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The map label for this gene is hisZ

Identifier: 83590867

GI number: 83590867

Start: 2126561

End: 2127730

Strand: Reverse

Name: hisZ

Synonym: Moth_2037

Alternate gene names: 83590867

Gene position: 2127730-2126561 (Counterclockwise)

Preceding gene: 83590868

Following gene: 83590866

Centisome position: 80.94

GC content: 66.58

Gene sequence:

>1170_bases
TTGGCCAGCAACCTTCCCCTCCAGCTACCGGCCGGGGTAAGTGATCTTCTACCGCCGGAGGCGGCCGCCCTCCGGCAGCT
GGAGCAGCGACTGTTAAATTGTTTTCGCAGCTGGGGTTACCAGGAAGTAATGACCCCGACCTTTGAATTCGCCACTACCT
TTCAGGCCGGCTCCCCGGCCGGGGAAGAAGGGGCCCTCTACAAGTTTATCGACCGCCAGGGGCGGGTCCTGGCCCTACGG
CCGGAAATGACCGCCCCCATTGCCCGCCTGGTGGCAACCTCCCTGCGGCGCCGGGAACTACCCCTGCGCCTGGGGTATAG
CGCCCGGGTCTTCCGCTATGAGGAGCCCCAGGCCGGCCGCCGGCGGGAGTTTCACCAGGCCGGCGTCGAACTTATTGGCG
CCGGGGGAGTGGCCGGAGATGTCGAGATTATCGCCCTGGCGGTGGAGAGTCTGGCGCAGGCCGGTCTGGAGGATTTCCGG
CTGGGCCTGGGCCAGGTGGCCGTGACCAAAGGCGTTCTCCAGGATCTGGCTCTGCCGCCCGAAGCGGTGGCCGGTATCAA
ATCCGCCCTGGCCAGCAAGGACCTGGTAGCCCTGGAACGGATCTATGATGAGTACCATCTGGAAGGTGAACGCCGGCGGC
GCTTGGAGCTGCTGGCCACCATCCACGGTGGCCGGGAAGCCCTGGAGGAAGCCCGGGCTTGTTTCGGCCGGACGGCTGCG
GCCGCTTCCCTGGCAGAGTTGTCCCGGGTCTGGGAGGCCCTGGGGGCCGCCGGCCTGGAGAAGTGGCTTTTTATCGACCT
TGGTATTCTGCGGGACTTTGATTATTATACGGGCATTGTCTTTGAAGGTTATGTGCCGGGCCTGGGAGCCCCGGTTTGCG
GCGGCGGCCGTTATGACGGCCTGCTGGCCCAGTTCGGTTATCCCTGCCCGGCTACGGGTTTTGCCCTGGGCCTGGAGCGG
TTGCTCCTGGCCCGGGGAGAGACGGCACCGGCCTCGCTTGCGGGAGGCTACCTGGTGGCCGGGCGGGACCTGGCTGCCCT
CCTGAAAAGGGCGCGGGAATTGCGCAGCAAAGGAACGGCGGTAGTTCTCGACGGCGAGAGCCGGAGCCGCCAGGAGGCGG
CAGCCCGGGCCGCCGCCCGCGGCTTGAACCTGGAATGGATCGGGGAGTAA

Upstream 100 bases:

>100_bases
AGTGAGGGCTGTGGATGGCGGCAACGGGCCTTTGCCCGGAGGGACGGCTTGTGCTATTATTACGTTAATACGTTAACATA
GTAAAGTAGGTGCAAACGGG

Downstream 100 bases:

>100_bases
GTATTTTTGGAGGAGCCCTTCATGGTGGCTGACGTCCCCGCCAGCGAACCATAAAATGTAGGCTGAGGCAGAGGGCTGGA
GGGTACAGGCGGAGGGCGAC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MASNLPLQLPAGVSDLLPPEAAALRQLEQRLLNCFRSWGYQEVMTPTFEFATTFQAGSPAGEEGALYKFIDRQGRVLALR
PEMTAPIARLVATSLRRRELPLRLGYSARVFRYEEPQAGRRREFHQAGVELIGAGGVAGDVEIIALAVESLAQAGLEDFR
LGLGQVAVTKGVLQDLALPPEAVAGIKSALASKDLVALERIYDEYHLEGERRRRLELLATIHGGREALEEARACFGRTAA
AASLAELSRVWEALGAAGLEKWLFIDLGILRDFDYYTGIVFEGYVPGLGAPVCGGGRYDGLLAQFGYPCPATGFALGLER
LLLARGETAPASLAGGYLVAGRDLAALLKRARELRSKGTAVVLDGESRSRQEAAARAAARGLNLEWIGE

Sequences:

>Translated_389_residues
MASNLPLQLPAGVSDLLPPEAAALRQLEQRLLNCFRSWGYQEVMTPTFEFATTFQAGSPAGEEGALYKFIDRQGRVLALR
PEMTAPIARLVATSLRRRELPLRLGYSARVFRYEEPQAGRRREFHQAGVELIGAGGVAGDVEIIALAVESLAQAGLEDFR
LGLGQVAVTKGVLQDLALPPEAVAGIKSALASKDLVALERIYDEYHLEGERRRRLELLATIHGGREALEEARACFGRTAA
AASLAELSRVWEALGAAGLEKWLFIDLGILRDFDYYTGIVFEGYVPGLGAPVCGGGRYDGLLAQFGYPCPATGFALGLER
LLLARGETAPASLAGGYLVAGRDLAALLKRARELRSKGTAVVLDGESRSRQEAAARAAARGLNLEWIGE
>Mature_388_residues
ASNLPLQLPAGVSDLLPPEAAALRQLEQRLLNCFRSWGYQEVMTPTFEFATTFQAGSPAGEEGALYKFIDRQGRVLALRP
EMTAPIARLVATSLRRRELPLRLGYSARVFRYEEPQAGRRREFHQAGVELIGAGGVAGDVEIIALAVESLAQAGLEDFRL
GLGQVAVTKGVLQDLALPPEAVAGIKSALASKDLVALERIYDEYHLEGERRRRLELLATIHGGREALEEARACFGRTAAA
ASLAELSRVWEALGAAGLEKWLFIDLGILRDFDYYTGIVFEGYVPGLGAPVCGGGRYDGLLAQFGYPCPATGFALGLERL
LLARGETAPASLAGGYLVAGRDLAALLKRARELRSKGTAVVLDGESRSRQEAAARAAARGLNLEWIGE

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI15029520, Length=344, Percent_Identity=26.453488372093, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI6996014, Length=344, Percent_Identity=27.3255813953488, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1788861, Length=336, Percent_Identity=34.2261904761905, Blast_Score=124, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71993686, Length=360, Percent_Identity=25.8333333333333, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71993693, Length=360, Percent_Identity=25.8333333333333, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6325290, Length=365, Percent_Identity=24.3835616438356, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24643061, Length=360, Percent_Identity=25, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24643059, Length=374, Percent_Identity=24.0641711229947, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_MOOTA (Q2RGV6)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430876.1
- ProteinModelPortal:   Q2RGV6
- SMR:   Q2RGV6
- STRING:   Q2RGV6
- GeneID:   3831412
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2037
- NMPDR:   fig|264732.9.peg.2058
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   YREHVKS
- PhylomeDB:   Q2RGV6
- BioCyc:   MTHE264732:MOTH_2037-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 41905; Mature: 41773

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNLPLQLPAGVSDLLPPEAAALRQLEQRLLNCFRSWGYQEVMTPTFEFATTFQAGSPA
CCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCC
GEEGALYKFIDRQGRVLALRPEMTAPIARLVATSLRRRELPLRLGYSARVFRYEEPQAGR
CCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCHH
RREFHQAGVELIGAGGVAGDVEIIALAVESLAQAGLEDFRLGLGQVAVTKGVLQDLALPP
HHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCH
EAVAGIKSALASKDLVALERIYDEYHLEGERRRRLELLATIHGGREALEEARACFGRTAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AASLAELSRVWEALGAAGLEKWLFIDLGILRDFDYYTGIVFEGYVPGLGAPVCGGGRYDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEECCCCCCCCCCCCCCCCCC
LLAQFGYPCPATGFALGLERLLLARGETAPASLAGGYLVAGRDLAALLKRARELRSKGTA
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECHHHHHHHHHHHHHHCCCCE
VVLDGESRSRQEAAARAAARGLNLEWIGE
EEEECCCCHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
ASNLPLQLPAGVSDLLPPEAAALRQLEQRLLNCFRSWGYQEVMTPTFEFATTFQAGSPA
CCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCC
GEEGALYKFIDRQGRVLALRPEMTAPIARLVATSLRRRELPLRLGYSARVFRYEEPQAGR
CCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCHH
RREFHQAGVELIGAGGVAGDVEIIALAVESLAQAGLEDFRLGLGQVAVTKGVLQDLALPP
HHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCH
EAVAGIKSALASKDLVALERIYDEYHLEGERRRRLELLATIHGGREALEEARACFGRTAA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AASLAELSRVWEALGAAGLEKWLFIDLGILRDFDYYTGIVFEGYVPGLGAPVCGGGRYDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEEECCCCCCCCCCCCCCCCCC
LLAQFGYPCPATGFALGLERLLLARGETAPASLAGGYLVAGRDLAALLKRARELRSKGTA
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECHHHHHHHHHHHHHHCCCCE
VVLDGESRSRQEAAARAAARGLNLEWIGE
EEEECCCCHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA