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The map label for this gene is polA [H]

Identifier: 83590675

GI number: 83590675

Start: 1897551

End: 1900208

Strand: Reverse

Name: polA [H]

Synonym: Moth_1841

Alternate gene names: 83590675

Gene position: 1900208-1897551 (Counterclockwise)

Preceding gene: 83590676

Following gene: 83590674

Centisome position: 72.28

GC content: 61.74

Gene sequence:

>2658_bases
ATGCCCACCAAAACCGCTAGACTTCTCCTGGTCGACGGCAACAGCGTCATTCACCGGGCTTTTCATGCCCTGCCCCCCCT
GCAGACCAGGGAAGGTATTCGCACCAATGCCGTCTACGGCTTTGCAACCATGTTGCAAAAGGCCAGGGAGATGTTTAAAC
CCGATTATATTATTGTCGCCTTTGACCATAGTAAGGTTACCTTTCGTAACGAACTGTACGATGAATATAAAGGAACCCGG
CCGGAGACCGACCCTGAGCTCAGGCCCCAGTTTGCCCTGGTCAAACGCCTCCTGGCAGCCTGGAACCTGGCCAGTTGTGA
GGTTGAGGGCTACGAAGCCGACGACCTTATCGGCACCTTAAGCCGCCAGGGCGCCGCCACGGGCCTGGAAGTCCTCATCC
TCACCGGGGACCGCGACGCCCTGCAACTAGTGGGAGAACGGGTGAAGGTCCTCCTTATGCGGCGCGGCCTTTCCCAGGTA
GAGGTCATCGACCGGGAAGCAATCAAGAAAAACTATGGCCTGGAACCGGAGCAGCTCATTGACGTCAAGGCCCTGATGGG
CGACGCCTCGGATAATATTCCCGGCGTACCGGGGGTGGGGGAGAAAACGGCCGTCCAGCTCGTCCGCCAGTACGGCGACC
TGGAAGGAGTCCTGGCCCACAGCGGGGAGATAAAAGGACGCCGGGTAGCGGAGAACCTGGTGACCTTCGCCGACCAGGCG
CGCCTGGCCCGGCGCCTGGCCACAATTGACTGCCAGGCTCCTGTGACCCTCGACCTGGCAGGGTGCTGCAACCAGTCGCC
GGACTACGAGGCCGTCCTGGCCCTTTATAAAGAACTGGAGTTCCACAGTCTGGTCAAGGACGTCCTCAGGGCCATGGAAC
AGGAAGGCAAGAAGGCCTCGCAAGAAACGACTGCTGTCCGGGGACTGTCGCTGCCGGACCCACTCACCCTGGACAGCCTG
GAAGAACTGGCGGAACTGGTGGCCAGGTTGGTCGGGAAGACGGATGTGGCCCTGGAATTGATTCTTAATAATCCCAGCTA
CCTGGAGGCCGCAGCCGTCGCCGTAGGGCTGGCCTGGGAGGATGGGGTGGCGGTCCTGGGTACAGCCGGGATAGAGCCTG
CCGCCCTGGCCGGTACCCTGGAACCATTGCTCCGGGTCAACCCCATCTTCCACGACGCCAAAAGGGCCCTGGTCTGGTTC
AGCAATGCCGGGGCGGGGGTTGCCGACCCCGGCGGCGATACTATGGTGGCCGGTTATCTCTTAAACCCCTCGGCCTCGCG
CCATGACCTGCCCGAACTCTGCCTGGAACACTTGAACCTGGCCCTGGTGGAGGGGGATTCCCCGCAACTGGCAGCGGCCC
GGCGGGCCGCTGTCATCAGGTTGCTCCACCGGGAACTGGCCAGTAAACTCCAGGTTGCCGGGATGGAGAACCTCTACCGG
CGGGTGGAGTTGCCCCTGACCCGGGTCCTAGGGGCCATGGAAAGCTACGGCGTGGCCGTCAACATGGAAACCCTGGATCT
CATGGGAATAGAGCTGGAGGGTGGGCTGGCCGCCCTCACAGAGGCCATCTACGAGCTGGCCGGGGAAGAGTTTAACCTGA
ATTCCCCCAAGCAGCTGGCCGTTATACTCTTTGAAAAGCTCGGACTGCCGCCGGTAAAGCGTACCAAGACCGGTTTTTCT
ACCGATGCCGCCGTCCTGGAGGAACTGGCCTGCAGGCACCCCATAGCCGCCAAACTGGTCGAGTACCGCCAGCTGGCCAA
ACTGAAATCGACCTATGTGGATGGTCTTAAACCCCTGGTCAATCCCCGCACGGGGAGCCTGCATACCAGCTTTAACCAGA
CGGTGACGGCCACCGGCCGCCTCTCCAGCAGCGAGCCCAATCTCCAGAATATCCCGGTGCGCCTGGAACTGGGCCGGCGC
CTGCGCAAGGCCTTTGTACCCCACGGTCCCGGCCGGTTGCTCCTGGCCGCCGACTACTCCCAGATCGAGCTGCGCATCCT
GGCCCATATTTCCGGCGATGAAGCCATGATTGCAGCCTTTCGCCGGGGGGAGGATATCCACGCCCGGACTGCGGCCGAGG
TCTTCGGCGTCCCCCTTGGTGAAGTGACGCCGGCTATGCGCCGCAGCGCCAAGGCGGTGAACTTCGGTATTGTCTACGGC
ATCAGCGACTACGGCCTGAGCCGGGATCTGGGGATAAGCCGCAGCGAAGCCCACGATTATATCGAACGTTACTTTCGGCG
TTACCGGGGCGTCAAGGCCTATCTGGAGGAGATCGTAGCCCGGGCGCGGCAGGAGGGCTATGTCACCACCCTCCTGGGCC
GCCGCCGTTATCTGCCGGACCTCTTTAGCTCCAACCGCAATGTCCGCAGCTTTGGCGAGCGCACGGCCATGAATACGCCT
ATCCAGGGCACGGCCGCCGACATCATCAAGATGGCCATGGTGAAAATCTTTCGCCTCCTGGAGGCACAATACCCGGCCGC
GCGTATGATCCTCCAGGTCCACGACGAACTCATCTTTGATGTCCCGGATGACGACCTGCCGGCCGTGGCCGGCCTGGTCA
AGGATACCATGGAGCATACCCTGGAACTCCAGGTCCCCCTCCAGGTAGATTTAAAGGCCGGGCCCAACTGGTATGACCTG
GAGCCGTATAAGGAGTAA

Upstream 100 bases:

>100_bases
GGTTTTAAAATTTCTGTTTGCTGATCCCCGCCAGTTAATTGCTTCCGGCAGGGGAAAAAGATAAAATATAAGCAGTTCTA
AGCTAAAAGAAGGGTGATTA

Downstream 100 bases:

>100_bases
AAGGAAAATGCCCGAACTACCCGAAGTAGAAACCATTAAACGGACACTGACTCCCTGCTTACGGGAGCAAAAAATCGCCA
GGGTGGAGGTCTACCACCCT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 885; Mature: 884

Protein sequence:

>885_residues
MPTKTARLLLVDGNSVIHRAFHALPPLQTREGIRTNAVYGFATMLQKAREMFKPDYIIVAFDHSKVTFRNELYDEYKGTR
PETDPELRPQFALVKRLLAAWNLASCEVEGYEADDLIGTLSRQGAATGLEVLILTGDRDALQLVGERVKVLLMRRGLSQV
EVIDREAIKKNYGLEPEQLIDVKALMGDASDNIPGVPGVGEKTAVQLVRQYGDLEGVLAHSGEIKGRRVAENLVTFADQA
RLARRLATIDCQAPVTLDLAGCCNQSPDYEAVLALYKELEFHSLVKDVLRAMEQEGKKASQETTAVRGLSLPDPLTLDSL
EELAELVARLVGKTDVALELILNNPSYLEAAAVAVGLAWEDGVAVLGTAGIEPAALAGTLEPLLRVNPIFHDAKRALVWF
SNAGAGVADPGGDTMVAGYLLNPSASRHDLPELCLEHLNLALVEGDSPQLAAARRAAVIRLLHRELASKLQVAGMENLYR
RVELPLTRVLGAMESYGVAVNMETLDLMGIELEGGLAALTEAIYELAGEEFNLNSPKQLAVILFEKLGLPPVKRTKTGFS
TDAAVLEELACRHPIAAKLVEYRQLAKLKSTYVDGLKPLVNPRTGSLHTSFNQTVTATGRLSSSEPNLQNIPVRLELGRR
LRKAFVPHGPGRLLLAADYSQIELRILAHISGDEAMIAAFRRGEDIHARTAAEVFGVPLGEVTPAMRRSAKAVNFGIVYG
ISDYGLSRDLGISRSEAHDYIERYFRRYRGVKAYLEEIVARARQEGYVTTLLGRRRYLPDLFSSNRNVRSFGERTAMNTP
IQGTAADIIKMAMVKIFRLLEAQYPAARMILQVHDELIFDVPDDDLPAVAGLVKDTMEHTLELQVPLQVDLKAGPNWYDL
EPYKE

Sequences:

>Translated_885_residues
MPTKTARLLLVDGNSVIHRAFHALPPLQTREGIRTNAVYGFATMLQKAREMFKPDYIIVAFDHSKVTFRNELYDEYKGTR
PETDPELRPQFALVKRLLAAWNLASCEVEGYEADDLIGTLSRQGAATGLEVLILTGDRDALQLVGERVKVLLMRRGLSQV
EVIDREAIKKNYGLEPEQLIDVKALMGDASDNIPGVPGVGEKTAVQLVRQYGDLEGVLAHSGEIKGRRVAENLVTFADQA
RLARRLATIDCQAPVTLDLAGCCNQSPDYEAVLALYKELEFHSLVKDVLRAMEQEGKKASQETTAVRGLSLPDPLTLDSL
EELAELVARLVGKTDVALELILNNPSYLEAAAVAVGLAWEDGVAVLGTAGIEPAALAGTLEPLLRVNPIFHDAKRALVWF
SNAGAGVADPGGDTMVAGYLLNPSASRHDLPELCLEHLNLALVEGDSPQLAAARRAAVIRLLHRELASKLQVAGMENLYR
RVELPLTRVLGAMESYGVAVNMETLDLMGIELEGGLAALTEAIYELAGEEFNLNSPKQLAVILFEKLGLPPVKRTKTGFS
TDAAVLEELACRHPIAAKLVEYRQLAKLKSTYVDGLKPLVNPRTGSLHTSFNQTVTATGRLSSSEPNLQNIPVRLELGRR
LRKAFVPHGPGRLLLAADYSQIELRILAHISGDEAMIAAFRRGEDIHARTAAEVFGVPLGEVTPAMRRSAKAVNFGIVYG
ISDYGLSRDLGISRSEAHDYIERYFRRYRGVKAYLEEIVARARQEGYVTTLLGRRRYLPDLFSSNRNVRSFGERTAMNTP
IQGTAADIIKMAMVKIFRLLEAQYPAARMILQVHDELIFDVPDDDLPAVAGLVKDTMEHTLELQVPLQVDLKAGPNWYDL
EPYKE
>Mature_884_residues
PTKTARLLLVDGNSVIHRAFHALPPLQTREGIRTNAVYGFATMLQKAREMFKPDYIIVAFDHSKVTFRNELYDEYKGTRP
ETDPELRPQFALVKRLLAAWNLASCEVEGYEADDLIGTLSRQGAATGLEVLILTGDRDALQLVGERVKVLLMRRGLSQVE
VIDREAIKKNYGLEPEQLIDVKALMGDASDNIPGVPGVGEKTAVQLVRQYGDLEGVLAHSGEIKGRRVAENLVTFADQAR
LARRLATIDCQAPVTLDLAGCCNQSPDYEAVLALYKELEFHSLVKDVLRAMEQEGKKASQETTAVRGLSLPDPLTLDSLE
ELAELVARLVGKTDVALELILNNPSYLEAAAVAVGLAWEDGVAVLGTAGIEPAALAGTLEPLLRVNPIFHDAKRALVWFS
NAGAGVADPGGDTMVAGYLLNPSASRHDLPELCLEHLNLALVEGDSPQLAAARRAAVIRLLHRELASKLQVAGMENLYRR
VELPLTRVLGAMESYGVAVNMETLDLMGIELEGGLAALTEAIYELAGEEFNLNSPKQLAVILFEKLGLPPVKRTKTGFST
DAAVLEELACRHPIAAKLVEYRQLAKLKSTYVDGLKPLVNPRTGSLHTSFNQTVTATGRLSSSEPNLQNIPVRLELGRRL
RKAFVPHGPGRLLLAADYSQIELRILAHISGDEAMIAAFRRGEDIHARTAAEVFGVPLGEVTPAMRRSAKAVNFGIVYGI
SDYGLSRDLGISRSEAHDYIERYFRRYRGVKAYLEEIVARARQEGYVTTLLGRRRYLPDLFSSNRNVRSFGERTAMNTPI
QGTAADIIKMAMVKIFRLLEAQYPAARMILQVHDELIFDVPDDDLPAVAGLVKDTMEHTLELQVPLQVDLKAGPNWYDLE
PYKE

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=534, Percent_Identity=35.0187265917603, Blast_Score=295, Evalue=1e-79,
Organism=Homo sapiens, GI139394648, Length=613, Percent_Identity=28.2218597063622, Blast_Score=239, Evalue=7e-63,
Organism=Escherichia coli, GI1790294, Length=922, Percent_Identity=41.4316702819957, Blast_Score=600, Evalue=1e-172,
Organism=Escherichia coli, GI226510970, Length=199, Percent_Identity=27.1356783919598, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI86563272, Length=395, Percent_Identity=30.126582278481, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17933644, Length=415, Percent_Identity=33.0120481927711, Blast_Score=222, Evalue=9e-58,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 97277; Mature: 97146

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTKTARLLLVDGNSVIHRAFHALPPLQTREGIRTNAVYGFATMLQKAREMFKPDYIIVA
CCCCCEEEEEECCCHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
FDHSKVTFRNELYDEYKGTRPETDPELRPQFALVKRLLAAWNLASCEVEGYEADDLIGTL
ECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHH
SRQGAATGLEVLILTGDRDALQLVGERVKVLLMRRGLSQVEVIDREAIKKNYGLEPEQLI
HHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
DVKALMGDASDNIPGVPGVGEKTAVQLVRQYGDLEGVLAHSGEIKGRRVAENLVTFADQA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
RLARRLATIDCQAPVTLDLAGCCNQSPDYEAVLALYKELEFHSLVKDVLRAMEQEGKKAS
HHHHHHHHCCCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QETTAVRGLSLPDPLTLDSLEELAELVARLVGKTDVALELILNNPSYLEAAAVAVGLAWE
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHEEEECC
DGVAVLGTAGIEPAALAGTLEPLLRVNPIFHDAKRALVWFSNAGAGVADPGGDTMVAGYL
CCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEE
LNPSASRHDLPELCLEHLNLALVEGDSPQLAAARRAAVIRLLHRELASKLQVAGMENLYR
ECCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
RVELPLTRVLGAMESYGVAVNMETLDLMGIELEGGLAALTEAIYELAGEEFNLNSPKQLA
HHHCCHHHHHHHHHHCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHH
VILFEKLGLPPVKRTKTGFSTDAAVLEELACRHPIAAKLVEYRQLAKLKSTYVDGLKPLV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NPRTGSLHTSFNQTVTATGRLSSSEPNLQNIPVRLELGRRLRKAFVPHGPGRLLLAADYS
CCCCCCEECCCCCEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCEEEEECCC
QIELRILAHISGDEAMIAAFRRGEDIHARTAAEVFGVPLGEVTPAMRRSAKAVNFGIVYG
EEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCHHHHHCCCCEEEEEEEE
ISDYGLSRDLGISRSEAHDYIERYFRRYRGVKAYLEEIVARARQEGYVTTLLGRRRYLPD
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
LFSSNRNVRSFGERTAMNTPIQGTAADIIKMAMVKIFRLLEAQYPAARMILQVHDELIFD
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEE
VPDDDLPAVAGLVKDTMEHTLELQVPLQVDLKAGPNWYDLEPYKE
CCCCCHHHHHHHHHHHHHHHEEEEECEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PTKTARLLLVDGNSVIHRAFHALPPLQTREGIRTNAVYGFATMLQKAREMFKPDYIIVA
CCCCEEEEEECCCHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
FDHSKVTFRNELYDEYKGTRPETDPELRPQFALVKRLLAAWNLASCEVEGYEADDLIGTL
ECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHH
SRQGAATGLEVLILTGDRDALQLVGERVKVLLMRRGLSQVEVIDREAIKKNYGLEPEQLI
HHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
DVKALMGDASDNIPGVPGVGEKTAVQLVRQYGDLEGVLAHSGEIKGRRVAENLVTFADQA
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
RLARRLATIDCQAPVTLDLAGCCNQSPDYEAVLALYKELEFHSLVKDVLRAMEQEGKKAS
HHHHHHHHCCCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QETTAVRGLSLPDPLTLDSLEELAELVARLVGKTDVALELILNNPSYLEAAAVAVGLAWE
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHEEEECC
DGVAVLGTAGIEPAALAGTLEPLLRVNPIFHDAKRALVWFSNAGAGVADPGGDTMVAGYL
CCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEE
LNPSASRHDLPELCLEHLNLALVEGDSPQLAAARRAAVIRLLHRELASKLQVAGMENLYR
ECCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
RVELPLTRVLGAMESYGVAVNMETLDLMGIELEGGLAALTEAIYELAGEEFNLNSPKQLA
HHHCCHHHHHHHHHHCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHH
VILFEKLGLPPVKRTKTGFSTDAAVLEELACRHPIAAKLVEYRQLAKLKSTYVDGLKPLV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
NPRTGSLHTSFNQTVTATGRLSSSEPNLQNIPVRLELGRRLRKAFVPHGPGRLLLAADYS
CCCCCCEECCCCCEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCEEEEECCC
QIELRILAHISGDEAMIAAFRRGEDIHARTAAEVFGVPLGEVTPAMRRSAKAVNFGIVYG
EEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCHHHHHCCCCEEEEEEEE
ISDYGLSRDLGISRSEAHDYIERYFRRYRGVKAYLEEIVARARQEGYVTTLLGRRRYLPD
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
LFSSNRNVRSFGERTAMNTPIQGTAADIIKMAMVKIFRLLEAQYPAARMILQVHDELIFD
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEE
VPDDDLPAVAGLVKDTMEHTLELQVPLQVDLKAGPNWYDLEPYKE
CCCCCHHHHHHHHHHHHHHHEEEEECEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]