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The map label for this gene is gatB

Identifier: 83590838

GI number: 83590838

Start: 2093275

End: 2094705

Strand: Reverse

Name: gatB

Synonym: Moth_2008

Alternate gene names: 83590838

Gene position: 2094705-2093275 (Counterclockwise)

Preceding gene: 83590839

Following gene: 83590837

Centisome position: 79.68

GC content: 61.29

Gene sequence:

>1431_bases
ATGGAATACGAAGCTGTAATCGGCCTGGAAGTCCATGCGGAACTGAAGACGGCTTCCAAGGCTTTCTGTAGCTGTAGTAC
GGCCTTTGGCGGCGAGCCCAATACCCACGTCTGCCCTGTTTGCCTGGGGCTGCCCGGGGTATTGCCGGTTATTAACCGCC
AGGTGGTTGAGTTCGGTCTGAAAACGGCCCTGGCCCTCAACTGCCGGGTGGCGCCCTTCTGTAAATTCGACCGCAAGAAC
TACTACTACCCCGATCTCCCCAAGAACTACCAGATTTCCCAGTACGACCTGCCCCTGGCTACCGGCGGCTACCTGAAGAT
CAACGTCGACGGCCAGGAGCGGGTAATCGGTATCACCCGGGTGCATATGGAAGAAGACGCCGGCAAACTCGTCCACGTGG
ACGGACCCGGCGGGGGTTACTCCCTGGTGGACTACAACCGCACCGGCGTACCCCTGCTGGAAATCGTCTCCGAGCCGGAC
CTGCGCTCTCCGGCGGAGGCCCGGGCTTATATGGAAAAGCTCAGGGCCATCCTCCAGTACCTGGACGTTTCCGACTGCAA
GATGGAAGAGGGATCCTTGCGCTGCGACGCCAATGTCTCGGTGCGGCCCCGGGGTTCTCAAACCTTCGGCACCAAGACGG
AAGTAAAGAATATGAACTCCTTCCGCGCCCTGCAGCGGGCCCTGGAGTACGAGATTGAGCGCCAGATTGCCATTCTGGAG
GGCGGCGGCCGGGTCGAGCAGGCGACCATGGCGTGGGATGAAAGCCGCGGGGTAACAACCGTTATGCGGACCAAGGAGCA
GGCCCACGACTACCGCTACTTCCCGGAACCGGACCTGGTACCCCTGGAAATCGACGCCGCCTGGATCGAACGGGTACGCC
GGGAGCTTCCGGAGCTGCCCGATGCCCGTTGCCGGCGTTTAATGGATACCTTCGGCCTGCCGGCCTACGATGCCGGGGTG
ATAACCAGCTCCCGGGACCTGGCCGACTACTTCGACCGGGTAGTGGCCCGGTATCCCGATGCCAAGGTTGTCAGCAACTG
GATCATGGGCGACTTCTTACGGCTGTTAAACGCCCGGAACCTTGAGCCCGGCCAGGCGCCGGTACCACCCGAGGAACTGG
CAGACCTCCTTGAGTTGCAGAAGGAAGGCACCATCAGCGGTAAGATCGCCAAGCAGGTCCTGGAGGAGATGTTTGCCAGC
GGCAAAGGCGCCCGCCAGATTGTCCAGGAAAGGGGCCTGGTCCAGATCAGCGACACCGCCGCCCTGGGGAAGATTGTCGA
CGAGGTGCTGGCAGCCAATCCCAACGTGGTGGAGGACTACCGCAACGGCAAGGAAAAGGCTCTGGGCTTTCTGGTGGGCC
AGGTAATGAAGGCGACAGGGGGGAAAGCCAACCCCGGCCTGGTGAACAAGCTCCTTAAGGAAAGGTTATAG

Upstream 100 bases:

>100_bases
TTGGTCGGCCCTTTGCCGAGGCCACCCTGTTGCAGGCAGCTTATGCCTTTGAACAGAGTACGGAATACCACCGGCGGCGG
CCGGAACTGGGGGTGGCGTA

Downstream 100 bases:

>100_bases
GGAGGCCTGCCCTTGTTTGCCGGCGTACTTTTTGATTTTGACGGCACCCTGGTGGATACTTCCGAGCTGGTTATCAAGTC
CTTCCAGCATACCCTGGCGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MEYEAVIGLEVHAELKTASKAFCSCSTAFGGEPNTHVCPVCLGLPGVLPVINRQVVEFGLKTALALNCRVAPFCKFDRKN
YYYPDLPKNYQISQYDLPLATGGYLKINVDGQERVIGITRVHMEEDAGKLVHVDGPGGGYSLVDYNRTGVPLLEIVSEPD
LRSPAEARAYMEKLRAILQYLDVSDCKMEEGSLRCDANVSVRPRGSQTFGTKTEVKNMNSFRALQRALEYEIERQIAILE
GGGRVEQATMAWDESRGVTTVMRTKEQAHDYRYFPEPDLVPLEIDAAWIERVRRELPELPDARCRRLMDTFGLPAYDAGV
ITSSRDLADYFDRVVARYPDAKVVSNWIMGDFLRLLNARNLEPGQAPVPPEELADLLELQKEGTISGKIAKQVLEEMFAS
GKGARQIVQERGLVQISDTAALGKIVDEVLAANPNVVEDYRNGKEKALGFLVGQVMKATGGKANPGLVNKLLKERL

Sequences:

>Translated_476_residues
MEYEAVIGLEVHAELKTASKAFCSCSTAFGGEPNTHVCPVCLGLPGVLPVINRQVVEFGLKTALALNCRVAPFCKFDRKN
YYYPDLPKNYQISQYDLPLATGGYLKINVDGQERVIGITRVHMEEDAGKLVHVDGPGGGYSLVDYNRTGVPLLEIVSEPD
LRSPAEARAYMEKLRAILQYLDVSDCKMEEGSLRCDANVSVRPRGSQTFGTKTEVKNMNSFRALQRALEYEIERQIAILE
GGGRVEQATMAWDESRGVTTVMRTKEQAHDYRYFPEPDLVPLEIDAAWIERVRRELPELPDARCRRLMDTFGLPAYDAGV
ITSSRDLADYFDRVVARYPDAKVVSNWIMGDFLRLLNARNLEPGQAPVPPEELADLLELQKEGTISGKIAKQVLEEMFAS
GKGARQIVQERGLVQISDTAALGKIVDEVLAANPNVVEDYRNGKEKALGFLVGQVMKATGGKANPGLVNKLLKERL
>Mature_476_residues
MEYEAVIGLEVHAELKTASKAFCSCSTAFGGEPNTHVCPVCLGLPGVLPVINRQVVEFGLKTALALNCRVAPFCKFDRKN
YYYPDLPKNYQISQYDLPLATGGYLKINVDGQERVIGITRVHMEEDAGKLVHVDGPGGGYSLVDYNRTGVPLLEIVSEPD
LRSPAEARAYMEKLRAILQYLDVSDCKMEEGSLRCDANVSVRPRGSQTFGTKTEVKNMNSFRALQRALEYEIERQIAILE
GGGRVEQATMAWDESRGVTTVMRTKEQAHDYRYFPEPDLVPLEIDAAWIERVRRELPELPDARCRRLMDTFGLPAYDAGV
ITSSRDLADYFDRVVARYPDAKVVSNWIMGDFLRLLNARNLEPGQAPVPPEELADLLELQKEGTISGKIAKQVLEEMFAS
GKGARQIVQERGLVQISDTAALGKIVDEVLAANPNVVEDYRNGKEKALGFLVGQVMKATGGKANPGLVNKLLKERL

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=39.0342052313883, Blast_Score=321, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI115534094, Length=476, Percent_Identity=31.7226890756302, Blast_Score=221, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=29.0384615384615, Blast_Score=229, Evalue=9e-61,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=35.6997971602434, Blast_Score=298, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_MOOTA (Q2RGY5)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430847.1
- ProteinModelPortal:   Q2RGY5
- SMR:   Q2RGY5
- STRING:   Q2RGY5
- GeneID:   3831962
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2008
- NMPDR:   fig|264732.9.peg.2026
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2RGY5
- ProtClustDB:   PRK05477
- BioCyc:   MTHE264732:MOTH_2008-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52786; Mature: 52786

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYEAVIGLEVHAELKTASKAFCSCSTAFGGEPNTHVCPVCLGLPGVLPVINRQVVEFGL
CCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHH
KTALALNCRVAPFCKFDRKNYYYPDLPKNYQISQYDLPLATGGYLKINVDGQERVIGITR
HHHHEECCCCCCCEEECCCCCCCCCCCCCCCCCEECCCEECCCEEEEEECCCCEEEEEEE
VHMEEDAGKLVHVDGPGGGYSLVDYNRTGVPLLEIVSEPDLRSPAEARAYMEKLRAILQY
EEEECCCCCEEEEECCCCCCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LDVSDCKMEEGSLRCDANVSVRPRGSQTFGTKTEVKNMNSFRALQRALEYEIERQIAILE
HCCCCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHEEEEEE
GGGRVEQATMAWDESRGVTTVMRTKEQAHDYRYFPEPDLVPLEIDAAWIERVRRELPELP
CCCCEEHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHCCCCC
DARCRRLMDTFGLPAYDAGVITSSRDLADYFDRVVARYPDAKVVSNWIMGDFLRLLNARN
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
LEPGQAPVPPEELADLLELQKEGTISGKIAKQVLEEMFASGKGARQIVQERGLVQISDTA
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECHH
ALGKIVDEVLAANPNVVEDYRNGKEKALGFLVGQVMKATGGKANPGLVNKLLKERL
HHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MEYEAVIGLEVHAELKTASKAFCSCSTAFGGEPNTHVCPVCLGLPGVLPVINRQVVEFGL
CCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHH
KTALALNCRVAPFCKFDRKNYYYPDLPKNYQISQYDLPLATGGYLKINVDGQERVIGITR
HHHHEECCCCCCCEEECCCCCCCCCCCCCCCCCEECCCEECCCEEEEEECCCCEEEEEEE
VHMEEDAGKLVHVDGPGGGYSLVDYNRTGVPLLEIVSEPDLRSPAEARAYMEKLRAILQY
EEEECCCCCEEEEECCCCCCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LDVSDCKMEEGSLRCDANVSVRPRGSQTFGTKTEVKNMNSFRALQRALEYEIERQIAILE
HCCCCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHEEEEEE
GGGRVEQATMAWDESRGVTTVMRTKEQAHDYRYFPEPDLVPLEIDAAWIERVRRELPELP
CCCCEEHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHCCCCC
DARCRRLMDTFGLPAYDAGVITSSRDLADYFDRVVARYPDAKVVSNWIMGDFLRLLNARN
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
LEPGQAPVPPEELADLLELQKEGTISGKIAKQVLEEMFASGKGARQIVQERGLVQISDTA
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECHH
ALGKIVDEVLAANPNVVEDYRNGKEKALGFLVGQVMKATGGKANPGLVNKLLKERL
HHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA