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The map label for this gene is pckA2

Identifier: 83590727

GI number: 83590727

Start: 1956721

End: 1958301

Strand: Reverse

Name: pckA2

Synonym: Moth_1893

Alternate gene names: 83590727

Gene position: 1958301-1956721 (Counterclockwise)

Preceding gene: 83590728

Following gene: 83590725

Centisome position: 74.49

GC content: 60.85

Gene sequence:

>1581_bases
GTGGGAAATACTTATGGCTTAGAGGGTTTAGGTCTTATCAATCCCGGTAATATCTATCGTAACCTCCCGGTGGCCCGCCT
GGTGGAAATGGCCCTGGCCCGGGGCGAGGGAGTCCTGGCTTCCAACGGGGCCCTGAGCGTCAGGACCGGGAAATACACTG
GTCGTTCCCCCAACGACAGGTTCATGGTGGATACTCCTTCCGTTCACGATACCATCAGCTGGGGCGCTGTTAACCAGCCA
CTGGAGGAGGCCAGGTTTGAGGCCCTGTTTCGCCGCCTGGCGGCCTACCTCCAGGGGCGGGACCTTTTTATCTTCGACGG
TTTTGTCGGCGCCGATCCCGCCTACCGGATGCCCATCCGGGTGGTCAACCAGTACGCCTGGCAGAACCTCTTCGTTCACC
AGCTCTTTGTCCGGCCTTCGGCGGCGGAACTGGAGGCCCACGAACCGCAGTTTACCGTCATCTGTGCCCCCGGTTTCCAG
GCCACTCCTGAGGTCGACGGTACCCGCTCGGAAGCCTTTGTCGTCCTGAATTTCGACCGGCGGCTGATTATCATCGGCGG
GACTTCCTACGCCGGGGAAATGAAGAAATCTATCTTCACCGTGATGAACTATCTTTTACCGGAGCGGGGCGTCTGTCCCA
TGCACTGCTCGGCCAACATGGGGCCGGCCGGGGATACGGCCCTCTTCTTCGGCCTCTCCGGGACCGGCAAGACCACCCTC
TCGGCCGACCCGGAACGGCGCCTTATCGGCGACGACGAACACGGCTGGTCGGATCATGGTATCTTTAACTTTGAAGGCGG
CTGTTATGCCAAGTGCATTAAACTCTCGGCCGAGCACGAACCCCAGATCTGGAACGCCATCCGCTTCGGCAGCGTCCTGG
AGAATGTGGCCGTCGACCCGGACTCCCGGGCCATAGATTACGATTGCGACGCCCTGACGGAGAATACCAGGGCAGCTTAC
CCGGTAGATTTCATTCCTAACGCCGTCATCCCCGGTATAGGCGGTCACCCGCGAACCGTTGTTTTCCTTACGGCCGACGC
CTTTGGCGTCATGCCGCCCATTGCCAAACTGACCCGGGAACAGGCCATGTATCACTTCCTTTCCGGTTACACCAGCAAGC
TGGCGGGCACAGAGCGGGGAATCACCGATCCCCAGGCCACCTTCTCCACCTGTTTCGGCGCGCCTTTCCTGCCCAGGTCG
CCCCGGGTCTATGCCGACCTTCTGGGGGAAAGGATAGCCAAACACGGGGCCAGCGTCTACCTGGTCAATACCGGTTGGAC
GGGGGGACCCTCCGGCATCGGCCGGCGCATGAGCCTGCCTTACACCAGGGCTATGGTCAGGGCGGCCATCAAGGGCGAGC
TGGAAGGGGTAGAGTTTGTCCCTGATCCGGTTTTCGGCATCCTGGTGCCTACCTCGTGCCCCGGAGTGCCGGCCGAGGTC
CTCAATCCCCGTCATACCTGGCAGGATAAAGAAAAATATGATGCCATGGCCCGCAAGCTGGCCGGTCTCTTTGCCGAGAA
CTTTACCAAATTCCGGGATGTACCGGAGGAGATCCGGGCAGCCGGGCCGGTTGTTCGGTAG

Upstream 100 bases:

>100_bases
AATAGGTGTACTGTATACATGCCGGGATTCTGTTGACCTTCCCTTGAGCTGTATGGTATATTGCAAAAGATCATACAGAG
GGAGAAGGAAGGGAGAACCA

Downstream 100 bases:

>100_bases
GTAAATGCGCGGAACCCCTTAAAGCGAAAAAGCCGGCTTAAAAACCGGCTTTTTCGCTGTATTTTTTACTGGCCTTTTAC
CCCATTATTTTCCTCCCTGT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase 2; PEPCK; Phosphoenolpyruvate carboxylase 2

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MGNTYGLEGLGLINPGNIYRNLPVARLVEMALARGEGVLASNGALSVRTGKYTGRSPNDRFMVDTPSVHDTISWGAVNQP
LEEARFEALFRRLAAYLQGRDLFIFDGFVGADPAYRMPIRVVNQYAWQNLFVHQLFVRPSAAELEAHEPQFTVICAPGFQ
ATPEVDGTRSEAFVVLNFDRRLIIIGGTSYAGEMKKSIFTVMNYLLPERGVCPMHCSANMGPAGDTALFFGLSGTGKTTL
SADPERRLIGDDEHGWSDHGIFNFEGGCYAKCIKLSAEHEPQIWNAIRFGSVLENVAVDPDSRAIDYDCDALTENTRAAY
PVDFIPNAVIPGIGGHPRTVVFLTADAFGVMPPIAKLTREQAMYHFLSGYTSKLAGTERGITDPQATFSTCFGAPFLPRS
PRVYADLLGERIAKHGASVYLVNTGWTGGPSGIGRRMSLPYTRAMVRAAIKGELEGVEFVPDPVFGILVPTSCPGVPAEV
LNPRHTWQDKEKYDAMARKLAGLFAENFTKFRDVPEEIRAAGPVVR

Sequences:

>Translated_526_residues
MGNTYGLEGLGLINPGNIYRNLPVARLVEMALARGEGVLASNGALSVRTGKYTGRSPNDRFMVDTPSVHDTISWGAVNQP
LEEARFEALFRRLAAYLQGRDLFIFDGFVGADPAYRMPIRVVNQYAWQNLFVHQLFVRPSAAELEAHEPQFTVICAPGFQ
ATPEVDGTRSEAFVVLNFDRRLIIIGGTSYAGEMKKSIFTVMNYLLPERGVCPMHCSANMGPAGDTALFFGLSGTGKTTL
SADPERRLIGDDEHGWSDHGIFNFEGGCYAKCIKLSAEHEPQIWNAIRFGSVLENVAVDPDSRAIDYDCDALTENTRAAY
PVDFIPNAVIPGIGGHPRTVVFLTADAFGVMPPIAKLTREQAMYHFLSGYTSKLAGTERGITDPQATFSTCFGAPFLPRS
PRVYADLLGERIAKHGASVYLVNTGWTGGPSGIGRRMSLPYTRAMVRAAIKGELEGVEFVPDPVFGILVPTSCPGVPAEV
LNPRHTWQDKEKYDAMARKLAGLFAENFTKFRDVPEEIRAAGPVVR
>Mature_525_residues
GNTYGLEGLGLINPGNIYRNLPVARLVEMALARGEGVLASNGALSVRTGKYTGRSPNDRFMVDTPSVHDTISWGAVNQPL
EEARFEALFRRLAAYLQGRDLFIFDGFVGADPAYRMPIRVVNQYAWQNLFVHQLFVRPSAAELEAHEPQFTVICAPGFQA
TPEVDGTRSEAFVVLNFDRRLIIIGGTSYAGEMKKSIFTVMNYLLPERGVCPMHCSANMGPAGDTALFFGLSGTGKTTLS
ADPERRLIGDDEHGWSDHGIFNFEGGCYAKCIKLSAEHEPQIWNAIRFGSVLENVAVDPDSRAIDYDCDALTENTRAAYP
VDFIPNAVIPGIGGHPRTVVFLTADAFGVMPPIAKLTREQAMYHFLSGYTSKLAGTERGITDPQATFSTCFGAPFLPRSP
RVYADLLGERIAKHGASVYLVNTGWTGGPSGIGRRMSLPYTRAMVRAAIKGELEGVEFVPDPVFGILVPTSCPGVPAEVL
NPRHTWQDKEKYDAMARKLAGLFAENFTKFRDVPEEIRAAGPVVR

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=497, Percent_Identity=49.8993963782696, Blast_Score=489, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6322950, Length=516, Percent_Identity=51.3565891472868, Blast_Score=509, Evalue=1e-145,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA2_MOOTA (Q2RH96)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430736.1
- ProteinModelPortal:   Q2RH96
- SMR:   Q2RH96
- STRING:   Q2RH96
- GeneID:   3831166
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1893
- NMPDR:   fig|264732.9.peg.1899
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   PMHASAN
- PhylomeDB:   Q2RH96
- ProtClustDB:   PRK09344
- BioCyc:   MTHE264732:MOTH_1893-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 57522; Mature: 57391

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNTYGLEGLGLINPGNIYRNLPVARLVEMALARGEGVLASNGALSVRTGKYTGRSPNDR
CCCCCCCCCCEECCCCCHHHCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCC
FMVDTPSVHDTISWGAVNQPLEEARFEALFRRLAAYLQGRDLFIFDGFVGADPAYRMPIR
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHH
VVNQYAWQNLFVHQLFVRPSAAELEAHEPQFTVICAPGFQATPEVDGTRSEAFVVLNFDR
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCC
RLIIIGGTSYAGEMKKSIFTVMNYLLPERGVCPMHCSANMGPAGDTALFFGLSGTGKTTL
EEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEE
SADPERRLIGDDEHGWSDHGIFNFEGGCYAKCIKLSAEHEPQIWNAIRFGSVLENVAVDP
CCCCCCCCCCCCCCCCCCCCEEEECCCCEEEHEEECCCCCCHHHHHHHHHHHHHHHCCCC
DSRAIDYDCDALTENTRAAYPVDFIPNAVIPGIGGHPRTVVFLTADAFGVMPPIAKLTRE
CCCEECCCCHHHCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
QAMYHFLSGYTSKLAGTERGITDPQATFSTCFGAPFLPRSPRVYADLLGERIAKHGASVY
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
LVNTGWTGGPSGIGRRMSLPYTRAMVRAAIKGELEGVEFVPDPVFGILVPTSCPGVPAEV
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCCHHH
LNPRHTWQDKEKYDAMARKLAGLFAENFTKFRDVPEEIRAAGPVVR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
>Mature Secondary Structure 
GNTYGLEGLGLINPGNIYRNLPVARLVEMALARGEGVLASNGALSVRTGKYTGRSPNDR
CCCCCCCCCEECCCCCHHHCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCC
FMVDTPSVHDTISWGAVNQPLEEARFEALFRRLAAYLQGRDLFIFDGFVGADPAYRMPIR
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHH
VVNQYAWQNLFVHQLFVRPSAAELEAHEPQFTVICAPGFQATPEVDGTRSEAFVVLNFDR
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCC
RLIIIGGTSYAGEMKKSIFTVMNYLLPERGVCPMHCSANMGPAGDTALFFGLSGTGKTTL
EEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEE
SADPERRLIGDDEHGWSDHGIFNFEGGCYAKCIKLSAEHEPQIWNAIRFGSVLENVAVDP
CCCCCCCCCCCCCCCCCCCCEEEECCCCEEEHEEECCCCCCHHHHHHHHHHHHHHHCCCC
DSRAIDYDCDALTENTRAAYPVDFIPNAVIPGIGGHPRTVVFLTADAFGVMPPIAKLTRE
CCCEECCCCHHHCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
QAMYHFLSGYTSKLAGTERGITDPQATFSTCFGAPFLPRSPRVYADLLGERIAKHGASVY
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
LVNTGWTGGPSGIGRRMSLPYTRAMVRAAIKGELEGVEFVPDPVFGILVPTSCPGVPAEV
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCCHHH
LNPRHTWQDKEKYDAMARKLAGLFAENFTKFRDVPEEIRAAGPVVR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA