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The map label for this gene is nth [H]

Identifier: 83590725

GI number: 83590725

Start: 1955398

End: 1956099

Strand: Reverse

Name: nth [H]

Synonym: Moth_1891

Alternate gene names: 83590725

Gene position: 1956099-1955398 (Counterclockwise)

Preceding gene: 83590727

Following gene: 83590724

Centisome position: 74.41

GC content: 61.54

Gene sequence:

>702_bases
ATGATATCACGATATTTGGCTTATGTGAAGCAGATTGTGATTATCTTTGTCTGGGGGATAAAGGTTATGCAGAGGGACAG
GGTAGAAGCAATTTTGCATTTATTACGGGCTGCCTACCCGGGAGCCCGCTCCCGTTTGAATTTCCGTAACCCCTTCGAAC
TCCTGGTGGCGGCCATCCTCTCCGCCCAGACCACCGACGATCAAGTTAACAAGGTAACAGGCGAGCTTTTCCGGCGTTAT
CCCACGCCGGAGGTCCTGGCGGCAGCAGACCCGGAGGAGGTGGCTGCTTGTATAAAAAGCCTGGGTCTTTACCGGACGAA
GGCCGCCCACCTGGTCGCGGCCTGCCGCACCCTGGTCAGGGAGTATGGCGGCCGGGTTCCGGATAAACTTGAGGACCTCC
TGCGCCTCCACGGGGTGGGGCGGAAGGTGGCCAATGTGGTCCTGAGCAACGCCTTTGGCCGGGATGTAATCGCCGTTGAC
ACCCATGTCTTCCGGGTGGCCAACCGGTTGGGCCTGGCCCGGGCTGGCGATGTCCGGGAGACGGAAAGGCAGCTCATGGC
CGCCCTGCCGCCGGGCAGCCGCGGTGAAGCCCACCACCTGCTGATCTATCACGGCCGGGAGGTCTGCAGGGCGCGCAACC
CCCGCTGCCGGGATTGTACCCTGCGTAGTTACTGCCGGGAGGCGGGAGGGTTACCTGAGTGA

Upstream 100 bases:

>100_bases
GGCAGTACAGGCCTTCTCCGGTCCAGCGCACGGTGGCAGTAAAGGTGGTTTTAGCCATACTAGTCATCCCTTTCGTAAAT
AATATATTGCTACCTTGTCC

Downstream 100 bases:

>100_bases
TCCGGATCACAACAGTTTTAAAAAGGGCATCACACCAACCATCCATCCTTCGGGATAGAATATGGCTAGAAGGATTGATA
ATGGAAGGTGGTATTTATGA

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MISRYLAYVKQIVIIFVWGIKVMQRDRVEAILHLLRAAYPGARSRLNFRNPFELLVAAILSAQTTDDQVNKVTGELFRRY
PTPEVLAAADPEEVAACIKSLGLYRTKAAHLVAACRTLVREYGGRVPDKLEDLLRLHGVGRKVANVVLSNAFGRDVIAVD
THVFRVANRLGLARAGDVRETERQLMAALPPGSRGEAHHLLIYHGREVCRARNPRCRDCTLRSYCREAGGLPE

Sequences:

>Translated_233_residues
MISRYLAYVKQIVIIFVWGIKVMQRDRVEAILHLLRAAYPGARSRLNFRNPFELLVAAILSAQTTDDQVNKVTGELFRRY
PTPEVLAAADPEEVAACIKSLGLYRTKAAHLVAACRTLVREYGGRVPDKLEDLLRLHGVGRKVANVVLSNAFGRDVIAVD
THVFRVANRLGLARAGDVRETERQLMAALPPGSRGEAHHLLIYHGREVCRARNPRCRDCTLRSYCREAGGLPE
>Mature_233_residues
MISRYLAYVKQIVIIFVWGIKVMQRDRVEAILHLLRAAYPGARSRLNFRNPFELLVAAILSAQTTDDQVNKVTGELFRRY
PTPEVLAAADPEEVAACIKSLGLYRTKAAHLVAACRTLVREYGGRVPDKLEDLLRLHGVGRKVANVVLSNAFGRDVIAVD
THVFRVANRLGLARAGDVRETERQLMAALPPGSRGEAHHLLIYHGREVCRARNPRCRDCTLRSYCREAGGLPE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=183, Percent_Identity=31.6939890710383, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI6912520, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI190358497, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI115298648, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI115298650, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI115298654, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI115298652, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787920, Length=204, Percent_Identity=43.6274509803922, Blast_Score=174, Evalue=3e-45,
Organism=Escherichia coli, GI1789331, Length=182, Percent_Identity=25.8241758241758, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=35.3932584269663, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319304, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=27.0833333333333, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=31.1111111111111, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26051; Mature: 26051

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRYLAYVKQIVIIFVWGIKVMQRDRVEAILHLLRAAYPGARSRLNFRNPFELLVAAIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SAQTTDDQVNKVTGELFRRYPTPEVLAAADPEEVAACIKSLGLYRTKAAHLVAACRTLVR
HCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYGGRVPDKLEDLLRLHGVGRKVANVVLSNAFGRDVIAVDTHVFRVANRLGLARAGDVRE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
TERQLMAALPPGSRGEAHHLLIYHGREVCRARNPRCRDCTLRSYCREAGGLPE
HHHHHHHHCCCCCCCCCEEEEEECCHHHHHCCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MISRYLAYVKQIVIIFVWGIKVMQRDRVEAILHLLRAAYPGARSRLNFRNPFELLVAAIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SAQTTDDQVNKVTGELFRRYPTPEVLAAADPEEVAACIKSLGLYRTKAAHLVAACRTLVR
HCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYGGRVPDKLEDLLRLHGVGRKVANVVLSNAFGRDVIAVDTHVFRVANRLGLARAGDVRE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHH
TERQLMAALPPGSRGEAHHLLIYHGREVCRARNPRCRDCTLRSYCREAGGLPE
HHHHHHHHCCCCCCCCCEEEEEECCHHHHHCCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]