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The map label for this gene is gatA

Identifier: 83590839

GI number: 83590839

Start: 2094705

End: 2096168

Strand: Reverse

Name: gatA

Synonym: Moth_2009

Alternate gene names: 83590839

Gene position: 2096168-2094705 (Counterclockwise)

Preceding gene: 83590840

Following gene: 83590838

Centisome position: 79.74

GC content: 62.98

Gene sequence:

>1464_bases
ATGGAATTATATTACTTAACAGCCCATGAATTGAGCGACCTGTTGAACCGTAAGGAAATCAGCTCCGAAGAAGCCACGGC
GGCCATCATTGACCGGATTGAAGCCGTCGACGGCCGGGTCCAGGCTTATCTAACCCGGACGGCGGAACAGGCCCTGGAAG
AGGCCCGGGCTGTGGATGCCGCCCGGGCCCGGGGTGAAACCCTGGGACCCCTGGCCGGGGTGCCCATGGCCCTGAAGGAC
AATCTCTGTACGGAGGGGGTGCGGACCACCTGTTCGTCCCGGATGCTGGCAGACTGGGTGCCTCCCTATGACGCCACCGT
GGTCCGGCGTCTTAAAGAGGCCGGGGCGGTGATGCTGGGCAAGCTCAATATGGACGAGTTCGCCATGGGTTCTTCCACGG
AGAATTCCAGTTTCTTCCCCACCCGGAATCCCTGGGACCTGGAGCGGGTGCCGGGAGGTTCCAGCGGCGGCGCCGTGGCG
GCGGTGGCTGCCGGCGAAGCCTATTTCGCCTTAGGGTCGGATACGGGCGGTTCCATCCGCCAGCCGGCTTCCTTCTGTGG
CGTCGTGGGGATGAAGCCCACCTACGGCCGGGTATCCCGTTACGGCCTGGTGGCCTTCGCCTCCTCCCTGGACCAGATCG
GCCCCATTACCCGCGACGTCACCGATTGCGCCCTGGTCCTGGAGGCCATTGCCGGGCATGACCCCCTGGATTCTACGTCC
GCCGATCTGCCGGTGCCCGATTACCGGTCGGCCCTGAAGCCGGAAGTGAAAGGCCTGAAGATAGGCGTTCCGCGGGAGTA
TTTCGGCGCTGGCATGGAACCGGAGGTGGCGGCCATTGTCCGGCGGGCTATTGCCAAACTGGAGGAACTGGGAGCCGTTT
GTGAGGAAACCTCCCTGCCCCATACCGAGTACGCCCTGCCGGCCTATTACCTGGTGGCCCCGGCCGAGGCCTCGTCCAAC
CTGGCCCGTTATGACGGCGTCAGTTACGGCCTGCGGGTGCCGGGCAAGGATATTATCGAGATGTACATGAATACCCGCAG
CCAGGGTTTCGGCCCCGAGGTCAAACGGCGGATTATGCTCGGCACCTATGCCTTGAGCTCGGGCTACTATGATGCCTATT
ACCTGAAGGCCCTTAAGGTACGAACCCTTATCCGCCGGGACTTTGAAACGGCCTTCGAGAAATACGATCTCCTGGCCACG
CCTACCTCCCCGACGGTGGCCTTCCGCCTGGGTGAAAAGGCGGGAGACCCCCTGGCCATGTACCTGTCCGACCTGTGTAC
CATCCCTATTAACATGGCCGGGCTACCGGCCCTGTCTTTGCCCTGCGGTTTCAGCCAGGGACTACCCGTCGGCCTGCAGC
TGATTGGTCGGCCCTTTGCCGAGGCCACCCTGTTGCAGGCAGCTTATGCCTTTGAACAGAGTACGGAATACCACCGGCGG
CGGCCGGAACTGGGGGTGGCGTAA

Upstream 100 bases:

>100_bases
GGCCCGGACTGCCCCGGGAGAAAGCCCTGCAGGGGGCTCCCGAAGTTAGCGAAGGCCAGTTTAAAGTACCGCGGGTAGTA
TAGGCCGGGAGGTAGAAAAG

Downstream 100 bases:

>100_bases
TGGAATACGAAGCTGTAATCGGCCTGGAAGTCCATGCGGAACTGAAGACGGCTTCCAAGGCTTTCTGTAGCTGTAGTACG
GCCTTTGGCGGCGAGCCCAA

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MELYYLTAHELSDLLNRKEISSEEATAAIIDRIEAVDGRVQAYLTRTAEQALEEARAVDAARARGETLGPLAGVPMALKD
NLCTEGVRTTCSSRMLADWVPPYDATVVRRLKEAGAVMLGKLNMDEFAMGSSTENSSFFPTRNPWDLERVPGGSSGGAVA
AVAAGEAYFALGSDTGGSIRQPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPITRDVTDCALVLEAIAGHDPLDSTS
ADLPVPDYRSALKPEVKGLKIGVPREYFGAGMEPEVAAIVRRAIAKLEELGAVCEETSLPHTEYALPAYYLVAPAEASSN
LARYDGVSYGLRVPGKDIIEMYMNTRSQGFGPEVKRRIMLGTYALSSGYYDAYYLKALKVRTLIRRDFETAFEKYDLLAT
PTSPTVAFRLGEKAGDPLAMYLSDLCTIPINMAGLPALSLPCGFSQGLPVGLQLIGRPFAEATLLQAAYAFEQSTEYHRR
RPELGVA

Sequences:

>Translated_487_residues
MELYYLTAHELSDLLNRKEISSEEATAAIIDRIEAVDGRVQAYLTRTAEQALEEARAVDAARARGETLGPLAGVPMALKD
NLCTEGVRTTCSSRMLADWVPPYDATVVRRLKEAGAVMLGKLNMDEFAMGSSTENSSFFPTRNPWDLERVPGGSSGGAVA
AVAAGEAYFALGSDTGGSIRQPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPITRDVTDCALVLEAIAGHDPLDSTS
ADLPVPDYRSALKPEVKGLKIGVPREYFGAGMEPEVAAIVRRAIAKLEELGAVCEETSLPHTEYALPAYYLVAPAEASSN
LARYDGVSYGLRVPGKDIIEMYMNTRSQGFGPEVKRRIMLGTYALSSGYYDAYYLKALKVRTLIRRDFETAFEKYDLLAT
PTSPTVAFRLGEKAGDPLAMYLSDLCTIPINMAGLPALSLPCGFSQGLPVGLQLIGRPFAEATLLQAAYAFEQSTEYHRR
RPELGVA
>Mature_487_residues
MELYYLTAHELSDLLNRKEISSEEATAAIIDRIEAVDGRVQAYLTRTAEQALEEARAVDAARARGETLGPLAGVPMALKD
NLCTEGVRTTCSSRMLADWVPPYDATVVRRLKEAGAVMLGKLNMDEFAMGSSTENSSFFPTRNPWDLERVPGGSSGGAVA
AVAAGEAYFALGSDTGGSIRQPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPITRDVTDCALVLEAIAGHDPLDSTS
ADLPVPDYRSALKPEVKGLKIGVPREYFGAGMEPEVAAIVRRAIAKLEELGAVCEETSLPHTEYALPAYYLVAPAEASSN
LARYDGVSYGLRVPGKDIIEMYMNTRSQGFGPEVKRRIMLGTYALSSGYYDAYYLKALKVRTLIRRDFETAFEKYDLLAT
PTSPTVAFRLGEKAGDPLAMYLSDLCTIPINMAGLPALSLPCGFSQGLPVGLQLIGRPFAEATLLQAAYAFEQSTEYHRR
RPELGVA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=41.4141414141414, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI195972892, Length=490, Percent_Identity=26.1224489795918, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI166795287, Length=201, Percent_Identity=37.3134328358209, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=37.7272727272727, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=497, Percent_Identity=26.5593561368209, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17556264, Length=302, Percent_Identity=28.476821192053, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=277, Percent_Identity=29.9638989169675, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=352, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=236, Percent_Identity=30.9322033898305, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=197, Percent_Identity=32.48730964467, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=136, Percent_Identity=34.5588235294118, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=37.3205741626794, Blast_Score=250, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=474, Percent_Identity=28.0590717299578, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=240, Percent_Identity=31.25, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648113, Length=459, Percent_Identity=44.0087145969499, Blast_Score=334, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24644968, Length=497, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=26.4344262295082, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=26.4344262295082, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=26.4344262295082, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=26.4344262295082, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=35.3658536585366, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=35.3658536585366, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=35.3658536585366, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=28.9108910891089, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356731, Length=236, Percent_Identity=31.7796610169492, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648441, Length=175, Percent_Identity=36.5714285714286, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24648439, Length=175, Percent_Identity=36.5714285714286, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_MOOTA (Q2RGY4)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430848.1
- ProteinModelPortal:   Q2RGY4
- SMR:   Q2RGY4
- STRING:   Q2RGY4
- GeneID:   3831963
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2009
- NMPDR:   fig|264732.9.peg.2027
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- PhylomeDB:   Q2RGY4
- BioCyc:   MTHE264732:MOTH_2009-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52551; Mature: 52551

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELYYLTAHELSDLLNRKEISSEEATAAIIDRIEAVDGRVQAYLTRTAEQALEEARAVDA
CEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARARGETLGPLAGVPMALKDNLCTEGVRTTCSSRMLADWVPPYDATVVRRLKEAGAVMLG
HHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEE
KLNMDEFAMGSSTENSSFFPTRNPWDLERVPGGSSGGAVAAVAAGEAYFALGSDTGGSIR
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCC
QPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPITRDVTDCALVLEAIAGHDPLDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
ADLPVPDYRSALKPEVKGLKIGVPREYFGAGMEPEVAAIVRRAIAKLEELGAVCEETSLP
CCCCCCHHHHHCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
HTEYALPAYYLVAPAEASSNLARYDGVSYGLRVPGKDIIEMYMNTRSQGFGPEVKRRIML
CCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
GTYALSSGYYDAYYLKALKVRTLIRRDFETAFEKYDLLATPTSPTVAFRLGEKAGDPLAM
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCHHHH
YLSDLCTIPINMAGLPALSLPCGFSQGLPVGLQLIGRPFAEATLLQAAYAFEQSTEYHRR
HHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
RPELGVA
CCCCCCC
>Mature Secondary Structure
MELYYLTAHELSDLLNRKEISSEEATAAIIDRIEAVDGRVQAYLTRTAEQALEEARAVDA
CEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARARGETLGPLAGVPMALKDNLCTEGVRTTCSSRMLADWVPPYDATVVRRLKEAGAVMLG
HHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEE
KLNMDEFAMGSSTENSSFFPTRNPWDLERVPGGSSGGAVAAVAAGEAYFALGSDTGGSIR
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCC
QPASFCGVVGMKPTYGRVSRYGLVAFASSLDQIGPITRDVTDCALVLEAIAGHDPLDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
ADLPVPDYRSALKPEVKGLKIGVPREYFGAGMEPEVAAIVRRAIAKLEELGAVCEETSLP
CCCCCCHHHHHCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
HTEYALPAYYLVAPAEASSNLARYDGVSYGLRVPGKDIIEMYMNTRSQGFGPEVKRRIML
CCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
GTYALSSGYYDAYYLKALKVRTLIRRDFETAFEKYDLLATPTSPTVAFRLGEKAGDPLAM
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCCHHHH
YLSDLCTIPINMAGLPALSLPCGFSQGLPVGLQLIGRPFAEATLLQAAYAFEQSTEYHRR
HHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
RPELGVA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA