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The map label for this gene is pfkA [H]

Identifier: 83590702

GI number: 83590702

Start: 1931616

End: 1932575

Strand: Reverse

Name: pfkA [H]

Synonym: Moth_1868

Alternate gene names: 83590702

Gene position: 1932575-1931616 (Counterclockwise)

Preceding gene: 83590703

Following gene: 83590701

Centisome position: 73.52

GC content: 61.35

Gene sequence:

>960_bases
TTGCGCACTATCGGTGTTTTGACCAGCGGTGGTGATGCCCCGGGAATGAATGCAGCTATCAGGGCGGTAGTCCGCCAGGC
TGCCGCCCTGAAGATAGACGTGATCGGCATTTCCCGGGGGTACGCCGGGTTGATCCAGGGCGATTTTCGCCGCCTGAATA
CCGGTTCGGTAGCCGGGATTATCCACCGGGGCGGGACCATCTTGCTGACGGCCCGGTCGGAGGAGTTCCGCACCGAAGCC
GGCCGGGCCACGGCCCTGGATAACCTGCACCGGGAAGGCATTGAAGGTCTCGTAGTCATCGGTGGAGACGGGTCCTTCCA
CGGCGCTGTTCACCTGGCCAAAAAGGGCCTGCCGGTAATCGGCATCCCCGGGACCATTGATAACGACATAGCCGGCACCG
ATTACACCATCGGCTTTGATACGGCCGTTAATACGGCCGTAGAGGCCATCAGCCGGATCCGGGATACGGCGACTTCCCAT
GAACGCATTTTTATCATCGAAGTTATGGGGCGTCGCTCAGGCCAGATTGCCCTGGCGGCGGGGATAGCCGGGGGCGCCGA
ATCTATTCTCATCCCCGAGTACCCCGTTAACTACGACCGGGTGGTGGAGAAATTAGAACAGGGCCGCCACCGGGGTAAAC
TCCATAGTATTATCGTCGTAGCCGAAGGGGTGGGCAGTGCCCTGGAGGTAAGCGAAGAAATAGCCCGGCGAACCAGGCTG
GAGAGCCGCGTGACCATCCTGGGCCATATTCAGCGGGGTGGAGCGCCTACGGCTTTTGATTGTATGCTGGCCAGCCGGCT
GGGCGCCCGGGCGGTGGACCTCCTGGCCGACGGCGCCAGCAGCCGCATGGTGGGCATCGCCGCCAACGAGCTGGTGGACC
GGGACCTGGAGACGGTTCTCCGGGAAAAGAAGAGTATCGATCCTGAACTGTACCGTTTAGCCGAAGTACTGGCACTATAA

Upstream 100 bases:

>100_bases
GAGGACCTGGCTGGGTATGAGGTCCTGGTGCCCGAATCCGAGGCTGAGGAAGCCAATGAAATAATCAGTGCGGCCCTGAC
ACGCTAGAGGAGGGAAATAA

Downstream 100 bases:

>100_bases
ATAGGCCAGCGAGGGGGCAAATATGCGGCACACGAAGATTGTCTGTACCATGGGCCCGGCCAGCGAGCGGGTCGAGGTAA
TCAAGGCTATGATCCGGGCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRTIGVLTSGGDAPGMNAAIRAVVRQAAALKIDVIGISRGYAGLIQGDFRRLNTGSVAGIIHRGGTILLTARSEEFRTEA
GRATALDNLHREGIEGLVVIGGDGSFHGAVHLAKKGLPVIGIPGTIDNDIAGTDYTIGFDTAVNTAVEAISRIRDTATSH
ERIFIIEVMGRRSGQIALAAGIAGGAESILIPEYPVNYDRVVEKLEQGRHRGKLHSIIVVAEGVGSALEVSEEIARRTRL
ESRVTILGHIQRGGAPTAFDCMLASRLGARAVDLLADGASSRMVGIAANELVDRDLETVLREKKSIDPELYRLAEVLAL

Sequences:

