synonym: Moth_2052

Alternate gene names: 83590882

Gene position: 2145068-2143773 (Counterclockwise)

Preceding gene: 83590883

Following gene: 83590881

Centisome position: 81.6

GC content: 59.65

Gene sequence:

>1296_bases
ATGATCGAGCGCTATACCCTGCCGGAAATGGGTAAAATTTGGGAACCGGAGCACAAGTTCCGCACCTGGCTGGCCATTGA
GATCTATGCCTGCGAGGCCTGGGCGGAGCTCGGCCGGATTCCGCCGGCCGCCCTGGAAGAGATCAAGGCCAGGGCCGATT
TCGACATTGACCGGATCAATGAAATTGAAGCCACCACCCGCCATGACGTCCTGGCTTTCCTCACTGCCGTTGCGGAGAAG
GTCGGTGATGCCTCCAAGTATATTCATCTTGGTATGACATCCTCAGACGTCCTGGATACGGCCCTGGCCGTACAGATGCG
GGACGCCGCCGACCTGCTCCTTAAGCGCCTCCGGGATCTGCGGGCCGAACTGGTGAAAAAGGCCATGGAGCATAAATATA
CTTTGATGATCGGCCGCACCCACGGCGTCCACGCCGAGCCCACCACTTTTGGCCTCAAGATGGCCCTGTGGGTTATGGAG
GTTGATCGGCACCTCATCCGGCTGGAACAGGCTAAAGAGATGATCAGCGTCGGCAAAATCTCCGGCGCCGTGGGTACTTT
CGCCAATATCAACCCCCGGGTGGAGGAGCACGTCTGCCGTCGCCTGGGGCTCAAGCCCGCCAGCGTCTCCACCCAGATAA
TCCAACGGGACCGTCACGCCCAGTTCCTGGCCACCCTGGCCATCATCGGCAGCTCCCTGGAGAAAATGGCCACAGAGATC
CGCAACCTCCAGCGCACCGACATCCTGGAGGTGGAGGAGCCCTTCGCTAAAGGTCAAAAAGGGTCTTCGGCTATGCCCCA
CAAACGAAACCCCATTATATCGGAACGGGTGGCCGGCCTTTCGCGAGTGTTGCGGGGCAACGCCCTGGCCGCCATGGAGG
ACATCGCCCTCTGGCACGAGCGGGATCTCACCCATTCCTCCGTTGAGCGGGTGATTATCCCCGACAGCACCATCCTGCTG
GACTATATGCTGGTAAAGTTTACCGGGATTATCGCCGGCCTTAACGTCTACCCGGAGAACATGCGCCGGAATCTCGAGGC
CACCCACGGCCTGGTCTTTTCCCAGCGGGTCCTCCTGGCCCTGGTGAATAAGGGCGTCCTGCGGGAAACGGCCTACGCCT
GGGTGCAGCGCAACGCCCTCAAGGCCTGGCAGACCCGCCAGCCATTTAAAGAACTGGTCCTTAAGGACCAGGATATCATG
TCTCGCCTGGATCCGAAGGAAGTAGAGGCCCTCTTTGATTACGACTACCACCTGCGCCACGTAGATTATATCTTCCGCCG
CGCCGGTTTGGAGTAG

Upstream 100 bases:

>100_bases
GGCTGGCGGCCTATAAGCAGGAACTGGCCCAGGAAGTGGCGGTCAAAGATGCCCGGCTGGCGCAACTGGGTATTGAAGGT
TATCTGGGGGAGGAGAAAAA

Downstream 100 bases:

>100_bases
CGGCGGGCGGACAACGTCCGGACCGATGCAAGGGGCTGGGGGTTACAGTCGAAGGTCGACTCGGTTTAAAACATCTGATA
CTTCCGCGAAGCCGAAAGCT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGKIWEPEHKFRTWLAIEIYACEAWAELGRIPPAALEEIKARADFDIDRINEIEATTRHDVLAFLTAVAEK
VGDASKYIHLGMTSSDVLDTALAVQMRDAADLLLKRLRDLRAELVKKAMEHKYTLMIGRTHGVHAEPTTFGLKMALWVME
VDRHLIRLEQAKEMISVGKISGAVGTFANINPRVEEHVCRRLGLKPASVSTQIIQRDRHAQFLATLAIIGSSLEKMATEI
RNLQRTDILEVEEPFAKGQKGSSAMPHKRNPIISERVAGLSRVLRGNALAAMEDIALWHERDLTHSSVERVIIPDSTILL
DYMLVKFTGIIAGLNVYPENMRRNLEATHGLVFSQRVLLALVNKGVLRETAYAWVQRNALKAWQTRQPFKELVLKDQDIM
SRLDPKEVEALFDYDYHLRHVDYIFRRAGLE

Sequences:

>Translated_431_residues
MIERYTLPEMGKIWEPEHKFRTWLAIEIYACEAWAELGRIPPAALEEIKARADFDIDRINEIEATTRHDVLAFLTAVAEK
VGDASKYIHLGMTSSDVLDTALAVQMRDAADLLLKRLRDLRAELVKKAMEHKYTLMIGRTHGVHAEPTTFGLKMALWVME
VDRHLIRLEQAKEMISVGKISGAVGTFANINPRVEEHVCRRLGLKPASVSTQIIQRDRHAQFLATLAIIGSSLEKMATEI
RNLQRTDILEVEEPFAKGQKGSSAMPHKRNPIISERVAGLSRVLRGNALAAMEDIALWHERDLTHSSVERVIIPDSTILL
DYMLVKFTGIIAGLNVYPENMRRNLEATHGLVFSQRVLLALVNKGVLRETAYAWVQRNALKAWQTRQPFKELVLKDQDIM
SRLDPKEVEALFDYDYHLRHVDYIFRRAGLE
>Mature_431_residues
MIERYTLPEMGKIWEPEHKFRTWLAIEIYACEAWAELGRIPPAALEEIKARADFDIDRINEIEATTRHDVLAFLTAVAEK
VGDASKYIHLGMTSSDVLDTALAVQMRDAADLLLKRLRDLRAELVKKAMEHKYTLMIGRTHGVHAEPTTFGLKMALWVME
VDRHLIRLEQAKEMISVGKISGAVGTFANINPRVEEHVCRRLGLKPASVSTQIIQRDRHAQFLATLAIIGSSLEKMATEI
RNLQRTDILEVEEPFAKGQKGSSAMPHKRNPIISERVAGLSRVLRGNALAAMEDIALWHERDLTHSSVERVIIPDSTILL
DYMLVKFTGIIAGLNVYPENMRRNLEATHGLVFSQRVLLALVNKGVLRETAYAWVQRNALKAWQTRQPFKELVLKDQDIM
SRLDPKEVEALFDYDYHLRHVDYIFRRAGLE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=386, Percent_Identity=31.0880829015544, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=31.331592689295, Blast_Score=168, Evalue=8e-42,
Organism=Escherichia coli, GI1787376, Length=299, Percent_Identity=30.1003344481605, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790398, Length=398, Percent_Identity=22.8643216080402, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=389, Percent_Identity=32.133676092545, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI32564234, Length=330, Percent_Identity=33.030303030303, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553882, Length=277, Percent_Identity=27.0758122743682, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI32565146, Length=274, Percent_Identity=27.3722627737226, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=383, Percent_Identity=32.8981723237598, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24647570, Length=383, Percent_Identity=30.0261096605744, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49086; Mature: 49086

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGKIWEPEHKFRTWLAIEIYACEAWAELGRIPPAALEEIKARADFDIDRIN
CCCCCCCCCCCCCCCCCHHHHEEEEEEEEEHHHHHHHCCCCHHHHHHHHHHCCCCHHHHH
EIEATTRHDVLAFLTAVAEKVGDASKYIHLGMTSSDVLDTALAVQMRDAADLLLKRLRDL
HHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RAELVKKAMEHKYTLMIGRTHGVHAEPTTFGLKMALWVMEVDRHLIRLEQAKEMISVGKI
HHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANINPRVEEHVCRRLGLKPASVSTQIIQRDRHAQFLATLAIIGSSLEKMATEI
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDILEVEEPFAKGQKGSSAMPHKRNPIISERVAGLSRVLRGNALAAMEDIALWHE
HHCHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RDLTHSSVERVIIPDSTILLDYMLVKFTGIIAGLNVYPENMRRNLEATHGLVFSQRVLLA
CCCCHHHCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVNKGVLRETAYAWVQRNALKAWQTRQPFKELVLKDQDIMSRLDPKEVEALFDYDYHLRH
HHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCHHHHHHHHCCCHHHHH
VDYIFRRAGLE
HHHHHHHCCCC
>Mature Secondary Structure
MIERYTLPEMGKIWEPEHKFRTWLAIEIYACEAWAELGRIPPAALEEIKARADFDIDRIN
CCCCCCCCCCCCCCCCCHHHHEEEEEEEEEHHHHHHHCCCCHHHHHHHHHHCCCCHHHHH
EIEATTRHDVLAFLTAVAEKVGDASKYIHLGMTSSDVLDTALAVQMRDAADLLLKRLRDL
HHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RAELVKKAMEHKYTLMIGRTHGVHAEPTTFGLKMALWVMEVDRHLIRLEQAKEMISVGKI
HHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANINPRVEEHVCRRLGLKPASVSTQIIQRDRHAQFLATLAIIGSSLEKMATEI
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDILEVEEPFAKGQKGSSAMPHKRNPIISERVAGLSRVLRGNALAAMEDIALWHE
HHCHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RDLTHSSVERVIIPDSTILLDYMLVKFTGIIAGLNVYPENMRRNLEATHGLVFSQRVLLA
CCCCHHHCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVNKGVLRETAYAWVQRNALKAWQTRQPFKELVLKDQDIMSRLDPKEVEALFDYDYHLRH
HHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHCCCHHHHHHHHCCCHHHHH
VDYIFRRAGLE
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]