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The map label for this gene is pcrA [H]

Identifier: 83590844

GI number: 83590844

Start: 2099878

End: 2102067

Strand: Reverse

Name: pcrA [H]

Synonym: Moth_2014

Alternate gene names: 83590844

Gene position: 2102067-2099878 (Counterclockwise)

Preceding gene: 83590846

Following gene: 83590843

Centisome position: 79.96

GC content: 61.1

Gene sequence:

>2190_bases
GTGAAGGTATTGGCAGATTTTATGGCTTTACTCAATGGTCCCCAGCAGGAGGCCGTCAAGCACCGGGGCACACCCCTCCT
GGTCCTGGCCGGAGCCGGCAGCGGCAAGACCCGGGTCCTTACTTACCGGGTGGCCGCCCTGATTCAGGAAGGTGTTCGCC
CGGAAAACATCCTCGCGGTTACCTTTACCAATAAAGCGGCCCAGGAAATGAAAGAACGCCTGGAGGGCCTGGTAGGGGAG
GCGGCCCGGGGCCTCTGGGTCAGCACCTTCCATTCAGCCTGTGTCCGCATTCTGCGGCGGGAGGCCCACCTCCTTGGTTA
TCGGCCGAATTTTGTCATCTACGATACCGACGATCAGCAGGCGGCCCTTAAAGAGGTCTTAAAAGAGTTAGACCTGGATG
ATAAAAAATATCCGCCTCGTTCCCTGGCTCAGGTCATTAGCATGGCCAAGAACGACCTTAAGACACCGGAGCGTTTCCTG
GACGGCGCGGCCACCTTCCGGGAGCAACAGCAGGGGCAGATTTACCGCCGTTATCAGGAGAAGCTGCGGGAATTAAACGC
CATGGACTTTGACGATCTTATCATGCAAACGGTCTTTCTCTGGCAGCAGAATCCCCTGGTATTACGTTACTACCAGCAGC
GCTGGCAGCATATCCTGGTGGACGAATACCAGGATACCAACCACGCCCAGTACATCCTGGTGCGTCTCCTTGCCGGTAAA
GGGGACAACCTCTGTGTCGTCGGCGACCCGGATCAGGGTATCTACGGCTGGCGGGGAGCTGATATCGGTAACATACTGGC
CTTTGAGGAGGACTTCCCCCGGGCCCGGGTGATTCTCCTGGAAGAAAACTACCGGTCCACCCGGCCCATCCTCCAGGCCG
CCAATGCCGTTATCCAGCATAATGAGGGGCGCCGGGAGAAACGCCTCTGGACACGGCGGCGGGAAGGAGAGCTGTTGCAC
CTGTACCGGGCGACTGACGAGCGGGATGAGGGGCGGTTTATCGCCGGCGAGGTTTACCGCCGGCACCAGCAGGAGGGGCG
GCCCTTCAGCGACTTTGCCGTCCTCTACCGCACCCATGCCCAGTCGCGGGCCCTGGAAGAGGCCTTTATCCAGGCCGGTG
TCCCCTACGAGATTGTCGGCGGCCTGAAGTTCTACCAGCGCAAGGAGATTAAGGATATCCTGGCCTATCTGCGGGTAATT
GCCAACCCGGACGATAGCCTCAGCCTGTTGCGGATTATCAACGTCCCCCGGCGGGGTATCGGCGAGGCCACCCTGGCCAG
GCTGGAAGCCGCCGCTACCAGTGAGGGGGAGAGCCTTTACCGGGTCCTGGAGCGGGTGGACACCATCCCCGGCATCCCGG
CCCGGGGCCGGCAGGCACTGCGGGAACTGGTGGAAATGCTGGATAACCTTCGCCAGCAGCAGGAAAAAATAACGGTGACG
GACCTGGTGGCTACCATCTTGCAAGAGACGGGTTATCAAGCAGAACTGGAGGCGGAGCGGACTCCTGAGGCCCAGGCCCG
GCTGGAGAACTTGAAGGAATTCCAGACGGTGACCCGGAGCTACGACCAGGGGGCTCCGGAATCATCCCTGGGAGATTTCC
TGACCCAGGTAGCCCTGGTGGCGGAGAGTGACACCTATAGCGGCAATGCCGCGGTGGCCTTAATGACCATGCATACGGCC
AAGGGACTGGAATTCCCGGTGGTCTTCCTGGCCGGCCTAGAAGAGGGGGTTTTCCCCCATTTTCGCTCCCTGGACGACCC
GGCGGAGATGGAAGAAGAACGGCGACTCTGCTATGTGGGCATGACCAGGGCCAGGGAGGTCCTGTATCTCACCCATGCCT
GGACGCGTAACCTTTACGGTAGCACCATGAGCAATCCTCCCTCCCGGTTTCTGGATGAGATACCGGCCGACTTGATCCAG
GGCGAAGGAACCGGCCTGCGGTCCGGGGTCCTTAGCCGGACCAGTGAGGAACGGGATGACCGGGGCCGATCAACCCGGCG
CCAGCCGGCACCGGCAAGGAGCACCCGGGCCTCCTGGCAGGTGGGGGACAAGGTCCAGCATGACGCCTGGGGCCTGGGCG
TCATAGTAAAGATCAGCGGCGAAGGCGATGATGCCATCATCAGCGTCGCCTTTCCCGGGCGGGGCATCAAGCAACTGGCC
CTCCGCTACGCCCCGATTAGGAAGGCTTAA

Upstream 100 bases:

>100_bases
TGCTGCATTATATGCATTACCTTCCTGGAAGGGGAAAAAGAAGGCCGTTTCCTTGCAGGTTTTTTGGCTGCCGGGGCGAA
TATAACCAGGGCTTAGGAGT

Downstream 100 bases:

>100_bases
GTACGCGTAACCCTATGGGAGCTCTTTATGGAGACGCGCCGCTGCTATCCTGGGAAGTTCAGTTTTCAGGAGTTGGCAGA
GAGCCGTTGGGTAACTATTT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 729; Mature: 729

Protein sequence:

>729_residues
MKVLADFMALLNGPQQEAVKHRGTPLLVLAGAGSGKTRVLTYRVAALIQEGVRPENILAVTFTNKAAQEMKERLEGLVGE
AARGLWVSTFHSACVRILRREAHLLGYRPNFVIYDTDDQQAALKEVLKELDLDDKKYPPRSLAQVISMAKNDLKTPERFL
DGAATFREQQQGQIYRRYQEKLRELNAMDFDDLIMQTVFLWQQNPLVLRYYQQRWQHILVDEYQDTNHAQYILVRLLAGK
GDNLCVVGDPDQGIYGWRGADIGNILAFEEDFPRARVILLEENYRSTRPILQAANAVIQHNEGRREKRLWTRRREGELLH
LYRATDERDEGRFIAGEVYRRHQQEGRPFSDFAVLYRTHAQSRALEEAFIQAGVPYEIVGGLKFYQRKEIKDILAYLRVI
ANPDDSLSLLRIINVPRRGIGEATLARLEAAATSEGESLYRVLERVDTIPGIPARGRQALRELVEMLDNLRQQQEKITVT
DLVATILQETGYQAELEAERTPEAQARLENLKEFQTVTRSYDQGAPESSLGDFLTQVALVAESDTYSGNAAVALMTMHTA
KGLEFPVVFLAGLEEGVFPHFRSLDDPAEMEEERRLCYVGMTRAREVLYLTHAWTRNLYGSTMSNPPSRFLDEIPADLIQ
GEGTGLRSGVLSRTSEERDDRGRSTRRQPAPARSTRASWQVGDKVQHDAWGLGVIVKISGEGDDAIISVAFPGRGIKQLA
LRYAPIRKA

Sequences:

