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The map label for this gene is ligA

Identifier: 83590843

GI number: 83590843

Start: 2097721

End: 2099721

Strand: Reverse

Name: ligA

Synonym: Moth_2013

Alternate gene names: 83590843

Gene position: 2099721-2097721 (Counterclockwise)

Preceding gene: 83590844

Following gene: 83590842

Centisome position: 79.87

GC content: 62.62

Gene sequence:

>2001_bases
ATGGATTTAGCTGCTGCCCGGCAACGGGTGGAAGAATTGCGACGCCTGATCGAAGAACATAACTACCGCTACTACGTCCT
CGACCAACCCTCGATCAGCGACCGGGAATACGACGCCCTGATGCAGGAACTCATAGCCCTGGAGGAGGCTTACCCCGAAC
TCCGGACCCCGGACTCCCCCAGCCAGCGGGTAGGGGGTGCACCCCGGGAAGAGTTCAACCAGGTGCGCCATCCCCAGGTA
CTGCTGAGCCTCAACGACGCCTTTAATGAAGGCGACCTGCTGGAGTTTGACCGTCGCGTCCGGGATTTGGCGGGCCGCCC
GGTGGAATACGTCATCGAGGCTAAAATTGACGGCCTGGCTGTAGCCCTCACCTACAGGGACGGCCTTTTTACCCTGGGCG
CCACCCGGGGAGACGGCCAGGTGGGAGAGGAGGTCACTGCGAATCTCAAAACCATTCCCGCCCTGCCCCTGCGCCTGCGC
CGGCCACTGCCTTTTCTCGTGGTCCGAGGGGAGGTCTACATGCCCAAGGTCGCCTTTACCGCCCTGAATGCCGCCCGGGA
GGAAGCCGGGGAACCCCTCTTTGCCAACCCCCGTAACGCGGCCGCCGGTTCACTGCGCCAGCTGGACCCGAAAATAACGG
CCAGTCGCAGCTTGAGCCTCTTTGTTTATCAGGTAATCAGCCTTACCGGGGCGGAAGTTGCCACCCAGGCCGGGGCCCTG
GACTTCCTGGCGGAGCTGGGCTTTCCCGTCAACCCTTACCGGGTCGTGGCCCCGGACATTCAGGCCGTCCTGGAGGAGGT
AAAAGCCTGGACGCCGGAAAGGCGGGCGAGTTTACCCTATGAAATCGACGGCCTGGTCATCAAGGTCAACGACCTGGCCC
TTCATTCCGTCCTGGGGGCGACGGCCAAAGCCCCCCGTTGGGCTATGGCCTATAAATTCCCGGCTGAACAGGCCACCGCC
AGGGTGGAGGGGATTATCCTGCGGGTCGGGCGCACGGGGGTTCTGACCCCCACGGCCGTCCTCACCCCGGTCCGCCTGGC
CGGAACCACCGTTAGCCGGGCCACTTTACATAATGAAGATTATATCCGGGAGAAGGACATTCGCATCGGCGATACAGTCA
TTGTTCAGAAGGCCGGCGATATCATCCCGGAGGTGGTGGCTGTTATACCGGAGCGGCGTACCGGGTCGGAAGAGGTCTTT
ACCATGCCGGAGCGCTGCCCGGCCTGCGGGGCTGCGGTAGTGCGGCCGCCGGGGGAGGCGGCCCACCGCTGCACCGGCGG
TCTGGCCTGTCCGGCCCAGGTCCTGGAGGGGATTATCCACTTCGCTTCCCGGGGGGCCATGGACATCCAGGGCCTGGGTC
CGGCCATCGTGGCCCAGCTCCTGGAAGCCGGCCTCATCCACGACGCCGCCGATCTCTATTATTTAAAGGAAGAAGACCTG
TTAAAACTGGAGCGCTTCGGCCAGCAGTCGGCCAGCAATCTTCTGGCAGCCATTGCTGCCAGTAAAAAGCAACCCCTGGA
ACGCCTCATCTTTGCCCTGGGTATCCGTAACGTCGGTCAGCGGGCGGCCCGGGTCCTGGCCGATCATTTCGGTTCCCTGG
ATAAACTGGCCGCGGCCACGGTAGAGGAATTGACCGCCCTGCCCGATATCGGCCCCCGTATTGCCGAGAATATCCGGGAG
TTTTTTGGCGAACCCCGGAACCGGGCCGTACTGGAAAAGCTTAAAAAGGCCGGGGTGCGGCTGGAGGCTTTGAATGCAGC
AGCCCCGGCCGGACCCTTAACCGGCAAGGTCTTTGTCCTCACCGGCACCTTGCCGGGTATGACCCGCCAGGAAGCGGAGG
AACTGATCACCAGGGCGGGGGGTAAAGTCAGCAGCAGTGTGAGCCGGAAAACAGACTATGTAGTCGCCGGTGAAAAACCT
GGCTCCAAGTTAGACCGTGCCCGGGAACTGGGAGTGCCGGTAATCAACGCGGATGGGTTGCGACAACTCTTGCGCCAGTA
A

Upstream 100 bases:

>100_bases
GTTCAGTTTTCAGGAGTTGGCAGAGAGCCGTTGGGTAACTATTTGCGTGGTGGGACATAGCAGCGGCTGCACAGGAAATA
TTATCTCCGGGGGAATAGGT

Downstream 100 bases:

>100_bases
TCCTTTTGGGAGGCCCCGCGTTTGTTGCTTTATGCCGGCATACTCTTCGGCGGCGTCCATTTCACCGAGCGTTAGGTAAA
GGGGCGGGGAGATGCAGCAA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MDLAAARQRVEELRRLIEEHNYRYYVLDQPSISDREYDALMQELIALEEAYPELRTPDSPSQRVGGAPREEFNQVRHPQV
LLSLNDAFNEGDLLEFDRRVRDLAGRPVEYVIEAKIDGLAVALTYRDGLFTLGATRGDGQVGEEVTANLKTIPALPLRLR
RPLPFLVVRGEVYMPKVAFTALNAAREEAGEPLFANPRNAAAGSLRQLDPKITASRSLSLFVYQVISLTGAEVATQAGAL
DFLAELGFPVNPYRVVAPDIQAVLEEVKAWTPERRASLPYEIDGLVIKVNDLALHSVLGATAKAPRWAMAYKFPAEQATA
RVEGIILRVGRTGVLTPTAVLTPVRLAGTTVSRATLHNEDYIREKDIRIGDTVIVQKAGDIIPEVVAVIPERRTGSEEVF
TMPERCPACGAAVVRPPGEAAHRCTGGLACPAQVLEGIIHFASRGAMDIQGLGPAIVAQLLEAGLIHDAADLYYLKEEDL
LKLERFGQQSASNLLAAIAASKKQPLERLIFALGIRNVGQRAARVLADHFGSLDKLAAATVEELTALPDIGPRIAENIRE
FFGEPRNRAVLEKLKKAGVRLEALNAAAPAGPLTGKVFVLTGTLPGMTRQEAEELITRAGGKVSSSVSRKTDYVVAGEKP
GSKLDRARELGVPVINADGLRQLLRQ

Sequences:

>Translated_666_residues
MDLAAARQRVEELRRLIEEHNYRYYVLDQPSISDREYDALMQELIALEEAYPELRTPDSPSQRVGGAPREEFNQVRHPQV
LLSLNDAFNEGDLLEFDRRVRDLAGRPVEYVIEAKIDGLAVALTYRDGLFTLGATRGDGQVGEEVTANLKTIPALPLRLR
RPLPFLVVRGEVYMPKVAFTALNAAREEAGEPLFANPRNAAAGSLRQLDPKITASRSLSLFVYQVISLTGAEVATQAGAL
DFLAELGFPVNPYRVVAPDIQAVLEEVKAWTPERRASLPYEIDGLVIKVNDLALHSVLGATAKAPRWAMAYKFPAEQATA
RVEGIILRVGRTGVLTPTAVLTPVRLAGTTVSRATLHNEDYIREKDIRIGDTVIVQKAGDIIPEVVAVIPERRTGSEEVF
TMPERCPACGAAVVRPPGEAAHRCTGGLACPAQVLEGIIHFASRGAMDIQGLGPAIVAQLLEAGLIHDAADLYYLKEEDL
LKLERFGQQSASNLLAAIAASKKQPLERLIFALGIRNVGQRAARVLADHFGSLDKLAAATVEELTALPDIGPRIAENIRE
FFGEPRNRAVLEKLKKAGVRLEALNAAAPAGPLTGKVFVLTGTLPGMTRQEAEELITRAGGKVSSSVSRKTDYVVAGEKP
GSKLDRARELGVPVINADGLRQLLRQ
>Mature_666_residues
MDLAAARQRVEELRRLIEEHNYRYYVLDQPSISDREYDALMQELIALEEAYPELRTPDSPSQRVGGAPREEFNQVRHPQV
LLSLNDAFNEGDLLEFDRRVRDLAGRPVEYVIEAKIDGLAVALTYRDGLFTLGATRGDGQVGEEVTANLKTIPALPLRLR
RPLPFLVVRGEVYMPKVAFTALNAAREEAGEPLFANPRNAAAGSLRQLDPKITASRSLSLFVYQVISLTGAEVATQAGAL
DFLAELGFPVNPYRVVAPDIQAVLEEVKAWTPERRASLPYEIDGLVIKVNDLALHSVLGATAKAPRWAMAYKFPAEQATA
RVEGIILRVGRTGVLTPTAVLTPVRLAGTTVSRATLHNEDYIREKDIRIGDTVIVQKAGDIIPEVVAVIPERRTGSEEVF
TMPERCPACGAAVVRPPGEAAHRCTGGLACPAQVLEGIIHFASRGAMDIQGLGPAIVAQLLEAGLIHDAADLYYLKEEDL
LKLERFGQQSASNLLAAIAASKKQPLERLIFALGIRNVGQRAARVLADHFGSLDKLAAATVEELTALPDIGPRIAENIRE
FFGEPRNRAVLEKLKKAGVRLEALNAAAPAGPLTGKVFVLTGTLPGMTRQEAEELITRAGGKVSSSVSRKTDYVVAGEKP
GSKLDRARELGVPVINADGLRQLLRQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=74, Percent_Identity=50, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788750, Length=670, Percent_Identity=45.5223880597015, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI87082305, Length=587, Percent_Identity=23.679727427598, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI62472391, Length=108, Percent_Identity=39.8148148148148, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17737765, Length=108, Percent_Identity=40.7407407407407, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_MOOTA (Q2RGY0)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430852.1
- ProteinModelPortal:   Q2RGY0
- STRING:   Q2RGY0
- GeneID:   3831967
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2013
- NMPDR:   fig|264732.9.peg.2030
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- PhylomeDB:   Q2RGY0
- BioCyc:   MTHE264732:MOTH_2013-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 72387; Mature: 72387

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 171-171 BINDING 288-288 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAAARQRVEELRRLIEEHNYRYYVLDQPSISDREYDALMQELIALEEAYPELRTPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SQRVGGAPREEFNQVRHPQVLLSLNDAFNEGDLLEFDRRVRDLAGRPVEYVIEAKIDGLA
HHHCCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEE
VALTYRDGLFTLGATRGDGQVGEEVTANLKTIPALPLRLRRPLPFLVVRGEVYMPKVAFT
EEEEECCCEEEECCCCCCCCCCHHHHCCCHHCCCCCHHHCCCCCEEEEECCEECCHHHHH
ALNAAREEAGEPLFANPRNAAAGSLRQLDPKITASRSLSLFVYQVISLTGAEVATQAGAL
HHHHHHHHHCCCCCCCCCCCHHCCHHHCCCCHHHHCHHHHHHHHHHHHCCHHHHHHHHHH
DFLAELGFPVNPYRVVAPDIQAVLEEVKAWTPERRASLPYEIDGLVIKVNDLALHSVLGA
HHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCHHHCCCCEEECCEEEEECHHHHHHHHHC
TAKAPRWAMAYKFPAEQATARVEGIILRVGRTGVLTPTAVLTPVRLAGTTVSRATLHNED
CCCCCCEEEEEECCHHHHHHHHCEEEEEECCCCCCCCHHHHCCHHHCCCCHHHHHHCCCH
YIREKDIRIGDTVIVQKAGDIIPEVVAVIPERRTGSEEVFTMPERCPACGAAVVRPPGEA
HHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHEECHHHCCCCCCEEECCCCHH
AHRCTGGLACPAQVLEGIIHFASRGAMDIQGLGPAIVAQLLEAGLIHDAADLYYLKEEDL
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHEEECHHHH
LKLERFGQQSASNLLAAIAASKKQPLERLIFALGIRNVGQRAARVLADHFGSLDKLAAAT
HHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VEELTALPDIGPRIAENIREFFGEPRNRAVLEKLKKAGVRLEALNAAAPAGPLTGKVFVL
HHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEE
TGTLPGMTRQEAEELITRAGGKVSSSVSRKTDYVVAGEKPGSKLDRARELGVPVINADGL
ECCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCEECHHHH
RQLLRQ
HHHHHC
>Mature Secondary Structure
MDLAAARQRVEELRRLIEEHNYRYYVLDQPSISDREYDALMQELIALEEAYPELRTPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SQRVGGAPREEFNQVRHPQVLLSLNDAFNEGDLLEFDRRVRDLAGRPVEYVIEAKIDGLA
HHHCCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEE
VALTYRDGLFTLGATRGDGQVGEEVTANLKTIPALPLRLRRPLPFLVVRGEVYMPKVAFT
EEEEECCCEEEECCCCCCCCCCHHHHCCCHHCCCCCHHHCCCCCEEEEECCEECCHHHHH
ALNAAREEAGEPLFANPRNAAAGSLRQLDPKITASRSLSLFVYQVISLTGAEVATQAGAL
HHHHHHHHHCCCCCCCCCCCHHCCHHHCCCCHHHHCHHHHHHHHHHHHCCHHHHHHHHHH
DFLAELGFPVNPYRVVAPDIQAVLEEVKAWTPERRASLPYEIDGLVIKVNDLALHSVLGA
HHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCHHHCCCCEEECCEEEEECHHHHHHHHHC
TAKAPRWAMAYKFPAEQATARVEGIILRVGRTGVLTPTAVLTPVRLAGTTVSRATLHNED
CCCCCCEEEEEECCHHHHHHHHCEEEEEECCCCCCCCHHHHCCHHHCCCCHHHHHHCCCH
YIREKDIRIGDTVIVQKAGDIIPEVVAVIPERRTGSEEVFTMPERCPACGAAVVRPPGEA
HHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHEECHHHCCCCCCEEECCCCHH
AHRCTGGLACPAQVLEGIIHFASRGAMDIQGLGPAIVAQLLEAGLIHDAADLYYLKEEDL
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHEEECHHHH
LKLERFGQQSASNLLAAIAASKKQPLERLIFALGIRNVGQRAARVLADHFGSLDKLAAAT
HHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VEELTALPDIGPRIAENIREFFGEPRNRAVLEKLKKAGVRLEALNAAAPAGPLTGKVFVL
HHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEE
TGTLPGMTRQEAEELITRAGGKVSSSVSRKTDYVVAGEKPGSKLDRARELGVPVINADGL
ECCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCEECHHHH
RQLLRQ
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA