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The map label for this gene is nasB [H]

Identifier: 83590138

GI number: 83590138

Start: 1329811

End: 1331040

Strand: Reverse

Name: nasB [H]

Synonym: Moth_1290

Alternate gene names: 83590138

Gene position: 1331040-1329811 (Counterclockwise)

Preceding gene: 83590139

Following gene: 83590137

Centisome position: 50.63

GC content: 60.41

Gene sequence:

>1230_bases
ATGAACTACGTCCTTATCGGCAATTCCGTAGCTGCTGTCAACGCCGTGGCCGGTATCCGGGAATACGACCGGGAAGGGGA
TATCACCATCGTGTCGGCGGAGAACTACTACGCCTATGGCCGTCCCCTTATTTCTTACTGGCTGGAGCAGCGGGTTAACG
AGAAAAATCTGCCTTACCGTCCCGAAACCTTCTTTGCTCGAAACAAGGTCCGGGTGCTCCTTGGCCGCCGGGCAGTGCGC
CTGGACCCGGAGGCCAGGCAGGTTTTCCTGGATAACGGCGAGTCCCTGCCCTATGACCGCCTTCTTCTGGCCACCGGAGG
CAGGCCCTTTTTGCCCTCCATTAACGGCCTGACCCCGGAAAACCACTACACCTTTATGACCTATGACGACGCCCGCCGCC
TGGAAAAGGCGGCCTCCCCCGGGCGGGAAGCCGTCATTCTGGGGGCCGGGCCAACGGGCCTCAAGGCCATGGAGAGCCTG
GTCCGGCGAGGGGTAAAGGTTACGCTGGTCGAACTGGCCGACCGGATATGGGCCCCGGCCCTGGATCCGGAGGCAGCCTC
CATGGTGGCCGGTTTCCTGCAAGATAGGGGTGTAAATATCTATTTGAACGATACCCTGGATGGCGTCCGCCGGGGAGACA
ACGGACGCCTCGACCTGGAACTCAAATCAGGTCGCCGGCTGGCCGCAGATATCCTGGTCGTGGCTATAGGCGTGCGGCCC
AATGTCGAACTCCTCCAGGATCTGCCCGGCGCAACCATAAACCGCGGCGTGGTAGTAGGGGCGGATCTCAGCACCGGTCT
GCCCGGCGTTTATGCCGCCGGGGATGTAGTGGCCGGCAACCCGCCCCTTTTGCCCCATGCCGCTATCCAGGGTAAGATAG
CCGGCCGCAACATGGCAGGGGGCCGGGAAACCTATAAGCCAATACCGCCTTATAACGCCCTGGGTATCCTGGGGTTGCAC
ATTATCAGCATAGGTTTAAGCGCCGCTGGCGAGGGTTACGAAATCCTGCAGGAAGCCGATCCTGCCGCCCGGGTTTACCG
CAAGCTGGTGCTGCAGGATAACCGCCTGGTAGGCGGGCTGCTGATTAATAAAATCGACCGGGCCGGCATCTACCGCTATC
TAATCGAGGAAGGCATCGAGGTAACGTCCTTTAAAGATCGACTGCTGCAGCCGGATTTCGGTTTATTGAGCCTCCCGGAC
ACCATCTGGCAGCAGCAGCTGGCAAAGTGA

Upstream 100 bases:

>100_bases
TACCTTTAAAGTGCGACCTCTGCCGGGAAGAAGGCGAACCGGCCTGCGTGGCCGGTTGCCCCAACCGGGCCCTGGTTTTT
GAAGAGAGGAGGGCAGGGGC

Downstream 100 bases:

>100_bases
GGTGACGGGTGTGGAAGTAGTAAATGCCAGGGGCCTGTCTTACCAGGCCTTAAACGAAACCGTTCGCTCCCTCCTGGATA
ATGGGCAGCGGGAACTGGTC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NAD; reduced flavorubredoxin; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MNYVLIGNSVAAVNAVAGIREYDREGDITIVSAENYYAYGRPLISYWLEQRVNEKNLPYRPETFFARNKVRVLLGRRAVR
LDPEARQVFLDNGESLPYDRLLLATGGRPFLPSINGLTPENHYTFMTYDDARRLEKAASPGREAVILGAGPTGLKAMESL
VRRGVKVTLVELADRIWAPALDPEAASMVAGFLQDRGVNIYLNDTLDGVRRGDNGRLDLELKSGRRLAADILVVAIGVRP
NVELLQDLPGATINRGVVVGADLSTGLPGVYAAGDVVAGNPPLLPHAAIQGKIAGRNMAGGRETYKPIPPYNALGILGLH
IISIGLSAAGEGYEILQEADPAARVYRKLVLQDNRLVGGLLINKIDRAGIYRYLIEEGIEVTSFKDRLLQPDFGLLSLPD
TIWQQQLAK

Sequences:

>Translated_409_residues
MNYVLIGNSVAAVNAVAGIREYDREGDITIVSAENYYAYGRPLISYWLEQRVNEKNLPYRPETFFARNKVRVLLGRRAVR
LDPEARQVFLDNGESLPYDRLLLATGGRPFLPSINGLTPENHYTFMTYDDARRLEKAASPGREAVILGAGPTGLKAMESL
VRRGVKVTLVELADRIWAPALDPEAASMVAGFLQDRGVNIYLNDTLDGVRRGDNGRLDLELKSGRRLAADILVVAIGVRP
NVELLQDLPGATINRGVVVGADLSTGLPGVYAAGDVVAGNPPLLPHAAIQGKIAGRNMAGGRETYKPIPPYNALGILGLH
IISIGLSAAGEGYEILQEADPAARVYRKLVLQDNRLVGGLLINKIDRAGIYRYLIEEGIEVTSFKDRLLQPDFGLLSLPD
TIWQQQLAK
>Mature_409_residues
MNYVLIGNSVAAVNAVAGIREYDREGDITIVSAENYYAYGRPLISYWLEQRVNEKNLPYRPETFFARNKVRVLLGRRAVR
LDPEARQVFLDNGESLPYDRLLLATGGRPFLPSINGLTPENHYTFMTYDDARRLEKAASPGREAVILGAGPTGLKAMESL
VRRGVKVTLVELADRIWAPALDPEAASMVAGFLQDRGVNIYLNDTLDGVRRGDNGRLDLELKSGRRLAADILVVAIGVRP
NVELLQDLPGATINRGVVVGADLSTGLPGVYAAGDVVAGNPPLLPHAAIQGKIAGRNMAGGRETYKPIPPYNALGILGLH
IISIGLSAAGEGYEILQEADPAARVYRKLVLQDNRLVGGLLINKIDRAGIYRYLIEEGIEVTSFKDRLLQPDFGLLSLPD
TIWQQQLAK

Specific function: Required for nitrate assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=345, Percent_Identity=28.1159420289855, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI65787454, Length=345, Percent_Identity=28.1159420289855, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI226437568, Length=345, Percent_Identity=28.1159420289855, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI22202629, Length=379, Percent_Identity=27.1767810026385, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4757732, Length=379, Percent_Identity=27.1767810026385, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI91199540, Length=300, Percent_Identity=28, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1789065, Length=307, Percent_Identity=29.3159609120521, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788892, Length=275, Percent_Identity=31.6363636363636, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789765, Length=392, Percent_Identity=25, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082354, Length=246, Percent_Identity=31.3008130081301, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789915, Length=236, Percent_Identity=30.0847457627119, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=394, Percent_Identity=23.8578680203046, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24639257, Length=343, Percent_Identity=25.9475218658892, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281359715, Length=343, Percent_Identity=25.9475218658892, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI281359713, Length=343, Percent_Identity=25.9475218658892, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24639250, Length=343, Percent_Identity=25.9475218658892, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI18543267, Length=343, Percent_Identity=25.9475218658892, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24639252, Length=343, Percent_Identity=25.9475218658892, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24585130, Length=325, Percent_Identity=26.7692307692308, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24581020, Length=385, Percent_Identity=27.2727272727273, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28573993, Length=385, Percent_Identity=27.2727272727273, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585384, Length=199, Percent_Identity=30.6532663316583, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358499, Length=262, Percent_Identity=29.3893129770992, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640553, Length=231, Percent_Identity=28.1385281385281, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24640549, Length=231, Percent_Identity=28.1385281385281, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 44646; Mature: 44646

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYVLIGNSVAAVNAVAGIREYDREGDITIVSAENYYAYGRPLISYWLEQRVNEKNLPYR
CCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCEECCCHHHHHHHHHHCCCCCCCCC
PETFFARNKVRVLLGRRAVRLDPEARQVFLDNGESLPYDRLLLATGGRPFLPSINGLTPE
CCHHHHCCCEEEEECCCEEEECHHHHEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC
NHYTFMTYDDARRLEKAASPGREAVILGAGPTGLKAMESLVRRGVKVTLVELADRIWAPA
CCEEEEEEHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEHHHHHHHHCCCC
LDPEAASMVAGFLQDRGVNIYLNDTLDGVRRGDNGRLDLELKSGRRLAADILVVAIGVRP
CCHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCEEEEEECCCCCCEEEEEEEEECCCC
NVELLQDLPGATINRGVVVGADLSTGLPGVYAAGDVVAGNPPLLPHAAIQGKIAGRNMAG
CHHHHHHCCCCCCCCCEEEECCCCCCCCCCEECCCEECCCCCCCCCHHHCCEECCCCCCC
GRETYKPIPPYNALGILGLHIISIGLSAAGEGYEILQEADPAARVYRKLVLQDNRLVGGL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEHHH
LINKIDRAGIYRYLIEEGIEVTSFKDRLLQPDFGLLSLPDTIWQQQLAK
HHHHHHHHHHHHHHHHCCCEEECHHHHHCCCCCCCEECCHHHHHHHHCC
>Mature Secondary Structure
MNYVLIGNSVAAVNAVAGIREYDREGDITIVSAENYYAYGRPLISYWLEQRVNEKNLPYR
CCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCEECCCHHHHHHHHHHCCCCCCCCC
PETFFARNKVRVLLGRRAVRLDPEARQVFLDNGESLPYDRLLLATGGRPFLPSINGLTPE
CCHHHHCCCEEEEECCCEEEECHHHHEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC
NHYTFMTYDDARRLEKAASPGREAVILGAGPTGLKAMESLVRRGVKVTLVELADRIWAPA
CCEEEEEEHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEHHHHHHHHCCCC
LDPEAASMVAGFLQDRGVNIYLNDTLDGVRRGDNGRLDLELKSGRRLAADILVVAIGVRP
CCHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCEEEEEECCCCCCEEEEEEEEECCCC
NVELLQDLPGATINRGVVVGADLSTGLPGVYAAGDVVAGNPPLLPHAAIQGKIAGRNMAG
CHHHHHHCCCCCCCCCEEEECCCCCCCCCCEECCCEECCCCCCCCCHHHCCEECCCCCCC
GRETYKPIPPYNALGILGLHIISIGLSAAGEGYEILQEADPAARVYRKLVLQDNRLVGGL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEHHH
LINKIDRAGIYRYLIEEGIEVTSFKDRLLQPDFGLLSLPDTIWQQQLAK
HHHHHHHHHHHHHHHHCCCEEECHHHHHCCCCCCCEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; oxidized flavorubredoxin; O2 [C]

Specific reaction: NADH + oxidized flavorubredoxin + O2 = NAD + reduced flavorubredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]