>Translated_319_residues
MRTIGVLTSGGDAPGMNAAIRAVVRQAAALKIDVIGISRGYAGLIQGDFRRLNTGSVAGIIHRGGTILLTARSEEFRTEA
GRATALDNLHREGIEGLVVIGGDGSFHGAVHLAKKGLPVIGIPGTIDNDIAGTDYTIGFDTAVNTAVEAISRIRDTATSH
ERIFIIEVMGRRSGQIALAAGIAGGAESILIPEYPVNYDRVVEKLEQGRHRGKLHSIIVVAEGVGSALEVSEEIARRTRL
ESRVTILGHIQRGGAPTAFDCMLASRLGARAVDLLADGASSRMVGIAANELVDRDLETVLREKKSIDPELYRLAEVLAL
>Mature_319_residues
MRTIGVLTSGGDAPGMNAAIRAVVRQAAALKIDVIGISRGYAGLIQGDFRRLNTGSVAGIIHRGGTILLTARSEEFRTEA
GRATALDNLHREGIEGLVVIGGDGSFHGAVHLAKKGLPVIGIPGTIDNDIAGTDYTIGFDTAVNTAVEAISRIRDTATSH
ERIFIIEVMGRRSGQIALAAGIAGGAESILIPEYPVNYDRVVEKLEQGRHRGKLHSIIVVAEGVGSALEVSEEIARRTRL
ESRVTILGHIQRGGAPTAFDCMLASRLGARAVDLLADGASSRMVGIAANELVDRDLETVLREKKSIDPELYRLAEVLAL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=306, Percent_Identity=42.483660130719, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI48762920, Length=347, Percent_Identity=38.9048991354467, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI266453619, Length=351, Percent_Identity=37.8917378917379, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI266453768, Length=351, Percent_Identity=37.8917378917379, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI266453748, Length=351, Percent_Identity=37.8917378917379, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI4505749, Length=351, Percent_Identity=37.8917378917379, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI1790350, Length=304, Percent_Identity=51.3157894736842, Blast_Score=302, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI25147584, Length=306, Percent_Identity=43.1372549019608, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=38.1410256410256, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI72003601, Length=293, Percent_Identity=30.7167235494881, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=38.3720930232558, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=35.6521739130435, Blast_Score=185, Evalue=9e-48,
Organism=Drosophila melanogaster, GI24652337, Length=303, Percent_Identity=44.2244224422442, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI28573326, Length=303, Percent_Identity=44.2244224422442, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI17647809, Length=303, Percent_Identity=44.2244224422442, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 33751; Mature: 33751

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIGVLTSGGDAPGMNAAIRAVVRQAAALKIDVIGISRGYAGLIQGDFRRLNTGSVAGI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCEECCCCCEEEE
IHRGGTILLTARSEEFRTEAGRATALDNLHREGIEGLVVIGGDGSFHGAVHLAKKGLPVI
EECCCEEEEEECCHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCHHEEHHHCCCCEE
GIPGTIDNDIAGTDYTIGFDTAVNTAVEAISRIRDTATSHERIFIIEVMGRRSGQIALAA
ECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEE
GIAGGAESILIPEYPVNYDRVVEKLEQGRHRGKLHSIIVVAEGVGSALEVSEEIARRTRL
ECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
ESRVTILGHIQRGGAPTAFDCMLASRLGARAVDLLADGASSRMVGIAANELVDRDLETVL
HHHEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
REKKSIDPELYRLAEVLAL
HHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRTIGVLTSGGDAPGMNAAIRAVVRQAAALKIDVIGISRGYAGLIQGDFRRLNTGSVAGI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCEECCCCCEEEE
IHRGGTILLTARSEEFRTEAGRATALDNLHREGIEGLVVIGGDGSFHGAVHLAKKGLPVI
EECCCEEEEEECCHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCHHEEHHHCCCCEE
GIPGTIDNDIAGTDYTIGFDTAVNTAVEAISRIRDTATSHERIFIIEVMGRRSGQIALAA
ECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEE
GIAGGAESILIPEYPVNYDRVVEKLEQGRHRGKLHSIIVVAEGVGSALEVSEEIARRTRL
ECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
ESRVTILGHIQRGGAPTAFDCMLASRLGARAVDLLADGASSRMVGIAANELVDRDLETVL
HHHEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
REKKSIDPELYRLAEVLAL
HHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]