Click here to switch to the map view.

The map label for this gene is cooF [H]

Identifier: 83590139

GI number: 83590139

Start: 1331037

End: 1331492

Strand: Reverse

Name: cooF [H]

Synonym: Moth_1291

Alternate gene names: 83590139

Gene position: 1331492-1331037 (Counterclockwise)

Preceding gene: 83590140

Following gene: 83590138

Centisome position: 50.65

GC content: 59.87

Gene sequence:

>456_bases
GTGAAAAAAGTATATGCCCGGCCGGCCTACTGTATGAACTGCCACCTGTGCGAAGTCAACTGTATCGTGGCCCACTCCCG
GAGCCATGACGTCTTCCGAGCCTATATGCGTGAGAATTTAAGGGGTACGGCCCGGGTAATTGTGGAAGAAAAGTTGCCCT
TGTCGGTAGCCGTCCAGTGCCGCCACTGCGCCGATCCCGCCTGTGTCGCCGGGTGCATCTCCGGGGCGATGATTAAAGAC
CCGGTAACAGGAATCGTCTACTGCCAAGAAGACAAATGCGTGGGTTGCTGGACCTGTGTGGCCAGCTGTCCCTTTGGGGC
CATCCGGCCCGGGGAGAAAAACGGCCGCCCGGTACCTTTAAAGTGCGACCTCTGCCGGGAAGAAGGCGAACCGGCCTGCG
TGGCCGGTTGCCCCAACCGGGCCCTGGTTTTTGAAGAGAGGAGGGCAGGGGCATGA

Upstream 100 bases:

>100_bases
ATCGCCTGCACCTCAGGGGCGTGGGGTTGAACGAAGTCGAGCTCAAGCTCCTGGGCGTTAAGCATGCCGGCGAAGCCATC
TGACGGGAGGGATCAAACCG

Downstream 100 bases:

>100_bases
ACTACGTCCTTATCGGCAATTCCGTAGCTGCTGTCAACGCCGTGGCCGGTATCCGGGAATACGACCGGGAAGGGGATATC
ACCATCGTGTCGGCGGAGAA

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: CO2; AH2

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MKKVYARPAYCMNCHLCEVNCIVAHSRSHDVFRAYMRENLRGTARVIVEEKLPLSVAVQCRHCADPACVAGCISGAMIKD
PVTGIVYCQEDKCVGCWTCVASCPFGAIRPGEKNGRPVPLKCDLCREEGEPACVAGCPNRALVFEERRAGA

Sequences:

>Translated_151_residues
MKKVYARPAYCMNCHLCEVNCIVAHSRSHDVFRAYMRENLRGTARVIVEEKLPLSVAVQCRHCADPACVAGCISGAMIKD
PVTGIVYCQEDKCVGCWTCVASCPFGAIRPGEKNGRPVPLKCDLCREEGEPACVAGCPNRALVFEERRAGA
>Mature_151_residues
MKKVYARPAYCMNCHLCEVNCIVAHSRSHDVFRAYMRENLRGTARVIVEEKLPLSVAVQCRHCADPACVAGCISGAMIKD
PVTGIVYCQEDKCVGCWTCVASCPFGAIRPGEKNGRPVPLKCDLCREEGEPACVAGCPNRALVFEERRAGA

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH). CooF is required in stoichiometric amounts in vitro for anchoring CODH to the membrane as well as

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=159, Percent_Identity=35.2201257861635, Blast_Score=83, Evalue=8e-18,
Organism=Escherichia coli, GI87082179, Length=151, Percent_Identity=35.7615894039735, Blast_Score=80, Evalue=5e-17,
Organism=Escherichia coli, GI1788811, Length=146, Percent_Identity=33.5616438356164, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI2367245, Length=141, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1789370, Length=108, Percent_Identity=37.037037037037, Blast_Score=70, Evalue=5e-14,
Organism=Escherichia coli, GI87082180, Length=148, Percent_Identity=34.4594594594595, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI1787122, Length=142, Percent_Identity=36.6197183098592, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1787872, Length=142, Percent_Identity=35.2112676056338, Blast_Score=67, Evalue=5e-13,
Organism=Escherichia coli, GI1787749, Length=93, Percent_Identity=38.7096774193548, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI2367345, Length=93, Percent_Identity=40.8602150537634, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1790326, Length=94, Percent_Identity=37.2340425531915, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.99.2

Molecular weight: Translated: 16478; Mature: 16478

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
13.9 %Cys+Met (Translated Protein)
11.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
13.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVYARPAYCMNCHLCEVNCIVAHSRSHDVFRAYMRENLRGTARVIVEEKLPLSVAVQC
CCCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEE
RHCADPACVAGCISGAMIKDPVTGIVYCQEDKCVGCWTCVASCPFGAIRPGEKNGRPVPL
CCCCCHHHHHHHHCCCEECCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCE
KCDLCREEGEPACVAGCPNRALVFEERRAGA
EEHHHHCCCCCEEEECCCCCEEEEECCCCCC
>Mature Secondary Structure
MKKVYARPAYCMNCHLCEVNCIVAHSRSHDVFRAYMRENLRGTARVIVEEKLPLSVAVQC
CCCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEE
RHCADPACVAGCISGAMIKDPVTGIVYCQEDKCVGCWTCVASCPFGAIRPGEKNGRPVPL
CCCCCHHHHHHHHCCCEECCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCE
KCDLCREEGEPACVAGCPNRALVFEERRAGA
EEHHHHCCCCCEEEECCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CO; H2O; A

Specific reaction: CO + H2O + A = CO2 + AH2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644755 [H]