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The map label for this gene is pyk [H]

Identifier: 83590701

GI number: 83590701

Start: 1929845

End: 1931593

Strand: Reverse

Name: pyk [H]

Synonym: Moth_1867

Alternate gene names: 83590701

Gene position: 1931593-1929845 (Counterclockwise)

Preceding gene: 83590702

Following gene: 83590700

Centisome position: 73.48

GC content: 62.38

Gene sequence:

>1749_bases
ATGCGGCACACGAAGATTGTCTGTACCATGGGCCCGGCCAGCGAGCGGGTCGAGGTAATCAAGGCTATGATCCGGGCGGG
GATGAACGTAGCCCGGTTCAATTTTTCCCATGGCAGCCACGCCGAGCACGGGGCGCGGATGGCTGCCGTGCGCCAGGCGG
CAGCTGAACTGGGCGCCAGGGTAGCGTTAATGCTGGATAATAAGGGGCCCGAAATTCGCCTGGGAGAGATCCAGGGCGAG
GTCACCCTGAAGGACGGCGACCAGGTGACCCTGACCACAGAACCTATTATTGGTGACGCCAGGCGTTTGCCGGTGAGCTT
TGCCGGTCTGCCGGGGGACGTCCGGCCGGGCCAGATCATTCTCCTGGACGACGGCCTGGTGGAGCTGGAGGTCCTGGCGA
CCACCGCCACCGAGATTCACTGCCGCGTCCGTCACGGCGATGTTATTTCCAGCCATAAGGGCGTCAACGTCCCCGGGGCC
GAGATCAGCCTGCCTCCTTTTACCGAGCAGGATATTAAAGACCTTGAGTTCGGCCTCCAGCAGGGGATAGATTTTATCGC
CCTCTCCTTTGTCCGGACGGCCGGGGATGTCCTGGCAGTACGCCGGGAGCTTGAGAAGCGCAACGCCAGGGTAGCCATTA
TCGCCAAGATAGAAAACCATGCCGGGGTCAATAACATCCACGAGATCCTTGAGGTGGCCGACGGGGTCATGGTGGCCCGG
GGTGACCTGGGGGTAGAGATCCCCGTGGAAGAGGTCCCCCTGGTGCAGAAAAAGATTATCGAGGCGTGTAACCTGGCCGG
CAAGCCGGTTATCACGGCCACCCAGATGCTGGAGTCTATGATTCATAACCCGCGGCCGACCCGGGCCGAAGCCAGCGATG
TGGCCAATGCCATCTTTGACGGAACGGATGCCATTATGCTCTCCGGGGAAACGGCTACGGGCCGTTATCCGGTAGAGGCT
GTGGCGACCATGGCCCGCATCGCCCGCCGGGCCGAGAGGGGTTTGCCCTATGGTGACCTGTTGACGAAAAAGGGTCTGGC
TGCCGAGCGGACGGCCACCGATGCCATCAGCCACGCGAGCTGCACCATTGCCTATGAACTCGACGCCGCCGCCATTATCA
CCCCCACGGCTTCCGGTTCCACCGCCCGCCGGGTGGCCAAATACCGTCCCCGGGCGCCTATCCTGGCCACCAGCCCCAAC
GAGAAGGTTTTGAACCAGCTCTGCCTGGTCTGGGGGGTTGAACCCCTCCTGGTGGAGCCGACAAGCGGCACCGACGAAAT
GGTTAATGCCGCGGTGGCGGCGGCCATACTCTCCGGCCGGGTGAAACAGGGCGACCTGGTGGTTATTACTGCCGGCGTGC
CTGCCGGTGTTCCGGGTACCACCAACCTCCTCAAGGTCCACATCGTCGGCGAGGTCCTGGTGCGGGGACGGGGGATCGGC
AAAGAAGTAACCAGTGGCCCGGTCCGGCTGGTAAAGACTGCTGCTGACGCCGTGGCAAGGGTCAAAAAAGGCGACATTCT
GGTGACCACTGAGACCGGCCCTGAATTCCTGCCAGCTATGGAAAGGGCGGCAGCAGTAATTACGGAAACCGGGGGGCTGA
GTTCCCATGCCGCGGTAACCGGCCTGAGCCTGGGTATACCGGTAGTCGTCGGGGCAAAGGGGGCCACTGAAAAGCTAACC
GATGATCTGGTCGTAACCATAGACGTTGTCCGCGGCCTGGTCTACCGCGGTCAGACGCGGGTGTTGTGA

Upstream 100 bases:

>100_bases
GACCTGGAGACGGTTCTCCGGGAAAAGAAGAGTATCGATCCTGAACTGTACCGTTTAGCCGAAGTACTGGCACTATAAAT
AGGCCAGCGAGGGGGCAAAT

Downstream 100 bases:

>100_bases
GGCAAAAAACTTGGAGGAGGAGAAACAATGGCTGATTTAGACAAGTCCCTCATGGTCAAAAGGTTGACGGAAGTTCTCCA
CCTGGATACGGGCATCGTGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MRHTKIVCTMGPASERVEVIKAMIRAGMNVARFNFSHGSHAEHGARMAAVRQAAAELGARVALMLDNKGPEIRLGEIQGE
VTLKDGDQVTLTTEPIIGDARRLPVSFAGLPGDVRPGQIILLDDGLVELEVLATTATEIHCRVRHGDVISSHKGVNVPGA
EISLPPFTEQDIKDLEFGLQQGIDFIALSFVRTAGDVLAVRRELEKRNARVAIIAKIENHAGVNNIHEILEVADGVMVAR
GDLGVEIPVEEVPLVQKKIIEACNLAGKPVITATQMLESMIHNPRPTRAEASDVANAIFDGTDAIMLSGETATGRYPVEA
VATMARIARRAERGLPYGDLLTKKGLAAERTATDAISHASCTIAYELDAAAIITPTASGSTARRVAKYRPRAPILATSPN
EKVLNQLCLVWGVEPLLVEPTSGTDEMVNAAVAAAILSGRVKQGDLVVITAGVPAGVPGTTNLLKVHIVGEVLVRGRGIG
KEVTSGPVRLVKTAADAVARVKKGDILVTTETGPEFLPAMERAAAVITETGGLSSHAAVTGLSLGIPVVVGAKGATEKLT
DDLVVTIDVVRGLVYRGQTRVL

Sequences:

>Translated_582_residues
MRHTKIVCTMGPASERVEVIKAMIRAGMNVARFNFSHGSHAEHGARMAAVRQAAAELGARVALMLDNKGPEIRLGEIQGE
VTLKDGDQVTLTTEPIIGDARRLPVSFAGLPGDVRPGQIILLDDGLVELEVLATTATEIHCRVRHGDVISSHKGVNVPGA
EISLPPFTEQDIKDLEFGLQQGIDFIALSFVRTAGDVLAVRRELEKRNARVAIIAKIENHAGVNNIHEILEVADGVMVAR
GDLGVEIPVEEVPLVQKKIIEACNLAGKPVITATQMLESMIHNPRPTRAEASDVANAIFDGTDAIMLSGETATGRYPVEA
VATMARIARRAERGLPYGDLLTKKGLAAERTATDAISHASCTIAYELDAAAIITPTASGSTARRVAKYRPRAPILATSPN
EKVLNQLCLVWGVEPLLVEPTSGTDEMVNAAVAAAILSGRVKQGDLVVITAGVPAGVPGTTNLLKVHIVGEVLVRGRGIG
KEVTSGPVRLVKTAADAVARVKKGDILVTTETGPEFLPAMERAAAVITETGGLSSHAAVTGLSLGIPVVVGAKGATEKLT
DDLVVTIDVVRGLVYRGQTRVL
>Mature_582_residues
MRHTKIVCTMGPASERVEVIKAMIRAGMNVARFNFSHGSHAEHGARMAAVRQAAAELGARVALMLDNKGPEIRLGEIQGE
VTLKDGDQVTLTTEPIIGDARRLPVSFAGLPGDVRPGQIILLDDGLVELEVLATTATEIHCRVRHGDVISSHKGVNVPGA
EISLPPFTEQDIKDLEFGLQQGIDFIALSFVRTAGDVLAVRRELEKRNARVAIIAKIENHAGVNNIHEILEVADGVMVAR
GDLGVEIPVEEVPLVQKKIIEACNLAGKPVITATQMLESMIHNPRPTRAEASDVANAIFDGTDAIMLSGETATGRYPVEA
VATMARIARRAERGLPYGDLLTKKGLAAERTATDAISHASCTIAYELDAAAIITPTASGSTARRVAKYRPRAPILATSPN
EKVLNQLCLVWGVEPLLVEPTSGTDEMVNAAVAAAILSGRVKQGDLVVITAGVPAGVPGTTNLLKVHIVGEVLVRGRGIG
KEVTSGPVRLVKTAADAVARVKKGDILVTTETGPEFLPAMERAAAVITETGGLSSHAAVTGLSLGIPVVVGAKGATEKLT
DDLVVTIDVVRGLVYRGQTRVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=486, Percent_Identity=46.2962962962963, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI10835121, Length=486, Percent_Identity=46.2962962962963, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=45.0617283950617, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=45.0617283950617, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI33286418, Length=486, Percent_Identity=44.8559670781893, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=46.3414634146341, Blast_Score=240, Evalue=3e-63,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=46.3414634146341, Blast_Score=240, Evalue=3e-63,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=42.5531914893617, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=42.5531914893617, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=41.1214953271028, Blast_Score=155, Evalue=9e-38,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=47.6793248945148, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=40.3765690376569, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1787994, Length=107, Percent_Identity=38.3177570093458, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17544584, Length=481, Percent_Identity=43.6590436590437, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=42.8865979381443, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=42.8865979381443, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=42.8865979381443, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=42.8865979381443, Blast_Score=343, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6324923, Length=494, Percent_Identity=41.7004048582996, Blast_Score=343, Evalue=5e-95,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=41.9753086419753, Blast_Score=342, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=43.558282208589, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=43.558282208589, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=46.2102689486553, Blast_Score=341, Evalue=9e-94,
Organism=Drosophila melanogaster, GI24581235, Length=487, Percent_Identity=34.2915811088296, Blast_Score=279, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24646914, Length=302, Percent_Identity=41.7218543046358, Blast_Score=195, Evalue=6e-50,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 61373; Mature: 61373

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHTKIVCTMGPASERVEVIKAMIRAGMNVARFNFSHGSHAEHGARMAAVRQAAAELGAR
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCE
VALMLDNKGPEIRLGEIQGEVTLKDGDQVTLTTEPIIGDARRLPVSFAGLPGDVRPGQII
EEEEECCCCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
LLDDGLVELEVLATTATEIHCRVRHGDVISSHKGVNVPGAEISLPPFTEQDIKDLEFGLQ
EEECCCEEEEEEEEEEEEEEEEEECCCEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHH
QGIDFIALSFVRTAGDVLAVRRELEKRNARVAIIAKIENHAGVNNIHEILEVADGVMVAR
HCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCEEEEE
GDLGVEIPVEEVPLVQKKIIEACNLAGKPVITATQMLESMIHNPRPTRAEASDVANAIFD
CCCCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
GTDAIMLSGETATGRYPVEAVATMARIARRAERGLPYGDLLTKKGLAAERTATDAISHAS
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCE
CTIAYELDAAAIITPTASGSTARRVAKYRPRAPILATSPNEKVLNQLCLVWGVEPLLVEP
EEEEEEECCEEEEECCCCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEC
TSGTDEMVNAAVAAAILSGRVKQGDLVVITAGVPAGVPGTTNLLKVHIVGEVLVRGRGIG
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEHHHCCCCCC
KEVTSGPVRLVKTAADAVARVKKGDILVTTETGPEFLPAMERAAAVITETGGLSSHAAVT
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHEEEEECCCCCCCHHHE
GLSLGIPVVVGAKGATEKLTDDLVVTIDVVRGLVYRGQTRVL
EEECCCCEEEECCCCCHHHCCCCEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRHTKIVCTMGPASERVEVIKAMIRAGMNVARFNFSHGSHAEHGARMAAVRQAAAELGAR
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCE
VALMLDNKGPEIRLGEIQGEVTLKDGDQVTLTTEPIIGDARRLPVSFAGLPGDVRPGQII
EEEEECCCCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
LLDDGLVELEVLATTATEIHCRVRHGDVISSHKGVNVPGAEISLPPFTEQDIKDLEFGLQ
EEECCCEEEEEEEEEEEEEEEEEECCCEECCCCCCCCCCCCEECCCCCHHHHHHHHHHHH
QGIDFIALSFVRTAGDVLAVRRELEKRNARVAIIAKIENHAGVNNIHEILEVADGVMVAR
HCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCEEEEE
GDLGVEIPVEEVPLVQKKIIEACNLAGKPVITATQMLESMIHNPRPTRAEASDVANAIFD
CCCCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
GTDAIMLSGETATGRYPVEAVATMARIARRAERGLPYGDLLTKKGLAAERTATDAISHAS
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCE
CTIAYELDAAAIITPTASGSTARRVAKYRPRAPILATSPNEKVLNQLCLVWGVEPLLVEP
EEEEEEECCEEEEECCCCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEC
TSGTDEMVNAAVAAAILSGRVKQGDLVVITAGVPAGVPGTTNLLKVHIVGEVLVRGRGIG
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEEHHHCCCCCC
KEVTSGPVRLVKTAADAVARVKKGDILVTTETGPEFLPAMERAAAVITETGGLSSHAAVT
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHEEEEECCCCCCCHHHE
GLSLGIPVVVGAKGATEKLTDDLVVTIDVVRGLVYRGQTRVL
EEECCCCEEEECCCCCHHHCCCCEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]