>Translated_729_residues
MKVLADFMALLNGPQQEAVKHRGTPLLVLAGAGSGKTRVLTYRVAALIQEGVRPENILAVTFTNKAAQEMKERLEGLVGE
AARGLWVSTFHSACVRILRREAHLLGYRPNFVIYDTDDQQAALKEVLKELDLDDKKYPPRSLAQVISMAKNDLKTPERFL
DGAATFREQQQGQIYRRYQEKLRELNAMDFDDLIMQTVFLWQQNPLVLRYYQQRWQHILVDEYQDTNHAQYILVRLLAGK
GDNLCVVGDPDQGIYGWRGADIGNILAFEEDFPRARVILLEENYRSTRPILQAANAVIQHNEGRREKRLWTRRREGELLH
LYRATDERDEGRFIAGEVYRRHQQEGRPFSDFAVLYRTHAQSRALEEAFIQAGVPYEIVGGLKFYQRKEIKDILAYLRVI
ANPDDSLSLLRIINVPRRGIGEATLARLEAAATSEGESLYRVLERVDTIPGIPARGRQALRELVEMLDNLRQQQEKITVT
DLVATILQETGYQAELEAERTPEAQARLENLKEFQTVTRSYDQGAPESSLGDFLTQVALVAESDTYSGNAAVALMTMHTA
KGLEFPVVFLAGLEEGVFPHFRSLDDPAEMEEERRLCYVGMTRAREVLYLTHAWTRNLYGSTMSNPPSRFLDEIPADLIQ
GEGTGLRSGVLSRTSEERDDRGRSTRRQPAPARSTRASWQVGDKVQHDAWGLGVIVKISGEGDDAIISVAFPGRGIKQLA
LRYAPIRKA
>Mature_729_residues
MKVLADFMALLNGPQQEAVKHRGTPLLVLAGAGSGKTRVLTYRVAALIQEGVRPENILAVTFTNKAAQEMKERLEGLVGE
AARGLWVSTFHSACVRILRREAHLLGYRPNFVIYDTDDQQAALKEVLKELDLDDKKYPPRSLAQVISMAKNDLKTPERFL
DGAATFREQQQGQIYRRYQEKLRELNAMDFDDLIMQTVFLWQQNPLVLRYYQQRWQHILVDEYQDTNHAQYILVRLLAGK
GDNLCVVGDPDQGIYGWRGADIGNILAFEEDFPRARVILLEENYRSTRPILQAANAVIQHNEGRREKRLWTRRREGELLH
LYRATDERDEGRFIAGEVYRRHQQEGRPFSDFAVLYRTHAQSRALEEAFIQAGVPYEIVGGLKFYQRKEIKDILAYLRVI
ANPDDSLSLLRIINVPRRGIGEATLARLEAAATSEGESLYRVLERVDTIPGIPARGRQALRELVEMLDNLRQQQEKITVT
DLVATILQETGYQAELEAERTPEAQARLENLKEFQTVTRSYDQGAPESSLGDFLTQVALVAESDTYSGNAAVALMTMHTA
KGLEFPVVFLAGLEEGVFPHFRSLDDPAEMEEERRLCYVGMTRAREVLYLTHAWTRNLYGSTMSNPPSRFLDEIPADLIQ
GEGTGLRSGVLSRTSEERDDRGRSTRRQPAPARSTRASWQVGDKVQHDAWGLGVIVKISGEGDDAIISVAFPGRGIKQLA
LRYAPIRKA

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=722, Percent_Identity=41.6897506925208, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI48994965, Length=639, Percent_Identity=40.8450704225352, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1787196, Length=345, Percent_Identity=29.2753623188406, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=714, Percent_Identity=27.8711484593838, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324477, Length=656, Percent_Identity=24.6951219512195, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82563; Mature: 82563

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLADFMALLNGPQQEAVKHRGTPLLVLAGAGSGKTRVLTYRVAALIQEGVRPENILAV
CCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCEEEE
TFTNKAAQEMKERLEGLVGEAARGLWVSTFHSACVRILRREAHLLGYRPNFVIYDTDDQQ
EECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHH
AALKEVLKELDLDDKKYPPRSLAQVISMAKNDLKTPERFLDGAATFREQQQGQIYRRYQE
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KLRELNAMDFDDLIMQTVFLWQQNPLVLRYYQQRWQHILVDEYQDTNHAQYILVRLLAGK
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
GDNLCVVGDPDQGIYGWRGADIGNILAFEEDFPRARVILLEENYRSTRPILQAANAVIQH
CCCEEEECCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHC
NEGRREKRLWTRRREGELLHLYRATDERDEGRFIAGEVYRRHQQEGRPFSDFAVLYRTHA
CCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHH
QSRALEEAFIQAGVPYEIVGGLKFYQRKEIKDILAYLRVIANPDDSLSLLRIINVPRRGI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
GEATLARLEAAATSEGESLYRVLERVDTIPGIPARGRQALRELVEMLDNLRQQQEKITVT
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVATILQETGYQAELEAERTPEAQARLENLKEFQTVTRSYDQGAPESSLGDFLTQVALV
HHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AESDTYSGNAAVALMTMHTAKGLEFPVVFLAGLEEGVFPHFRSLDDPAEMEEERRLCYVG
CCCCCCCCCCEEEEEEEHHHCCCCCHHHHHHCCCCCCCCHHHCCCCHHHHHHHCCEEEEE
MTRAREVLYLTHAWTRNLYGSTMSNPPSRFLDEIPADLIQGEGTGLRSGVLSRTSEERDD
HHHHHHEEEEHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCCCCHHHHHHHCCCHHHHH
RGRSTRRQPAPARSTRASWQVGDKVQHDAWGLGVIVKISGEGDDAIISVAFPGRGIKQLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHH
LRYAPIRKA
HHHCCCCCC
>Mature Secondary Structure
MKVLADFMALLNGPQQEAVKHRGTPLLVLAGAGSGKTRVLTYRVAALIQEGVRPENILAV
CCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCEEEE
TFTNKAAQEMKERLEGLVGEAARGLWVSTFHSACVRILRREAHLLGYRPNFVIYDTDDQQ
EECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHH
AALKEVLKELDLDDKKYPPRSLAQVISMAKNDLKTPERFLDGAATFREQQQGQIYRRYQE
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KLRELNAMDFDDLIMQTVFLWQQNPLVLRYYQQRWQHILVDEYQDTNHAQYILVRLLAGK
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
GDNLCVVGDPDQGIYGWRGADIGNILAFEEDFPRARVILLEENYRSTRPILQAANAVIQH
CCCEEEECCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHC
NEGRREKRLWTRRREGELLHLYRATDERDEGRFIAGEVYRRHQQEGRPFSDFAVLYRTHA
CCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHH
QSRALEEAFIQAGVPYEIVGGLKFYQRKEIKDILAYLRVIANPDDSLSLLRIINVPRRGI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
GEATLARLEAAATSEGESLYRVLERVDTIPGIPARGRQALRELVEMLDNLRQQQEKITVT
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVATILQETGYQAELEAERTPEAQARLENLKEFQTVTRSYDQGAPESSLGDFLTQVALV
HHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AESDTYSGNAAVALMTMHTAKGLEFPVVFLAGLEEGVFPHFRSLDDPAEMEEERRLCYVG
CCCCCCCCCCEEEEEEEHHHCCCCCHHHHHHCCCCCCCCHHHCCCCHHHHHHHCCEEEEE
MTRAREVLYLTHAWTRNLYGSTMSNPPSRFLDEIPADLIQGEGTGLRSGVLSRTSEERDD
HHHHHHEEEEHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCCCCCCHHHHHHHCCCHHHHH
RGRSTRRQPAPARSTRASWQVGDKVQHDAWGLGVIVKISGEGDDAIISVAFPGRGIKQLA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHH
LRYAPIRKA
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA